81 results about "Streptomycin sulphate" patented technology

The invention relates to a method for comprehensively preventing and controlling tobacco bacterial wilt. The method comprises the following steps of: mainly applying a biological anti-microbial agent Qing weisan; supplementing a chemical agent, namely 72 percent agricultural streptomycin sulphate soluble powder; clearing away the refuse in a tobacco field in winter; and taking tillage measures of deep furrow drainage in a field period. The comprehensive preventing and controlling treatment measures have the practical application value of controlling diseases and promoting production and reduce the tobacco loss. Field experiments show that the disease incidence and the disease index of the tobacco bacterial wilt in the comprehensive preventing and controlling field are obviously lower than those of a comparison field, and the relative preventing effect reaches 81.2 percent.

The invention relates to a half artificially fed cabbage moth feed and a preparation method thereof. The feed is prepared by mixtures A, B and C, agar and distilled water, wherein the mixture A comprises casein, sucrose, malt meal and wild cabbage powder; the mixture B comprises cellulose, para hydroxybenzoic acid, potassium sorbate and Watkins salt; the mixture C comprises vitamin C, streptomycin sulphate, propyl gallate, vitamin B complex, edible vegetable oil, potassium hydroxide and formalin. The preparation method comprises the following steps: components of the mixture A, B and C are respectively put into a container; 5 / 6 of the distilled water and the agar powder are added into the container for heating and stirring to thawing; the mixture A is poured into the agar liquid and stirred until uniformity; the mixture B is added and stirred until uniformity; the rest distilled water is added, and the mixture C is added when the temperature is decreased to 70 DEG C, and then stirred until uniformity; the obtained product is filled into moth feeding cups; after the surface moisture is dried, the cups are put into a refrigerator for storage. The preparation method and the feed of the invention are scientific, simple, and easy to standardize propagation the cabbage moth indoors with large scale; the cabbage moth fed with the feed has high larva survival rate, orderly development and sound growth; and the descendants of the cabbage moth have high hatching rate.

The invention discloses a disinfectant for treating bacterial fruit rot of seedless watermelons and a shell-breaking-free accelerating bud-forcing method for seedless watermelons, wherein the disinfectant contains 2% Kasumin liquid 300-400 fold and 72% agricultural streptomycin sulphate 1000-1500 fold; and the bud-forcing method comprises: soaking seeds in water of 55 DEG C for 30min, then in the disinfectant for 3h, and in formaldehyde 100 fold for 30min after washed; flushing the seeds with saturated limewash until no mucus on seed surfaces, then flushing the seeds with clear water until no peculiar smell, and removing pericarp and wizened seeds; drying until the surfaces of the seeds turn white; wrapping the seeds with cotton cloth, placing the seeds into a thermotank, accelerating germination at 35 DEG C for 2h, and accelerating germination at 32 DEG C for 12-15h; washing the seeds with water of 35 DEG C, drying and dehydrating the seeds, airing the seeds until the surfaces have no obvious water, and waiting for germination in the thermotank of 32 DEG C. The disinfectant and the method can reduce probability for the seedless watermelons to infect bacterial fruit rot below 2 parts per thousand, have no need of artificial shell breaking, can reduce bud-forcing cost of the seedless watermelons, and raises a seedling rate of the seedless watermelon seeds.

The invention discloses a preparation method for applying autogenous skin fibroblasts to beautifying and anti-aging. The method includes the following steps that the human autogenous abdominal skin is cut and put into PBS containing ampicillin and streptomycin sulphate to be rinsed, and the rinsed human autogenous abdominal skin is added into a DMEM containing collagenase and hyaluronidase and is placed overnight; PBS digestive juice containing pancreatin and EDTA is added, cells are collected in a centrifugal mode, and the collected cells are eluted with a DMEM and then cultured in an open mode; the cells are subcultured in a certain proportion and prepared into cell suspension; the cell suspension is taken and transferred into a centrifugal tube, and autogenous skin fibroblasts are extracted in a centrifugal mode; various factors are added into the autogenous skin fibroblasts, and a mixture is obtained and applied to beautifying and anti-aging. According to the method, the biological effects of the autogenous skin fibroblasts are utilized, the nutritional factors are added, and the mixture is better stored and used in time within the biological shelf life and can perform the optimal beautifying effects such as repair, regeneration, filling, freckle removal and oxidation resistance on the skin.

The invention discloses a nutrient solution for maturation of oocyte in vitro and a method for culturing oocyte using the nutrient solution, and relates to a nutrient solution for nutrient solution for oocyte and a method for culturing the oocyte with the nutrient solution. The nutrient solution and the culturing method using the nutrient solution solve the problem that the viability of oocyte culturing in vitro is low. The nutrient solution for maturation of oocyte in vitro is composed of normal nutrient solution of oocyte, vitamin B12, fetal calf serum, FSH, LH, 17 betas estradiol, sodium pyruvate, EGF, penicillin and streptomycin sulphate. The oocyte is placed into the utrient solution for maturation of oocyte in vitro, the maturation culture on the oocyte lasts for 20-24 hours in the environment in which the volume concentration of CO2 is 5%, and a mature oocyte is acquired. The nutrient solution for maturation of oocyte in vitro can decrease stress injury caused by the external environment, the cytoplasm maturing of the oocyte is promoted, and the parthenogenetic activation of the matured oocyte matured in vitro and the embryos developmental potential after being fertilized are improved.

The invention discloses a seed coating for konjac seedling seeds. The seed coating comprises a seed coating inner layer and a seed coating outer layer which are sequentially coated outside the seeds from the inside to the outside, wherein the seed coating inner layer comprises the following raw materials in percentage by weight: 4.5% of fertilizer, 0.15% of plant hormones, 5.1% of carbendazim, 0.16% of streptomycin sulphate, 1.35% of film-forming agent, 1.35% of dispersing agent, 0.85% of anti-caking agent, 5.1% of suspending agent, 9.3% of filling agent, 0.85% of colouring agent and the balance water; the seeds are A.muelleri seedling seeds; and the seed coating outer layer comprises a water-retention layer and a heat-insulation layer which are sequentially coated outside the seed coating inner layer. The technical problem to be solved by the invention is to provide a seed coating for konjac seedling seeds, and a preparation method thereof. The seed coating is capable of improving the vitality of the A.muelleri seeds, good in seedling growth effect, high in yield and good in disease and pest prevention effect.

The present invention relates to a treating method for sterilizing the explants, which have bacteria in tissues. The treating method is the plant material of the explants are implemented with the common treatment and the part of the fringe of the explants whose color changes with the sterilization is cut and then the explants are cut into small pieces of 1 to 3 cm for use; the method is characterized in that the explants are separately transplanted into different culture media of different concentrations; 8 to 10 explants are transplanted in one triangular bottle and the fringe of every explant contact the culture media; the explants are put in a culture room for cultivation. The method of the present invention is characterized by simple operation; the three antibiotics of carbenicllin, streptomycin sulphate and nystatin have obvious effect on the sterilization of the explants which not only effectively constrain the bacteria (100 percent when the three antibiotics are injected with the amount of 500 plus 100 plus 1000mg an hour) but also basically control the growth of fungus.

The invention relates to the technology of pesticide blending, in particular to a preparation compounded from an antibiotic bactericide particularly referring to streptomycin sulphate and one of the following methoxy-acrylate bactericides: kresoxim-methyl, azoxystrobin, enestroburin, SYP-1620, metominostrobin, Pyraclostrobine, Phenothrin and the like, and the application thereof. In the bactericide, the streptomycin sulphate and the active ingredient B are compounded to expand a bactericidal spectrum and achieve synergy, thereby obtaining undesirable bactericidal effect. The bactericide is used in the mode of a composite, thereby not only improving prevention efficiency, but also lowering use cost and improving the utilization ratio of effective ingredients.

The invention belongs to the technical field of agricultural chemicals, particularly relates to the field of plant-sourced bactericides and specifically provides a plant-sourced bactericide for controlling bacterial wilt of tobacco. The main active constituents of the plant-sourced bactericide for controlling bacterial wilt of tobacco are amaranth crude extract and sterile water, wherein each milliliter of the plant-sourced bactericide contains 0.17-0.5 mg of the amaranth crude extract. The plant-sourced bactericide for controlling bacterial wilt of tobacco has the advantages as follows: 1, the raw material of the amaranth crude extract is convenient to obtain and the amaranth crude extract can be produced industrially and can be applied by farmers; 2, the effect of the plant-sourced bactericide is obvious and the median inhibitory concentration EC50 is 0.0415 mg / mL; the effect of the plant-sourced bactericide for controlling bacterial wilt of potted tobacco is 77.00-90.29 percent andis obviously higher than the effects of streptomycin sulphate and other conventional plant-sourced bactericides for controlling bacterial wilt of tobacco; and 3, the amaranth crude extract is high inefficiency, low in toxicity, nuisanceless, and safe to environment.

The invention discloses a method for constructing a slow release system for medicament on surface of a medical titanium alloy implant. The system is characterized in that the method comprises the following steps: a solvent blending method is used for grafting maleic anhydride to polylactic acid in order to prepare maleic anhydride grafted polylactic acid; MPLA microballoons containing streptomycin sulphate is prepared by a self-assembly balling method; surface amination of titanium alloy is carried out by surface grafting modification, so that the surface of titanium has positive charges, a layer by layer assembly technology is used, the MPLA microballoons and PEI are used as negative and positive ions respectively, static adsorption is used, and an SS-MPLA microballoon / PEI multilayer antibacterial film is constructed on the surface of the titanium material. When the multilayer antibacterial film on the surface of the implant in vivo is degraded, streptomycin sulphate is slowly released, so that long-term bacteriostasis effect is realized, and the system has the effects of good compatibility, high stability, firm combination, and long slow release time.

The invention discloses a chromosome preparation method, as well as a required culture medium and a preparation method thereof, and is used for solving karyotype analysis problem of chromosome. The culture medium consists of RPMI (Roswell Park Memorial Institute) 1640, heparin sodium, HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid), L-glutamine, NaHCO3, benzylpenicillin potassium, streptomycin sulphate, bovine serum and phytohemagglutinin (PHA). The detection method comprises the following steps: implanting 0.3 to 0.4ml of human peripheral blood into the culture medium; adding colchicinamide in 2-4 hours before culture is terminated to realize that the cell is terminated in anaphase; culturing the cell after 68 to 72 hours to harvest the cell; performing hypotonicity for 40 minutes, three times of fixation, banding, dyeing and other treatments; and performing chromosome analysis under a microscope to determine whether the peripheral blood supplier has a phenomenon of chromosome abnormality. The culture medium disclosed by the invention has convenience for use, simpleness in operation, low cost and low patient detection fee, and is suitable for genetic diagnosis, infertility and prenatal diagnosis in each level of hospitals.

The invention discloses a special medicine fertilizer for konjak. The special medicine fertilizer is characterized by being formed by the following components in mass percentage: 15-27% of a base material and 73-85% of a medicine fertilizer. The medicine fertilizer is formed by the following raw materials in parts by weight: 16-21 parts of urea, 22-28 parts of monoammonium phosphate, 34-36 parts of potassium sulfate, 0.08-0.1 part of nitryl fulvic acid salt, 0.06-0.08 part of agricultural streptomycin sulfate and 0.1-0.12 part of chlorothalonil. When the special medicine fertilizer is used, 50-80kg of the medicine fertilizer is applied to each 666.7 m<2>. The medicine fertilizer disclosed by the invention can improve the anti-disease capability of a konjak plant and prevents main konjak disease bacteria from invading konjak plants and corms; the medicine fertilizer ensures the sufficient requirements of the growth of the konjak on the a main fertilizer; and the medicine and the fertilizer are combined so that the special medicine fertilizer prevents diseases, balances nutrition, improves the soil, improves the medicine fertilizer effects, enhances the konjak resistance, accelerates the growth of the konjak and has the very obvious social, economic and ecological comprehensive benefits.

The invention discloses a streptomycin sulfate detection method. The method is implemented through a specific reaction of a chemical fluorescent probe with streptomycin sulfate and comprises steps as follows: 1) dissolving the chemical fluorescent probe in an organic solvent to obtain a solution A, and dissolving alkali in water to obtain a solution B; 2) dissolving the streptomycin sulfate in water to prepare a series of streptomycin sulfate aqueous solutions with different concentrations, that is, standard solutions; 3) mixing and oscillating the solution A and the solution B with the standard solutions with the different concentrations respectively, leaving the mixtures to stand for layering, transferring water layers, measuring the fluorescence intensity, and establishing a correlation curve about the fluorescence intensity and the concentrations of the standard solutions; 4) mixing and oscillating the solution A and the solution B with a to-be-detected sample solution, leaving the mixture to stand for layering, transferring a water layer, measuring the fluorescence intensity, comparing the curve with the curve established in Step 3), and calculating the content of the streptomycin sulfate in a sample.

The invention relates to an ultralow temperature cryopreservation method of macaca primate semen, belonging to an animal breeding method and comprising the following steps: dissolving Tris, TES, lactose, glucose, raffinose, sodiumpenicillinG, streptomycin sulphate and the like in water, centrifuging, taking supernate, preparing the supernate into semen diluent, adding glycerol to prepare deicing fluid, diluting the semen by using diluent, cooling the temperature to 4 DEG C, mixing with the dicing fluid precooled to 4 DEG C with the same volume, subpackaging into frozen straws, cooling by utilizing liquid nitrogen steam, and placing in liquid nitrogen at the temperature of minus 196 DEG C for preservation. When in use, the semen is defrosted and revived. The method in the invention can improve the revived motion range, the survival time, the plasma membrane and acrosome completeness and the fertility rate of the frozen sperm of the macaca primate.

The invention discloses an animal-origin-free antifreezing solution and a cryopreservation method of the animal-origin-free antifreezing solution on seminal liquid of a machin. According to the antifreezing solution, 0.2 g of Tris 1.2 g of TES, 2 g of lactose, 2 g of glucose, 0.2 g of raffinose, 10,000 IU of penicillin G sodium salt and 5 mg of streptomycin sulphate are dissolved in 80 mL of Milli-Q ultrapure water in sequence, the Milli-Q ultrapure water is added after even mixing for achieving the constant volume of 100 mL, pH is adjusted to be 7.0 to 7.2 through 1 N of NaOH or 1 N of HCl, a diluent is prepared, glycerinum is added to the diluent, and the 2* antifreezing solution is prepared. The risk of introducing pathogenic factors in an external fertilization or artificial fertilization system is completely eradicated, a sperm low-temperature freezing injury mechanism and a freezing protection mechanism can be understood better, and quality control over the antifreezing solution can be carried out easily.

The invention discloses a boar frozen semen diluted powder, which is characterized in that: the diluted powder is composed of D-glucose, trisodium citrate, EDTA sodium salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Typell), trishydroxymethylaminomethanol (Tris), citric acid, cysteine, OEP (amino-sodium dodecyl sulfate), SDS (sodium dodecyl sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, and N-acetyl-D-aminoglucose. The boar frozen semen diluted powder provided by the invention improves the fertilization rate and litter size of boar frozen semen, simultaneously can improve the product safety, reduces the risk of semen infection caused by animal derived components in the diluent, and fills the domestic blank in the field, thus having far-reaching significance.

The invention relates to a capsicum multifunctional insecticide-fertilizer. The capsicum multifunctional insecticide-fertilizer is characterized by comprising, by weight, 33.1 to 33.4 parts of mushroom slag, 32 to 34 parts of urea, 19.4 to 20 parts of potassium sulfate, 7.1 to 7.5 parts of monoammonium phosphate, 2 to 2.5 parts of sodium polyacrylate, 0.6 to 0.8 part of copper sulphate, 0.4 to 0.5 part of potassium permanganate, 0.35 to 0.4 part of aluminum Ridomil, 0.29 to 0.3 part of thiophanate methyl, 0.29 to 0.3 part of carbendazim, 0.18 to 0.2 part of agricultural streptomycin sulphate and 0.08 to 0.1 part of compound sodium nitrophenolate. The insecticide-fertilizer provided by the invention integrates trophic action of mushroom slag, urea, potassium sulfate and monoammonium phosphate, the water retention effect of sodium polyacrylate, the disease prevention effect of copper sulphate, potassium permanganate, aluminum Ridomil, thiophanate methyl, carbendazim and agricultural streptomycin sulphate and the growth-promoting effect of compound sodium nitrophenolate together to overcome the problems of unbalance of soil nutrients, reduced water retention capability of soil, severe soil borne diseases and the like of capsicum. The capsicum multifunctional insecticide-fertilizer is applied to fields like production of capsicum fertilizers, supplement of soil nutrients during capsicum plantation, prevention and treatment of capsicum diseases, etc.

The invention discloses a formula of a novel agentia for preventing and treating mango bacterial black spot, and a preparation method of the novel agentia. The formula comprises 1000,000 units of streptomycin sulphate with the concentration of 7.50 mu g / mL, 20% thiediazole copper SC with the concentration of 61.90 mu g / mL, and the ratio is 3:2. The preparation method comprises the following steps: selecting a single bactericide necessary for compounding, preparing a bacterium suspension, and evaluating the antibacterial effect inside a compounding agentia room. Due to the adoption of the agentia prepared according to the formula, the defects that the single agentia is not ideal in prevention effect and single in prevention and treatment spectrum, and a mango bacterial black spot pathogenic bacterium is easy to have medicine resistance are overcome, and not only is the prevention effect good, but also both treatment and protection functions are achieved, the prevention and treatment spectrum is widened, the medicine resistance is delayed, and the prevention and treatment cost is low. A toxic containing medium method is adopted, a plurality of ordinary bactericides are compounded by using a mixing method according to a ratio, the purpose of selecting a compounded agentia with high efficiency, low toxicity and both treatment and protection function is achieved, the mango bacterial black spot is effectively prevented and treated, and meanwhile the medicine resistance of the pathogenic bacterium is delayed.

A case of goose seminal fluid chill diluent and application method thereof belongs to animal breed technique field, the reagent case including: component I: sodium glutamate 0.4-0.6, potassium dihydrogen phosphate 1.4-2.2, dipotassium hydrogen phosphate 0.2-0.5, homocycteine 0.0006-0.01, fructose 0.9-1.2, inositol 0.04-0.08, raffinose 0.5-0.8, sodium oxacillin 8-12, streptomycin sulphate 0.07-0.15, calcium chloride 0.01-0.02, 3-carboxymethylaminomethane 0.1-0.3; component II: double distilled water 100; component III: protamini sulfas 0.04-0.06 g; component IV: DMA 4.5-5.5, glycol: 4.5-5.5 ml. Useful effects: using components of 3-carboxymethylaminomethane and protamini sulfas and quantifying accurately. Compounding each component of goose seminal fluid chill diluent as packing type of case firstly. The method is simple and easy to operate, and improve applying effects of the case.

The invention discloses umbilical cord mesenchymal stem cell transportation protective liquid which is prepared from ingredients in volume percent: 1% to 3% of heparin sodium solution, 1% to 3% of streptomycin sulfate solution, 80% to 96% of DMEM culture medium water solution, 1% to 2% of sofren injection and 1% to 2% of xiangdan injection. According to the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention, different ingredients of transportation protective liquid is utilized to perform storage experiments and anticoagulation experiments on umbilical cord mesenchymal stem cells having the same source; after being treated for the same time, statistics are performed on survival rates of all the treated umbilical cord mesenchymal stem cells; results show that the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention can ensure a higher cell survival rate; the umbilical cord mesenchymal stem cell transportation protective liquid is prepared from the sofren injection and the xiangdan injection, so that an anticoagulation effect of stem cells is ensured in compatible use, the heparin sodium use amount is simultaneously reduced, side effects caused by the heparin sodium is reduced, and safety of the umbilical cord mesenchymal stem cell transportation protective liquid disclosed by the invention is improved.

The invention discloses a high efficient biological composite alga fertilizer, which is composed of the following raw materials by weight: 100 kilograms of alga liquid, 0.1 to 0.3 kilogram of composite amino acid, 0.8 to 0.9 kilogram of biochemical fulvic acid, 20 to 30 kilograms of urea, 3 to 6 kilograms of salicylic acid, 0.3 to 0.6 kilogram of streptomycin sulphate, 2 to 3 kilograms of calcium superphosphate, 0.5 to 0.8 kilogram of potassium sulfate, and 3 to 5 kilograms of boric acid. The provided fertilizer can increase the content of organic substances in soil, achieves the goals of fertilizing the soil, obtaining a stable and high output, and increasing the output and incomes, and creates a good agricultural ecosystem. The fulvic acid can be easily dissolved in water, so no flocculation or precipitation is generated after compounding the fulvic acid with various chemical fertilizers. The nutrient content is high, the fertilizing effect is quick, the utilization of the nutrients in the fertilizer is high in the current quarter, and the economic and social profits are good.

A plant fungicide for prevention and control of rice diseases comprises at least one adjuvant and streptomycin sulfate and 3-O-(N-5-(phenyl)sulfydryl)-1,3,4-thiadiazole-2-radical)-acetamide-2-radical)-3', 4', 5', 5, 7-pentamethoxyl myricetin (A2) which are of the mass ratio being 1 : (0.25 to 0.35). The dosage form of the plant fungicide for prevention and control of rice diseases is selected fromwettable powder, suspending agent and soluble powder. The total mass of streptomycin sulfate and the compound (A2) accounts for 15-30% of the total mass of the plant fungicide. If the plant fungicideis of the form of wettable powder, its adjuvant refers to the adjuvant adaptable to the wettable powder and comprising dispersant, a wetting agent, a suspending agent and a carrier; preferably, the dispersant refers to nekal BX, the wetting agent refers to lauryl sodium sulfate and the suspending agent refers to sodium carboxymethylcellulose.

The invention relates to a semi-artificial diet preparing and breeding method of tetranychus cinnabarinus. The method is characterized in that: any four or more than four of saccharose, beer yeast powder, fresh yolk, multi-vitamins, KH2PO4 and Na2HPO4 are taken to form a mixture of more than 10 g and less than 30 g, then 164 g of water, 0.005 g of streptomycin sulphate and 0.2 g of sorbic acid are added into the mixture, the volume is metered to 200 ml, then 30 ml of an aqueous liquid ofkidney beanleavesis added to form a semi-artificial diet for the tetranychus cinnabarinus, and the semi-artificial diet is used in the method for breeding the tetranychus cinnabarinus.A novel liquid is provided for the maintenance and amplification of the tetranychus cinnabarinuspopulation, the tetranychus cinnabarinuspopulation is maintained and reproduces within a certain period of time, and particularly, the method is used to store the food for thetetranychus cinnabarinus for a long period of time.

The invention discloses a culture medium for quickly separating and identifying banana fusarium oxysporum. The culture medium is a group of composites and is characterized in that: every 1,000 milliliters of water solution comprise the following components: 180-220g of culture medium potato, 0.4-0.6g of brochantite, 0.5-0.7g of epsom salt, 0.08-0.12g of monopotassium phosphate, 18-20g of agaragar, 10-15 milliliters of 95 percent alcoholic solution, 2.5-3.0g of 75 percent dexon crystalline powder and 0.5-0.8g of streptomycin sulphate. The culture medium has the advantages of reasonable raw material ingredients, vigorous growth of pathogenic bacteria of fusarium oxysporum, formation of a white bacterial colony, remarkable bacterial colony characteristic and high occurrence rate of the bacterial colony; a flat plate can be manufactured inversely without performing high-pressure steam sterilization on the culture medium, a culture dish does not need high-pressure steam sterilization after being cleaned and dried, and sterile working is not required during inoculation; and the raw materials are readily available, the effect is enhanced, and the cost is saved.

A method for preventing and treating tomato bacterial wilt comprises the steps that firstly, tomatoes are planted based on a traditional method, and when transplanted seedlings turn green, a root-pouring agent A is poured to roots, wherein 0.3-0.5 kg of the root-pouring agent A is poured to each plant; secondly, if diseased or infected plants are found, a root-pouring agent B is poured to the roots of the diseased or infected plants and the roots of healthy plants around the diseased or infected plants every 5-7 days 2-3 times continuously, and a foliage spray agent C is used for mist spray, wherein 0.5-1 kg of the root-pouring agent B is poured to each plant. The root-pouring agent A is prepared from copper sulfate and quick lime; the root-pouring agent B is prepared from quick lime, copper sulfate and urea; the foliage spray agent C is prepared by evenly stirring and mixing 3000-fold liquid of 72% agricultural streptomycin sulfate, 3000-fold liquid of 99% hymexazol wettable powder and 600-fold liquid of tianda-2116 with 500-fold liquid of 20% bismerthiazol wettable powder, 4000-fold liquid of atonik, 200-fold liquid of brown sugar and 500-fold liquid of urea.

The invention discloses a culture solution for improving maturity quality and development potential of in-vitro cultured porcine oocytes and its cultivating method. The culture solution is prepared from, by weight, 3-7 mu mol / L of forskolin, 3-7 mu mol / L of Roscovitine, 8-12g / L of TCM-199, 1-4g / L of NaHCO3, 0.4-0.7g / L of D-glucose, 0.05-0.15g / L of sodium pyruvate, 0.05-0.1g / L of penicillin sodium, 0.03-0.07g / L of streptomycin sulphate, 1-4g / L of polyving akohol, 0.3-0.7mu g / mL of luteotropin, 0.3-0.7mu g / mL of follicle-stimulating hormone, 8-12ng / mL of epidermal growth factor, 0.3-0.7mmol / L of L- cysteine, and 8-12% of follicular fluid. The culture solution has the advantages of improving cleavage rate, blastaea number and blastaea cell population of porcine oocytes, and effectively improving the development potential of the in-vitro cultured porcine oocytes; so the culture solution has great application prospect.

The invention discloses a prevention method for plant bacterial canker. The prevention method comprises the following steps of taking and dissolving veterinary-use florfenicol liquid under a water bath condition, adding veterinary-use gentamicin, 98% veterinary-use streptomycin sulphate and diethyl aminoethyl hexanoate, diluting with 15kg of water, and evenly stirring to obtain bactericide liquid; after a plant cultivated in a sunlight greenhouse suffers from the bacterial canker, thoroughly watering the plant before the bactericide liquid is sprayed, spraying the bactericide liquid in a sunny day, sealing the greenhouse after the bactericide liquid is sprayed, cleaning the greenhouse film of the greenhouse with dry cloth or a sponge for removing dust, improving the indoor temperature of the sunlight greenhouse to 53-55DEGC, and then, closing the greenhouse; repeatedly spraying the bactericide liquid, and closing the greenhouse one time; after water is added into the bactericide liquid to carry out dilution, carrying out root irrigation on the plant suffering from the bacterial canker. The prevention method has the advantages of scientific compatibility, good sterilization effect and obvious effect on preventing and curing diseases including plant bacterial canker, so that the plant is free from the infringement of bacteria in a whole growth process and does not generate drug resistance.

The invention discloses a chromosome culture medium for quickly harvesting metaphase cells. The chromosome culture medium for quickly harvesting the metaphase cells comprises the following active ingredients: an RPMI 1640 powder culture medium, calf serum, penicillin sodium, streptomycin sulphate, sodium bicarbonate and phytohemagglutinin(PHA), wherein the contents of various active ingredients are respectively as follows: 10.4 g / L of the RPMI 1640 powder culture medium, 100 ml / L of the calf serum, 400 to 600 mu l / L of the penicillin sodium, 400 to 600 mu l / L of the streptomycin sulphate, 2 g / L of the sodium bicarbonate and 100 to 120 mg / L of the phytohemagglutinin(PHA). The effects of different components and the PHA are fully played; a large amount of cells in a metaphase can be obtained; chromosome nuclear type analysis and clinical diagnosis are conveniently performed; meanwhile, the chromosome culture medium can support quickly and effectively harvesting the metaphase cells and shorten the processing time of colchicines, so that the time required for preparing chromosomes is shortened; help is provided for performing the chromosome nuclear type analysis and genetic diagnosis largely.

The invention relates to a saliva preserving fluid and a preparation method and application thereof. The saliva preserving fluid is prepared from 1-10 mmol / L of EDTA, 1-10 mmol / L of ethylene glycol-bis(2-aminoethyl ether)tetraacetic acid, 30-70 micrograms / mL of sodium benzyl penicillinate, 30-70 micrograms / mL of streptomycin sulphate, 30-70 mmol / L of Tris-HCl, 1-25 mmol / L of 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid, 30-70 mmol / L of N-tris(hydroxymethyl)methylglycine, 5-30 micrograms / mL of protease K and 1-50 mmol / L of saccharose, and a solvent is sterile water. The saliva preservingfluid can make a DNA sample preserved in the saliva preserving fluid at the normal temperature for a long time maintain integrity through the rational combination of all the components in a specificconcentration range, and can be directly used for PCR amplification without DNA extraction; the saliva preserving fluid can be used for an NGS experiment, can adapt to complex multiple PCR experiments, and can meet the requirement long-fragment amplification.