7 results about "Streptomycin sulphate" patented technology

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The present application relates to the technical field of artificial feeding of small insects, in particular to rubberwood small insects rubber tree wood chip cultivation feed and artificial feeding method. The rubberwood small insect cultivation feed comprises yeast powder, weiss salt, casein, starch, sucrose, agar powder, streptomycin sulfate, wheat germ oil, anhydrous ethanol, rubber tree wood chip and deionized water. The rubberwood small insect feeding method comprises the following steps: obtaining rubberwood small insect female adults; inoculating rubberwood small insect female adults; and feeding rubberwood small insects in a climate box. The artificial feeding method of rubberwood small insects provided by the present application can obtain stable test insect sources, provide insect source guarantee for studying the biological and ecological characteristics of rubberwood small insects, and further lay a theoretical foundation for formulating rubberwood small insect prevention and control measures.

The invention belongs to the technical field of medicines, and relates to application of an indole-3-aryl ketone derivative to preparation of a medicine for treating or preventing infectious diseases caused by staphylococcus aureus, in particular to application of the indole-3-aryl ketone derivative to preparation of a medicine for treating or preventing infectious diseases caused by drug-resistant staphylococcus aureus. The chemical structural formula of the indole-3-aryl ketone derivative disclosed by the invention is as shown in a formula I shown in the specification. Results of the invention show that the compound I (namely the indole-3-aryl ketone derivative as shown in the formula I) has strong inhibitory activity on methicillin-resistant staphylococcus aureus ATCC43300, the inhibitory effect is dependent on concentration gradient, the MIC50 value is 27.45 mu g / mL, and the MIC50 value of a positive drug streptomycin sulfate is greater than 200 mu g / mL. Therefore, the indole-3-aryl ketone derivative can be used for treating or preventing infectious diseases caused by the staphylococcus aureus.

The invention relates to the technical field of blood smear teaching, and particularly discloses a simulated liquid for blood smear preparation teaching, which comprises the following components by mass volume concentration: pure milk as a basic solute, 20.0 g / L of allura red, 2.0 g / L of sodium carboxymethyl cellulose, 2.0 g / L of potassium sorbate, 0.10 g / L of streptomycin sulfate for injection, and water-based brilliant blue essence, the finished product of the formula creates a safe and harmless teaching environment for teachers and students, is wide in raw material purchasing channel and low in cost, effectively reduces the teaching cost, can flexibly adjust the production batch and the yield according to the teaching scale, is not limited by the number of volunteers, blood station inventory and other factors, and is suitable for popularization and application. Students can take simulated blood anytime and anywhere to do infinite repeated exercises, the skill level of grassroots prevention and treatment personnel and medical examination students can be effectively improved, and popularization and promotion of related technologies are assisted.

The invention belongs to the field of insect feeding and insect resistance identification, and particularly relates to an inter-field insect medium for armyworms and / or grassland spodoptera exigua and application. The invention provides an inter-field insect medium for armyworms and / or grassland spodoptera exigua, which comprises puffed corn flour, puffed soybean flour, corn leaf meal, wheat germ, beer yeast, milk powder, wheat bran, casein, ascorbic acid, multivitamin glucose, erythromycin, sodium sorbate, methyl p-hydroxybenzoate, propyl p-hydroxybenzoate, cholesterol, inositol and streptomycin sulfate. Vermiculite and water. The raw materials of the field insect inoculation medium are easy to obtain, the cost is low, preparation is easy, storage is convenient, the field large-scale artificial insect inoculation efficiency of armyworms and spodoptera frugiperda can be improved, the influence of natural factors on the insect inoculation effect is reduced, meanwhile, the stability and repeatability of the insect inoculation effect are guaranteed, and optimization of the economic cost is achieved.

The application provides a preparation method of 30S ribosomal protein S1 and use of the 30S ribosomal protein S1 as an antibacterial synergist, and belongs to the technical field of biological medicines. The 30S ribosomal protein S1 in the application is a bioactive protein produced by Acinetobacter bacteria, and has the effect of enhancing the antibacterial capacity of antibiotics. In the process of being combined with streptomycin sulfate, the 30S ribosomal protein S1 greatly enhances the inhibiting capacity of streptomycin sulfate on avian pathogenic E.coli without showing the effect of inhibiting bacteria itself, which lays a foundation for future practical application, and also plays a reference role for the development of new antibacterial synergists.