Artificial feed for spodoptera frugiperda as well as preparation method and feeding method of spodoptera frugiperda

Abstract

The invention belongs to the technical field of insect feeding, and particularly relates to artificial feed for spodoptera frugiperda. Comprising the following raw materials in parts by mass: 10 to 30 parts of soybean meal, 30 to 50 parts of wheat germ, 70 to 100 parts of wheat bran, 10 to 50 parts of corn flour, 20 to 50 parts of crushed corn leaves, 15 to 40 parts of beer yeast powder, 5 to 30 parts of five-vitamin glucose, 5 to 20 parts of casein, 5 to 15 parts of ascorbic acid, 25 to 35 parts of agar powder, 0.5 to 2.0 parts of choline, 1.0 to 3.0 parts of sorbic acid, 0.1 to 0.5 part of inositol, 0.05 to 0.20 part of streptomycin sulfate, 1.0 to 3.0 parts of methyl parahydroxybenzoate, 15 to 30 parts of konjaku flour, 15 to 35 parts of milk powder, 0.2 to 2.0 parts of vitamin B6 and 1.0 to 1.0 part of corn germ oil. 5.0 parts of water and 1000 to 1500 parts of water. The artificial feed can significantly shorten the developmental duration of spodoptera frugiperda and improve the pupation rate and eclosion rate.

Description

Technical Field

[0001] The present invention belongs to the technical field of insect breeding, and particularly relates to an artificial feed for Spodoptera frugiperda, a preparation method thereof, and a breeding method thereof. Background Art

[0002] Spodoptera frugiperda, also known as fall armyworm, belongs to Lepidoptera, Noctuidae, and Spodoptera. It is a major invasive pest, with characteristics such as strong reproductive ability, a wide variety of host species, large food intake of older larvae, and miscellaneous feeding habits. Spodoptera frugiperda has a wide host range. In addition to damaging common crops such as corn, sugarcane, and rice, it also includes 76 families and 353 species such as sorghum and wheat. Spodoptera frugiperda mainly feeds directly on plant leaves and causes damage by boring, resulting in a large reduction in yield or even crop failure of the damaged crops, causing huge economic losses to the crops.

[0003] This insect is native to the tropical and subtropical regions of the Americas and has strong migratory ability. Due to the influence of frequent international trade activities and monsoon climate, Spodoptera frugiperda invaded Africa from the Americas in 2016 and then rapidly spread in 44 countries south of the Sahara. In August 2018, the Food and Agriculture Organization of the United Nations issued a global warning and listed this pest as one of the world's top ten plant pests.

[0004] At present, Spodoptera frugiperda has invaded China. To further control the harm caused by the invasion of Spodoptera frugiperda to our country's crops and the huge economic losses it brings. At this stage, it is urgent to carry out comprehensive prevention and control. A large number of test insects of Spodoptera frugiperda are required for indoor biological and behavioral research, screening and determination of highly effective and low-toxic chemical pesticides and new biological pesticides, identification of insect resistance of transgenic corn, and variety breeding. Whether the indoor artificial breeding of Spodoptera frugiperda is successful has become an important restricting factor for studying the characteristics of Spodoptera frugiperda, influencing the development of highly effective and low-toxic chemical pesticides and new biological pesticides, and the progress of transgenic insect-resistant corn breeding. Therefore, mass-producing test insects of Spodoptera frugiperda with consistent development under indoor conditions is of great significance for carrying out research work related to Spodoptera frugiperda.

[0005] There have been reports on the artificial feed and artificial breeding method of Spodoptera frugiperda in China, but the growth and development period of Spodoptera frugiperda is relatively long, and the pupation rate and emergence rate are still relatively low. Therefore, it is particularly urgent to shorten the growth and development period of Spodoptera frugiperda and improve the pupation rate and emergence rate of Spodoptera frugiperda. Summary of the Invention

[0006] To solve the above problems, the present invention provides an artificial feed for Spodoptera frugiperda, a preparation method thereof, and a breeding method thereof. The artificial feed for Spodoptera frugiperda provided by the present invention can significantly shorten the growth and development period of Spodoptera frugiperda and improve the pupation rate and emergence rate of Spodoptera frugiperda.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The present invention provides an artificial diet for Spodoptera frugiperda, comprising the following raw materials in parts by mass: 10-30 parts of soybean powder, 30-50 parts of wheat germ, 70-100 parts of wheat bran, 10-50 parts of corn flour, 20-50 parts of crushed corn leaves, 15-40 parts of beer yeast powder, 5-30 parts of five-dimensional glucose, 5-20 parts of casein, 5-15 parts of ascorbic acid, 25-35 parts of agar powder, 0.5-2.0 parts of choline, 1.0-3.0 parts of sorbic acid, 0.1-0.5 parts of inositol, 0.05-0.20 parts of streptomycin sulfate, 1.0-3.0 parts of methyl p-hydroxybenzoate, 15-30 parts of konjac powder, 15-35 parts of milk powder, 0.2-2.0 parts of vitamin B6, 1.0-5.0 parts of corn germ oil, and 1000-1500 parts of water.

[0009] The present invention provides a preparation method of the artificial diet described in the above technical solution, wherein the raw materials are mixed to obtain the artificial diet.

[0010] Preferably, the mixing includes the following steps:

[0011] Mix soybean powder, wheat bran, corn flour and beer yeast powder to obtain a first component;

[0012] Mix agar powder, sorbic acid, methyl p-hydroxybenzoate and a first portion of water to obtain a second component;

[0013] Mix konjac powder with a second portion of water to obtain a third component;

[0014] Mix wheat germ, crushed corn leaves, milk powder and the first component to obtain a fourth component;

[0015] Mix five-dimensional glucose, casein and a third portion of water to obtain a seventh component;

[0016] Mix the seventh component with the fourth component and let it stand to obtain a fifth component;

[0017] Mix ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6 and corn germ oil with the second component to obtain a sixth component;

[0018] Mix the sixth component, the third component and the fifth component;

[0019] The mass of the first portion of water accounts for 40%-55% of the total mass of water; the mass of the second portion of water accounts for 15%-25% of the total mass of water; the mass of the third portion of water accounts for 30%-40% of the total mass of water.

[0020] Preferably, the soybean powder, wheat bran, corn flour, and brewer's yeast powder are sterilized separately and then mixed; or the soybean powder, wheat bran, corn flour, and brewer's yeast powder are mixed and then sterilized.

[0021] Preferably, the preparation method of the second component includes: mixing agar powder, sorbic acid, methyl p-hydroxybenzoate, and the first portion of water, and maintaining at 121 °C for 30 min to obtain the second component.

[0022] Preferably, after obtaining the second component, when the temperature of the second component cools to 50 - 60 °C, it is mixed with ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6, and corn germ oil to obtain the sixth component.

[0023] The present invention provides a method for artificially raising Spodoptera frugiperda, using the artificial feed described in the above technical solution to raise Spodoptera frugiperda.

[0024] Preferably, the method of artificial raising includes the following steps:

[0025] 1) The egg masses laid for 72 - 84 h are placed on the surface of the artificial feed described in the above technical solution for raising; the density of the raised insects is 100 - 120 heads / 100 cm 2 ;

[0026] 2) When the larvae enter the third instar stage, the larvae are raised individually using the artificial feed described in the above technical solution;

[0027] 3) Three days after pupation, the pupae are raised using the artificial feed described in the above technical solution; the density of the raised adults is 15 - 25 heads / 100 cm 3 ;

[0028] 4) After eclosion, double-layer gauze is laid on the top of the insect rearing cage to facilitate adult oviposition; cotton balls containing honey water are placed at the bottom of the insect rearing cage to facilitate adult nutrition supplementation.

[0029] Preferably, the raising conditions in step 1) are: temperature 25 - 27 °C, relative humidity 60% - 80%, light cycle (15 - 18)L:(6 - 9)D; the raising conditions in step 2) are: temperature 25 - 28 °C, relative humidity 60% - 70%, light cycle (15 - 18)L:(6 - 9)D.

[0030] Preferably, the raising conditions in step 3) are: temperature 27 - 29 °C, relative humidity 70% - 80%, light cycle (15 - 18)L:(6 - 9)D.

[0031] Beneficial effects:

[0032] The present invention provides an artificial diet for Spodoptera frugiperda, comprising the following raw materials in parts by mass: 10 - 30 parts of soybean powder, 30 - 50 parts of wheat germ, 70 - 100 parts of wheat bran, 10 - 50 parts of corn flour, 20 - 50 parts of crushed corn leaves, 15 - 40 parts of brewer's yeast powder, 5 - 30 parts of pentavitamin glucose, 5 - 20 parts of casein, 5 - 15 parts of ascorbic acid, 25 - 35 parts of agar powder, 0.5 - 2.0 parts of choline, 1.0 - 3.0 parts of sorbic acid, 0.1 - 0.5 parts of inositol, 0.05 - 0.20 parts of streptomycin sulfate, 1.0 - 3.0 parts of methyl p-hydroxybenzoate, 15 - 30 parts of konjac powder, 15 - 35 parts of milk powder, 0.2 - 2.0 parts of vitamin B6, 1.0 - 5.0 parts of corn germ oil, and 1000 - 1500 parts of water. The artificial diet provided by the present invention has a balanced proportion of various nutritional components, and by optimizing the types and ratios of antibiotics and vitamins, the pupation rate and emergence rate of Spodoptera frugiperda are greatly improved, and the developmental duration of Spodoptera frugiperda is shortened. Brief Description of the Drawings

[0033] Figure 1 is the artificial diet for Spodoptera frugiperda provided by the present invention;

[0034] Figure 2 is the egg laying amount of Spodoptera frugiperda after being fed with the artificial diet obtained in Example 1 in Application Example 1;

[0035] Figure 3 is the larval morphology of Spodoptera frugiperda after being fed with the artificial diet obtained in Example 1 in Application Example 1;

[0036] Figure 4 is the pupal morphology of Spodoptera frugiperda after being fed with the artificial diet obtained in Example 1 in Application Example 1;

[0037] Figure 5 is the adult morphology of Spodoptera frugiperda after being fed with the artificial diet obtained in Example 1 in Application Example 1. Detailed Embodiments

[0038] The present invention provides an artificial diet for Spodoptera frugiperda, comprising the following raw materials in parts by mass: 10 - 30 parts of soybean powder, 30 - 50 parts of wheat germ, 70 - 100 parts of wheat bran, 10 - 50 parts of corn flour, 20 - 50 parts of crushed corn leaves, 15 - 40 parts of brewer's yeast powder, 5 - 30 parts of pentavitamin glucose, 5 - 20 parts of casein, 5 - 15 parts of ascorbic acid, 25 - 35 parts of agar powder, 0.5 - 2.0 parts of choline, 1.0 - 3.0 parts of sorbic acid, 0.1 - 0.5 parts of inositol, 0.05 - 0.20 parts of streptomycin sulfate, 1.0 - 3.0 parts of methyl p-hydroxybenzoate, 15 - 30 parts of konjac powder, 15 - 35 parts of milk powder, 0.2 - 2.0 parts of vitamin B6, 1.0 - 5.0 parts of corn germ oil, and 1000 - 1500 parts of water.

[0039] In parts by mass, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 10 to 30 parts of soybean powder. As an implementation mode, the mass fraction of the soybean powder in the present invention is 15 to 25 parts; as another implementation mode, the mass fraction of the soybean powder in the present invention is 20 parts. The soybean powder supplements high-quality plant protein for the body and at the same time supplements rich calcium for the body. The present invention has no special requirements for the source of the soybean powder, and conventional commercially available products in the art can be used.

[0040] Based on the mass fraction of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 15 to 40 parts of beer yeast powder. As an implementation mode, the mass fraction of the beer yeast powder in the present invention is 20 to 30 parts; as another implementation mode, the mass fraction of the beer yeast powder in the present invention is 26 parts. The beer yeast powder can stabilize the blood sugar of the Spodoptera frugiperda body while supplementing dietary fiber, which helps to maintain metabolism. The present invention has no special requirements for the source of the beer yeast powder, and conventional commercially available products in the art can be used.

[0041] Based on the mass fraction of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 15 to 35 parts of milk powder. As an implementation mode, the mass fraction of the milk powder in the present invention is 18 to 30 parts; as another implementation mode, the mass fraction of the milk powder in the present invention is 20 parts. The milk powder can supplement the minerals and vitamins required by the insect body, balance the nutrition, and avoid malnutrition caused by unbalanced nutrition of the insect body. The present invention has no special requirements for the source of the milk powder, and conventional commercially available products in the art can be used.

[0042] The soybean powder, beer yeast and milk powder of the present invention are all rich in protein. The three are compounded to provide balanced nutrition in many aspects, maintain a healthy metabolic level, provide the necessary nitrogen source for the growth of Spodoptera frugiperda, and can promote the growth and development of the insect body and improve the vitality of the insect body.

[0043] Based on the mass fraction of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 10 to 50 parts of corn flour. As an implementation mode, the mass fraction of the corn flour in the present invention is 15 to 30 parts; as another implementation mode, the mass fraction of the corn flour in the present invention is 20 parts. The present invention has no special requirements for the source of the corn flour, and conventional commercially available products in the art can be used. The corn flour mainly provides the required carbon source for Spodoptera frugiperda for normal growth. At the same time, it can also promote the insect body to be willing to actively ingest the feed.

[0044] Based on the parts by mass of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 5 to 30 parts of pentavitin. As an embodiment, the parts by mass of the pentavitin in the present invention are 15 to 20 parts; as another embodiment, the parts by mass of the pentavitin in the present invention are 10 parts. Pentavitin contains various vitamins and glucose, which not only provides the required carbon source for Spodoptera frugiperda, but also supplements the nutrients required by Spodoptera frugiperda, promotes the growth of Spodoptera frugiperda, and is beneficial to shortening the growth period, increasing the pupation rate and emergence rate. The present invention has no special requirements for the source of pentavitin, and conventional commercially available products in the art can be used.

[0045] Based on the parts by mass of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 0.2 to 2.0 parts of vitamin B6. As an embodiment, the parts by mass of the vitamin B6 in the present invention are 0.5 to 1.5 parts; as another embodiment, the parts by mass of the vitamin B6 in the present invention are 1 part. Vitamin B6 can promote the metabolism and synthesis of proteins in insects, contribute to the metabolism and decomposition of fats, and help maintain the growth and repair of insect tissues. The present invention has no special requirements for the source of vitamin B6, and conventional commercially available products in the art can be used.

[0046] The pentavitin and vitamin B6 of the present invention can provide vitamins that are necessary to maintain the normal physiological functions of the insect body and cannot be synthesized by the insect body itself and must be obtained through diet. Vitamins play an important role in the insect body, and a scientific and balanced vitamin ratio can promote insect metabolism, growth and development, and enhance insect immunity.

[0047] Based on the parts by mass of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 30 to 50 parts of wheat germ. As an embodiment, the parts by mass of the wheat germ in the present invention are 35 to 45 parts; as another embodiment, the parts by mass of the wheat germ in the present invention are 40 parts. Wheat germ provides various vitamins and trace elements for Spodoptera frugiperda, promotes the growth of Spodoptera frugiperda, and shortens the developmental duration. The present invention has no special requirements for the source of wheat germ, and conventional commercially available products in the art can be used.

[0048] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 70 to 100 parts of wheat bran. As an embodiment, the mass parts of the wheat bran in the present invention are 75 to 90 parts; as another embodiment, the mass parts of the wheat bran in the present invention are 80 parts. The wheat bran mainly provides the crude fiber required by Spodoptera frugiperda, promotes the digestion and absorption of the artificial feed by Spodoptera frugiperda, and is beneficial to the healthy growth of Spodoptera frugiperda. The present invention has no special requirements on the source of the wheat bran, and conventional commercially available products in the art can be used.

[0049] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 20 to 50 parts of crushed corn leaves. As an embodiment, the mass parts of the crushed corn leaves in the present invention are 75 to 90 parts; as another embodiment, the mass parts of the crushed corn leaves in the present invention are 30 parts. The crushed corn leaves mainly provide crude fiber. At the same time, the crushed corn leaves are very close to the food source of Spodoptera frugiperda in the field; they jointly promote the digestion and absorption of the artificial feed by Spodoptera frugiperda with the wheat bran, and are also beneficial for the insects to feed. The present invention has no special requirements on the source of the crushed corn leaves, and conventional commercially available products in the art can be used.

[0050] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 1 to 3 parts of methyl p-hydroxybenzoate. As an embodiment, the mass parts of the methyl p-hydroxybenzoate in the present invention are 1.5 to 2.5 parts; as another embodiment, the mass parts of the methyl p-hydroxybenzoate in the present invention are 2 parts. The present invention has no special requirements on the source of the methyl p-hydroxybenzoate, and conventional commercially available products in the art can be used.

[0051] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 1 to 3 parts of sorbic acid. As an embodiment, the mass parts of the sorbic acid in the present invention are 1.5 to 2.5 parts; as another embodiment, the mass parts of the sorbic acid in the present invention are 2 parts. The present invention has no special requirements on the source of the sorbic acid, and conventional commercially available products in the art can be used.

[0052] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 0.05 to 0.2 parts of streptomycin sulfate. As an embodiment, the mass parts of the streptomycin sulfate in the present invention are 0.075 to 0.15 parts; as another embodiment, the mass parts of the streptomycin sulfate in the present invention are 0.1 part. The present invention has no special requirements on the source of the streptomycin sulfate, and conventional commercially available products in the art can be used.

[0053] Methyl paraben, sorbic acid and streptomycin sulfate are the main antibacterial substances in the present invention. Methyl paraben belongs to a bactericidal preservative, which is effective against various molds, yeasts and bacteria, and the bactericidal power of nipagin ester is low; sorbic acid has an inhibitory effect on yeasts, molds and many fungi; streptomycin sulfate mainly has good antibacterial activity against Gram-negative bacteria. The present invention mixes and uses these three components, which can expand the antibacterial spectrum. The three components have good additivity and synergy, significantly improving the antibacterial and bacteriostatic effects, increasing the pupation rate and emergence rate of Spodoptera frugiperda, and reducing the occurrence of diseases of Spodoptera frugiperda.

[0054] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 5-20 parts of casein. As an embodiment, the mass parts of the casein in the present invention are 6-10 parts; as another embodiment, the mass parts of the casein in the present invention are 8 parts. Casein is mainly used to supplement the casein of the insect body and promote the growth of the insect body. The present invention has no special requirements for the source of casein, and conventional commercially available products in the art can be used.

[0055] By mass, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 5-15 parts of ascorbic acid; more preferably 6-10 parts; even more preferably 8 parts. Ascorbic acid can provide vitamin C for the insect body, and at the same time can prevent scurvy and enhance the immunity of the insect body. The present invention has no special requirements for the source of ascorbic acid, and conventional commercially available products in the art can be used.

[0056] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 0.5-2 parts of choline. As an embodiment, the mass parts of the choline in the present invention are 0.75-1.5 parts; as another embodiment, the mass parts of the choline in the present invention are 1 part. Choline is an important component of biological membranes. Without it, biological membranes cannot be effectively synthesized, inhibiting the growth of the insect body. The present invention has no special requirements for the source of choline, and conventional commercially available products in the art can be used.

[0057] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 0.1-0.5 parts of inositol. As an embodiment, the mass parts of the inositol in the present invention are 0.15-0.3 parts; as another embodiment, the mass parts of the inositol in the present invention are 0.2 parts. Inositol can promote cell metabolism, contribute to development, increase the appetite of the insect body, reduce cholesterol, and help the redistribution of body fat. The present invention applies inositol and choline simultaneously, and the effect of promoting the growth of the insect body is better. The present invention has no special requirements for the source of inositol, and conventional commercially available products in the art can be used.

[0058] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 15-30 parts of konjac powder. As an embodiment, the mass parts of the konjac powder in the present invention are 17-25 parts; as another embodiment, the mass parts of the konjac powder in the present invention are 20 parts. Konjac powder can mainly supplement the dietary fiber required for the growth and development of the insect body. The present invention has no special requirements for the source of konjac powder, and conventional commercially available products in the art can be used.

[0059] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 1-5 parts of corn germ oil. As an embodiment, the mass parts of the corn germ oil in the present invention are 1.5-3 parts; as another embodiment, the mass parts of the corn germ oil in the present invention are 2 parts. Corn germ oil can supplement vitamin E and unsaturated fatty acids required for the growth and development of the insect body, and promote the growth of the insect body. The present invention has no special requirements for the source of corn germ oil, and conventional commercially available products in the art can be used.

[0060] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 25-35 parts of agar powder. As an embodiment, the mass parts of the agar powder in the present invention are 27-33 parts; as another embodiment, the mass parts of the agar powder in the present invention are 30 parts. Agar powder is a coagulant for forming. The present invention has no special requirements for the source of agar powder, and conventional commercially available products in the art can be used.

[0061] Based on the mass parts of the soybean powder, the raw materials of the artificial feed for Spodoptera frugiperda provided by the present invention include 1000-1500 parts of water. As an embodiment, the mass parts of the water in the present invention are 1100-1300 parts; as another embodiment, the mass parts of the water in the present invention are 1200 parts. The present invention has no special requirements for the type of water, and conventionally sterilized and filtered water can be used.

[0062] The proportion of each nutritional component of the artificial feed for Spodoptera frugiperda provided by the present invention is balanced. By optimizing the types and ratios of antibiotics and vitamins, while the feed does not grow miscellaneous bacteria and Spodoptera frugiperda grows and develops normally, the pupation rate and emergence rate of Spodoptera frugiperda are greatly improved, and the development period of Spodoptera frugiperda is shortened; moreover, the raw materials of the artificial feed of the present invention are few in type, low in cost, easy to obtain, and convenient to prepare.

[0063] The present invention also provides a preparation method of the artificial feed described in the above technical solution, mixing the raw materials to obtain the artificial feed.

[0064] In the present invention, the mixing preferably comprises the following steps: mixing soybean powder, wheat bran, corn flour and beer yeast powder to obtain a first component; mixing agar powder, sorbic acid, methyl p-hydroxybenzoate and a first portion of water to obtain a second component; mixing konjac powder with a second portion of water to obtain a third component; mixing wheat germ, crushed corn leaves, milk powder and the first component to obtain a fourth component; mixing pentaose glucose, casein and a third portion of water to obtain a seventh component; mixing the seventh component with the fourth component and standing to obtain a fifth component; mixing ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6 and corn germ oil with the second component to obtain a sixth component; mixing the sixth component, the third component and the fifth component; the mass of the first portion of water accounts for 40% - 55% of the total mass of water; the mass of the second portion of water accounts for 15% - 25% of the total mass of water; the mass of the third portion of water accounts for 30% - 40% of the total mass of water.

[0065] In the present invention, it is preferred to mix soybean powder, wheat bran, corn flour and beer yeast powder to obtain a first component.

[0066] In the present invention, the soybean powder, wheat bran, corn flour and beer yeast powder are preferably sterilized separately and then mixed; or the soybean powder, wheat bran, corn flour and beer yeast powder are preferably mixed and then sterilized. The present invention has no special requirements for the sterilization method, and the conventional sterilization methods in the art can be used. In the embodiments of the present invention, the sterilization method is preferably high-temperature and high-pressure sterilization; the conditions of the high-temperature and high-pressure sterilization are preferably sterilized at 121 °C for 30 min. The present invention has no special requirements for the mixing method, and the conventional mixing methods in the art can be used to mix evenly.

[0067] In the present invention, it is preferred to mix agar powder, sorbic acid, methyl p-hydroxybenzoate and a first portion of water to obtain a second component.

[0068] In the present invention, the preparation method of the second component comprises: mixing agar powder, sorbic acid, methyl p-hydroxybenzoate and a first portion of water, and maintaining at 121 °C for 30 min to obtain the second component. Since the solubility of sorbitol and methyl p-hydroxybenzoate in normal-temperature water is very low, the present invention makes sorbitol and methyl p-hydroxybenzoate fully dissolve and mix evenly by high-temperature heating; heating agar together with sorbitol and methyl p-hydroxybenzoate can further simplify the operation steps and at the same time sterilize the second component. The mass of the first portion of water in the present invention preferably accounts for 40% - 55% of the total mass of water; further preferably 45% - 52%; still further preferably 50%. The first portion of water in the present invention not only ensures the solubility and operability of the second component, but also ensures the molding of the overall feed after mixing with other components.

[0069] After obtaining the second component, the present invention preferably waits until the temperature of the second component cools down and then mixes it with ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6, and corn germ oil to obtain the sixth component. In the present invention, waiting for the temperature of the second component to cool down before mixing it with ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6, and corn germ oil can prevent the inactivation of active ingredients such as ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6, and corn germ oil, and reduce the feeding effect of artificial feed.

[0070] In the present invention, the temperature at which the second component is cooled is preferably 50°C to 60°C; more preferably 55°C to 60°C; even more preferably 60°C.

[0071] The present invention preferably mixes konjac flour with a second portion of water to obtain a third component.

[0072] In the present invention, the mass of the second portion of water preferably accounts for 15% to 25% of the total mass of water; more preferably 15% to 20%; even more preferably 16.7%. The second portion of water in the present invention not only ensures the solubility and operability of the third component but also ensures the formation of the overall feed after mixing with other components. The present invention has no special requirements for the method of mixing konjac flour with the second portion of water, and it can be uniformly mixed by using a conventional mixing method in the art. The third component of the present invention is preferably sterilized before use. The present invention has no special requirements for the sterilization method, and a conventional sterilization method in the art can be used. In the examples of the present invention, the sterilization method of the third component is preferably high-temperature and high-pressure sterilization; the conditions of the high-temperature and high-pressure sterilization are preferably sterilization for 30 minutes at 121°C.

[0073] The present invention preferably mixes wheat germ, crushed corn leaves, milk powder, and the first component to obtain a fourth component. The present invention has no special requirements for the mixing method, and it can be uniformly mixed by using a conventional mixing method in the art. The present invention does not need to sterilize the raw materials for preparing the fourth component. Not sterilizing wheat germ and crushed corn leaves can better conform to the feeding tendency and nutritional components of insects in the field.

[0074] The present invention preferably mixes pentavitamin glucose, casein, and a third portion of water to obtain a seventh component.

[0075] In the present invention, the mass of the third portion of water preferably accounts for 30% to 40% of the total mass of water; more preferably 30% to 35%; even more preferably 33.3%. The present invention has no special requirements for the mixing method, and it can be uniformly mixed by using a conventional mixing method in the art.

[0076] After obtaining the seventh component and the fourth component, the present invention preferably mixes the seventh component and the fourth component and allows them to stand to obtain a fifth component. The present invention has no special requirements for the mixing method, and any conventional mixing method in the art can be used for uniform mixing. The present invention preferably allows the fifth component to stand before use. The standing time in the present invention is preferably 1.5 to 3 h; more preferably 1.5 to 2.5 h; even more preferably 2 h. Standing in the present invention is beneficial for different components to fully absorb water and for the components to be uniformly mixed; at the same time, sufficient standing is beneficial for the action of Saccharomyces cerevisiae.

[0077] After obtaining the sixth component, the third component and the fifth component, the present invention preferably mixes the sixth component, the third component and the fifth component to obtain the artificial feed of the present invention. The present invention has no special requirements for the mixing method, and any conventional mixing method in the art can be used for uniform mixing.

[0078] The preparation method of the artificial feed for Spodoptera frugiperda provided by the present invention has a simple preparation process, less time consumption and is convenient for popularization.

[0079] The present invention also provides an artificial feeding method for Spodoptera frugiperda, using the artificial feed described in the above technical solution to feed Spodoptera frugiperda.

[0080] The artificial feeding method of the present invention preferably includes the following steps:

[0081] 1) Connect the egg masses laid for 72 - 84 h to the surface of the artificial feed described in the above technical solution for feeding; the feeding insect density is 100 - 120 heads / 100 cm 2 ;

[0082] 2) When the larvae enter the third instar stage, feed the larvae individually with the artificial feed described in the above technical solution;

[0083] 3) Three days after pupation, feed the pupae with the artificial feed described in the above technical solution; the feeding adult density is 15 - 25 heads / 100 cm 2 ;

[0084] 4) After eclosion, lay a double layer of gauze on the top of the insect rearing cage to facilitate adult oviposition; place a cotton ball containing honey water at the bottom of the insect rearing cage to facilitate adult nutrition supplementation.

[0085] The egg masses in the present invention are preferably collected by the following method: Remove the gauze with Spodoptera frugiperda egg masses from the upper part of the insect rearing cage, cut the gauze, and each piece of gauze preferably carries 2 - 3 egg masses; place the cut gauze in a self-sealing bag with a moistened cotton ball at the bottom for cultivation.

[0086] In the present invention, the culture temperature of the egg mass is preferably 25 to 27 °C, more preferably 26 °C. The relative humidity for culturing the egg mass of the present invention is preferably 50% to 70%; more preferably 55% to 65%; even more preferably 60%. The light cycle for culturing the egg mass of the present invention is preferably (15 to 18)L:(6 to 9)D, more preferably (16 to 17)L:(7 to 8)D, even more preferably 16L:8D. The culture conditions of the egg mass of the present invention result in the highest hatching rate of the egg mass.

[0087] The present invention preferably places the egg mass 72 to 84 h after oviposition on the surface of the artificial feed described in the above technical solution for feeding.

[0088] In the present invention, the insect density for feeding is preferably 100 to 120 heads / 100 cm 2 ; more preferably 105 to 115 heads / 100 cm 2 ; even more preferably 110 heads / 100 cm 2 。The insect density of the present invention can not only ensure the living space but also meet the food source requirements. The feeding temperature of the present invention is preferably 25 to 27 °C, more preferably 26 °C. The relative humidity for feeding of the present invention is preferably 60% to 80%, more preferably 60% to 70%, even more preferably 60%. The light cycle for feeding of the present invention is preferably (15 to 18)L:(6 to 9)D, more preferably (16 to 17)L:(7 to 8)D, even more preferably 16L:8D. The present invention preferably places the egg mass in a feeding box for feeding. The dimensions of the length, width and height of the feeding box of the present invention are preferably (25 to 35) cm × (20 to 25) cm × (10 to 15) cm, more preferably 30 cm × 22 cm × 12 cm. The present invention preferably places the insect rearing box in an incubator for feeding to facilitate the control of feeding conditions.

[0089] After the larvae enter the third instar, the present invention preferably feeds the larvae individually with the artificial feed described in the above technical solution. The present invention preferably transfers the third instar larvae to an insect rearing box for individual feeding until pupation to prevent the larvae from cannibalizing each other.

[0090] In the present invention, the dimensions of the upper diameter, lower diameter and height of the insect rearing box are respectively preferably (6.0 to 8.0) cm, (5.0 to 7.0) cm and (3.0 to 5.0) cm, more preferably 7.3 cm, 5.8 cm and 3.5 cm. The insect rearing box of the present invention preferably has ventilation holes.

[0091] In the present invention, the feeding amount of the artificial feed is preferably ≥10 g / head, more preferably 20 - 10 g / head, and even more preferably 10 g / head. The temperature for the feeding in the present invention is preferably 25 - 28 °C, more preferably 26 °C. The relative humidity for the feeding in the present invention is preferably 60% - 70%, more preferably 60% - 65%, and even more preferably 60%. The light cycle for the feeding in the present invention is preferably (15 - 18)L:(6 - 9)D, more preferably (16 - 17)L:(7 - 8)D, and even more preferably 16L:8D.

[0092] 3 days after pupation, in the present invention, the pupae are preferably fed with the artificial feed described in the above technical solution.

[0093] In the present invention, the insect density for the feeding is preferably 15 - 25 heads / 100 cm 2 ; even more preferably 20 heads / 100 cm 2 . In the present invention, the pupae are preferably picked into a culture dish for culturing; the insect density in the culture dish is 180 - 210 heads / dish; more preferably 200 heads / dish. The feeding amount of the artificial feed in the present invention is preferably ≥10 g / head, more preferably 20 - 10 g / head, and even more preferably 10 g / head. In the present invention, the culture dish is preferably placed in an insect rearing cage for feeding. The dimensions of the length, width, and height of the insect rearing cage in the present invention are preferably (35 - 45) cm × (20 - 30) cm × (20 - 30) cm, more preferably 39 cm × 26 cm × 21 cm. The temperature for the feeding in the present invention is preferably 27 - 29 °C, more preferably 28 °C. In the present invention, the relative humidity for the feeding is preferably 70 - 80%, more preferably 75 - 80%, and even more preferably 80%. In the present invention, the light cycle for the feeding is preferably (15 - 18)L:(6 - 9)D, more preferably (16 - 17)L:(7 - 8)D, and even more preferably 16L:8D. The feeding conditions in the present invention are more conducive to the growth of the insect body.

[0094] After eclosion, in the present invention, a double - layer gauze is preferably laid on the top of the insect rearing cage to facilitate the adult insects to lay eggs; a cotton ball containing honey water is placed at the bottom of the insect rearing cage to facilitate the adult insects to supplement nutrition.

[0095] In the present invention, the mass percentage content of the honey water is preferably 2% - 15%; more preferably 8% - 12%; and even more preferably 10%.

[0096] The artificial rearing method of Spodoptera frugiperda provided by the present invention can significantly shorten the growth period of Spodoptera frugiperda, improve the pupation rate and eclosion rate of Spodoptera frugiperda; and the operation is simple, without any professional technical background, and can be mastered after simple training, being simple and labor - saving.

[0097] To further illustrate the present invention, a kind of artificial feed for Spodoptera frugiperda, its preparation method and feeding method provided by the present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0098] Example 1

[0099] An artificial feed for Spodoptera frugiperda is composed of the following raw materials in parts by mass:

[0100] 20 parts of soybean powder, 40 parts of wheat germ, 80 parts of wheat bran, 20 parts of corn flour, 30 parts of crushed corn leaves, 26 parts of brewer's yeast powder, 10 parts of pentavitamin glucose, 8 parts of casein, 8 parts of ascorbic acid, 30 parts of agar powder, 1 part of choline, 2 parts of sorbic acid, 0.2 part of inositol, 0.1 part of streptomycin sulfate, 2 parts of methyl p-hydroxybenzoate, 20 parts of konjac powder, 20 parts of milk powder, 2 parts of corn germ oil, 1 part of vitamin B6 and 1200 parts of water.

[0101] The specific preparation method consists of the following steps:

[0102] (1) Sterilize 20 g of soybean powder, 80 g of wheat bran, 20 g of corn flour and 26 g of brewer's yeast powder at 121 °C for 30 min to obtain the first component;

[0103] (2) Place 30 g of agar powder, 2 g of sorbic acid and 2 g of methyl p-hydroxybenzoate in 600 ml of water, and sterilize at 121 °C for 30 min to obtain the second component;

[0104] (3) Dissolve 20 g of konjac powder in 200 ml of water, and sterilize at 121 °C for 30 min to obtain the third component;

[0105] (4) Mix 40 g of wheat germ, 30 g of crushed corn leaves, 20 g of milk powder and the first component in step (1) thoroughly to obtain the fourth component;

[0106] (5) Dissolve 10 g of pentavitamin glucose and 8 g of casein in 400 ml of sterile water to obtain the seventh component;

[0107] (6) Stir and mix the seventh component in step (5) and the fourth component in step (4) thoroughly, and let it stand for 2 h to obtain the fifth component;

[0108] (7) When the second component in step (2) cools to 60 °C, add 8 g of ascorbic acid, 1 g of choline, 0.2 g of inositol, 0.1 g of streptomycin sulfate, 1 g of vitamin B6 and 2 g of corn germ oil to obtain the sixth component;

[0109] (8) Pour the sixth component in step (7) and the third component in step (3) into the fifth component in step (6), and stir well to obtain the artificial feed for Spodoptera frugiperda of the present invention. Figure 1 )

[0110] Example 2

[0111] An artificial feed for Spodoptera frugiperda is composed of the following raw materials in parts by mass:

[0112] Soybean powder 15 parts, wheat germ 45 parts, wheat bran 85 parts, corn flour 25 parts, crushed corn leaves 35 parts, brewer's yeast powder 25 parts, pentavitamin glucose 10 parts, casein 9 parts, ascorbic acid 9 parts, agar powder 32 parts, choline 1.5 parts, sorbic acid 2 parts, inositol 0.3 part, streptomycin sulfate 0.1 part, methyl p-hydroxybenzoate 2 parts, konjac powder 22 parts, milk powder 22 parts, corn germ oil 2 parts, vitamin B6 1.3 parts, and water 1300 parts.

[0113] The preparation method is the same as that of Example 1.

[0114] Example 3

[0115] An artificial feed for Spodoptera frugiperda is composed of the following raw materials in parts by mass:

[0116] Soybean powder 18 parts, wheat germ 43 parts, wheat bran 75 parts, corn flour 22 parts, crushed corn leaves 30 parts, brewer's yeast powder 23 parts, pentavitamin glucose 9 parts, casein 8 parts, ascorbic acid 9 parts, agar powder 30 parts, choline 1.1 parts, sorbic acid 1.8 parts, inositol 0.2 part, streptomycin sulfate 0.1 part, methyl p-hydroxybenzoate 1.8 parts, konjac powder 18 parts, milk powder 25 parts, corn germ oil 1.8 parts, vitamin B6 0.6 part, and water 1100 parts.

[0117] The preparation method is the same as that of Example 1.

[0118] Example 4

[0119] An artificial feed for Spodoptera frugiperda is composed of the following raw materials in parts by mass:

[0120] Soybean powder 16 parts, wheat germ 45 parts, wheat bran 85 parts, corn flour 15 parts, crushed corn leaves 30 parts, brewer's yeast powder 28 parts, pentavitamin glucose 10 parts, casein 8 parts, ascorbic acid 8 parts, agar powder 30 parts, choline 1.0 part, sorbic acid 2 parts, inositol 0.2 part, streptomycin sulfate 0.1 part, methyl p-hydroxybenzoate 2 parts, konjac powder 22 parts, milk powder 23 parts, corn germ oil 2.0 parts, vitamin B6 0.8 part, and water 1200 parts.

[0121] The preparation method is the same as that of Example 1.

[0122] Comparative Example 1

[0123] An artificial diet for Spodoptera frugiperda, with the raw material composition being the same as that in Example 1, except that methyl p - hydroxybenzoate is omitted.

[0124] The preparation method is the same as that in Example 1.

[0125] Comparative Example 2

[0126] An artificial diet for Spodoptera frugiperda, with the raw material composition being the same as that in Example 1, except that sorbic acid is omitted.

[0127] The preparation method is the same as that in Example 1.

[0128] Comparative Example 3

[0129] An artificial diet for Spodoptera frugiperda, with the raw material composition being the same as that in Example 1, except that penta - dimensional glucose is replaced by glucose.

[0130] The preparation method is the same as that in Example 1.

[0131] Comparative Example 4

[0132] An artificial diet for Spodoptera frugiperda, with the raw material composition being the same as that in Example 1, except that konjac powder is omitted.

[0133] The preparation method is the same as that in Example 1.

[0134] Comparative Example 5

[0135] Prepare the artificial diet for Spodoptera frugiperda according to the scheme disclosed in Patent CN109965088A.

[0136] Comparative Example 6

[0137] Prepare the artificial diet for Spodoptera frugiperda according to the scheme disclosed in Patent CN110583914A.

[0138] Application Example 1

[0139] Test insect source: The test Spodoptera frugiperda population is from the National Pilot and Industrialization Base for Genetically Modified Corn and Soybeans (Gongzhuling) and the Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (Northeast Innovation Center for Agricultural Science and Technology, China).

[0140] Use the artificial diets provided in Examples 1 - 4 and Comparative Examples 1 - 6 to feed Spodoptera frugiperda respectively. The specific feeding method consists of the following steps:

[0141] (1) Egg mass collection: Remove the gauze containing the fall armyworm egg mass from the top of the insect cage and cut the gauze. Each piece of gauze should preferably contain 2 to 3 eggs. Place the cut gauze in a ziplock bag with a moistened cotton ball at the bottom. Place the bag in an incubator at a temperature of (26±1)℃, a relative humidity of 60%, and a photoperiod of 16L:8D.

[0142] (2) Rearing of 1st to 2nd instar larvae: When the eggs are about to hatch, place the yarn carrying the eggs in a feeding box (length × width × height = 30 cm × 22 cm × 12 cm) filled with the above-mentioned artificial feed. The eggs are placed on the surface of the artificial feed. The insect density is 1000 / 100 cm. 2 The breeding box was placed in an incubator with a temperature of (26±1)°C, a relative humidity of 60%, and a photoperiod of 16h:8h (L:D) for breeding.

[0143] (3) Rearing of 3rd to 6th instar larvae and pre-pupae: When the larvae enter the 3rd instar, they are transferred to PP plastic insect boxes (upper diameter 7.3 cm, lower diameter 5.8 cm, height 3.5 cm, lid with air holes) and reared individually until pupation. The feeding amount of artificial diet for 3rd to 6th instar larvae is 10 g / head. The rearing conditions are: temperature (26 ± 1) °C, relative humidity 60%, and photoperiod of 16h:8h (L:D).

[0144] (4) Collection and feeding of pupae: After the color of fall armyworm pupae turns darker (about 3 days after pupation), pick the pupae into culture dishes, with about 200 pupae per dish. Place the culture dishes in insect cages (length × width × height = 39 cm × 26 cm × 21 cm) for feeding. The amount of artificial feed fed during the pupal stage is 10 g / head. The feeding conditions are: temperature is (28 ± 1) ° C, relative humidity is 80%, and the photoperiod is 16h:8h (L:D).

[0145] (5) Rearing of adults: After the pupae emerge from the insect cage, place a double layer of gauze on the top of the cage to facilitate egg laying by the adults. After the adults lay eggs, replace the gauze every day. At the same time, place several cotton balls containing 10% honey water at the bottom of the cage and replace them every day to facilitate nutrition for the adults.

[0146] During the feeding process according to the above method, the survival rate, insect weight (fed to the 13th day), pupa weight, pupation rate, eclosion rate, pupation time and eclosion time were observed and recorded. The survey data were sorted using EXCEL2007, and the data were analyzed for significant differences using SPSS23 statistical software. The results are shown in Table 1. The actual pictures of the egg laying amount, larval morphology, pupal morphology and adult morphology of the fall armyworm after feeding with the artificial diet in Example 1 are shown in Table 1. Figures 2 to 5 shown.

[0147] Table 1 Growth and development parameters of Spodoptera frugiperda fed with different formulated feeds

[0148]

[0149]

[0150] Note: Different lowercase letters in the same column of the table indicate significant differences between different treatments (P<0.05).

[0151] According to Table 1 and Figures 2 to 5 it can be seen that the artificial feed provided by the present invention can significantly shorten the development duration of Spodoptera frugiperda and improve the pupation rate and emergence rate.

[0152] The results of the above embodiments show that the artificial feed for Spodoptera frugiperda provided by the present invention can greatly improve the pupation rate and emergence rate of Spodoptera frugiperda and shorten the development duration of Spodoptera frugiperda.

[0153] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An artificial feed for Spodoptera frugiperda, characterized in that, It comprises the following raw materials in parts by mass: 10 - 30 parts of soybean powder, 30 - 50 parts of wheat germ, 70 - 100 parts of wheat bran, 10 - 50 parts of corn flour, 20 - 50 parts of crushed corn leaves, 15 - 40 parts of brewer's yeast powder, 5 - 30 parts of pentavitamin glucose, 5 - 20 parts of casein, 5 - 15 parts of ascorbic acid, 25 - 35 parts of agar powder, 0.5 - 2.0 parts of choline, 1.0 - 3.0 parts of sorbic acid, 0.1 - 0.5 parts of inositol, 0.05 - 0.20 parts of streptomycin sulfate, 1.0 - 3.0 parts of methyl p-hydroxybenzoate, 15 - 30 parts of konjac powder, 15 - 35 parts of milk powder, 0.2 - 2.0 parts of vitamin B6, 1.0 - 5.0 parts of corn germ oil, and 1000 - 1500 parts of water.

2. The preparation method of the artificial feed according to claim 1, characterized in that, Mix the above raw materials to obtain the artificial feed.

3. The preparation method according to claim 2, characterized in that, The mixing includes the following steps: Mix soybean powder, wheat bran, corn flour and brewer's yeast powder to obtain the first component; Mix agar powder, sorbic acid, methyl p-hydroxybenzoate and the first portion of water to obtain the second component; Mix konjac powder with the second portion of water to obtain the third component; Mix wheat germ, crushed corn leaves, milk powder and the first component to obtain the fourth component; Mix pentavitamin glucose, casein and the third portion of water to obtain the seventh component; Mix the seventh component with the fourth component and let it stand to obtain the fifth component; Mix ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6 and corn germ oil with the second component to obtain the sixth component; Mix the sixth component, the third component and the fifth component; The mass of the first portion of water accounts for 40% - 55% of the total mass of water; the mass of the second portion of water accounts for 15% - 25% of the total mass of water; the mass of the third portion of water accounts for 30% - 40% of the total mass of water.

4. The preparation method according to claim 3, characterized in that, The soybean powder, wheat bran, corn flour and brewer's yeast powder are sterilized separately and then mixed; or the soybean powder, wheat bran, corn flour and brewer's yeast powder are mixed and then sterilized.

5. The preparation method according to claim 3, characterized in that, The preparation method of the second component includes: mixing agar powder, sorbic acid, methyl p-hydroxybenzoate and the first portion of water, and maintaining at 121 °C for 30 min to obtain the second component.

6. The preparation method according to claim 5, characterized in that, After obtaining the second component, when the temperature of the second component cools to 50 - 60 °C, mix it with ascorbic acid, choline, inositol, streptomycin sulfate, vitamin B6 and corn germ oil to obtain the sixth component.

7. An artificial feeding method for Spodoptera frugiperda, characterized in that, Use the artificial feed according to claim 1 or the artificial feed obtained by the preparation method according to any one of claims 2 - 6 to feed Spodoptera frugiperda.

8. The artificial breeding method according to claim 7, characterized in that, The method for artificial feeding includes the following steps: 1) The egg masses laid 72 - 84 h ago are placed on the surface of the above-mentioned artificial feed for rearing; the density of the insects for rearing is 100 - 120 heads / 100 cm 2 ; 2) When the larvae enter the 3rd instar, feed the larvae individually with the above artificial feed; 3) After 3 days of pupation, raise the pupae with the artificial feed described above; the adult density during the raising is 15 - 25 individuals / 100 cm 3 ; 4) After eclosion, lay a double-layer gauze on the top of the insect rearing cage to facilitate adult oviposition; place cotton balls containing honey water at the bottom of the insect rearing cage to facilitate adult nutrition supplementation.

9. The artificial feeding method according to claim 8, characterized in that, The feeding conditions in step 1) are as follows: temperature 25 - 27 °C, relative humidity 60% - 80%, light cycle (15 - 18)L:(6 - 9)D; the feeding conditions in step 2) are as follows: temperature 25 - 28 °C, relative humidity 60% - 70%, light cycle (15 - 18)L:(6 - 9)D.

10. The artificial breeding method according to claim 8, characterized in that, The feeding conditions in step 3) are as follows: temperature 27 - 29 °C, relative humidity 70% - 80%, light cycle (15 - 18)L:(6 - 9)D.

Images