The invention provides a cultivation method of high-quality and high-yield white mushroom, comprising the following steps: domestication of white mushroom mother species, and cultivation of white mushroom, wherein the domestication of mother fungus is divided into domestication of three-level mother species; the cultivation of white mushroom includes: S1 Nutrient preparation, S2 cultivation management, and S3 stockpiling; wherein the nutrient includes the following raw materials in parts by weight: 50-72 parts of rubber sawdust, 44-58 parts of lychee sawdust, 45-68 parts of longan sawdust, 50-80 parts of broad-leaved wood chips, wheat bark 15 to 30 parts, 2 to 5 parts of gypsum, 3 to 8 parts of silkworm sand, 2 to 6 parts of bamboo charcoal powder, 5 to 12 parts of hibiscus bark, 4 to 11 parts of camphor wood powder, 5 to 15 parts of hematoxylin shavings, and fenxin wood 3 to 10 parts, 2 to 5 parts of sugar; the white mushroom of the invention has high yield, large mushroom body, thick and uniform mushroom flesh, high temperature resistance, high nutritional value, can effectively prevent insect damage, and increase yield.