The invention belongs to the field of bamboo treatment and relates to an antiseptic and mildewproof treatment technology. The technology comprises the following steps that (1), the fresh bamboo is dried to be with the water content of 12-15%; (2), the dried bamboo is put into a vacuum pressure tank, the vacuum degree of the vacuum pressure tank is controlled to be over 0.08 MPa, and the treatment time ranges from 35 min to 50 min; (3), a protective agent is added to the vacuum pressure tank; (4), a vacuum pump is closed, a pressure pump is started, the pressure is made to be in the range of 1.2-1.8 MPa, and treatment is conducted for 50-70 min under the condition of keeping the pressure; and (5), the pressure is relieved, and the protective agent is drained. According to the antiseptic and mildewproof treatment technology for the bamboo, for the characteristic of the bamboo, the technological processes of drying, high-pressure, vacuum, pressurization and vacuum are adopted, in combination with the protective agent special for the bamboo, the protective agent can be effectively permeated into the bamboo from bamboo inner rings (inner of the bamboo) with the high nutrient content and the low density, the ideal protective agent saturation injection effect is achieved, the protective agent stays in the bamboo for a long time and is not easy to drain, and the long-term protective effect can be played.