The invention discloses a preparation method of a tea dyed costume fabric. By the use of fatty alcohol-polyoxyethylene ether, sodium citrate, hydroxypropyl methylcellulose, ethyl maltol and 2-hydracrylic acid, permeation of water is accelerated, so that tea polyphenol can quickly enter water, and the extraction time is shortened; 2-phosphonic acid butane-1,2,4-tricarboxylic acid sodium salt, sodium tetraphenylboron, 1,2,3,4,5,6-cyclohexanehexacarboxylic acid, sorbitol, ethanediol di(2-aminoethyl ether)quadrol enhance tea even dyeing, so that the dyeing is uniform and consistent, and the resistance to wet rubbing fastness of tea leaves is improved; hydroxypropyl guar gum trimethyl ammonium chloride, trehalose, glucosamine sulfate, 2,4,6-trichlorine benzoic acid and 4-hydroxy phenethylamine enhance the antibacterial property of chitosan, and the dyeing firmness is facilitated; ethylene diamine tetraacetic acid, L-cysteine, 4-hydroxyphenyl boronic acid, methoxyphenylboronic acid and hexamethylol melamine enhance the fixation effect of a tea dye, and the color fastness is enhanced; the costume fabric has high resistance to washing fastness, antibacterial property and resistance to dry and wet rubbing fastness.