The invention relates to a preparation method and application of 6-methyl-3, 5-dioxocapryloyl
coenzyme A. According to the preparation method, 2-methylbutyric acid serves as a starting
raw material, the 6-methyl-3, 5-dioxocapryloyl
coenzyme A is chemically synthesized through the steps of activation, condensation,
coenzyme A coupling and the like, and the structure and purity of the 6-methyl-3, 5-dioxocapryloyl coenzyme A are identified through the technologies of LC-MS / MS, 1HNMR and the like. The nine-carbon coenzyme A is successfully prepared and identified through a
chemical synthesis method for the first time, the core problem that an intermediate is unstable and cannot be obtained
in vitro is solved, and a stable substrate is provided for in-vitro acyl transfer; the QS-21 in-vitro acyl transfer reaction is realized for the first time, an in-vitro catalytic
system of a
chemical synthesis intermediate and an
insect cell expression
enzyme is established, and a foundation is laid for development of a QS-21
total synthesis process.