11results about How to "Resolve aggregation" patented technology

The invention relates to the technical field of chemiluminescence immunoassay, in particular to a preparation method and application for solving low coating amount aggregation of carboxyl magnetic beads. Activating agents EDC and NHS are added into a first magnetic bead resuspension; cleaning the activated first magnetic bead resuspension with a coupling buffer solution to obtain a second magnetic bead resuspension with the concentration of 5-20 mg / mL; adding activators EDC and NHS and an antibody into the second magnetic bead resuspension; a first sealing agent with the pH being 6-8 and containing 50-400 mmol / L of ethanolamine is added into the second magnetic bead resuspension for primary sealing; removing the supernatant of the second magnetic bead resuspension after primary sealing, and adding a second sealing agent for secondary sealing; and washing the second magnetic bead resuspension after secondary sealing by using a magnetic bead working solution, and fixing the concentration of carboxyl magnetic beads to 0.4 mg / mL. The carboxyl magnetic bead coating process is optimized, the magnetic bead activity is improved, and the test background signal value is reduced.

The invention belongs to the technical field of immunohistochemical detection, and discloses a modified composite sealing buffer solution as well as a preparation method and detection application thereof. The modified composite blocking buffer solution comprises a basic buffer system, a composite blocking agent, a nonionic detergent, an endogenous interferent blocking agent and an antibody stabilizer, wherein the basic buffer system provides a stable pH environment and ionic strength; the composite sealing agent firstly occupies a shielding site, and the background is lowered; the nonionic detergent destroys hydrophobic interaction, effectively elute and loosen adsorbed molecules, and promote dissolution of components with relatively strong hydrophobicity; the endogenous interferent blocker can be combined with a receptor firstly, so that non-specific combination of an antibody and the receptor is blocked, and the problem of specific background is solved; the antibody stabilizer can protect the activity of the detection reagent and maintain the stability, and the obtained modified composite sealing buffer solution and the kit thereof have the advantages of multiple cooperative sealing mechanisms, prospective blocking of endogenous interference, improvement of the stability, compatibility and high efficiency of the antibody and the like.

The invention belongs to the field of biomedical materials, and provides a preparation method of an ultrasonic response nano composite piezoelectric film, which comprises the following steps: preparation of a KNN nanowire precursor, preparation of a KNN nanowire, preparation of a KNN (at) PDA nanowire, preparation of a PCL / KNN (at) PDA solution and preparation of a PCL / KNN (at) PDA film. According to the preparation method, the piezoelectric film is prepared by using the polycaprolactone and the KNN nanowire which are relatively good in biocompatibility and degradability, so that the risk of a secondary operation is avoided; through the PCL / KNN (at) PDA film, regulation and control of electric energy output by adjusting external ultrasonic pulses are realized; the PDA is modified on the surface of the KNN nanowire, so that the aggregation problem in a PCL matrix is avoided.

The invention provides a method for improving the fermentation level of avilamycin, which comprises the following steps: step 1, seed tank culture: preparing a spore solution from maturely cultured streptomyces viridochromogenes, and inoculating the spore solution into a seed tank to obtain a mature seed solution; step 2, culturing in a fermentation tank: inoculating the grown mature seed liquid into a fermentation culture medium according to an amount which is 10 percent of the volume of the inoculated mature seed liquid in the fermentation tank, and supplementing an L-valine solution, a sodium acetate solution, phytine turbid liquid and liquefied starch in the culture process, so as to obtain avilamycin fermentation liquid. According to the method, key supplements including phytin and sodium acetate are supplemented, the utilization efficiency of avilamycin A precursors and carbon sources is effectively improved in the avilamycin fermentation process through the two key supplements, the fermentation level is improved to 7329 mu g / mL from 6126 mu g / mL, the fermentation level is improved, the maximum single impurity content is effectively controlled, and finally the quality of fermentation liquor is greatly improved.

This application relates to the field of aquaculture monitoring technology, and provides a method and corresponding product for estimating fish population size based on forward-looking sonar data. The method includes: acquiring sonar monitoring data obtained from a forward-looking sonar device monitoring a target water area, and constructing an effective monitoring area in the sonar monitoring data according to the field-of-view parameters of the forward-looking sonar device; preprocessing the sonar monitoring data located within the effective monitoring area to enhance the distinguishability of fish targets; identifying fish targets located within the effective monitoring area based on the preprocessed sonar monitoring data, and generating an initial population estimate based on the number of identified fish targets; acquiring aggregation characteristics reflecting the aggregation state of fish within the effective monitoring area; and correcting the initial population estimate based on the aggregation characteristics of fish within the effective monitoring area to obtain a corrected fish population estimate.

The invention discloses a high-speed shaft-mounted cast steel brake disc material and its preparation method and application, and belongs to the field of metallurgical technology. A high-speed shaft-mounted cast steel brake disc material comprises the following components in percentage by mass: C: 0.31-0.39%; Si: 0.36-0.42%; Ti: 0.87-1.56%; Cr: 0.90-1.75%; Ni: 1.66-2.54%; Ta: 1.03-1.57%; Al: 0.006-0.009%; N: 0.009-0.016%; P: ≤0.01%; S: ≤0.01%; the balance is Fe. The high-speed shaft-mounted cast steel brake disc material obtained by the invention has the characteristics of good heat dissipation performance, excellent high-temperature mechanical properties and wear resistance.

The invention relates to a drilling pump water feeding and air exhausting device which comprises a liquid collecting tank, a fluid pipe and an air exhausting pipe, the bottom of the liquid collecting tank is connected with the fluid pipe, the air exhausting pipe is vertically arranged on the fluid pipe, the tail end of the fluid pipe is connected with a circulating fluid low-pressure side water feeding pipe, and the liquid collecting tank, the fluid pipe and the air exhausting pipe are integrally arranged in a U shape. The exhaust system does not need power, the exhaust pipe is additionally arranged at the water feeding filter screen of the drilling pump, the U-shaped pipe principle is utilized, gas gathered in a water feeding pipeline can be exhausted in time, the flow and liquidity of the low-pressure side of the pump are not affected, and therefore the problem of gas gathering in the pipeline on the low-pressure side of fluid is solved, the water feeding efficiency of the fluid on the low-pressure side is stabilized, and the service life of the drilling pump is prolonged. Design parameters of the high-pressure side are guaranteed, and drilling time efficiency is improved.

The invention relates to the technical field of humidifying fans, in particular to a control method for a humidifying fan, a computer storage medium and the humidifying fan, and aims to solve the problem that water drops gather at a mist outlet and drop after an existing humidifying fan is used. In order to achieve the purpose, according to the control method for the humidifying fan, the humidifying fan comprises a fan head, an atomization device and an atomization fan, the atomization device is provided with a mist outlet pipe fixed to the fan head, and the atomization fan and the mist outlet pipe are located in the same channel; the control method comprises the steps that after the atomization device is closed, the fan head is controlled to rotate to the preset anti-dripping position where the mist outlet of the mist outlet pipe inclines upwards; and the atomization fan is controlled to operate so as to blow-dry the mist outlet. When the atomization device is closed, the fan head automatically rotates to the preset anti-dripping position, so that water drops gathered at the mist outlet flow back, the atomization fan is started to supply air, residual water drops are helped to be removed, and the dripping phenomenon is avoided.

This invention discloses a cross-platform dynamic evaluation method and system for public opinion based on real-time semantic perception, belonging to the field of online public opinion data processing. The method includes the following steps: collecting multi-platform, multi-modal, heterogeneous data; standardizing the data; semantically aggregating the standardized cross-platform multi-modal data corresponding to each propagation event based on an event semantic center vector model; iteratively updating the event semantic center vector through online exponential sliding updates to address the dynamic evolution of propagation events, continuously supplementing the event semantic center vector with newly added standardized data related to the propagation event in real time; quantitatively evaluating the comprehensive performance of each propagation event on different platforms based on a platform performance index model, and constructing a cross-platform content ranking based on the comprehensive performance using a multi-attribute normalized decision function; and intuitively presenting the cross-platform online public opinion analysis results using a three-level responsive visualization view.

The application provides a reversible grinding automatic line suitable for H-shaped steel products, comprising a processing chamber and a conveying line passing through the processing chamber; a robot and a material turning mechanism are arranged in the processing chamber, the robot and the material turning mechanism are respectively located on two sides of the conveying line, and an end of the robot is provided with a polishing part; a first linear module is arranged at the bottom of the robot; a second linear module is arranged at the bottom of the material turning mechanism; the material turning mechanism comprises a circular seat and a power part for driving the circular seat to rotate, one side of the circular seat is provided with a side groove; a scraper is arranged in the side groove, and the scraper is connected with a driving mechanism, and the driving mechanism is used for driving the scraper to move in translation along the side groove. The scraper and the matching driving mechanism are arranged in the side groove of the material turning mechanism, after the polishing work is completed, the driving mechanism can drive the scraper to move in translation along the side groove, and the polishing debris, dust and other foreign matters accumulated in the side groove are pushed and scraped out, thereby fundamentally solving the problem of foreign matter accumulation in the groove of the existing material turning mechanism.

The invention relates to an air cooling heat dissipation structure of an elevator energy recovery device. The air cooling heat dissipation structure comprises airflow holes, a shell, deflection baffles and a cabinet door. The shell is composed of a cuboid-shaped shell body and a top cover plate, and the top cover plate is welded to the top of the cuboid-shaped shell body. Cabinet doors are hinged to two sides of the shell; airflow holes are formed in the top bottom of the shell and the cabinet doors; the deflection baffles are arranged on the inner wall of the shell and arranged on the same vertical plane in a vertically-spaced and crossed mode. The system has the beneficial effects that airflow generated when the elevator runs in the hoistway is adopted as a cooling medium of the energy storage battery, the heat exchange effect is high, temperature distribution is uniform, and cost is zero; heat generated in charging and discharging of the energy storage battery is quickly brought out of the equipment by airflow, so that the problem of heat accumulation of the equipment is effectively solved with zero cost, the energy-saving device is more energy-saving, and meanwhile, the structure is simple in process; the deflection baffles are arranged on the inner wall of the shell and are arranged on the same vertical plane in a vertically-spaced and crossed mode, the flowing time and distance of airflow in the shell are prolonged, and heat exchange is more sufficient.