The invention belongs to the technical field of immunohistochemical detection, and discloses a modified composite sealing
buffer solution as well as a preparation method and detection application thereof. The modified composite blocking
buffer solution comprises a basic buffer
system, a composite blocking agent, a nonionic detergent, an endogenous interferent blocking agent and an
antibody stabilizer, wherein the basic buffer
system provides a stable pH environment and
ionic strength; the composite sealing agent firstly occupies a shielding site, and the background is lowered; the nonionic detergent destroys hydrophobic interaction, effectively elute and loosen adsorbed molecules, and promote
dissolution of components with relatively strong hydrophobicity; the endogenous interferent blocker can be combined with a
receptor firstly, so that non-specific combination of an
antibody and the
receptor is blocked, and the problem of specific background is solved; the
antibody stabilizer can protect the activity of the detection
reagent and maintain the stability, and the obtained modified composite sealing
buffer solution and the kit thereof have the advantages of multiple cooperative sealing mechanisms, prospective blocking of endogenous interference, improvement of the stability, compatibility and high efficiency of the antibody and the like.