The invention discloses a shrinking model for developing and characterizing a
biological agent freezing and thawing process and application of the shrinking model, the volume of the shrinking model is 100-1000mL, when the shrinking model is vertically placed, the ratio of the sectional area value of the maximum section in the horizontal direction to the length value in the vertical direction is (1-10): 1, the amount of a
biological agent is 20-80% of the volume of the shrinking model, and the amount of the
biological agent is 10-20% of the volume of the shrinking model. And the ratio of the sectional area value of the maximum section to the liquid level height value is (1-15): 1. After the biological agent is loaded into the
reduced model, transferring into a refrigerator from
room temperature, after complete freezing, taking out, thawing at
room temperature, testing freeze concentration factors, and recording ice-water interface coexistence duration. According to the shrinking model, the freezing
concentration factor is compared with the freezing
concentration factor for comparison in the large-volume freezing and thawing process, the shrinking model with the freezing
concentration factor larger than or similar to the freezing concentration factor for comparison is obtained, and the ice-water interface coexistence time is not shorter than that of a large-volume container due to multiple rounds of freezing and thawing; the method is used for subsequent biological agent
freeze thawing process development and characterization. The small model is small in biological agent dosage,
temperature control equipment does not need to be used, and the research and development cost can be greatly reduced.