1results about How to "Increase particle concentration" patented technology

The present application relates to a preparation method of exosomes, comprising the following steps: 1) concentrating cell supernatant to obtain a concentrated solution by using a tangential flow filtration method; 2) removing nucleic acids from the concentrated solution by using a universal nuclease and resuspending to obtain a crude extract; 3) performing density gradient centrifugation on the crude extract, and collecting the exosome layer; 4) performing low-speed centrifugation on the exosome layer under a centrifugal force less than or equal to 50000g, and removing the precipitate to obtain a preliminary purified solution; and 5) performing ultracentrifugation on the preliminary purified solution under a centrifugal force greater than or equal to 100000g, and the obtained precipitate is the exosomes. The exosomes obtained by the method have a complete and un-deformed membrane structure, higher purity and particle concentration, and better quality compared with exosomes prepared by existing methods. Meanwhile, the method has high yield and low cost, and helps to realize the industrialized preparation of exosomes, and provides strong support for targeted drug delivery research using exosomes as carriers.