The invention relates to a micro-fluidic
chip for heterogeneous
cell electrofusion, which is characterized in that a partition wall and two micro-channels are arranged in the
chip, the two micro-channels are bilaterally symmetrical by taking the partition wall as a symmetry axis, and each micro-channel is provided with a
main channel and a bypass channel; a plurality of capturing cavities are formed in the
main channel; the bypass channel is shunted from the
main channel at the inlet of each capturing cavity, extends towards the direction far away from the main channel and then turns back in a detour manner, and the bypass channel and the main channel are converged at the outlet of the capturing cavity; a fluid resistance difference exists between the main channel and the bypass channel, so that a
cell suspension preferentially enters the capturing cavity of the main channel to capture a single
cell when entering; the capturing cavities which are symmetrical left and right are communicated through a
pairing channel, so that the two captured cells are in
close contact and are fused under the action of a pulse
signal to form a binuclear heterogeneous cell. Through special flow channel
geometric design and fluid resistance matching, efficient and accurate
pairing and electric fusion of heterogeneous cells are realized, and the method has the advantages of
high flux, good
fusion rate, less non-specific fusion and the like.