82 results about "Bioaerosol" patented technology

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.

The invention relates to the technical field of atmospheric biological aerosol sampling and detection, in particular to a particle size resolution type aerosol in-situ sampling and analysis integrated system and method. The system comprises an electrostatic field grading aerosol sampler, a micro-fluidic detection chip based on an immunochromatography principle and an image acquisition and data analysis unit, according to the electrostatic field grading aerosol sampler, biological aerosol particles are electrified through a charge tube, and grading capture according to particle sizes is realized in a stepped electrostatic field; the micro-fluidic detection chip transfers an enriched sample to a multi-layer structure micro-fluidic chip, and a specific reaction is completed based on an immunochromatography principle; and the image acquisition and data analysis unit captures a color developing image through a high-definition camera, and adopts an RGB analysis algorithm to realize quantitative detection. The method realizes full-process automation from sampling to analysis, solves the problems of low efficiency and tedious operation of a traditional method, and has the advantages of high detection speed, high sensitivity, no need of harmful reagents, environmental friendliness and the like.

The embodiment of the invention discloses a biological aerosol real-time identification method and system based on ultraviolet laser induced multi-dimensional optical fingerprints. The method comprises the following steps: irradiating biological aerosol particles with ultraviolet laser with a set wavelength, scattering the ultraviolet laser under the action of the particles, and exciting the particles to generate fluorescence; mie scattering signals are collected in the first optical channel and used for measuring physical size information of particles; a broadband fluorescence signal is collected in a second optical channel and is used for determining chemical composition information of the particles; a fluorescence signal of a selected wavelength characteristic peak value is collected in a third optical channel and is used for determining microenvironment and conformation information of particles; the horizontal component intensity and the vertical component intensity of the fluorescence signal with the set wavelength are collected in a fourth optical channel and are used for determining the structural order information of the particles; based on the obtained physical size information, chemical composition information, microenvironment and conformation information and structural orderliness information, particle classification information in the biological aerosol is analyzed and determined.

A particulate matter detection device takes holographic images of flowing particulate matter concentrated by a virtual impactor, which selectively slows down and guides larger particles to fly through an imaging window. The flowing particles are illuminated by a pulsed laser diode, casting their inline holograms on a CMOS image sensor in a lens-free mobile imaging device. The illumination contains three short pulses with a negligible shift of the flowing particle within one pulse and triplicate holograms of the same particle are recorded at a single frame revealing different perspectives of each particle. A deep neural network classifies the particles based on the acquired holographic images. The device was tested using different types of pollen and achieved a blind classification accuracy of 92.91%. This mobile and cost-effective device weighs ˜700 g and can be used for label-free sensing and quantification of various bio-aerosols over extended periods.

One variation of a method includes, during a test period: triggering release of a tracer test load into air in an environment, according to a set of release parameters, by a dispenser arranged within the environment, the first tracer test load comprising a first concentration of tracers of a first type in solution; and triggering an air sampler, located in the environment, to record a timeseries of aerosol data representing amounts of aerosol particles detected at the air sampler during the test period. The method further includes: deriving a tracer signal, representing changes in amounts of tracers in air detected at the air sampler during the test period, based on the timeseries of aerosol data and the set of release parameters; based on characteristics of the tracer signal, characterizing a set of aerosol flow metrics representing behavior of aerosols in the environment during the test period.

The application discloses a kind of distributed farm biological aerosol concentration simulation and health risk early warning method based on deep learning, comprising the following steps: constructing and deploying distributed sensor network to collect data and processing, based on deep learning method, biological aerosol concentration distributed model and health risk early warning model are constructed, the simulation and prediction result of the biological aerosol concentration distributed model is generated with the health risk grade and early warning information generated by the health risk early warning model through central processing control and 3D visualization unit 3D diagram of visual generation is generated.The application greatly reduces the risk of pathogen transmission, significantly improves the safety level of farm environment, and provides an intelligent and systematic solution for protecting the health of staff and animals.

One variation of a method includes, during a calibration period: triggering collection of an initial bioaerosol sample by an air sampler located in an environment; and triggering dispensation of a tracer test load by a dispenser located in the environment; accessing a detected barcode level of a barcode detected in the initial bioaerosol sample; accessing a true barcode level of the barcode contained in the tracer test load; and deriving a calibration factor for the environment based on a difference between the detected barcode level and the true barcode level. The method further includes, during a live period succeeding the calibration period: triggering collection of a first bioaerosol sample by the air sampler; accessing a detected pathogen level of a pathogen detected in the first bioaerosol sample; and interpreting a predicted pathogen level of the pathogen in the environment based on the detected pathogen level and the calibration factor.

This invention discloses an absolute quantification method for pathogens and drug resistance genes in atmospheric bioaerosols and its application. Addressing the bottleneck of achieving absolute quantification of low-concentration samples while maintaining cell integrity and high-concentration nucleic acid extraction, this invention constructs a collection carrier by immobilizing a hydrophilic mixed cellulose ester microporous membrane on a technical agar plate. This is combined with a flow rate ≤30 L / min impactor sampler, ensuring structural integrity while capturing microorganisms, laying the foundation for flow cytometry quantification. This front-end collection system is deeply coupled with a dedicated cascade lysis and concentration process, enabling the acquisition of high-quality DNA suitable for metagenomic library construction and relative abundance analysis even under short-term, low-flow-rate collection. Simultaneously, the total microbial count is absolutely quantified using flow cytometry on the collected samples, and this result is fused with the relative abundance results. Through synergistic optimization of the entire chain, quantitative analysis of species-level pathogens and drug resistance genes in atmospheric bioaerosols is ultimately achieved.

The invention discloses a test device for researching dynamic migration of aerosol. The test device comprises an experiment device body, and a smoke generator, heat source equipment, a PIV test control device, a simulation adjusting mechanism and an adjusting spraying mechanism are arranged in the experiment device body; according to the test device for researching the dynamic migration of the aerosol, the smoke generator, the heat source equipment, the PIV test control device, the adjusting injection mechanism and the simulation adjusting mechanism are integrated in the test device body; the whole process of aerosol generation, temperature field simulation, flow field visualization measurement and dynamic movement and posture adjustment is integrated, the smoke generator stably outputs tracing smoke or biological aerosol, the heat source equipment accurately controls the indoor thermal stratification environment, the PIV test control device obtains a high-resolution velocity vector field and a vortex structure in real time, and the measurement precision is improved. The angle and the direction of an injection head can be flexibly adjusted by adjusting a multi-stage electric push rod system of the injection mechanism.

The invention discloses a residential building toilet fresh air exhaust control system and an intelligent control method. The method comprises the steps that data of a door state sensor, a closestool state sensor, a hydrogen sulfide sensor and a biological aerosol sensor are collected; the data is transmitted to the intelligent gateway through a communication link; performing multi-source data fusion analysis by the intelligent gateway, and identifying a toilet flushing event, a water seal damage risk and a pollutant concentration trend; according to event recognition and trend prediction results, an exhaust fan and a fresh air fan are controlled to operate in advance, and rapid discharge of pollutants and indoor positive pressure maintenance are achieved; and when the water seal damage risk is detected, positive pressure air supply, pollution gas discharge and water replenishing prompts are triggered. Prediction and intervention control can be carried out before pollutant aerosol is generated, and the ventilation efficiency and the prevention and control effect are remarkably improved.

The invention provides a method for enriching bacteria and fungi in atmospheric environment biological aerosol. Research finds that when the filter membrane is used for enriching bacteria and fungi in the atmospheric environment biological aerosol, the surface of the filter membrane is coated with a protective agent, the enrichment number of the bacteria and fungi in the biological aerosol can be increased, the activity of the bacteria and fungi in the biological aerosol after enrichment can be maintained, and the protective agent comprises glycerin and / or trehalose. According to the method, the enrichment efficiency of bacteria and fungi in the biological aerosol can be remarkably improved, and the method has a wide application prospect in the aspect of subsequent sequencing analysis of microorganisms in the biological aerosol.

A photothermal composition configured to be used in air filter for destroying bioaerosol particles by converting light energy emitted from a light source into heat energy including a photothermal agent is provided. The present invention also concerns an air filter for collecting the bioaerosol particles in an air flow and destroying said bioaerosol particles deposited thereon, including a photothermal agent which converts light energy emitted from a light source into heat energy.

The invention discloses a robotic wounded personnel system for quantitative evaluation of bioaerosol respiration exposure, and belongs to the technical field of medical simulation models, and the robotic wounded personnel system comprises a human body shape subsystem; the bionic breathing subsystem is arranged in the simulated human body model; the simulated lung subsystem is arranged in the simulated human body model; the rear end of the air pipe simulation structure communicates with the cylinder through a first one-way valve; an aerosol analysis unit; one end of the exhaust pipeline is communicated with the cylinder through a second one-way valve, and the other end is communicated with the air pipe simulation structure through a filter; the bionic respiration subsystem is provided with detection points, and the aerosol analysis units are connected to the detection points respectively for sampling and analyzing particle size distribution and concentration of aerosol of each detection point. The system simulates a human body breathing process in a biological aerosol environment through the machine wounded simulation model, and can realize accurate quantitative evaluation of aerosol breathing exposure in combination with the aerosol analysis unit.

This disclosure discloses an isolation device for insolating aerosol exposure, which may be used as an isolation device for a test with a biological aerosol exposure infection risk. The isolation device for insolating aerosol exposure of the present disclosure includes a cabin body; protective half suit arranged in the cabin body; a working table arranged at an accessible horizontal position of the protective half suit in the cabin body; and an air inlet device and an air exhaust device arranged on a side wall of the cabin body. By using the support structure of the cabin body and the rigid frame, flexible assembling and disassembling of the isolation device and easiness in transportation are realized; and based on design mechanisms of negative pressure control, airtight isolation and high-level personnel protection, physical isolation of high-risk operations is realized, operation personnel is effectively protected, and environmental pollution is prevented.

The invention provides a platform for testing the temperature response characteristic of a biological aerosol sensor, and relates to the technical field of environment monitoring equipment and sensor testing, and the platform comprises an environment control cabin which is used for simulating and maintaining a wide temperature and humidity condition; the to-be-tested biological aerosol sensor interface is arranged in the environment control cabin and is used for accommodating a to-be-tested biological aerosol sensor and carrying out physical and data interface with the to-be-tested biological aerosol sensor; the aerosol generation and transmission system is used for generating and stably conveying standard particles and / or biological aerosol samples with known particle sizes; and the data acquisition and control module is used for synchronously acquiring data of the environment sensor and original data such as particle size, scattered light and multispectral fluorescence output by the bioaerosol sensor to be tested, and realizing automatic control and data storage and analysis in the testing process. According to the method, a reliable data basis can be provided for performance evaluation, temperature response mechanism analysis and adaptive compensation model development of the biological aerosol sensor under different temperature and humidity conditions.

This invention discloses an automated monitoring and detection cascade control system for bioaerosols, comprising a cascaded monitoring and detection unit and an automatic control unit. The cascaded monitoring and detection unit includes: a first-level monitoring module, a bioaerosol monitor based on laser-induced fluorescence technology (aerosol fluorescent particle monitor); a second-level monitoring module, a bioaerosol live-cell monitor based on ATP fluorescence detection technology (aerosol live-cell ATP monitor); and a third-level detection module, a bioaerosol nucleic acid detector based on microfluidic PCR technology (an integrated bioaerosol detection and nucleic acid detection system). The automatic control unit uses high and low level switching signals to achieve sequential instantaneous triggering of the three levels of equipment, completing automated hierarchical monitoring and early warning of "broad-spectrum monitoring → biological confirmation → specific detection". This invention solves the problem in existing bioaerosol monitoring technologies that cannot simultaneously achieve broad-spectrum, real-time, accurate, and automated monitoring.

The invention discloses a nuclear biochemical monitoring system and method, the system comprises a nuclear radiation detection module, a chemical toxic agent detection module, a biological factor detection module, an intelligent central control unit, a monitoring terminal and a remote command platform, the nuclear radiation detection module adopts a Geiger tube sensor to detect alpha, beta, neutron and gamma rays; the chemical toxic agent detection module is used for detecting various chemical toxic agents based on an ion mobility spectrometry technology; the biological factor detection module adopts an investigation, acquisition and detection integrated system to monitor and preliminarily screen biological aerosol, the intelligent central control unit processes, studies and judges multi-source data through 5G / Beidou communication by using a data fusion algorithm, and the monitoring terminal receives and prompts a monitoring report and a disposal scheme. The remote command platform realizes data visualization, long-term storage, multi-path alarm and emergency disposal scheme generation and issuing; according to the invention, real-time, rapid and cooperative monitoring and intelligent grading response of nuclear, biological and chemical threat factors are realized.

The utility model discloses a remote control bioaerosol generating and collecting device, which relates to the technical field of microenvironment laboratories and comprises a remote control trolley, a lifting rod, a generator and a sampler, the lifting rod is arranged on the remote control trolley, the generator and the sampler are arranged on the top of the lifting rod, and the sampler is arranged on the top of the lifting rod. The remote control trolley is controlled by a remote controller to move, and the lifting rod is controlled by the remote controller to ascend and descend. According to the remote control biological aerosol generating and collecting device disclosed by the utility model, microbial samples can be generated and collected at multiple positions, personnel do not need to enter an experimental space, and the working efficiency is improved.

The invention relates to the technical field of mass spectrum detection, in particular to a biological aerosol detecting, sampling and detecting integrated device and a control system, comprising a reconnaissance module for sequentially performing fluorescence characteristic detection and mass spectrum component analysis on single-particle aerosol and generating a judgment signal, and a sampling module which is in signal connection with the reconnaissance module and is used for sampling the single-particle aerosol. The sampling module is used for collecting an aerosol sample in an environment based on the judgment signal, the immunodetection module is in fluid connection with the sampling module and is used for carrying out immunoassay on the sample collected by the sampling module, and the reconnaissance module, the sampling module and the immunodetection module are sequentially connected. And the sampling module controls start and stop based on the judgment signal output by the reconnaissance module. Misjudgment possibly caused by a single detection method is effectively avoided, and more accurate decision support is provided for biological safety guarantee.

This invention relates to a high-efficiency aerosol sampling device and method, belonging to the fields of environmental monitoring and biosafety technology. It includes a U-shaped ventilation tube with a flat bottom, an air inlet at the top of the left longitudinal tube, and an air outlet at the top of the right longitudinal tube. A fan is installed at the air inlet to achieve high-flow-rate air introduction. The inner side of the flat section of the U-shaped ventilation tube is filled with sampling liquid, and a temperature control component is installed on the outer side of the flat section. This invention provides a device and method that can significantly increase the contact area through an evaporation-condensation cycle without reducing airflow, achieving high-throughput and high-efficiency microbial aerosol sampling. It solves the problems of low airflow, limited contact area between aerosol and sampling liquid, and insufficient sampling efficiency in existing aerosol sampling technologies.

The invention provides a bilayer active biological aerosol sampling membrane of a bionic peritoneum and a preparation method of the bilayer active biological aerosol sampling membrane of the bionic peritoneum, the sampling membrane is formed by compounding a functional capture layer and an activity maintaining base layer through interface crosslinking, and the functional capture layer is composed of a hydrophilic modified polypropylene porous film. The activity maintaining base layer is composed of porous hydrogel loaded with nutrient substances. The preparation method comprises the following steps: preparing the functional capture layer; preparing an activity maintaining base layer; and compounding. Through the bionic peritoneum structural design, the bioaerosol can be efficiently intercepted, and meanwhile, the activity of microorganisms in the bioaerosol can be maintained for a long time.

The invention discloses a construction method of a model for respiratory tract infection caused by inhalation of pathogenic microorganism aerosol, which comprises the following steps: inoculating respiratory tract cells to an upper chamber of a Transwell cell, culturing until a complete cell barrier is formed, and transferring the cell-loaded cell to an exposure cavity; atomizing the pathogenic microorganism suspension into aerosol, and regulating and controlling environmental parameters of the aerosol in real time; introducing the aerosol into an exposure cavity, and enabling the aerosol to be in direct contact with cells in a manner of simulating the inhalation process of the respiratory tract of a human body; and after the exposure is finished, transferring to a porous plate for continuous culture. The invention further discloses application of the model for respiratory tract infection caused by inhalation of the pathogenic microorganism aerosol. The method has the beneficial effects that the model construction process is simple and convenient, the natural infection process can be highly simulated, the method can be used for simulating a bioaerosol exposure scene after specific treatment, and the method is suitable for pathogen invasion mechanism analysis, host immune response research and efficient screening of anti-infection drugs.

The invention relates to a biological aerosol enrichment device and detection device combined system, and relates to the technical field of microbial aerosol experimental instruments, and the biological aerosol enrichment device and detection device combined system comprises a biological aerosol enrichment device and a biological aerosol detection device; the biological aerosol enrichment device comprises an enrichment fan and a virtual impactor communicated with the enrichment fan; the enrichment fan is used for sucking the biological aerosol into the virtual impactor for enrichment operation; the biological aerosol detection device comprises an early warning module for monitoring the concentration of biological aerosol in real time, a sampling module for sampling the virtual impactor, and a storage module for realizing storage of sampling liquid or sample liquid, the pump valve bottle assembly is used for directional transportation and on-off regulation and control among the modules; and the detection module is used for detecting the biological aerosol particles enriched in the sample liquid and determining the specific types of the biological aerosol particles. The invention provides an integrated system in which the enrichment device and the detection device are efficiently combined, and the full-chain detection requirement of the biological aerosol is met.

This invention discloses a bioaerosol enrichment and exposure device, comprising a main body, an enrichment chamber, an auxiliary chamber, and an exposure device. The enrichment chamber is located to the left of the auxiliary chamber. Inside the enrichment chamber are installed a water tank, condenser tubes, a virtual cutter, a rotary flow sampler, sampling bottles, and an inner frame. One set of water tanks is located below the inner frame. The rotary flow sampler is installed inside the inner frame. One set of condenser tubes is installed on top of one set of water tanks. One set of virtual cutters is installed on top of one set of condenser tubes, and all virtual cutters are located on top of the inner frame. A main airflow outlet is located on the side of each virtual cutter. This device enables rapid assessment of bioaerosol hazards while improving equipment mobility and safety, providing end-to-end technical support for accurate monitoring and risk control of low-concentration bioaerosols.

The application discloses a biological aerosol protection filtering device and relates to the biological aerosol protection field.The biological aerosol protection filtering device comprises a frame cabinet, an air inlet hole is arranged on the top plate of the frame cabinet, an air outlet hole is arranged on the bottom plate of the frame cabinet, and a sealed back plate is arranged in the frame cabinet; a filtering assembly is horizontally arranged in the frame cabinet; the filtering assembly vertically divides the inner cavity of the frame cabinet into four parts from high to low, and the four parts are a concentration detection cabin, a primary sterilization cabin, a deep sterilization cabin and an exhaust cabin in sequence; the biological aerosol concentration is monitored in real time by means of a gas sensor, the environmental risk grade is judged, and the working mode is adjusted according to the risk grade.

The wet-wall cyclone cup biological particle collection efficiency evaluation method disclosed by the embodiment of the invention comprises the following steps: S1, arranging a biological aerosol closed experiment module, and arranging a wet-wall cyclone cup sampler and a six-stage Anderson sampler in the experiment module; s2, generating biological aerosol with set concentration in the experiment module; s3, simultaneously starting the wet wall cyclone cup sampler and the six-stage Anderson sampler for sampling; and S4, determining sampling results of the wet-wall cyclone cup sampler and the six-stage Anderson sampler, and determining the biological particle collection efficiency of the wet-wall cyclone cup according to the sampling results. Biological particles are directly adopted for testing, so that the collection efficiency of the wet-wall cyclone cup on the biological aerosol can be truly reflected, and evaluation deviation caused by characteristic difference between physical particles and actual biological particles is avoided; the method is simple, easy to repeat, simple in equipment, high in practicability and good in universality.

This application discloses an intelligent detection system for aerosol pathogens based on real-time air quality monitoring, relating to the fields of bioaerosols and air quality monitoring. The system includes: a mobile unit that patrols along a preset route; an air quality monitoring unit that collects air quality data from each sampling point; an aerosol collection unit that collects aerosol samples when air quality data exceeds a threshold; a nucleic acid detection unit that uses a detection kit to detect the aerosol samples; and an intelligent control unit that, when air quality data exceeds the corresponding threshold, activates the aerosol collection unit to continue patrolling along the preset route; activates the nucleic acid detection unit to perform pooled testing of samples; if the pooled test result exceeds the pathogen threshold, the system revisits each contaminated area to collect aerosol samples and activates the nucleic acid detection unit to perform individual sample testing; if the individual test result exceeds the pathogen threshold, the system uploads the data to the terminal for early warning. This application enhances the accuracy and timeliness of target pathogen detection.

The application relates to the technical field of vehicles and discloses a variable-air-volume in-vehicle pressurization nuclear and biological and chemical protection system and a control method and device thereof. The system comprises a nuclear and biological protection system controller, a filter-removing ventilation unit, an overpressure control unit, a nuclear and biological monitoring unit, an oxygen concentration sensor and other vehicle-mounted system parts; the filter-removing ventilation unit is composed of at least a pressurization fan and a filter absorber; the overpressure control unit is composed of at least a differential pressure monitoring component and a pressure relief valve; the nuclear and biological monitoring unit is composed of at least a nuclear radiation monitoring subunit, a chemical toxicant monitoring subunit and a biological aerosol monitoring subunit; and the other vehicle-mounted system parts at least include a VCU, a seat pressure sensor, an air conditioner controller, an internal-external circulation switching actuator and an oxygen generator. The application can at least alleviate the problem that the existing nuclear and biological protection system lacks monitoring of the oxygen concentration in the vehicle and cannot accurately control the air volume of the pressurization fan according to the oxygen concentration in the vehicle, thereby being beneficial to guaranteeing the personal safety of combat personnel.

This invention proposes a superhydrophilic coating for wet-wall cyclone sampling of bioaerosols and its preparation method. By superhydrophilically modifying the inner wall of the sampling cup made of glass or plastic, the cyclone layer is less prone to breakage during sampling. As the liquid level decreases during sampling, the previously exposed portion above the cyclone layer is covered by a thin liquid film due to the superhydrophilic properties. This allows aerosol particles to be effectively captured upon impact with the liquid film, preventing them from bouncing off the exposed glass or plastic surface and escaping with the airflow. This method effectively solves the wall loss problem in low-flow-rate sampling and long-term sampling, thereby achieving efficient aerosol-water-solvent transfer of bioaerosol particles. It can significantly improve the sampling efficiency of wet-wall cyclone bioaerosol samplers and has broad application prospects.

The invention discloses an operating room intelligent purification control method and system, and relates to the technical field of operating room purification control, and the method comprises the steps: employing a multi-modal information fusion method, carrying out the synchronous collection and time-space alignment of an intraoperative video stream, a medical instrument starting and stopping signal, and anesthesia monitoring data, analyzing the operation behavior through a pre-trained deep neural network, and outputting an operation stage label; on the basis of the operation stage label, in combination with a three-dimensional concentration field monitored by a distributed particle sensor and a personnel movement track obtained by infrared / UWB, the space-time coincidence degree of particle concentration gradient change and personnel movement is calculated, the pollution occurrence probability is determined, and a pollution hot spot dynamic distribution diagram is generated; according to the pollution hot spot dynamic distribution map, air sampling is conducted above the air return area and the operation area, a biological aerosol fluorescence detection technology is adopted for recognizing microorganism endogenous fluorescence signals, the active microorganism equivalent concentration is obtained, and a biological risk weight coefficient is output.