Algistat

A technology of anti-algae agent and scallion, which is applied in biocides, disinfectants, animal repellents, etc.

Active Publication Date: 2013-07-03
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, aquatic plants are limited by seasons and climates, so there are certain limitations in the practical application of aquatic plants to inhibit algae.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take the fresh scallion scallion, peel it, grind it, and use the ratio of scallion scallion and organic solvent (anhydrous methanol, anhydrous acetone, anhydrous petroleum ether, 75% ethanol) to be 100g: 400mL. After leaching for two days, filter, collect the filtrate, concentrate under reduced pressure and evaporate the organic solvent, put the crude extract in a beaker with constant weight, evaporate to constant weight, and obtain the crude extract of light white anhydrous methanol, light white anhydrous acetone Crude extract, anhydrous petroleum ether crude extract of scallion white, ethanol crude extract of scallion white.

Embodiment 2

[0038] Dilute the anhydrous acetone crude extract of scallion white to 10% with anhydrous acetone, put it into the dropping funnel, and drip it to the chromatography column (macroporous resin XAD1180) from the dropping funnel. Wash the column until the distilled water flowing out of the column is mixed with 95% ethanol (ml / ml) without turbidity, and then use 2BV of acetone for elution, and the elution rate of the eluent in the column is constant at 0.5BV / h, and then concentrated under reduced pressure and evaporated to dryness of anhydrous acetone to obtain light white anhydrous acetone pure extract.

Embodiment 3

[0040] Four kinds of scallion crude extracts made in Example 1 (scallion anhydrous methanol crude extract, scallion anhydrous acetone crude extract, scallion anhydrous petroleum ether crude extract, scallion crude ethanol extract) were respectively Dissolve in dimethyl sulfoxide (DMSO), control DMSO final concentration <1% (the inhibition rate of solvent DMSO aqueous solution under test conditions is 1.06%, this value is included in the error when inhibition rate is calculated) add BG- 11 The concentration of medium is 0.1g / L. Filter through a 0.22 μm microporous membrane under sterile conditions to eliminate microorganisms to obtain four sample solutions.

[0041] Take Microcystis aeruginosa in the logarithmic growth phase and put them into sterilized 100mL Erlenmeyer flasks, the final concentration of algae cells is 5.5×10 5 cells / mL, add four kinds of sample solutions respectively, so that the final volume is 50 mL, and the final concentration is 0.1 g / L. At the same tim...

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Abstract

The invention discloses an algistat, which is an absolute methanol crude extract of onion stalks, an absolute acetone crude extract of the onion stalks, an absolute petroleum ether crude extract of the onion stalks or an ethanol crude extract of the onion stalks. Compared with the prior art, the crude extracts from the onion stalks by the organic solvents perform an inhibitory effect to microcystis aeruginosa from high to low as follows: the inhibitory effect of absolute acetone is larger than that of 75% ethanol, the inhibitory effect of the 75% ethanol is larger than that of absolute petroleum ether, and the inhibitory effect of the absolute petroleum ether is larger than that of absolute methanol. The inhibition ratio of the absolute acetone pure extract of the onion stalks is enhanced along with the time, and a concentration effect is shown. The inhibition ratio of a high dose group on the fourth day can be up to 97.7%. EC50 (50% effective concentration) computed on the fifth day is 0.03g/L, which proves that the algal inhibition effect of the purified absolute acetone extract is more obvious than that before purification.

Description

technical field [0001] The invention belongs to the technical field of algicides. Background technique [0002] In recent years, with the increase of human activities, the eutrophication of lakes and reservoirs has become increasingly serious, resulting in frequent outbreaks of algae blooms and reduced utilization efficiency of water resources. As a result, the aquaculture economy has suffered huge losses, and water environment security is also facing serious challenges. threat. Therefore, effectively inhibiting the excessive growth of phytoplankton in eutrophic water bodies and preventing the occurrence of algae blooms has become a research hotspot and frontier in the field of environment. Among the many methods to control the growth of algae, the traditional physical and chemical methods of inhibiting the growth of algae will inevitably destroy the ecological balance and cause environmental pollution. The biological method, especially the use of plant allelopathy to inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/42A01N31/02A01N31/06A01P1/00
Inventor 张庭廷周杨李源刘璐李诚
Owner ANHUI NORMAL UNIV
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