Photobacterium phosphoreum and application thereof

A bright luminous bacterium, a technology for use, applied in the direction of bacteria, lyase, microorganisms, etc., can solve the problems of low production of alginate lyase, limited application, poor storage stability, etc., easy to achieve industrial scale-up production, simple ingredients, and preparation simple effect

Inactive Publication Date: 2014-05-21
ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the production of alginate lyase is low and the storage stability is poor, which greatly limits the application in industrial production.

Method used

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  • Photobacterium phosphoreum and application thereof
  • Photobacterium phosphoreum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for producing alginate lyase, the specific method is as follows:

[0037] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 27.8°C and a shaker speed of 180rpm for 14-16 h to the logarithmic growth phase to obtain a seed solution. The formula of the medium is as follows: every 100 mL of seed medium contains 1.80 g of tryptone, 0.48 g of yeast extract, 2.80 g of sodium chloride, and 0.80 g of glycerin, with a natural pH;

[0038] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, and transfer the seed liquid obtained in step (1) to the fermentation medium with an inoculum of 2.0%. 6.6. Cultivate for 22-26 hours at a shaker speed of 180 rpm to obtain a fermentation broth. The formula of the fermentation medium is as follows: Each 100mL fermentation medium contains 0.50g of glucose, 0.18g of peptone, 0.48g of sodium chloride, dihydrogen...

Embodiment 2

[0042] A method for producing alginate lyase, the specific method is as follows:

[0043] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 28.5°C and a shaker speed of 220rpm for 14-16 h to the logarithmic growth phase to obtain a seed liquid. The formula of the medium is as follows: every 100 mL of seed medium contains 2.20 g of tryptone, 0.52 g of yeast extract, 3.20 g of sodium chloride, and 1.20 g of glycerin, with a natural pH;

[0044] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, and transfer the seed solution obtained in step (1) to the fermentation medium with an inoculum of 2.0%. 7.6. Cultivate for 22~26 hours under the condition of shaker speed 220 rpm to obtain fermentation broth. The formula of the fermentation medium is as follows: every 100mL fermentation medium contains 0.70g of glucose, 0.25g of beef extract, 0.52g of sodium c...

Embodiment 3

[0048] A method for producing alginate lyase, the specific method is as follows:

[0049] (1) Inoculate the slant culture of Photobacterium lumina SRU-2 into the seed medium, and cultivate it at a temperature of 28.0°C and a shaker speed of 200rpm for 14-16 h to the logarithmic growth phase to obtain a seed liquid. The medium formula is as follows: Each 100mL seed medium contains tryptone 2.00g, yeast extract 0.50g, sodium chloride 3.00g, glycerin 1.00g, and the pH value is natural;

[0050] (2) Prepare the fermentation medium according to the ratio of 30mL fermentation medium to 500mL shake flask, transfer the seed solution obtained in step (1) to the fermentation medium with an inoculum of 2.0%, and keep the temperature at 28.0°C and pH value 8.6. Cultivate for 22-26 hours under the condition of shaking table rotation speed 200 rpm to obtain fermentation broth. The formula of the fermentation medium is as follows: each 100mL fermentation medium contains 0.85g of glucose, 0.2...

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Abstract

The invention relates to a microorganism bacterial strain and application thereof, and particularly relates to a photobacterium phosphoreum and application thereof, overcoming the defects that no report about photobacterium phosphoreum which is used in production of alginate lyase in the prior art is available, the alginate lyase produced by other bacterial strains has low output and is poor in storage stability, which greatly limits the use in industrial production. The photobacterium phosphoreum SRU-2 is preserved in China Center For Type Culture Collection on December 29, 2013 with a preservation number of CCTCC M2013723. The bacterial strain can be used for producing alginate lyase. The alginate lyase produced by the photobacterium phosphoreum has high output, and the alginate lyase liquor has good storage stability, so that the application range of the alginate lyase in industrial production is greatly enlarged.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a bright photobacterium and its application. Background technique [0002] Alginate lyase (alginate lyase) specifically acts on 1 → 4 glycosidic bonds, and catalyzes the degradation of alginate through the β-elimination mechanism to produce uronic acid oligosaccharides containing unsaturated bonds and uronic acid monomers with unsaturated bonds. Since no hydrolase that can act on alginate has been found, alginate lyase is currently the only effective alginate-degrading enzyme. According to its substrate specificity, alginate lyase is divided into three categories. The first category can degrade Mannocuronic acid (PM), known as PM enzyme (EC 4.2.2.3); the second type can degrade guluronic acid (PG), known as PG enzyme (EC 4.2.2.11); the third type is bifunctional Enzymes can degrade both PM and PG. [0003] Alginate lyase is not only used as a tool enzyme for the prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/88C12R1/01
Inventor 刘彩琴陈蔚青蔡成岗王楠陈虹金建昌
Owner ZHEJIANG SHUREN UNIV
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