Streptomyces microflavus solid fermentation medium and preparation method thereof, streptomyces microflavus fermentation method and fermentation product, bio-control products of fermentation product as well as application of fermentation product and bio-control products
A technology of Streptomyces flavus and solid fermentation, which is applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc. The number of viable bacteria and the amount of spores produced are small, so as to achieve the effects of good bacterial activity and quality stability, simple recovery and purification process and waste treatment, and short fermentation cycle.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0073] According to a second aspect of the present invention, the preparation method of the above-mentioned Streptomyces flavinus solid fermentation medium is provided, comprising the following steps:
[0074] The bran, rice husk, rice bran, calcium carbonate and water are uniformly mixed in a formula amount, and sterilized to obtain a solid fermentation medium of Streptomyces luteus.
[0075] The medium preparation method is simple and suitable for large-scale production.
[0076] Preferably, the sterilization temperature is 110-130°C, and / or the sterilization time is 30-60min, preferably 40min at 121°C.
[0077] Typical but non-limiting examples of sterilization temperatures are 110°C, 115°C, 120°C, 125°C or 130°C.
[0078] Typical but non-limiting sterilization times are, for example, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min.
[0079] After being sterilized at a certain temperature, a sterile Streptomyces flavinus solid fermentation medium is obtained.
...
Embodiment 1
[0147] A fermentation method of Streptomyces flavinus, comprising the following steps:
[0148] (a) slant seed activation: Streptomyces flavinus ACCC 40027 was inoculated under aseptic conditions in the soluble starch of 8g / L, the glucose of 12g / L, the yeast powder of 2g / L, the dipotassium hydrogen phosphate of 1g / L , 0.5g / L of magnesium sulfate and 25g / L of agar, pH 7.0, sterilized at 121°C for 25 minutes on a prepared slant medium, cultivated at 28°C, and cultivated for 120h to obtain slant seeds;
[0149] (b) Liquid seed culture: elute the slant seeds obtained in step (a) with sterile water, and use a sterile pipette to inoculate into 8g / L peptone, 12g / L yeast extract powder, 15g / L Glucose and 10g / L calcium carbonate, pH 7.0, sterilized at 121°C for 25min in the liquid medium prepared, the liquid medium is a 1L Erlenmeyer flask with a sample volume of 200mL, inoculum volume of 15mL / bottle, culture temperature at 28°C, shaker at 180r / min, culture time 48h, obtain liquid se...
Embodiment 2
[0153] A fermentation method of Streptomyces flavinus, comprising the following steps:
[0154] (a) slant seed activation: Streptomyces luteus ACCC 40027 was inoculated under aseptic conditions in 12g / L soluble starch, 8g / L glucose, 5g / L yeast powder, 0.5g / L dihydrogen phosphate Potassium, 1g / L of magnesium sulfate and 15g / L of agar, pH 7.5, sterilized at 121°C for 25 minutes on a prepared slant medium, cultivated at 30°C, and cultivated for 96h, to obtain slant seeds;
[0155] (b) Liquid seed culture: the slanted seeds obtained in step (a) were eluted with sterile water, and inoculated with 12g / L peptone, 8g / L yeast extract powder, 25g / L Glucose and 5g / L calcium carbonate, pH 7.5, sterilized at 121°C for 25 minutes in the liquid medium prepared, the liquid medium is a 1L Erlenmeyer flask with a sample volume of 200mL, an inoculum volume of 10mL / bottle, a culture temperature of 30°C, and a shaking table of 180r / min, culture time 30h, obtain liquid seed;
[0156] (c) Solid f...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com