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167 results about "Streptomyces microflavus" patented technology

Streptomyces microflavus is a bacterium species from the genus of Streptomyces which has been isolated from soil. Streptomyces microflavus produces nemadectin, fattiviracin A1, milbemycin and deoxyuridines.

Compound probiotics preparation for biological organic fertilizers and preparation method thereof

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.
Owner:周剑平 +1

Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof

The invention relates to a microbiological composite bacterial inoculum and a preparation method thereof, in particular to the microbiological composite bacterial inoculum used for preparing a biological bacterial fertilizer. The microbiological composite bacterial inoculum is prepared by culturing Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Bacillus subtilis and Streptomyces microflavus var. lactosus which are disclosed in a culture catalogue of the China Center for General Microbiology Culture Collection (CGMCC), wherein the serial number of the Rhodop seudanonas palustris is 1.2352; the serial number of the Candida utilis is 2.1180; the serial number of the Lactobacillus acidophilus is 1.1854; the serial number of the Bacillus subtilis is 1.1630; the serial number of the Streptomyces microflavus var. lactosus is 4.0891; and the weight ratio of the Rhodop seudanonas palustris to the Candida utilis to the Lactobacillus acidophilus to the Bacillus subtilis is 1:0.5:0.5:0.1. When used for preparing the biological bacterial fertilizer, the composite bacterial inoculum can effectively decompose and release crude fibers and other nutrient components in straws and livestock manure; and the prepared biological organic fertilizer can condition the soil, increase the activity rate of microorganisms in the soil, avoid soil hardening, improve the permeability of the soil, play a role in production increasing, and effectively solve the problem of the area source pollution of agricultural wastes in rural areas.
Owner:于和军

Liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and preparation method thereof

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.
Owner:GUANGZHOU JUCHAN MODERN AGRI RES INST CO LTD

Preparation method of probiotic inoculum for preventing replant disease of greenhouse cucumbers

The invention belongs to microbial fermentation, and particularly relates to a preparation method of a probiotic inoculum for preventing replant disease of greenhouse cucumbers. The preparation method comprises a fermentation step: inoculating a probiotic inoculum producing strain into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water; carrying out aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.2-7.6 in the fermentation process, wherein the producing strain is a mixture of Bacillus subtilis, Bacillus licheniformis, Trichosporon behrendii, Paecilomyces lilacinus, Streptomyces microflavus, Bacillus polymyxa and Metarhizium anisopliae of the same mass; and adsorbing the final fermentation product, and immobilizing on grass carbon to obtain the probiotic inoculum. The preparation method solves the defects of replant disease, root rot, blight, nematode disease and the like in the existing greenhouse vegetable-cucumber planting technology, has the advantages of low production cost, short fermentation period and high flora density, and can effectively prevent seedling death, seedling yellowing, blight, mosaic disease, root rot and other ecological symptoms of soil. Meanwhile, the preparation method can promote the growth of roots, loosen the soil, strengthen the seedlings and degrade the residual pesticides and fertilizers, and has an effect of obviously restoring the soil ecosystem. By using the preparation method, the yield of greenhouse cucumbers can be increased by 15-20% in one season.
Owner:石家庄金太阳生物有机肥有限公司

Microbial agent for promoting rootage, preventing continuous cropping and performing ecological restoration on soil for planting bananas and preparation method thereof

The invention relates to a microbial agent for promoting rootage, preventing continuous cropping and performing ecological restoration on soil for planting bananas and a preparation method thereof, and the microbial agent is prepared by inoculating a production strain into a sterile culture medium containing a carbon source, a nitrogen source, an inorganic salt and water, performing aerobic fermentation under the conditions that the temperature is 30-37 DEG C and the pH value is 7.4-7.6, performing primary amplification culture respectively, mixing primary amplification culture solutions with equal mass, further performing secondary and tertiary amplification culture, finally adsorbing fermentation end products and immobilizing on a carrier for preparing the microbial agent, wherein the production strain is selected from bacillus subtilis, bacillus licheniformis, saccharomyces bayanus, paecilomyces lilacinus, streptomyces microflavus and bacillus polymyxa. The microbial agent can be used for effectively preventing dead seedlings, yellow seedlings, wilt, leaf spots, root rot and other soil ecological diseases during the planting process of the bananas, the production cost is low, the fermentation period is short, the flora density is high, and the microbial agent can be used for further promoting the growth of roots, loosening the soil, strengthening the seedlings, removing the pollution caused by residues of pesticides and fertilizers and playing a soil ecological restoration role.
Owner:赵颖

Fermentation composite fertilizer suitable for greenhouse vegetable planting and preparation method thereof

The invention discloses a fermentation composite fertilizer suitable for greenhouse vegetable planting and a preparation method thereof, belonging to composite fertilizers, and particularly relating to an organic fertilizer with addition of bacterial cultures. The fermentation composite fertilizer is characterized by being prepared through aerobic fermentation of microbial inoculum by taking alcohol anaerobic tank mud as a main raw material; and the fermentation composite fertilizer comprises the following raw materials in weight fraction: 680-810 the alcohol anaerobic tank mud with water content of 50-55wt%, 10-15 coal ash, 60-70 coal dust, 50-60 phosphor gypsum, 3-5 potassium chloride or potassium sulfate, 9-11 urea, 45-55 ground phosphate roc, 1.5-2 streptomyces microflavus and 1.5-2 multi-element composite enzyme microbial inoculum. The invention provides the resource-saving and environment-friendliness type biological organic fertilizer which can be used for increasing vegetable quality, improving soil, supplying balanced fertilizer, controlling loss of inorganic fertilizer, protecting the environment, ensuring that vegetable products reach a green food standard and carbon-sourced substances and microbial bacteria in the organic fertilizer achieve the effects of physiological stimulation and metabolism reinforcement for crops. In addition, the organic fertilizer has complete nutrition.
Owner:山东金沂蒙生态肥业有限公司

Aerobic bacterium-anaerobic bacterium composite microbe liquid inocula and preparation method thereof

The invention discloses aerobic bacterium-anaerobic bacterium composite microbe liquid inocula and a preparation method thereof. The composite microbe liquid inocula comprise six liquid aerobic microbial agents and four liquid anaerobic microbial agents. The liquid aerobic microbial agents comprise a bacillus megaterium agent, a liquid Azotobacter chroococcum agent, a liquid bacillus mucilaginosus agent, a liquid streptomyces microflavus agent, a liquid bacillus subtilis agent and a liquid bacillus licheniformis agent. The liquid anaerobic microbial agents comprise liquid photosynthetic bacteria, a liquid lactobacillus plantarum agent, a liquid lactobacillus acidophilus agent and a liquid lactobacillus delbrueckii agent. The preparation method comprises culturing six types of aerobic bacteria to obtain six liquid aerobic microbial agents, culturing four types of anaerobic bacteria to obtain four mixed liquid anaerobic microbial agents, and injecting the six liquid aerobic microbial agents into the mixed liquid anaerobic microbial agents to obtain the composite microbe liquid inocula. The composite microbe liquid inocula have a quality guarantee period for one year or more at the normal temperature and have functions of nitrogen fixation, phosphate dissolution, potassium release, disease prevention and soil improvement.
Owner:江苏春语生物科技有限公司

Domestic garbage and sludge decomposing bacterium agent and application thereof and prepared decomposing matrix

The invention discloses a rural domestic garbage and sludge decomposing bacterium agent and a using method as well as a decomposing matrix prepared by using the bacterium agent and a specific process through fermentation. The bacterium agent is compounded by 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum, 0.5-2 parts of aspergillus nige and 0.5-2 parts of bacillus mucilaginosus in parts by weight; the effective viable count of the bacterium agent is 5*10<8>/g; the bacterium agent can be used for decomposing garbage and sludge, deodorizing, resisting fusarium wilt of cucumber and increasing the yield when being applied to crops; the fermentation period of the treated garbage and sludge is 20 days when the bacterium agent is inoculated and 30 days when the bacterium agent is not used. The decomposing matrix is prepared by adding 1 kilogram of bacterium agent into every 5 square mixture through the specific process and fermentation; the mixture is formed by mixing 40-60% of domestic garbage, 20-40% of sludge and 5-15% of corn straws in percentage by weight; when the matrix is applied in cucumber cultivation, the yield is increased by 11% per mu, the income is increased by 859 yuan, and 200mL of pesticide is saved.
Owner:天津市农业科学院

Bio organic fertilizer for aquaculture and paddy field and producing method thereof

ActiveCN101357857AQuantity ratio is stableFully exploit biological characteristicsOrganic fertilisersBiotechnologyBacillus cereus
The invention relates to a biological organic fertilizer used for aquaculture paddy fields and the production method thereof. The fertilizer is prepared by the raw materials according to the parts by weight: 0.3 to 0.5 parts of taurine, 2 to 3 parts of treptomyces hygroscopicus, 2 to 3 parts of chromagenic streptomyces aureofaciens, 2 to 4 parts of bacillus cereus, 2 to 4 parts of bacillus sphaericus, 9 to 13 parts of bacillus mucilaginosus, 2 to 3 parts of azotobacter chroococcum, 2 to 3 parts of streptomyces microflavus, 10 parts of fruit waste degradation products, 10 parts of matrix and 46.5 to 57 parts of soil. In the method, as fruit waste degradation product polysaccharide carbohydrate is mixed with nitrogen-fixing, phosphate-dissolving and potassium-dissolving active compound microorganisms and a compound actinomycete agent which has antagonistic effect on rice blast and rice sheath blight, and matched with nutritional components which stimulate the growth of fisheries in rice fields, the biological organic fertilizer can not only be used for improving soil microbial community structure, activating soil nutrients, and improving rice rhizosphere nutrition, increasing the yield and quality of rice and improving water quality, but also promote the growth of cultured fisheries in rice fields.
Owner:大智然(北京)生态农业有限公司

Coated microbial bacterial fertilizer particles and preparation method thereof

The invention belongs to the field of agricultural fertilizers and particularly relates to coated microbial bacterial fertilizer particles and a preparation method thereof.The coated microbial bacterial fertilizer particles include pill cores and coating layers, wherein the pill cores use duck manure, monosodium glutamate leftovers, furfural residues, corn cobs and plant ash as fermentation base materials, use bacillus subtilis, brevibacillus laterosporus, streptomyces microflavus, paecilomyces lilacinus, bacillus licheniformis and bacillus megatherium for aerobic fermentation and are obtained through granulation; the coating layers use agaricus bisporus residues, distillers' grains, traditional Chinese medicine residues, humic acid, guar gum and diatomite as base materials, an EM bacterial solution is added for fermentation, and then the mixture is mixed with sugar.The number of living bacteria of the coated microbial bacterial fertilizer particles is 62 millions CFU/g or above, the organic matter content is, by weight, 58.5% or above, pH is 7.1-7.2, and the content ratio of N to P to K is 2.5-3.0 to 1 to 1.8-2.1.The raw materials are easy to obtain, the preparation method of the coated microbial bacterial fertilizer particles is simple in operation, and the fermentation process is easy to control.
Owner:SHANDONG LVHENG BIOLOGICAL ENG

Method for producing microbial fertilizer by utilizing kitchen waste

The invention provides a method for producing a microbial fertilizer by using kitchen waste. The microbial fertilizer contains one selected from the group consisting of Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus megatherium, Brevibacillus laterosporus, Bacillus thuringiensis, Bacillus subtilis and Streptomyces microflavus or mixed bacteria of two or more selected from the group. The method for producing the microbial fertilizer comprises the following steps: removing foreign matters in the kitchen waste, heating the kitchen waste, taking out grease, crushing the rest kitchen waste, adding enzyme for enzymolysis, putting the kitchen waste having undergone enzymolysis into a fermentation tank, performing sterilizing, inoculating one or more culturedstrains, performing fermenting with a pure bacterium or mixed bacteria to obtain a single-bacterium liquid microbial fertilizer or a composite-bacteria liquid microbial fertilizer, and adsorbing the liquid bacterial fertilizer with a carrier to obtain the solid microbial fertilizer. The microbial fertilizer is produced by utilizing the kitchen waste, so production cost is remarkably reduced, and the pollution of the kitchen waste to the environment can be effectively reduced.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Intestine-regulating constipation-removing enzyme and preparation method thereof

The invention relates to an intestine-regulating constipation-removing enzyme and a preparation method thereof. The enzyme comprises the following raw materials in percentage by weight: 5% of figs, 10% of sophora flowers, 15% of celery, 20% of pears, 20% of apples, 2% of radix paeoniae alba, 4% of plantain, 3% of liquorice, 20% of mulberries, 2% of dandelion, 20% of flaccid knotweed herb and 80% of coarse rice. The preparation method comprises the following steps: 1. steaming the coarse rice in the raw materials, alcoholizing the steamed coarse rice and the flaccid knotweed by hansenula anomala and saccharomyces cerevisiae, and after alcoholizing, adding water, acetifying by acetobacter pasteurianus and clostridium butyricum; 2. grinding the other materials, alcoholizing by honey yeasts, hansenula anomala, rhodotorula glutinis, saccharomyces cerevisiae and streptomyces microflavus, after finishing the steps 1 and 2, mixing according to a ratio of 1 to 1, lactifying by lactobacillus lactis and plant lactobacilli, and fermenting to obtain suspension liquid as the intestine-regulating constipation-removing enzyme. The intestine-regulating constipation-removing enzyme has the effects of promoting production of body fluid, moistening the lung and facilitating intestinal tract movement, and is suitable for crowds with constipation.
Owner:SUZHOU KEDA WEILONG INFORMATION TECH

Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant

The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.
Owner:天蓝地沃(天津)农业科技有限公司

Microbial preparation for photovoltaic agriculture and production method thereof

The invention discloses a microbial preparation for photovoltaic agriculture and a production method thereof. The microbial preparation is prepared from, by mass, 10%-20% of bacillus subtilis, 10%-20% of streptomyces microflavus, 20%-30% of rhodopseudomonas palustris, 20%-30% of nanometer calcium, 5% of sulfated polysaccharides from brown and 20%-30% of microelements. The raw materials are cultivated to the logarithmic phase in a self liquid medium respectively, the fermentation product is prepared from, by weight, 10%-20% of the bacillus subtilis, 10%-20% of the streptomyces microflavus and 20%-30% of the rhodopseudomonas palustris in a mixed mode, the mixed microbial number is larger than or equal to 2*10<8>, and then the sulfated polysaccharides from brown accounting for 5% of the total mass of the fermentation product, the nanometer calcium accounting for 20% to 30% of the total mass of the fermentation product and the microelements accounting for 20% to 30% of the total mass of the fermentation product are mixed. The microbial preparation is photovoltaic-planting special microbial fertilizer, the problems caused by the novel cultivation mode are solved, and the yield and the quality of plants are improved. As various microelements are added in the microbial preparation, nutrient balance of plants can be met; Fe and Mg have the direct effects on improving the plant chlorophyll content and promoting photosynthesis.
Owner:大连市隆基微生态学应用研究院
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