14 results about "Streptomyces microflavus" patented technology

The invention discloses low-temperature salt-tolerant and alkali-tolerant streptomyces microflavus and application thereof, relates to the technical field of microorganisms, and is characterized in that the preservation number of the streptomyces microflavus is CCTCC (China Center for Type Culture Collection) M2023664. The streptomyces microflavus provided by the invention can well grow at the temperature of 10-30 DEG C. In a Congo red decoloration test, the ratio of the diameter (D) of a transparent circle to the diameter (d) of a bacterial colony is 3.6. The strain is applied to degradation of cellulose, has the advantages of degradation of cellulose at low temperature, high cellulase activity, salt resistance, alkali resistance and the like, and can be particularly used for cellulose degradation strain resources in low-temperature saline-alkali areas.

The invention discloses a wormcast organic fertilizer and a preparation method thereof, relates to the technical field of organic fertilizer preparation, and aims to solve the problems of nutrient imbalance, insufficient decomposition, poor slow release performance and the like of the existing wormcast fertilizer. The method comprises the following steps: by taking agricultural wastes such as straw powder, peanut shell powder and the like, cow dung and wormcast as raw materials, inoculating a bacillus subtilis microbial inoculum containing alginate oligosaccharide, an aspergillus sydowii microbial inoculum and a streptomyces microflavus microbial inoculum containing mannitol, performing segmented fermentation, adding a multifunctional adhesive, and granulating, thereby obtaining the feed additive. The product realizes balanced nutrients and full decomposition, has good slow release performance and high particle strength, efficiently utilizes agricultural wastes, and improves the practicability and environmental protection value of the fertilizer.

The application relates to a biological saline-alkali soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. In order to solve the problems of low activity, single function and poor aggregation promotion effect of an existing biological conditioner in a low-temperature saline-alkali environment of black soil in a cold region, the application provides a biological saline-alkali soil conditioner which comprises a multifunctional composite bacterial system Z2 composed of bacillus velezensis PZ-3, bacillus cereus K1, streptomyces microflavus CLSD-1 and pseudomonas alcaliphila WP9, and a carrier matrix auxiliary material composed of chitosan, polyacrylamide and biogas residue. The biological saline-alkali soil conditioner provided by the application has multiple functions of low-temperature saline-alkali resistance, yield increase, straw degradation and aggregation body formation, can effectively reduce the soil pH and bulk density, improve the aggregation body proportion and moisture content, significantly promote crop growth and increase yield, is suitable for the improvement of black soil and low-temperature saline-alkali soil in the northeast cold region, and is environment-friendly and free of chemical residues.

The present application relates to a kind of microbial slow-release bacterial fertilizer and its preparation method and application, belong to microbial bacterial fertilizer technical field.The microbial slow-release bacterial fertilizer described in the present application contains composite microorganism 15-18 parts, soybean root powder 8-10 parts, β-cyclodextrin 20-23 parts, grasswood ash 15-20 parts, humic acid 5-8 parts, tea saponin 5-8 parts, potassium fulvate 13-15 parts, carboxymethyl chitosan 18-20 parts by weight;Wherein composite microorganism contains round brown nitrogen-fixing bacteria, bacillus subtilis, streptomyces microflavus.The microbial composite bacterial fertilizer of the present application can effectively reduce soil acidity, improve soil structure, release nutrient components, increase soil fertility, be conducive to promoting crop growth and improving crop yield.

The invention relates to application of a streptomyces albidoflavus strain SaTC2601 to promotion of straw conversion of protaetia brevitarsis. The streptomyces albidoflavus is found to be capable of promoting the straw conversion rate of the protaetia brevitarsis larvae and promoting the increase of the protaetia brevitarsis larvae after being used for treating the straws, so that not only is the absorption of straw non-point source pollution facilitated, but also the protein yield of the protaetia brevitarsis larvae can be increased by increasing the weight of the protaetia brevitarsis larvae; therefore, a channel which is more beneficial to guaranteeing the national grain safety is provided.

The application discloses a kind of biological inoculant for preventing and treating wheat scab and a preparation method and application thereof, belong to plant disease control technical field.The microbial inoculant is prepared from the following raw materials by weight percentage: Trichoderma harzianum 5-10 parts, Streptomyces microflavus 2-5 parts, Bacillus subtilis 2-5 parts, soybean meal 75-90 parts, vitamin B1 0.1-0.5 parts, potassium fulvate 2-5 parts and ferrous phosphate 2-5 parts.The microbial inoculant of the application uses Trichoderma harzianum, Streptomyces microflavus and Bacillus subtilis as biocontrol strains, which has inhibitory effect on Fusarium asiaticum.The wheat scab control scheme of the application is environmentally friendly and sustainable.The application enhances the resistance and biodiversity of crops, reduces the dependence on chemical pesticides and promotes the balance of agricultural ecology.

The invention provides a three-river-source moderately-degraded alpine meadow microbial complex microbial inoculant synergistic reseeding rapid recovery method and application, and belongs to the technical field of soil remediation. The invention provides a microbial complex microbial inoculant and application of synergistic reseeding of the microbial complex microbial inoculant in degraded meadow repair. The microbial complex microbial inoculants comprise rhizopus intro-rhizosporium and streptomyces microflavus; the reseeded plant seeds comprise seeds of gramineous plants, leguminous plants and miscellaneous grass plants. By introducing two dominant bacteria, the utilization rate of plants to soil resources is improved, the plants have higher environmental stress coping capacity, and by means of the synergistic reseeding technology, the vegetation community composition and the species important value are improved. According to the method disclosed by the invention, the microbial complex microbial inoculants are applied to the restoration of the moderately degraded alpine meadow in the Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-

The invention discloses low-temperature glucose-tolerant beta-glucosidase and application thereof, and relates to the field of enzyme engineering. The beta-glucosidase is separated from Streptomyces microflavus CLSD-1 and belongs to a GH1 family, and the amino acid sequence of the beta-glucosidase is shown as SEQ ID No: 1 or SEQ ID No: 2. The application of the beta-glucosidase is that the beta-glucosidase is applied to simultaneous saccharification and fermentation production of ethanol from cellulose. The beta-glucosidase (Bgl2350 and Bgl5676) provided by the invention has the characteristics of low temperature and high activity, the optimal reaction temperatures are respectively 40 DEG C and 30 DEG C, and both the beta-glucosidase and the Bgl5676 have excellent glucose and xylose tolerance and can tolerate 600mM glucose and 2M xylose environments. Wherein the enzyme activity of the Bgl2350 is relatively high under a low-temperature condition; the Bgl5676 shows the stability and the salt tolerance which are obviously superior to those of the traditional GH1 family beta-glucosidase. Compared with the commercially available Novozymes Cellic CTec3 commercial enzyme, the Bgl2350 and the Bgl5676 show more prominent application potential in the field of low-temperature cellulose saccharification, and can partially replace the beta-glucosidase component in the commercial enzyme in practical application.

The application discloses a micro-white Streptomyces avermitilis strain SaTC2601 and application thereof, and belongs to the field of biological control. The micro-white Streptomyces avermitilis strain SaTC2601 is isolated from the excrement of white star flower chafer (WSFC) larvae, and has a preservation number of CGMCC No.33741. The strain has a good prevention and treatment effect on peanut white thread blight caused by Sclerotium rolfsii on peanut roots, and also has a prevention and treatment effect on soil-borne pathogenic bacteria such as Fusarium graminearum, Fusarium solani, Fusarium moniliforme, Fusarium oxysporum, Rhizoctonia solani and Streptomyces scabiei. Further, the strain SaTC2601 also has a good growth promotion effect. The strain becomes a valuable resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in soil disease prevention and control.

The invention discloses a compound bacterial fertilizer for improving drought resistance of rape and application of the compound bacterial fertilizer, and belongs to the technical field of agricultural planting. The compound bacterial fertilizer comprises 40 wt%-45 wt% of functional components and 55 wt%-60 wt% of compound fertilizer, the functional component is prepared from the following raw materials in parts by weight: 0.5 to 2 parts of paenibacillus polymyxa, 0.5 to 2 parts of streptomyces microflavus, 2 to 5 parts of trichoderma harzianum, 0.05 to 0.2 part of brassinosterol, 0.3 to 0.5 part of alginic acid and 4 to 6 parts of humic acid. The compound bacterial fertilizer provided by the invention has a remarkable effect on improving the drought resistance of the rape, and can relieve rape cell damage caused by drought, maintain a photosynthetic function, promote dry matter accumulation and improve the drought resistance and yield of the rape. The method has important practical significance for solving the problem of rape drought stress and promoting green and sustainable development of the rape industry.

The invention discloses a bio-organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizers. The preparation method comprises the following steps: respectively pretreating apple pomace, straw, cow dung and sheep manure, and mixing to obtain a mixture; bacillus subtilis, Streptomyces microflavus, Streptomyces griseus and Trichoderma harzianum are respectively cultured and then are mixed to obtain a mixed bacterial liquid. The preparation method comprises the following steps: adding a mixed bacterial solution into a mixture for high-temperature aerobic fermentation, then adding chitin for medium-temperature facultative fermentation, and finally adding selenized potassium humate and ground phosphate rock for anaerobic fermentation, thereby finally obtaining the biological organic fertilizer. The biological organic fertilizer with the characteristics of high organic matter content, high antibacterial activity and slow release of nutrients is produced within 30 days by virtue of pretreatment optimization of the raw materials, time sequence regulation and control of the complex microbial inoculants and accurate addition of synergistic substances, and the targets of waste recycling and ecological prevention and control of soil diseases are synchronously achieved.

The application discloses a microbial agent for preventing and treating peanut root rot and application thereof, and belongs to the technical field of plant disease prevention and treatment. The microbial agent is prepared from raw materials including the following components in parts by weight: 1-5 parts of Trichoderma viride, 1-5 parts of Streptomyces microflavus, 5-8 parts of Paenibacillus polymyxa, 100-180 parts of traditional Chinese medicine extract, 0.5-2 parts of vitamin B1, 25-50 parts of sodium alginate, 10-20 parts of humic acid, 1-5 parts of zinc molybdate, 5-10 parts of copper sulfate, and 5-10 parts of ferrous phosphate. The microbial agent uses Trichoderma viride, Streptomyces microflavus and Paenibacillus polymyxa as biocontrol strains, uses traditional Chinese medicine extract, vitamin B1 and sodium alginate as auxiliary materials, and uses humic acid, zinc molybdate, copper sulfate and ferrous phosphate as nutrient supplements. The microbial agent can inhibit root rot fungi, reduce the incidence of root rot, and improve the peanut resistance and yield.

The application discloses a Streptomyces globisporus mutant gouC and an application thereof, and belongs to the technical field of genetic engineering. The Streptomyces globisporus mutant gouC is obtained by overexpressing a gouC gene, and the nucleotide sequence of the gouC gene is shown as SEQ ID NO. 3. The Streptomyces globisporus mutant gouC provided in the application can increase the production of gougerotin to 2.23 g / L. The application also provides an application of the Streptomyces globisporus mutant gouC in fermentation production of gougerotin. In addition, the fermentation product of the Streptomyces globisporus mutant gouC has significant bacteriostatic activity on Rhodotorula, and is suitable for application in the fields of agriculture and medicine.

The present application relates to the technical field of textiles, and particularly discloses an antibacterial fabric, a preparation method thereof and a clothes with rare earth magnetic particles. The preparation method of the antibacterial fabric is to blend and weave antibacterial fibers and high-elastic fibers to obtain the antibacterial fabric, wherein the antibacterial fibers are added with a mixed extract, which is obtained by fermenting polygonatum, mint leaves and cassava by fermenting lactobacillus fermentum, lactobacillus plantarum and pseudomonas fluorescens, and by fermenting streptomyces microflavus. The antibacterial fabric is obtained by using the preparation method of the antibacterial fabric. The clothes with rare earth magnetic particles is obtained by embedding high-energy rare earth magnetic particles on the clothes. The preparation method of the antibacterial fabric can effectively improve the antibacterial property, antibacterial stability and durability of the antibacterial fabric.