22 results about "Streptomyces microflavus" patented technology

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.

The invention discloses low-temperature salt-tolerant and alkali-tolerant streptomyces microflavus and application thereof, relates to the technical field of microorganisms, and is characterized in that the preservation number of the streptomyces microflavus is CCTCC (China Center for Type Culture Collection) M2023664. The streptomyces microflavus provided by the invention can well grow at the temperature of 10-30 DEG C. In a Congo red decoloration test, the ratio of the diameter (D) of a transparent circle to the diameter (d) of a bacterial colony is 3.6. The strain is applied to degradation of cellulose, has the advantages of degradation of cellulose at low temperature, high cellulase activity, salt resistance, alkali resistance and the like, and can be particularly used for cellulose degradation strain resources in low-temperature saline-alkali areas.

The invention relates to a preparation method of a salt-reducing bacterial fertilizer and an application of the salt-reducing bacterial fertilizer in agriculture, and the preparation method comprises the following steps: S1, mixing straws, mushroom cultivation wastes, animal dung and a culture medium matrix to obtain a biological waste-based culture medium; s2, respectively culturing bacillus licheniformis, bacillus subtilis, streptomyces microflavus, streptomyces jingyangensis and azotobacter chroococcum by adopting the biological waste material-based culture medium, so as to obtain a composite strain bacterial liquid; s3, mixing water, modified starch and starch, heating and gelatinizing, respectively mixing with the composite strain liquid, and stirring to obtain a starch-coated strain; and S4, mixing the starch-coated strain with nutrient substances to obtain the salt-reducing bacterial fertilizer. According to the invention, the possibility of mutual growth inhibition of different strains is reduced, the stability of the product is enhanced, the product can take effect for a long time, the functions of improving soil and promoting plant growth are played, and a new way is provided for farmland improvement and bacterial fertilizer efficiency improvement.

The present invention belongs to the technical field of maize disease control methods, and particularly relates to a biological bactericide for controlling maize ear rot, its preparation method and application. The biological bactericide comprises a water dispersible granule carrier mixture and a biocontrol bacteria culture solution, and the mass ratio of the water dispersible granule carrier mixture to the biocontrol bacteria culture solution is 2-3:1; the water dispersible granule carrier mixture comprises diatomaceous earth, chitosan and sucrose, and the mass ratio of diatomaceous earth, chitosan and sucrose is 1-2:1-2:1; the biocontrol bacteria culture solution is obtained by mixing fermentation broths of Bacillus subtilis, Streptomyces microflavus, Paenibacillus polymyxa and Bacillus velezensis. The biocontrol bactericide provided by the present invention has a very significant effect on controlling maize ear rot caused by Fusarium verticillioides, has the potential to be used as a new biological pesticide for controlling ear rot caused by Fusarium verticillioides in maize production, and has the value of further research.

The invention relates to the technical field of dandelion planting, and particularly discloses a high-yield disease-resistant planting method for dandelions. The dandelion planting method specifically comprises the following steps of planting land selection and treatment, sowing, field management and harvesting. According to the invention, during sowing, a self-made compound fertilizer, seaweed meal and a traditional Chinese medicine agent prepared from honeysuckle, houttuynia cordata and quisqualis indica are added, and the compound fertilizer, the seaweed meal and the traditional Chinese medicine agent are combined for use, so that more comprehensive nutrition is provided for the growth of dandelion; in the secondary fermentation process, compound microorganism bacteria formed by mixing pseudomonas aeruginosa, trichoderma harzianum and streptomyces microflavus according to a proportion are added, so that healthy growth of the dandelions is further promoted, and the dandelions planted through the method have the advantages of being high in yield, low in disease rate and good in quality.

The invention discloses a wormcast organic fertilizer and a preparation method thereof, relates to the technical field of organic fertilizer preparation, and aims to solve the problems of nutrient imbalance, insufficient decomposition, poor slow release performance and the like of the existing wormcast fertilizer. The method comprises the following steps: by taking agricultural wastes such as straw powder, peanut shell powder and the like, cow dung and wormcast as raw materials, inoculating a bacillus subtilis microbial inoculum containing alginate oligosaccharide, an aspergillus sydowii microbial inoculum and a streptomyces microflavus microbial inoculum containing mannitol, performing segmented fermentation, adding a multifunctional adhesive, and granulating, thereby obtaining the feed additive. The product realizes balanced nutrients and full decomposition, has good slow release performance and high particle strength, efficiently utilizes agricultural wastes, and improves the practicability and environmental protection value of the fertilizer.

The invention relates to a method for purifying organic sewage according to strain decomposition and adaptive sequential release. According to the method, bacillus subtilis, bacillus cereus, streptomyces microflavus and rhodopseudomonas palustris which have a mutual growth promotion effect are firstly released in organic sewage, macromolecular substances such as protein, ester, fiber and starch are firstly decomposed, and rhodotorula mucilaginosa, faecalis coccus and clostridium butyricum are released by adopting a microcapsule slow release method; furthermore, small molecules such as amino acid, glucose, fatty acid and the like are utilized, so that the organic pollutants are finally degraded into small-molecule organic acid, carbon dioxide and water. The method disclosed by the invention is suitable for reducing COD and BOD levels of food industrial wastewater, and has the characteristics of low cost and convenience in manufacturing and use.

The application relates to a biological saline-alkali soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. In order to solve the problems of low activity, single function and poor aggregation promotion effect of an existing biological conditioner in a low-temperature saline-alkali environment of black soil in a cold region, the application provides a biological saline-alkali soil conditioner which comprises a multifunctional composite bacterial system Z2 composed of bacillus velezensis PZ-3, bacillus cereus K1, streptomyces microflavus CLSD-1 and pseudomonas alcaliphila WP9, and a carrier matrix auxiliary material composed of chitosan, polyacrylamide and biogas residue. The biological saline-alkali soil conditioner provided by the application has multiple functions of low-temperature saline-alkali resistance, yield increase, straw degradation and aggregation body formation, can effectively reduce the soil pH and bulk density, improve the aggregation body proportion and moisture content, significantly promote crop growth and increase yield, is suitable for the improvement of black soil and low-temperature saline-alkali soil in the northeast cold region, and is environment-friendly and free of chemical residues.

The present application relates to a kind of microbial slow-release bacterial fertilizer and its preparation method and application, belong to microbial bacterial fertilizer technical field.The microbial slow-release bacterial fertilizer described in the present application contains composite microorganism 15-18 parts, soybean root powder 8-10 parts, β-cyclodextrin 20-23 parts, grasswood ash 15-20 parts, humic acid 5-8 parts, tea saponin 5-8 parts, potassium fulvate 13-15 parts, carboxymethyl chitosan 18-20 parts by weight;Wherein composite microorganism contains round brown nitrogen-fixing bacteria, bacillus subtilis, streptomyces microflavus.The microbial composite bacterial fertilizer of the present application can effectively reduce soil acidity, improve soil structure, release nutrient components, increase soil fertility, be conducive to promoting crop growth and improving crop yield.

The invention relates to application of a streptomyces albidoflavus strain SaTC2601 to promotion of straw conversion of protaetia brevitarsis. The streptomyces albidoflavus is found to be capable of promoting the straw conversion rate of the protaetia brevitarsis larvae and promoting the increase of the protaetia brevitarsis larvae after being used for treating the straws, so that not only is the absorption of straw non-point source pollution facilitated, but also the protein yield of the protaetia brevitarsis larvae can be increased by increasing the weight of the protaetia brevitarsis larvae; therefore, a channel which is more beneficial to guaranteeing the national grain safety is provided.

The application discloses a kind of biological inoculant for preventing and treating wheat scab and a preparation method and application thereof, belong to plant disease control technical field.The microbial inoculant is prepared from the following raw materials by weight percentage: Trichoderma harzianum 5-10 parts, Streptomyces microflavus 2-5 parts, Bacillus subtilis 2-5 parts, soybean meal 75-90 parts, vitamin B1 0.1-0.5 parts, potassium fulvate 2-5 parts and ferrous phosphate 2-5 parts.The microbial inoculant of the application uses Trichoderma harzianum, Streptomyces microflavus and Bacillus subtilis as biocontrol strains, which has inhibitory effect on Fusarium asiaticum.The wheat scab control scheme of the application is environmentally friendly and sustainable.The application enhances the resistance and biodiversity of crops, reduces the dependence on chemical pesticides and promotes the balance of agricultural ecology.

The present invention provides a compound microbial inoculant and its application, belonging to the technical field of agricultural microorganisms. Specifically, it is a compound microbial inoculant, which comprises the following components: Streptomyces microflavus, Bacillus firmus, Trichoderma longibrachiatum, welan gum, and fucoidan oligosaccharide. The compound microbial inoculant provided by the present invention can effectively colonize in saline-alkali soil, and is more conducive to colonizing around the roots, especially the colonization effect around the roots of gramineous plants is better; and it can effectively increase the root proliferation of crops in saline-alkali soil, especially the root proliferation effect of gramineous crops is better, and effectively reduces the dead seedling rate of corn seedlings when planting corn in saline-alkali soil.

The invention provides a three-river-source moderately-degraded alpine meadow microbial complex microbial inoculant synergistic reseeding rapid recovery method and application, and belongs to the technical field of soil remediation. The invention provides a microbial complex microbial inoculant and application of synergistic reseeding of the microbial complex microbial inoculant in degraded meadow repair. The microbial complex microbial inoculants comprise rhizopus intro-rhizosporium and streptomyces microflavus; the reseeded plant seeds comprise seeds of gramineous plants, leguminous plants and miscellaneous grass plants. By introducing two dominant bacteria, the utilization rate of plants to soil resources is improved, the plants have higher environmental stress coping capacity, and by means of the synergistic reseeding technology, the vegetation community composition and the species important value are improved. According to the method disclosed by the invention, the microbial complex microbial inoculants are applied to the restoration of the moderately degraded alpine meadow in the Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-Yangtze-

The invention discloses low-temperature glucose-tolerant beta-glucosidase and application thereof, and relates to the field of enzyme engineering. The beta-glucosidase is separated from Streptomyces microflavus CLSD-1 and belongs to a GH1 family, and the amino acid sequence of the beta-glucosidase is shown as SEQ ID No: 1 or SEQ ID No: 2. The application of the beta-glucosidase is that the beta-glucosidase is applied to simultaneous saccharification and fermentation production of ethanol from cellulose. The beta-glucosidase (Bgl2350 and Bgl5676) provided by the invention has the characteristics of low temperature and high activity, the optimal reaction temperatures are respectively 40 DEG C and 30 DEG C, and both the beta-glucosidase and the Bgl5676 have excellent glucose and xylose tolerance and can tolerate 600mM glucose and 2M xylose environments. Wherein the enzyme activity of the Bgl2350 is relatively high under a low-temperature condition; the Bgl5676 shows the stability and the salt tolerance which are obviously superior to those of the traditional GH1 family beta-glucosidase. Compared with the commercially available Novozymes Cellic CTec3 commercial enzyme, the Bgl2350 and the Bgl5676 show more prominent application potential in the field of low-temperature cellulose saccharification, and can partially replace the beta-glucosidase component in the commercial enzyme in practical application.

The application discloses a micro-white Streptomyces avermitilis strain SaTC2601 and application thereof, and belongs to the field of biological control. The micro-white Streptomyces avermitilis strain SaTC2601 is isolated from the excrement of white star flower chafer (WSFC) larvae, and has a preservation number of CGMCC No.33741. The strain has a good prevention and treatment effect on peanut white thread blight caused by Sclerotium rolfsii on peanut roots, and also has a prevention and treatment effect on soil-borne pathogenic bacteria such as Fusarium graminearum, Fusarium solani, Fusarium moniliforme, Fusarium oxysporum, Rhizoctonia solani and Streptomyces scabiei. Further, the strain SaTC2601 also has a good growth promotion effect. The strain becomes a valuable resource for new drug development and agricultural disease prevention and control, and is expected to play a huge role in soil disease prevention and control.

The invention discloses a compound bacterial fertilizer for improving drought resistance of rape and application of the compound bacterial fertilizer, and belongs to the technical field of agricultural planting. The compound bacterial fertilizer comprises 40 wt%-45 wt% of functional components and 55 wt%-60 wt% of compound fertilizer, the functional component is prepared from the following raw materials in parts by weight: 0.5 to 2 parts of paenibacillus polymyxa, 0.5 to 2 parts of streptomyces microflavus, 2 to 5 parts of trichoderma harzianum, 0.05 to 0.2 part of brassinosterol, 0.3 to 0.5 part of alginic acid and 4 to 6 parts of humic acid. The compound bacterial fertilizer provided by the invention has a remarkable effect on improving the drought resistance of the rape, and can relieve rape cell damage caused by drought, maintain a photosynthetic function, promote dry matter accumulation and improve the drought resistance and yield of the rape. The method has important practical significance for solving the problem of rape drought stress and promoting green and sustainable development of the rape industry.

The invention provides a culture medium and a preparation method and application thereof, and belongs to the technical field of tailing utilization. The invention provides a culture medium which comprises the following components in parts by mass: 1-7 parts of an algae-bacterium symbiont, 1-12 parts of modified illite tailings and 1-22 parts of sheep manure, the algae-bacterium symbiont comprises chlorella and streptomyces microflavus. The culture medium provided by the invention meets the requirements of the standard NY / 2118-2012 on a vegetable seedling culture medium, and has the effects of promoting plant growth and improving plant stress resistance while improving the physical and chemical properties of soil. The culture medium provided by the invention has the advantages of environmental protection, sustainable utilization and the like, accords with the development trend of modern agriculture, and provides a new direction for resource utilization of the illite tailings; efficient cyclic utilization of mine tailings and agricultural wastes is also realized, and an innovative path is provided for green development and resource utilization of agriculture.

The invention discloses a bio-organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of organic fertilizers. The preparation method comprises the following steps: respectively pretreating apple pomace, straw, cow dung and sheep manure, and mixing to obtain a mixture; bacillus subtilis, Streptomyces microflavus, Streptomyces griseus and Trichoderma harzianum are respectively cultured and then are mixed to obtain a mixed bacterial liquid. The preparation method comprises the following steps: adding a mixed bacterial solution into a mixture for high-temperature aerobic fermentation, then adding chitin for medium-temperature facultative fermentation, and finally adding selenized potassium humate and ground phosphate rock for anaerobic fermentation, thereby finally obtaining the biological organic fertilizer. The biological organic fertilizer with the characteristics of high organic matter content, high antibacterial activity and slow release of nutrients is produced within 30 days by virtue of pretreatment optimization of the raw materials, time sequence regulation and control of the complex microbial inoculants and accurate addition of synergistic substances, and the targets of waste recycling and ecological prevention and control of soil diseases are synchronously achieved.

The application discloses a microbial agent for preventing and treating peanut root rot and application thereof, and belongs to the technical field of plant disease prevention and treatment. The microbial agent is prepared from raw materials including the following components in parts by weight: 1-5 parts of Trichoderma viride, 1-5 parts of Streptomyces microflavus, 5-8 parts of Paenibacillus polymyxa, 100-180 parts of traditional Chinese medicine extract, 0.5-2 parts of vitamin B1, 25-50 parts of sodium alginate, 10-20 parts of humic acid, 1-5 parts of zinc molybdate, 5-10 parts of copper sulfate, and 5-10 parts of ferrous phosphate. The microbial agent uses Trichoderma viride, Streptomyces microflavus and Paenibacillus polymyxa as biocontrol strains, uses traditional Chinese medicine extract, vitamin B1 and sodium alginate as auxiliary materials, and uses humic acid, zinc molybdate, copper sulfate and ferrous phosphate as nutrient supplements. The microbial agent can inhibit root rot fungi, reduce the incidence of root rot, and improve the peanut resistance and yield.

The present application discloses a tomato package fertilizer for resisting continuous cropping diseases and a method for applying the same, belonging to the technical field of fertilizers. The tomato package fertilizer for resisting continuous cropping diseases in the present application includes a solid base fertilizer, a liquid root-strengthening fertilizer, a liquid seedling-strengthening fertilizer, and a liquid flower and fruit fertilizer; the base fertilizer is composed of food waste fermentation material and compound microbial agent A, and the compound microbial agent A contains Bacillus velezensis and Streptomyces microflavus; the root-strengthening fertilizer contains kitchen waste fermentation liquid, lysine solution, mineral source humic acid, polyacrylamide, seaweed polysaccharide, and compound microbial agent B; the effective components of the seedling-strengthening fertilizer are, calculated as pure nutrients: N 150-200 g / L, P2O5 100-120 g / L, K2O 150-200 g / L, organic matter 45-55 g / L, γ-polyglutamic acid 2-4 g / L; the effective components of the flower and fruit fertilizer are, calculated as pure nutrients: N 140-160 g / L, P2O5 60-80 g / L, K2O 200-220 g / L, Zn 0.5-0.7 g / L, B 0.2-0.4 g / L, organic matter 120-125 g / L, enzymatically hydrolyzed amino acid 10-12 g / L. The package fertilizer in the present application can quickly meet the nutrient requirements of tomatoes at different growth and development stages, reduce fertilizer waste while ensuring the healthy growth of tomatoes, effectively prevent the occurrence of continuous cropping diseases, and greatly improve the crop quality and yield.

The application discloses a Streptomyces globisporus mutant gouC and an application thereof, and belongs to the technical field of genetic engineering. The Streptomyces globisporus mutant gouC is obtained by overexpressing a gouC gene, and the nucleotide sequence of the gouC gene is shown as SEQ ID NO. 3. The Streptomyces globisporus mutant gouC provided in the application can increase the production of gougerotin to 2.23 g / L. The application also provides an application of the Streptomyces globisporus mutant gouC in fermentation production of gougerotin. In addition, the fermentation product of the Streptomyces globisporus mutant gouC has significant bacteriostatic activity on Rhodotorula, and is suitable for application in the fields of agriculture and medicine.

The present application relates to the technical field of textiles, and particularly discloses an antibacterial fabric, a preparation method thereof and a clothes with rare earth magnetic particles. The preparation method of the antibacterial fabric is to blend and weave antibacterial fibers and high-elastic fibers to obtain the antibacterial fabric, wherein the antibacterial fibers are added with a mixed extract, which is obtained by fermenting polygonatum, mint leaves and cassava by fermenting lactobacillus fermentum, lactobacillus plantarum and pseudomonas fluorescens, and by fermenting streptomyces microflavus. The antibacterial fabric is obtained by using the preparation method of the antibacterial fabric. The clothes with rare earth magnetic particles is obtained by embedding high-energy rare earth magnetic particles on the clothes. The preparation method of the antibacterial fabric can effectively improve the antibacterial property, antibacterial stability and durability of the antibacterial fabric.