Preparation method of salt-reducing bacterial fertilizer and application of salt-reducing bacterial fertilizer in agriculture

Abstract

The invention relates to a preparation method of a salt-reducing bacterial fertilizer and an application of the salt-reducing bacterial fertilizer in agriculture, and the preparation method comprises the following steps: S1, mixing straws, mushroom cultivation wastes, animal dung and a culture medium matrix to obtain a biological waste-based culture medium; s2, respectively culturing bacillus licheniformis, bacillus subtilis, streptomyces microflavus, streptomyces jingyangensis and azotobacter chroococcum by adopting the biological waste material-based culture medium, so as to obtain a composite strain bacterial liquid; s3, mixing water, modified starch and starch, heating and gelatinizing, respectively mixing with the composite strain liquid, and stirring to obtain a starch-coated strain; and S4, mixing the starch-coated strain with nutrient substances to obtain the salt-reducing bacterial fertilizer. According to the invention, the possibility of mutual growth inhibition of different strains is reduced, the stability of the product is enhanced, the product can take effect for a long time, the functions of improving soil and promoting plant growth are played, and a new way is provided for farmland improvement and bacterial fertilizer efficiency improvement.

Description

Technical Field

[0001] The present invention relates to the technical field of agriculture, and particularly to a preparation method of a salt-reducing bacteria fertilizer and its application in agriculture. Background Art

[0002] Traditional agricultural production usually applies single chemical fertilizers, which are compounded and applied with auxiliaries such as herbicides and insecticides. However, long-term application of chemical fertilizers seriously damages the soil structure, reduces soil fertility, and more seriously, leads to soil salinization and pollution of the environment. Biological improvement technologies have attracted attention due to their advantages such as environmental friendliness and sustainability. Using microorganisms as components to develop new biomass fertilizers has become a research hotspot in this field.

[0003] Compound bacterial fertilizers are usually compounded with various functional microorganisms such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, and salt-tolerant bacteria. Through the synergistic cooperation of the bacterial communities, they secrete substances such as organic acids and polysaccharides to chelate sodium ions and reduce the salt content; they can also synthesize specific amino acids, proteins and other substances to enhance the salt tolerance of plants, thereby reducing soil salinity through microbial metabolic activities and achieving effects such as improving the crop growth environment, increasing nutrient utilization efficiency, and promoting crop yield. Existing technologies such as CN118125884A - A Compound Bacterial Fertilizer, CN112154759A - An Environmentally Friendly Compound Biological Bacterial Fertilizer, CN103992191A - A Composite Biological Bacterial Fertilizer and other technical solutions have all achieved the functions of improving soil structure, enhancing soil activity, and promoting crop growth by using microorganisms and nutrients as the main components.

[0004] However, different strains in the compound bacterial fertilizer may interfere with each other due to nutrient competition, metabolite inhibition, or environmental adaptability differences, forming an antagonistic effect. For example, some strains can secrete antibiotics, which will inhibit the activity of other strains; fast-growing strains may seize resources and cause the inactivation of weak bacterial communities. This antagonistic effect will reduce the synergistic effect of the functional bacterial communities in the compound bacterial fertilizer and affect its effect of improving the soil and promoting crop growth. And there are few solutions to this problem in the existing technologies.

[0005] In summary, it is urgent to develop a new technical solution to overcome the defects existing in the existing technologies. Summary of the Invention

[0006] Based on this, the present invention provides a preparation method of a salt-reducing bacteria fertilizer and its application in agriculture. The present invention uses agricultural wastes such as straw, mushroom cultivation waste, and livestock manure as a culture medium to culture Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis, and Azotobacter chroococcum to obtain a composite strain bacterial liquid, and then coats it with modified starch, reducing the possibility of mutual inhibition of growth among different strains, and at the same time enhancing the product stability, enabling it to take effect more durably and play the functions of improving soil and promoting plant growth, providing a new way for arable land improvement and improving the efficiency of bacterial fertilizer.

[0007] The purpose of the present invention is to provide a preparation method of a salt-reducing bacteria fertilizer, and the preparation method of the salt-reducing bacteria fertilizer includes the following steps:

[0008] S1. Mix straw, mushroom cultivation waste, livestock manure, and a culture medium matrix to obtain a biological waste-based culture medium;

[0009] S2. Use the biological waste-based culture medium to separately culture Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis, and Azotobacter chroococcum to obtain a composite strain bacterial liquid;

[0010] S3. Mix water, modified starch, and starch, heat and gelatinize them, and then separately mix them with the composite strain bacterial liquid, and stir to obtain starch-coated strains;

[0011] S4. Mix the starch-coated strains with nutrients to obtain a salt-reducing bacteria fertilizer;

[0012] Among them, the modified starch is obtained by the reaction of epoxy polypropylene glycol and starch.

[0013] Furthermore, it also includes:

[0014] S2.5. Adsorb the composite strain bacterial liquid with diatomaceous earth respectively.

[0015] Furthermore, the preparation method of the modified starch includes the following steps:

[0016] Mix starch and an alkali catalyst, heat and react, then add epoxy polypropylene glycol, and continue to react to obtain modified starch.

[0017] Furthermore, the mass ratio of the starch to the epoxy polypropylene glycol is 2:(1 - 4).

[0018] Furthermore, the temperature of the heat reaction is 60 - 80 °C, the time is 1 - 2 h, and the time of the continued reaction is 3 - 7 h.

[0019] Furthermore, the concentration of the composite strain bacterial liquid is 1 - 9×10 8 cfu / mL.

[0020] Furthermore, the mass ratio of the diatomaceous earth to the composite bacterial strain liquid is 1:(1 - 3).

[0021] Furthermore, the mass ratio of the modified starch to the starch is (0.8 - 1.2):1.

[0022] Furthermore, the nutrient substances include nitrogen fertilizer, phosphate fertilizer and potassium fertilizer.

[0023] Furthermore, the nutrient substances include urea, diammonium hydrogen phosphate and potassium sulfate.

[0024] Furthermore, in step S3, sodium hexametaphosphate as a cross-linking agent is added.

[0025] Furthermore, the epoxy group polypropylene glycol is polypropylene glycol diglycidyl ether.

[0026] Furthermore, the mass ratio of the starch-coated bacterial strain, urea, diammonium hydrogen phosphate and potassium sulfate is (3 - 7):(3 - 6):(4 - 8):(3 - 6).

[0027] Furthermore, the dosage ratio of Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis and Azotobacter chroococcum is (1 - 2):(1 - 2):(0.5 - 1):(0.1 - 0.3):(0.5 - 1).

[0028] Another object of the present invention is to provide an application of the preparation method of the above-mentioned salt-reducing bacterial fertilizer in agriculture.

[0029] The present invention has the following beneficial effects:

[0030] The salt-reducing bacterial fertilizer provided by the present invention uses agricultural wastes such as straw, mushroom cultivation waste and livestock manure as culture medium components, cultures five bacterial strains of Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis and Azotobacter chroococcum to obtain a bacterial liquid, then adsorbs it with diatomaceous earth, and then coats it with modified starch to obtain a starch-coated bacterial strain (or directly coats the bacterial liquid with modified starch), and after compounding with nutrient substances, a salt-reducing bacterial fertilizer is obtained.

[0031] Agricultural production wastes are usually difficult to be properly treated and have a greater impact on the ecological environment and daily life. The present invention uses agricultural wastes containing rich nutrients such as cellulose, starch, protein and amino acids as culture media, which can not only serve as a nutrient source for the growth of microorganisms, but also serve as an organic fertilizer to promote plant growth in the subsequent crop cultivation process. It serves multiple purposes, realizes the recycling of waste, is beneficial to the sustainable development of agriculture, and provides a new idea for the research and production of compound bacterial fertilizers.

[0032] The modified starch of the present invention is obtained by first generating an oxygen anion from the hydroxyl group of starch under the catalysis of an alkali, and then further reacting with the epoxy group of polypropylene glycol diglycidyl ether. On the one hand, polypropylene glycol diglycidyl ether can play a cross-linking role, which is beneficial to improving the coating and film-forming ability of the modified starch and has a better wrapping effect on the bacterial strains. On the other hand, the propoxy chain segment has certain hydrophobicity, which improves the stability of the modified starch, extends the storage time of the bacterial fertilizer, ensures the activity of the bacterial strains, and enables the product to take effect persistently and stably. More importantly, after coating the bacterial strains, the competition or antagonistic effect between different bacterial strains (for example, Streptomyces microflavus and Streptomyces jingyangensis may produce hormones and antibiotics) is reduced, ensuring the stable growth of the composite bacterial strains, so as to play a synergistic role, jointly improve the soil salinization degree, improve the soil structure, and increase the crop yield, achieving multiple effects. Detailed Embodiments

[0033] In order to more clearly illustrate the technical solutions of the present invention, the following examples are listed. The raw materials, reactions, and post-treatment means appearing in the examples are common raw materials on the market and technical means well-known to those skilled in the art, unless otherwise specified.

[0034] The terms "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects under certain circumstances. However, in the same or other circumstances, other embodiments may also be preferred. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not available, nor is it intended to exclude other embodiments from the scope of the present invention.

[0035] It should be understood that, except in any operating example or otherwise indicated, all numbers representing the amounts of ingredients or otherwise used in the specification and claims should be understood to be modified in all instances by the term "about". Therefore, unless otherwise indicated, the numerical parameters set forth in the following specification and appended claims are approximations that vary depending upon the desired properties sought to be obtained by the present invention.

[0036] Bacillus licheniformis (GDMCC NO.1.11), Bacillus subtilis (GDMCC NO.1.131), Streptomyces microflavus (GDMCC NO.4.26), Streptomyces jingyangensis (GDMCC NO.4.92), and Azotobacter chroococcum (GDMCC NO.1.272) in the embodiments of the present invention are all purchased from the Guangdong Provincial Culture Collection of Microorganisms.

[0037] The straw (corn straw), mushroom cultivation waste (mushroom residue), and livestock manure (cow dung) in the embodiments of the present invention are all purchased from the local market and farms, and are sterilized before use.

[0038] The culture medium matrix in the embodiments of the present invention consists of the following components: 3.0 g of beef extract, 5.0 g of peptone, 10.0 g of glucose, 1.0 g of sodium chloride, 0.5 g of potassium dihydrogen phosphate, 1.0 L of distilled water, and the pH is adjusted to 7.0.

[0039] The starch in the embodiments of the present invention is corn starch.

[0040] The epoxy group polypropylene glycol in the embodiments of the present invention is polypropylene glycol diglycidyl ether, purchased from Sigma - Aldrich, M n ≈380.

[0041] The preparation method of the modified starch in the embodiments of the present invention includes the following steps:

[0042] Mix 20 parts of isopropanol and 15 parts of water as a solvent, add 10 parts of starch, then stir and add 0.9 part of sodium hydroxide. After reacting at 75 °C for 1 h, add 10 parts of epoxy group polypropylene glycol and continue to react for 6 h. After cooling, adjust the pH to 7.0 with glacial acetic acid, and obtain the modified starch after centrifugation, washing, and drying.

[0043] All "parts" in the embodiments of the present invention refer to parts by mass.

[0044] Example 1

[0045] A preparation method of a salt - reducing bacteria bacterial fertilizer, the preparation method of the salt - reducing bacteria bacterial fertilizer includes the following steps:

[0046] S1. Mix 3 parts of straw, 2 parts of mushroom cultivation waste, and 0.2 part of livestock and poultry manure, dry, crush through a 50 - mesh sieve, and mix with 10 parts of the culture medium matrix to obtain a biological waste - based culture medium;

[0047] S2. Add the activated Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis, and Azotobacter chroococcum into the biological waste - based culture medium for enlarged cultivation, cultivate at 30 °C for 48 h, and adjust the bacterial liquid concentration to 1×10 8 cfu / mL to obtain Bacillus licheniformis bacterial liquid, Bacillus subtilis bacterial liquid, Streptomyces microflavus bacterial liquid, Streptomyces jingyangensis bacterial liquid, and Azotobacter chroococcum bacterial liquid;

[0048] S2.5. Mix diatomaceous earth and Bacillus licheniformis bacterial liquid with a mass ratio of 1:1, stir evenly, stand for 2 h, and dry to obtain intermediate product 1;

[0049] Mix diatomaceous earth and Bacillus subtilis bacterial liquid with a mass ratio of 1:1, stir evenly, stand for 2 h, and dry to obtain intermediate product 2;

[0050] Mix diatomaceous earth and Streptomyces microflavus bacterial liquid with a mass ratio of 1:1, stir evenly, stand for 2 h, and dry to obtain intermediate product 3;

[0051] Mix diatomaceous earth and Streptomyces jingyangensis bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 4;

[0052] Mix diatomaceous earth and Azotobacter chroococcum bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 5;

[0053] S3. Add 40 g of modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 1 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 1;

[0054] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 2 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 2;

[0055] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 3 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 3;

[0056] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 4 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 4;

[0057] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 5 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 5;

[0058] S4. Mix 1 part of starch-coated strain 1, 1 part of starch-coated strain 2, 1 part of starch-coated strain 3, 0.2 part of starch-coated strain 4, 1 part of starch-coated strain 5, 3 parts of urea, 6 parts of diammonium hydrogen phosphate, and 3 parts of potassium sulfate to obtain the salt-reducing bacterial fertilizer.

[0059] Example 2

[0060] A preparation method of a salt-reducing bacterial fertilizer, the preparation method of the salt-reducing bacterial fertilizer includes the following steps:

[0061] S1. Mix 3 parts of straw, 2 parts of mushroom cultivation waste, and 0.2 parts of livestock manure. After drying, crush them through a 50-mesh sieve and mix with 10 parts of culture medium substrate to obtain a biological waste-based culture medium;

[0062] S2. Add the activated Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis, and Azotobacter chroococcum to the biological waste-based culture medium for enlarged cultivation. Cultivate at 30 °C for 48 h and adjust the bacterial liquid concentration to 1×10 8 cfu / mL to obtain Bacillus licheniformis bacterial liquid, Bacillus subtilis bacterial liquid, Streptomyces microflavus bacterial liquid, Streptomyces jingyangensis bacterial liquid, and Azotobacter chroococcum bacterial liquid;

[0063] S2.5. Mix diatomaceous earth and Bacillus licheniformis bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 1;

[0064] Mix diatomaceous earth and Bacillus subtilis bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 2;

[0065] Mix diatomaceous earth and Streptomyces microflavus bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 3;

[0066] Mix diatomaceous earth and Streptomyces jingyangensis bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 4;

[0067] Mix diatomaceous earth and Azotobacter chroococcum bacterial liquid with a mass ratio of 1:1, stir evenly, let stand for 2 h, and dry to obtain intermediate product 5;

[0068] S3. Add 40 g of modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 1 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 1;

[0069] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 2 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 2;

[0070] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 3 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and dry to obtain starch-coated strain 3;

[0071] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 4 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and obtain starch-coated strain 4 after drying;

[0072] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add a mixture of 20 g of intermediate product 5 and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and obtain starch-coated strain 5 after drying;

[0073] S4. Mix 1 part of starch-coated strain 1, 2 parts of starch-coated strain 2, 0.5 part of starch-coated strain 3, 0.2 part of starch-coated strain 4, 1 part of starch-coated strain 5, 3 parts of urea, 6 parts of diammonium hydrogen phosphate, and 3 parts of potassium sulfate to obtain a salt-reducing bacteria fertilizer.

[0074] Example 3

[0075] A preparation method of a salt-reducing bacteria fertilizer, the preparation method of the salt-reducing bacteria fertilizer includes the following steps:

[0076] S1. Mix 3 parts of straw, 2 parts of mushroom cultivation waste, and 0.2 part of livestock and poultry manure, dry, crush through a 50-mesh sieve, and mix with 10 parts of culture medium matrix to obtain a biological waste-based culture medium;

[0077] S2. Respectively add the activated Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis, and Azotobacter chroococcum to the biological waste-based culture medium for subculture, culture at 30 °C for 48 h, and adjust the bacterial liquid concentration to 1×10 8 cfu / mL to obtain Bacillus licheniformis bacterial liquid, Bacillus subtilis bacterial liquid, Streptomyces microflavus bacterial liquid, Streptomyces jingyangensis bacterial liquid, and Azotobacter chroococcum bacterial liquid;

[0078] S3. Add 40 g of modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add 15 mL of Bacillus licheniformis bacterial liquid, let stand for 12 h, and obtain starch-coated strain 1 after drying;

[0079] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add 15 mL of Bacillus subtilis bacterial liquid, let stand for 12 h, and obtain starch-coated strain 2 after drying;

[0080] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min. After cooling, stir and add 15 mL of Streptomyces microflavus bacterial liquid, let stand for 12 h, and obtain starch-coated strain 3 after drying;

[0081] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min, after cooling, stir in 15 mL of Streptomyces jingyangensis bacterial liquid, let stand for 12 h, and obtain starch-coated strain 4 after drying;

[0082] Add 40 g of the modified starch and 40 g of starch to 800 g of water, stir at 80 °C for 5 min, after cooling, stir in 15 mL of Azotobacter chroococcum bacterial liquid, let stand for 12 h, and obtain starch-coated strain 5 after drying;

[0083] S4. Mix 1 part of starch-coated strain 1, 1 part of starch-coated strain 2, 1 part of starch-coated strain 3, 0.2 part of starch-coated strain 4, 1 part of starch-coated strain 5, 3 parts of urea, 6 parts of diammonium hydrogen phosphate, and 3 parts of potassium sulfate to obtain a salt-reducing bacterial fertilizer.

[0084] Comparative Example 1

[0085] The difference between this comparative example and Example 1 is that the modified starch is replaced with unmodified starch, and other components and preparation methods are the same as those in Example 1.

[0086] Comparative Example 2

[0087] Steps S1 and S2 of this comparative example are the same as those in Example 1, and step S3 is as follows:

[0088] Mix Bacillus licheniformis bacterial liquid, Bacillus subtilis bacterial liquid, Streptomyces microflavus bacterial liquid, Streptomyces jingyangensis bacterial liquid, Azotobacter chroococcum bacterial liquid, and diatomite with a mass ratio of 1:1:1:0.2:1:4.2, stir evenly, let stand for 2 h, and obtain an intermediate product after drying;

[0089] Add 80 g of starch to 800 g of water, stir at 80 °C for 5 min, after cooling, stir in a mixture of 20 g of the intermediate product and 80 g of water, then add 0.6 g of sodium hexametaphosphate, let stand for 12 h, and obtain starch-coated strain after drying;

[0090] Mix 4 parts of the starch-coated strain, 3 parts of urea, 6 parts of diammonium hydrogen phosphate, and 3 parts of potassium sulfate to obtain a salt-reducing bacterial fertilizer.

[0091] Test Example 1

[0092] Perform performance tests on the salt-reducing bacterial fertilizers prepared in the examples and comparative examples.

[0093] Test method:

[0094] Take 5 kg of planting soil and add it to flowerpots, a total of 15 flowerpots, divided into 5 groups, with 3 flowerpots in each group. Add the fertilizer samples prepared in Examples 1-3 or Comparative Examples 1-2 to each group at 100 g / pot, and mix evenly.

[0095] After germinating the variegated lettuce seeds and raising seedlings, select seedlings with the same growth potential (after growing the first true leaf), transplant them into pots at a rate of 3 plants per pot, and culture them at a constant temperature of 25°C for 30 days. During this period, the management of the lettuce follows the agricultural timing operations.

[0096] The average weight of the aerial part of the lettuce after cultivation was measured.

[0097] The test results are shown in Table 1.

[0098] Table 1 Test results

[0099] Project Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Lettuce weight / g 137.6 140.8 131.9 129.3 119.7

[0100] According to Table 1, it can be concluded that the salt-reducing bacteria fertilizer of the embodiment of the present invention has a good promoting effect on the growth of lettuce, while the comparative example 1-2, which is coated with conventional starch or directly mixes different bacterial solutions, is difficult to obtain an ideal synergistic effect due to the reduced stability and antagonism between bacterial species, and has a poor growth-promoting effect on lettuce.

[0101] Test Example 2

[0102] Salt reduction performance test.

[0103] Test method:

[0104] The starch-coated strains 1-5 obtained in step S3 of Example 1 were mixed in a mass ratio of 1:1:1:0.2:1 to obtain a bacterial agent, and then 20 g of the bacterial agent was added to 5 kg of local salinized soil (water content of 20 wt%), placed in a flower pot, and treated at a constant temperature of 30° C. for 30 days.

[0105] Soil and deionized water were taken before and after treatment, mixed and extracted in a mass ratio of 1:5, and the conductivity of the solution was measured.

[0106] The test results are shown in Table 2.

[0107] Table 2 Test results

[0108]

[0109] It can be seen from Table 2 that adding the starch-coated bacteria in the embodiment of the present invention to salinized soil for cultivation can effectively reduce the soil conductivity, proving that the soluble salt content in the soil is greatly reduced.

[0110] Therefore, the present invention can significantly improve the salinization and salinization defects of the soil, and can effectively promote the growth of crops, and has good application prospects.

[0111] It is obvious to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, in any regard, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Accordingly, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention.

[0112] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A preparation method of a salt-reducing bacteria fertilizer, characterized in that, The preparation method of the salt-reducing bacteria fertilizer comprises the following steps: S1. Mix straw, mushroom cultivation waste, livestock and poultry manure and a culture medium matrix to obtain a biological waste-based culture medium; S2. Use the biological waste-based culture medium to culture Bacillus licheniformis, Bacillus subtilis, Streptomyces microflavus, Streptomyces jingyangensis and Azotobacter chroococcum respectively to obtain a composite strain bacterial liquid; S3. Mix water, modified starch and starch, heat and gelatinize them, and then mix them with the composite strain bacterial liquid respectively, and stir to obtain starch-coated strains; S4. Mix the starch-coated strains with nutrients to obtain the salt-reducing bacteria fertilizer; Wherein, the modified starch is obtained by the reaction of epoxy polypropylene glycol and starch.

2. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 1, wherein, It also includes: S2.

5. Adsorb the composite strain bacterial liquid with diatomite respectively.

3. The preparation method of the salt-reducing bacteria fertilizer according to claim 1, characterized in that, The preparation method of the modified starch comprises the following steps: Mix starch and an alkali catalyst, heat and react, then add epoxy polypropylene glycol, and continue to react to obtain modified starch.

4. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 3, characterized in that, The mass ratio of the starch to the epoxy polypropylene glycol is 2:(1-4).

5. The preparation method of the salt-reducing bacteria microbial fertilizer according to claim 3, wherein The temperature of the heat reaction is 60-80°C, the time is 1-2h, and the time of the continued reaction is 3-7h.

6. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 1, characterized in that, The concentration of the composite bacterial strain solution is 1-9×10 8 cfu / mL.

7. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 2, wherein, The mass ratio of the diatomite to the composite strain bacterial liquid is 1:(1-3).

8. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 1, characterized in that, The mass ratio of the modified starch to the starch is (0.8-1.2):

1.

9. The preparation method of the salt-reducing bacteria bacterial fertilizer according to claim 1, characterized in that, The nutrients include nitrogen fertilizer, phosphate fertilizer and potassium fertilizer.

10. Application of the preparation method of the salt-reducing bacteria fertilizer according to any one of claims 1-9 in agriculture.

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