Multivalent inactivated vaccine against photobacterium damselae subsp. damselae and preparation method thereof

A technology of photobacteria and multivalent vaccines, which is applied in the field of mariculture disease prevention and control, can solve the problems of differences in immunogens of strains and other problems, and achieve significant differences in pathogenicity and immunogens, outstanding immune protection effects, and good complementarity Effect

Active Publication Date: 2020-07-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Claims
  • Application Information

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Problems solved by technology

From the previous epidemiological survey results, it has been confirmed that PDD is distributed in the breeding seas of many places in my country all the year round and with high abundance, but there are differences in the immunogens of the strains.

Method used

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  • Multivalent inactivated vaccine against photobacterium damselae subsp. damselae and preparation method thereof
  • Multivalent inactivated vaccine against photobacterium damselae subsp. damselae and preparation method thereof
  • Multivalent inactivated vaccine against photobacterium damselae subsp. damselae and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] A kind of mermaid Photobacterium mermaid subspecies multivalent vaccine, its preparation method comprises the following steps:

[0034] (1-1) First, activate the strains of the two strains from the strain box stored at -80°C. Under the condition of 28°C, use TSB agar plate medium for single colony purification and culture for 3 times, and each culture time is 20 -22h is better, not less than 20h, not more than 24h.

[0035] (1-2) Select a purified monoclonal colony from the TSB agar plate medium, inoculate it into 5ml TSB liquid medium, and culture it at 28°C and 180rmp / min shaking for 10-12h as the seed solution.

[0036] (1-3) Transfer 300ul of the above-cultured seed solution to 500ml of TSB liquid medium, and cultivate for 16-20h at 28°C and 180 rpm / min shaking. Draw 5ml and carry out serial dilution to determine the absorbance value (OD 600 ), according to Y=(0.6518X-0.0158)×10 9 The cfu / ml formula calculates the concentration of viable bacteria in the fermentat...

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Abstract

The invention belongs to the technical field of prevention and control of mariculture diseases, and particularly relates to a multivalent inactivated vaccine against photobacterium damselae subsp. damselae and a preparation method thereof. The preparation method comprises the following steps: screening two photobacterium damselae subsp. damselae strains with difference in pathogenicity and immunogen, respectively carrying out whole-bacterium inactivation on the two strains, adding Chinese wolfberry polysaccharide, and conducting mixing to obtain the multivalent inactivated vaccine. The vaccinehas an outstanding immune protection effect on marine fishes, and can effectively prevent and control infection of photobacterium damselae subsp. damselae.

Description

Technical field: [0001] The invention belongs to the technical field of mariculture disease prevention and control, and in particular relates to a multivalent vaccine of Photobacterium mermaida mermaid subspecies and a preparation method thereof. Background technique: [0002] Photobacterium damselae subsp.damselae is a subspecies of Photobacterium damselae, a bacterium of the genus Photobacterium damselae in Vibriaceae, first reported by American scientists Love et al. in Science magazine in 1981, isolated from Skin lesion of damselfish Chromis punctipinnis with skin ulcer disease. Subsequently, cases of Photobacterium mermaid subspecies Mermaid infecting fish, crustaceans, molluscs, sea turtles and other marine animals have been reported around the world. In addition, the bacteria can also infect marine mammals such as cetaceans and dolphins. It is also highly pathogenic to humans. It can infect humans and cause severe fasciitis or sepsis and cause death. It is a rare spe...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/116A61P31/04C12N1/20C12R1/01
CPCA61K39/0208A61K2039/521A61K2039/552A61K2039/55583A61K2039/70A61P31/04C12N1/20
Inventor 张正于永翔王印庚王学波罗展施琳妮孙心如张浩
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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