Holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with one-step method, and preparation method and application of holothurian aquaculture water modifier

A technology for cultivating water bodies and microorganisms, applied in biological water/sewage treatment, chemical instruments and methods, applications, etc., can solve the problems of ineffective purification of heavy metal ion pollution, reduction in quantity, poor adaptability of bacterial flora reproduction, etc. Good controllability of ups and downs, avoiding acidification and smelly bottom, and preventing sea cucumber diseases

Active Publication Date: 2014-04-02
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the micro-ecological water quality regulators used in aquaculture are limited to one or two types of probiotics to purify the aquaculture water, which has disadvantages such as poor adaptability to survival and reproduction of the bacterial flora and reduction in the number, and the purification of heavy metal ion pollution in the aquaculture water. no effect

Method used

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  • Holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with one-step method, and preparation method and application of holothurian aquaculture water modifier
  • Holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with one-step method, and preparation method and application of holothurian aquaculture water modifier

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1. Preparation of probiotic stock solution

[0036] Bacillus subtilis YB-1CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1CGMCC No.5115, which grew well on LB agar slant medium, were added to 2116E medium, pH7. 0~7.5, shake culture at 37℃ for 12~16h, then mix with volume ratio of 1:1:1 to prepare probiotic stock solution, the concentration of bacteria is about 1×10 8cfu / ml.

[0037] 2. Liquid fermentation culture

[0038] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0039] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0040] 3. Spray drying treatment

[0041] The adsorption carrier is prepa...

Embodiment 2

[0044] 1. Preparation of probiotic stock solution

[0045] Bacillus subtilis YB-1CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1CGMCC No.5115, which grew well on LB agar slant medium, were added to 2116E medium, pH7. 0~7.5, shake culture at 37°C for 12~16h, and then mix at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0046] 2. Liquid fermentation culture

[0047] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0048] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0049] 3. Spray drying treatment

[0050] The adsorption carrier is prepa...

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Abstract

The invention discloses a holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with a one-step method, and a preparation method and application of the holothurian aquaculture water modifier. Aiming at physiologic nature and cultivation characteristics of holothurian, and through use of the marine microbial technology, the holothurian aquaculture water modifier comprises bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117, which are separated and screened from the holothurian culture environment. The preparation method comprises the following step: using corn flour and soya bean flour as main culture materials, fermenting the materials at 28-37 DEG C for 24-48 hours, using a medical stone and rice husks as adsorption and suspension carriers, and processing to obtain the holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with the one-step method. The holothurian aquaculture water modifier is beneficial to the three-dimensional purifying effect of the marine aquatic water, can adsorb hazardous substances of heavy metal ions such as Hg2+, Pb2+, Cr6+, Cd2+ and Cu2+ in the water, greatly reduces the content of hazardous substances such as ammonia nitrogen, nitrite and hydrogen sulfide, deodorizes by using flowing water, effectively inhibits pathogenic microorganisms, prevents holothurian diseases, and improves yield and quality of holothurian aquaculture.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microbes, and specifically relates to a sea cucumber culture water improver for removing impurities and inhibiting the reproduction of harmful microorganisms in one step, as well as its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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