The invention relates to a preparation method of a pullulan polysaccharide based rice fresh keeping agent, and belongs to the technical field of fresh keeping of food grains. Pullulan polysaccharide is used as a film forming substrate, oxidation resistance of ascorbic acid is combined, and after two components namely the pullulan polysaccharide and the ascorbic acid are compounded, the pullulan polysaccharide based rice fresh keeping agent is prepared. The rice fresh keeping agent provided by the invention can be uniformly sprayed onto the surfaces of rice grains in the polishing operation procedure of rice processing, is convenient to use, and is easy to popularize and use. The rice fresh keeping agent provided by the invention has the advantages of being natural and safe in components, green and environmental-friendly in preparation technology, controllable in use cost, significant in fresh keeping effects and the like, the problems of ageing, mildew, rot and the like of the rice during storage period can be effectively controlled, the shelf life of the rice is prolonged, the freshness of the rice is reserved, and the rice fresh keeping agent has important practical significanceon solving the problem that the food grains are difficult to store.