66 results about "Bacillus flexus" patented technology

A microecological water quality conditioning agent applicable to restoring of sea cucumber culture water body and a method for preparing the microecological water quality conditioning agent belong to the technical field of aquaculture and microorganisms. By aiming at physiological habits and culturing characteristics of sea cucumbers and applying modern biological technique, the microecological water quality conditioning agent is made of bacillus flexus CGMCC (China general microbiological culture collection center) No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117, which are separated and sieved from a sea cucumber culturing environment and prepared by steps: using corn starch and soybean cake powder as main culturing materials, fermentating for 24-48h at 25-40 DEG C, and using zeolite and wheat bran as adsorption carriers for processing to obtain the microecological water quality conditioning agent. The microecological water quality conditioning agent is less susceptible to environment conditions, has remarkable purifying effect on sea cucumber culturing water body, and is capable of greatly reducing content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, deodorizing and effectively restraining pathogenic bacteria so as to prevent sea cucumber diseases and improve yield and quality of cultured sea cucumbers.

The invention discloses a compound microorganism preparation. The compound microorganism preparation comprises thermophilic fat bacillus, pseudomonas aeruginosa, streptococcus thermophilus, bacillus megatherium and bacillus flexus. The invention further discloses a preparing method of the compound microorganism preparation and application of the compound microorganism preparation to processing animal carcasses. Compared with the prior art, the compound microorganism preparation has the beneficial effects that macromolecular compounds, such as fat and celluloses in the animal carcasses and straw auxiliary materials, which are difficult to decompose can be efficiently and rapidly decomposed through the compound microorganism preparation, and the animal carcasses are converted into organic fertilizers with the low fat content; and the compound microorganism preparation further has the efficient deodorization function, the release amount of NH3 and H2S in the processing procedure can be effectively reduced, the stink concentration is reduced, and odorless processing is achieved. Finally, the animal carcasses are efficiently and safely processed into the organic fertilizers in an environmental protection manner, and resource utilization of the animal carcasses is achieved.

The invention provides a preparation method for composite bacillus microcapsule preparations. The method comprises the steps that bacillus subtilis YB-1 CGMCC No.5116 bacterial suspension, bacillus cereus YB-2 CGMCC No.5117 bacterial suspension and bacillus flexus XH-1 CGMCC No.5115 bacterial suspension are mixed and fermented to obtain fermentation products; protective agents and bacteria carrier fluid are added into the fermentation products, the mixture is fully shaken and sprayed in CaCl2 curing liquid to conduct curing, after curing is conducted, filtering, low temperature freeze drying, adsorption carrier absorbing and drying are conducted in sequence, and the composite bacillus microcapsule preparations are obtained. The composite bacillus microcapsule preparations have the advantages that the microbial content is high, intimidation of high temperature, oxygen, acid environment on composite bacilli can be effectively avoided, and the application effect of the composite bacilli in the aquatic animal breeding process is greatly improved.

A novel β-amylase having excellent practical applicability is found, and an object is to provide a practical use of the β-amylase. Provided is a method for improving a food, which is characterized in that a β-amylase derived from Bacillus flexus is acted on a food containing a polysaccharide or an oligosaccharide having an α-1,4 bond of glucose as the main chain.

The invention discloses a holothurian culture water quality restoration agent for effectively removing water eutrophication and a preparation method and application of the holothurian culture water quality restoration agent, belonging to the technical field of aquaculture and marine microorganisms. Aiming at the physiological habit and culture characteristic of holothurian, a marine microorganism technology is applied, the restoration agent consists of bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment, corn meal and soya bean meal serving as major culture raw materials are fermented at the temperature of 28-37 DEG C for 24-48 hours, and attapulgite, medical stone and rice hull serving as adsorption and suspension composite carriers are used for processing to obtain the holothurian culture water quality restoration agent for effectively removing water eutrophication. The product can adsorb harmful substances such as heavy ions including Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, and Cu<2+> in water bodies, and the content of harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide can be greatly reduced. Bottom odor and acidification of water bodies are avoided, pathogenic microorganisms are inhibited effectively, holothurian diseases are prevented, and the holothurian culture yield and quality are improved.

The invention discloses an endophytic bacillus flexus having salt resistance PE4941, which is preserved in Guangdong microbe bacterial preservation administration center with a preservation number being GDMCC 60176 on May 10th, 2017; and provides an application of the endophytic bacillus flexus for promoting vegetation growth under salt stress. According to the invention, the separated and screened limonium sinense endophytic bacteria bacillus flexus PE4941 has a plurality of growth-promotion characteristics such as nitrogen fixation and iron carrier generation, the endophytic bacteria can tolerate 8% of NaCl, limonium sinense seedlings can be inoculated by pouring root, a salt damage symptom under salt stress is obviously alleviated, biomass is increased, growth effect is promoted, salt resistance of the plants is increased, and the usage of a chemical fertilizer is reduced.

The invention discloses a compound microbial fertilizer for preventing and treating take-all disease of wheat and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer consists of the following raw materials: streptomyces catenulae, streptomyces albolongus, bacillus flexus, bacillus aryabhattai and trichoderma brevicompactum. The strains of the invention, on the basis of the comprehensive utilization of microbial inter-species or intra-species antibiosis, competition, hyperparasitism and bacteriolysis effects or by inducing the wheat to generate disease resistance by virtue of secondary metabolites, can generate various antibiotics, such as proceomycin, neomycin E, catenulin, diparomomycin and the like, so as to inhibit pathogenic bacteria of the take-all disease of wheat; the five strains adopted by the invention are directly separated from soil and have a rhizosphere growth promoting effect; the strains can promote reproduction and growth on crop surfaces or in plants or soil, and meanwhile, the strains can take an effect on nitrogen fixation and can promote the directional secretion of some secondary metabolites from plant rhizosphere; and the strains can enhance nutrition absorption of plants and stimulate plant growth, so that the effects of the fertilizer are achieved.

The invention discloses a composite microbial inoculum with growth-promoting and yield-increasing effects on capsicum and an application thereof. The composite microbial inoculum comprises fermentation liquor of Bacillus flexus HGD12, Pseudomonas putida HGD3 and Bacillus velezensis HP9 strains, wherein the Bacillus flexus HGD12 is Bacillus flexus and is preserved in China Center for Type Culture Collection on April 1, 2019, and the preservation number of the Bacillus flexus HGD12 is CCTCC NO: M 2019224; the Pseudomonas putida HGD3 is Pseudomonas putida and is preserved in the China Center forType Culture Collection on April 1, 2019, and the preservation number of the Pseudomonas putida HGD3 is CCTCC NO: M 2019223; and the Bacillus velezensis HP9 is preserved in the China Center for Type Culture Collection on April 8, 2019, and the preservation number of the Bacillus velezensis is CCTCC NO: 2019243. The composite microbial inoculum with the growth-promoting and yield-increasing effectson the capsicum and the application thereof have the advantages of promoting growth of the capsicum, improving yield of the capsicum, reducing application of chemical fertilizers and achieving reduction of the chemical fertilizer and increase of the yield of the capsicum.

The invention discloses a holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with a one-step method, and a preparation method and application of the holothurian aquaculture water modifier. Aiming at physiologic nature and cultivation characteristics of holothurian, and through use of the marine microbial technology, the holothurian aquaculture water modifier comprises bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117, which are separated and screened from the holothurian culture environment. The preparation method comprises the following step: using corn flour and soya bean flour as main culture materials, fermenting the materials at 28-37 DEG C for 24-48 hours, using a medical stone and rice husks as adsorption and suspension carriers, and processing to obtain the holothurian aquaculture water modifier for removing impurities and inhibiting harmful microbe propagation with the one-step method. The holothurian aquaculture water modifier is beneficial to the three-dimensional purifying effect of the marine aquatic water, can adsorb hazardous substances of heavy metal ions such as Hg2+, Pb2+, Cr6+, Cd2+ and Cu2+ in the water, greatly reduces the content of hazardous substances such as ammonia nitrogen, nitrite and hydrogen sulfide, deodorizes by using flowing water, effectively inhibits pathogenic microorganisms, prevents holothurian diseases, and improves yield and quality of holothurian aquaculture.

The invention discloses a compound nitrogen-fixing microorganism bacterium fertilizer and a preparation method thereof, particularly a compound nitrogen-fixing microorganism bacterium fertilizer which is wide in PH application range, strong in nitrogen fixing capacity under aerobic and anaerobic conditions, and easy to store. The compound nitrogen-fixing microorganism bacterium fertilizer is prepared from the following raw materials: 10 to 30 parts of bacillus flexus fermenting liquid, 30 to 80 parts of clostridium pasteurianum fermenting liquid and 20 to 60 parts of India Beijerinckia fermenting liquid. According to the compound nitrogen-fixing microorganism bacterium fertilizer and the preparation method thereof, the aerobic and anaerobic nitrogen fixing bacteria series are fused into a whole creatively; one type is the aerobic nitrogen fixing bacteria, namely, the India Beijerinckia (the India Beijerinckia can fix nitrogen under low oxygen, but is mainly the aerobic bacterium) and the bacillus flexus; the other type is the anaerobic nitrogen fixing bacteria, namely, the clostridium barati; both the aerobic nitrogen fixing bacteria and the anaerobic nitrogen fixing bacteria have strong nitrogen fixing capacity, can fix the nitrogen in relatively deep soil (10 to 30 cm), and can convert nitrogen in air into the nitrogen which can be absorbed by a plant and provide the nitrogen to the plant continuously.

The present invention belongs to the technical field of soil contamination biological treatment, and specifically discloses Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, wherein the Bacillus flexus HL-37 is preserved in Guangdong culture collection center on June 13, 2016, and has the preservation number of GDMCCNo.60045. According to the present invention, the aboriginal microorganism having the efficient cadmium activation function is screened from the farmland soil, and the Bacillus flexus HL-37 can activate the soil mineral nutrients at the rhizosphere, promote the plant growth, and effectively dissolve the carbonate binding state cadmium, the phosphate binding state cadmium and the cadmium having other main binding states in the soil, such that the soil heavy metal cadmium restoration efficiency of the super-accumulation plant is effectively improved; and during the soil heavy metal cadmium activation process with the strain, any discharged environmental pollutants are not produced, and the Bacillus flexus HL-37 has great application prospects in preparation of environment-friendly soil improvers.

The invention discloses a sea cucumber cultivation water quality repair agent capable of effectively reducing the water turbidity, a preparation method and use thereof, belonging to the technical field of aquatic product cultivation and marine microorganism. The repair agent disclosed by the invention aims at the physiology behaviors and cultivation characteristics of the sea cucumbers and uses the marine microorganism techniques. Bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117 are separated and screened from the sea cucumber cultivation environment, horn flour and soya bean meal are used as the main cultivation raw materials, and the bacillus flexus CGMCCNo.5115, bacillus subtilis CGMCCNo.5116 and bacillus cereus CGMCCNo.5117 are cultivated in the culture medium at 28-37 DEG C for 24-48 h, and then diatomaceous earth, sepiolite and rice hulls are used as absorption and suspension composite supporters to dry the fermentation products, so as to provide the sea cucumber cultivation water quality repair agent capable of effectively reducing the water turbidity. The repair agent has high tolerance for water environment and good floatability, suspension and ups and downs controllability, and can absorb harmful substances such as heavy metal ions such as Pb<2+>, Cr<6+>, Cd<2+>, Cu<2+> in the water, greatly reduce the content of the harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, avoid water acidification and bottom stinking-up, effectively inhibit pathogenic microorganism, and prevent the occurrence of diseases of the sea cucumber.

The invention relates to a holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians, and a preparation method and application thereof. The preparation method comprises the following steps: through a marine microorganism technology, based on physiological habits and culture characteristics of holothurians, separating and screening Bacillus flexus CGMCC No.5115, Bacillus subtilis CGMCC No.5116 and Bacillus cereus CGMCC No.5117 from a holothurian culture environment; selecting corn flour and bean pulp flour as main culture raw materials, and fermenting at 28-37 DEG C for 24-48h; and selecting zeolite, meerschaum and rice hull as adsorption and suspension compound carriers, and processing to prepare the holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians. The product has high tolerance to the water body environment and has favorable floatability, suspensibility and floating / sinking controllability, thereby being beneficial to three-dimensional stereoscopical purifying effect of a marine culture water body; and harmful substances such as Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, Cu<2+>, other heavy metal ions and the like in the water body can be adsorbed, thus greatly reducing the content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, preventing occurrence of holothurian disease and increasing the culture output and quality of holothurians.

The invention discloses a beta-amylase mutant with improved heat stability, and belongs to the technical fields of genetic engineering and enzyme engineering. The beta-amylase mutant with improved heat stability is finally obtained through researching the orthogenesis of beta-amylase derived from Bacillus flexus and carrying out heat stability screening on parent beta-amylase by an error-prone PCR technology which is one of most frequently used methods. The half-life of the mutant Thr461Tyr / Asp506Asn / Asp519Lys at 55 DEG C is two times higher than that of wild beta-amylase, and the mutant always keeps high enzyme activity at 55-65 DEG C. The mutant is more suitable for being in the industrial application of starch sugar than the wild beta-amylase.

The invention discloses a bactericide and process for producing an agricultural amino acid fertilizer bydetoxifying and hydrolyzing a rape seed cake. Bacillus flexus NJNpd41 is collected in China General Microbiological Culture Collection Center on 31, December 2012, with a collection number of CGMCC NO.7072. A method for producing the agricultural amino acid fertilizer by hydrolyzing the rape seed cake by using the NJNpd41 comprises the steps: regulating the content of the rape seed cake to 50 percent, and inoculating an NJNpd41 bacterium solution into the rape seed cake, and performing solid fermentation to obtain the agricultural amino acid fertilizer. By using the agricultural amino acid fertilizer produced by adopting the invention, the content of toxins contained in the rape seed cake can be reduced, the growth of an eggplant plant is remarkably promoted, the yield of eggplant is increased, and the quality of the eggplant is improved.

The invention relates to a tobacco plant growth promoting bacterium YC4 and application thereof. The tobacco plant growth promoting bacterium is a tobacco plant growth promoting bacterium YC4 which is classified and named as bacillus flexus, and the bacillus flexus is preserved in China General Microbiological Culture Collection Center on October 31, 2014, with the preservation number of CGMCC No. 9892. The tobacco plant growth promoting bacterium disclosed by the invention can be used for effectively converting poorly soluble potassium-containing silicate into soluble potassium salt and decomposing organic phosphorus, so as to improve the utilization rate of a fertilizer; the tobacco plant growth promoting bacterium is effectively applied to the cultivation of tobaccos and is capable of promoting the growth and increasing the output of the tobaccos, thereby being a great innovation in microorganism and tobacco planting.

The present invention discloses one kind of new Bacillus flexus LF-3 in the preservation number of CCTCC M 206128. The Bacillus flexus LF-3 can degrades phytotoxin effectively, and eliminate toxicity of the alkaloid 2, 2, 6, 6-tetramethyl piperidone (TMPD) extracted and separated from the above-ground parts of Oxytropis glacialis as one kind of poisonous grassland weed. The present invention is significant in eliminating the poisoning of Oxytropis glacialis and the biological control and ecological research of Oxytropis glacialis.

The invention discloses a water restoration agent for holothurian culture by employing attapulgite powder and a rice husk as carriers, and a preparation method and application of the water restoration agent. The water restoration agent is prepared from bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment by applying a marine microorganisms technology according to the physical habits and the breeding characteristics of holothurians. The method comprises the following steps: fermenting for 24-48 hours at 28-37 DEG C by taking corn powder and soybean meal as main composts, and then processing by taking the attapulgite powder and the rice husk as adsorption and suspension carriers, so as to prepare the water restoration agent for holothurian culture by employing the attapulgite powder and the rice husk as the carriers. The water restoration agent is strong in tolerance on a water environment, and good in floatability, suspension property and up and down controllability, a three-dimensional purification effect of seawater aquaculture water is facilitated, hazardous substances of heavy metal ions such as Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+> and Cu<2+> in water can be adsorbed, pathogenic microorganisms are effectively inhibited, a holothurian disease is prevented, and the yield and the quality of holothurian culture are improved.

The invention provides a microbial inoculant used for treatment of municipal sewage. The microbial inoculant is composed of bacterial powder A, bacterial powder B, bacterial powder C and auxiliary materials, wherein the bacterial powder A contains lactic acid bacteria, bacillus subtilis, blue-green algae, saccharomycetes and trichoderma; the bacterial powder B contains Nocard's bacillus, streptomycetes, thermophilic actinomycetes, aspergillus niger and chaetomium globosum; the bacterial powder C contains aspergillus, bacillus cereus, rhizobium, bacillus flexus and pseudomonas; the auxiliary material A is composed of dipotassium phosphate, potassium dihydrogen phosphate and sodium chloride; the auxiliary material B is composed of polyaluminium chloride, sodium lignin sulfonate and sodium laurate. The inoculant is used for treatment of municipal sewage, the microbes are reasonably mixed, the municipal sewage can be treated to form water conforming to the primary emission standard within three days, and no environmental pollution is caused during the treatment process.

A method of making a flavoured sweetener or food product by incubating an unrefined plant extract containing sucrose as the main solute with a microorganism or microorganisms to form a modified unrefined plant extract; evaporating water from the modified sucrose-based plant extract to form a concentrate; and cooking the concentrate to develop colour and flavour to produce the flavoured sweetener is disclosed. The flavoured sweetener can serve as a coconut sugar substitute. In a preferred embodiment the unrefined plant extract comprises sugarcane juice or sugar beet juice, and the microorganisms may be selected from Stenotrophomonas maltophilia, Bacillus subtilis, Bacillus flexus, or a Klyveromyces species. The flavoured sweetener can be used to make a range of food and beverage ingredientsand also food products including sauces, natural flavour extracts and flavour molecules, chocolate, health foods and convenience forms of the various forms of flavoured sweeteners.

The invention relates to relates to a peanut root growth-promoting bacterium HS9 and application thereof. The peanut root growth-promoting bacterium HS9 is classified and named as bacillus flexus, and collected in China General Microbiological Culture Collection Center on October 31, 2014, with the collection number of CGMCC No. 9889; the peanut root growth-promoting bacterium HS9 is capable of effectively converting slightly soluble potassium-containing silicates into soluble potassium salts, decomposing organic phosphorus and increasing the utilization rate of fertilizers; the peanut root growth-promoting bacterium HS9 is effectively applied to peanut planting, and is capable of promoting the growth of the peanut and increasing the peanut yield, and is a great innovation in microorganisms and peanut planting.

The invention relates to a bacillus flexus strain. The preservation name of the strain is bacillus flexus R29-2, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.18731. The strain is derived from the west Pacific Caroline seamount, and is a circular beige smooth opaque bacterial colony in an LB agar culture medium. The strainis in a short rod shape under a microscope, and gram staining is positive. The strain has a good antibacterial effect on gram-positive bacteria such as staphylococcus aureus, streptococcus agalactiae, enterococcus faecalis and bacillus cereus, and can effectively increase the survival rate of poultry, reduce the feed conversion ratio and improve the disease resistance of aquaculture animals whenbeing prepared into a micro-ecological preparation and serving as a feed additive in poultry breeding and aquaculture.

The invention discloses a lake riverway ecological restoration agent. The lake riverway ecological restoration agent is prepared from, by weight, 60-80 parts of nitrifying bacteria, 20-30 parts of mycobacterium szulgai, 20-30 parts of bifidobacteria, 16-20 parts of yeast, 6-8 parts of neutral citrobacter, 6-8 parts of bacillus subtilis, 6-8 parts of Yulei bacteria, 6-8 parts of actinomycetes, 6-8parts of thiobacillus, 6-8 parts of thermophilic desmolysing bacteria, 6-8 parts of bacillus cereus, 6-8 parts of bacillus flexus, 6-8 parts of paenibacillus, 6-8 parts of brevibacillus choshinensis 6-8 parts of saccharomyces cerevisiae, 6-8 parts of corn flour, 6-8 parts of soybean meal, 16-20 parts of oxidant, 10-12 parts of flocculant and 40-60 parts of carriers. According to the lake riverwayecological restoration agent, through the aerobic and anaerobic respiration of the nitrifying bacteria, mycobacterium szulgai, bifidobacteria, yeast, neutral citrobacter, bacillus subtilis, actinomycetes, thiobacillus, thermophilic desmolysing bacteria, bacillus cereus, bacillus flexus, paenibacillus, brevibacillus choshinensis and saccharomyces cerevisiae, organic and inorganic substances in water can be decomposed and absorbed, and the eutrophication root of the water is cut off.

The invention discloses a composite carrier micro-ecologic water purification agent for holothurian culture, and a preparation method and application of the agent. Based on the physiologic properties and culture characteristics of holothurian, by virtue of a marine microorganism technique, the agent is composed of bacillus flexus CGMCCNO.5115, bacillus subtilis CGMCCNO.5116 and bacillus cereus CGMCCNo.5117 separated and screened from a holothurian culture environment. The preparation method comprises the following steps: with corn flour and soybean meal as main culture raw materials, fermenting for 24-48 hours at 28-37 DEG C; and finally processing in the presence of medical stone, sepiolite and rice husk serving as an adsorption and suspension composite carrier, thus obtaining the composite carrier micro-ecologic water purification agent. The agent has good water environment tolerance, good floatability, suspension property and sinking and floating controllability and is beneficial to purification effect on seawater culture water in a three-dimensional manner, the water is prevented from acidification, smelly water bottom is avoided, pathogenic microorganisms are effectively inhibited, and the yield and the quality of holothurian culture are improved.

The invention provides a microbial agent for preventing and treating crop root system diseases and an application thereof, and belongs to the technical field of microbial preparations. The microbial agent comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus flexus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of an antioxidant and 4-9 parts of a stabilizer. The microbial agent can prevent and treat the crop root system diseases, and can prevent and treat pepper bacterial wilt, strawberry root rot, soybean root rot and cucumber fusarium wilt. After the microbial agent provided by the invention is used, the crop root system diseases can be prevented and treated, and the prevention and treatment effects are 85% or above.

To provide novel β-amylase derived from a microorganism and a gene thereof. β-amylase derived from Bacillus flexus is provided.