Unlock instant, AI-driven research and patent intelligence for your innovation.
Lyophilized powder of Photobacter lumina and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of bright photobacteria and freeze-dried powder, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as differences in cryoprotective effects, and achieve good stability
Active Publication Date: 2017-12-29
EAST CHINA UNIV OF SCI & TECH +1
View PDF4 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, because the concentration of each protective agent, the ability of cell penetration, etc. are not the same, the effect of cryoprotection will be different.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
[0058] The bacteria are collected after cultivation, that is, the bacteria are centrifuged to remove the supernatant, and the preparation method is as follows:
[0059] Take a Photobacterium phosphoreum EP tube and inoculate the bacterial solution into a 250ml Erlenmeyer flask containing 50ml of culture medium. According to the previously optimized culture conditions, the inoculation amount is 2%, 200r / min, pH 7.0, Shake culture at 18°C for 16-20h.
[0060] The formula of described culture medium:
[0061]
[0062]
[0063] Place the cultured bacterial solution in a centrifuge tube, centrifuge at 7000r / min for 30min, remove the supernatant, wash with 1ml of 3% NaCl solution, centrifuge, remove the supernatant, and collect the precipitate, which is the obtained said bacteria;
[0096] The thalline is the material collected after cultivation, that is, the thalline after the bacterial liquid is centrifuged to remove the supernatant, and the preparation method is the same as in Example 1.
[0097] Place the cultured bacterial solution in a centrifuge tube, centrifuge at 9000r / min for 20min, remove the supernatant, wash with 1ml of 3% NaCl solution, centrifuge, remove the supernatant, and collect the precipitate, which is the obtained said bacteria;
[0098] The preparation method of the lyophilized powder of Photobacterium lumina is as follows:
[0099] (1) The bacteria are added to the mixture of skim milk, trehalose, arginine and sodiumchloride, dispersed, then placed in an ampoulebottle, pre-cooled at -20°C for 1 hour, and then pre-frozen at -70°C 3 hours;
[0100] (2) Put the pre-frozen ampoules int...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses photobacterium phosphoreum freeze-dried powder and a preparation method thereof. The photobacterium phosphoreum freeze-dried powder comprises the following components in percentage by weight: 25-35 percent of skimmed milk, 20-24 percent of mycose, 0.7-1.0 percent of arginine, 1-1.5 percent of sodiumchloride and the balance of thalli. The photobacterium phosphoreum freeze-dried powder adopts a compound cryoprotectant; and experiments prove that the compound cryoprotectant is greatly improved in freezing resistance, has a higher protective property than a simplex protective agent and has good stability. The average value of luminous intensity of the photobacterium phosphoreum freeze-dried powder provided by the invention is about 1,843mv and approximate to a model expected value 1,820. When the toxicity of ZnSO4.7H2O, K2Cr2O7 and C6H5OH is detected with photobacterium phosphoreum freeze-dried powder prepared in different batches, the results show that the EC50 value variation coefficients detected by the photobacterium phosphoreum freeze-dried powder prepared in different batches are less than 6%.
Description
technical field [0001] The invention relates to a preparation of luminescent bacteria and a preparation method thereof. Background technique [0002] Based on the current situation of environmental pollution in my country, the detection method of luminescent bacteriatoxicity is an efficient and convenient means to determine the biological toxicity of pollutants. The biggest advantage of the luminescent bacteria toxicity detection method is that it can be detected on-site, the method is fast, accurate, and easy to operate. There is a significant negative correlation between the relative luminosity of luminescent bacteria and the comprehensive toxicity of water. Using photoelectric detection technology to use luminescent bacteria that are sensitive to the environment for the detection of water toxicity has the advantages of simplicity and speed. On-site toxicity testing requires fast and simple operation, but fresh bacterial solution is inconvenient to carry and difficult to...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More