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Preparation and application methods of photobacterium damsela vaccines of cynoglossus semilaevis

A technology of photobacteria and half-smooth tongue sole, applied in the field of animal disease-resistant vaccine preparation, to achieve good immune response, improve survival rate, and enhance immunity

Inactive Publication Date: 2012-10-24
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no research and application of anti-disease vaccine for semi-smooth tongue sole. At present, most farmers use chemically synthesized drugs or antibiotics to control the occurrence and prevalence of diseases. There is no anti-photobacteria mermaid vaccine suitable for semi-smooth tongue sole in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. A preparation method of Photobacterium mermaida vaccine of semi-smooth tongue sole, which uses Photobacterium mermaidus ST1 ( Photobacterium damselae ST1) as the immunogen, with astragalus extract as the adjuvant; the specific steps are as follows:

[0022] (1) Photobacterium mermaid ST1 ( Photobacterium damselae ST1) The strain was inoculated on ordinary nutrient agar broth medium, cultured at 28°C for 24 hours, added formaldehyde with a final concentration of 0.3% at 28°C for 48 hours to obtain inactivated bacteria;

[0023] (2) Soak Astragalus membranaceus in distilled water for 30-60 minutes, decoct, filter, concentrate until the final concentration is 1g / mL of the original drug, and obtain Astragalus membranaceus extract;

[0024] (3) The inactivated bacteria were mixed with the obtained astragalus extract to prepare the water-based Astragalus adjuvant Photobacterium mermaida vaccine; the immunogen of Photobacteria mermaidus in the vaccine was 1.17×1...

Embodiment 2

[0025] Example 2. A method for using the photobacterium mermaid vaccine of semi-smooth tongue sole prepared by the preparation method described in Example 1. It adopts the method of immersion immunization. Firstly, the semi-smooth tongue sole is soaked in an 8% NaCl solution under aerated conditions for 5 minutes, and then the semi-smooth tongue sole is diluted with water at a volume ratio of 1:5 to obtain a half-smooth tongue sole Photobacterium mermaid vaccine solution, and then the The half-smooth tongue sole was soaked in the photobacterium mermaid vaccine solution of half-smooth tongue sole with aeration for 1 hour, and one week later, the immunization was boosted once by the above-mentioned method.

Embodiment 3

[0026] Example 3. A method for using the photobacterium mermaid vaccine of semi-smooth tongue sole prepared by the preparation method described in Example 1. It adopts the method of injection immunization. The semi-smooth tongue sole with a body length of 15-20cm is used for initial injection immunization with an immunization dose of 0.2mL / tail, and then boosted immunization once every other week with the above-mentioned immunization dose, for a total of 4 immunizations.

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PUM

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Abstract

The invention provides a preparation method of photobacterium damsela vaccines of cynoglossus semilaevis, which is characterized by comprising the steps of: inoculating ST1 strains of photobacterium damsela on an ordinary nutrient agar bouillon culture medium, cultivating for 24h at the temperature of 28 DEG C, and adding formaldehyde with the final concentration of 0.3% into the culture medium to inactivate the photobacterium damsela for 48h at the temperature of 28 DEG C so as to obtain inactivated bacteria; soaking milkvetch roots with distilled water, and then decocting, filtering and concentrating to obtain milkvetch lixivium; and mixing the inactivated bacteria and the obtained milkvetch lixivium to prepare the photobacterium damsela vaccines of the cynoglossus semilaevis. In the invention, the vaccines have the advantages of enabling the cynoglossus semilaevis to produce good immune response, enhancing the immunity of the cynoglossus semilaevis to the pathogen (photobacterium damsela), and increasing the survival rate of cultivated cynoglossus semilaevis, the output of the cultivated cynoglossus semilaevis, and the economic benefit of the cultivation and production of the cynoglossus semilaevis. The problem that the cynoglossus semilaevis is easy to catch diseases caused by the photobacterium damsela during the cultivation and production process at present is fundamentally solved. The invention also discloses an application method of the photobacterium damsela vaccines of the cynoglossus semilaevis.

Description

technical field [0001] The invention relates to a preparation method of an animal disease-resistant vaccine, in particular to a preparation method of a semi-smooth tongue sole mermaid photobacterium vaccine. The invention also relates to methods of using the vaccine. Background technique [0002] Photobacterium mermaidi widely exists in seawater and parasitizes marine animals. It mainly infects ectothermic animals, especially farmed fish. In addition to infecting damselfish fish, it also infects sharks, turbot, yellowtail, half smooth tongue sole, etc. The harm of production is quite serious. In recent years, with the expansion of the breeding scale of semi-smooth tongue sole, the diseases caused by Photobacterium mermaidi have caused great losses to the production of semi-smooth tongue sole. Therefore, the effective prevention of diseases caused by Photobacteria mermaidus, In order to minimize its harm, the economic benefits of tongue sole farming will be significantly imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/106A61K39/39A61P31/04
Inventor 张晓君阎斌伦
Owner HUAIHAI INST OF TECH
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