38 results about "Every other week" patented technology

The present invention discloses a planting method of the stem, leaf, bolt and head of field high-selenium garlic; the puke garlic of Cangshan is taken as the seed; people select field with more than 1.2 percent of organic material and implement disinsection treatment on soil seed before plantation; people strictly manage the field and apply selenium fertilizer made of aspartic acid, threonine, serine, glutamic acid, cysinic acid, glycin, arginine, total ammonia acid, nitrogen, phosphor, kalium, Na2SeO3, etc. on the leaf every other week. The selenium contents of the root, stem, leaf, bolt and head of the planted high-selenium garlic are separately between 21.75 and 35.83mg / kg, between 9.83and 20.12mg / kg, between 19.76 and 33.89mg / kg which are separately 311 times, 328 times and 725 times of that of the root, stem, leaf, bolt and head of garlic which are not added with selenium; the present invention is convenient for large-area field plantation and the root, stem, leaf, bolt and head of the planted high-selenium all contains active selenium which is beneficial to the health care of people; the raw material and extract can be used to make troche, capsule, injection, spray, oral liquid, health care food or commodity for hairdressing and skin protection integrating the functions of curing disease, health care and hairdressing.

The invention discloses a dry lotus root planting method. The dry lotus root planting method comprises the following steps: 1) selecting dry lotus root seed tubers without rot or lesions, drying the dry lotus root seed tubers in the sun for 2-4 hours, then carrying out sterilization and seed soaking treatment on the dry lotus root seed tubers and placing the dry lotus root seed tubers in a shade place for two days and then planting the dry lotus root seed tubers; 2) deeply ploughing a planting field by 40-50cm, then levelling the planting field and digging fertilizer furrows and planting furrows; 3) planting the dry lotus root seed tubers treated in the step 1) in the planting furrows and covering the dry lotus root seed tubers with soil, wherein the planting space between the dry lotus root seed tubers is 40cm; 4) watering the dry lotus root seed tubers once the next morning after planting, later watering the dry lotus root seed tubers once every other week, and after dry lotus root seed tuber sprouts grow 5cm above the ground, applying leaf fertilizers once and spraying insecticides once every other half a month later. The dry lotus root planting method has the beneficial effects of being green and environment-friendly; being high in the final dry lotus root yield; being high in starch content of the dry lotus roots and having no chemical pesticide residue, thus conforming to the green and environment-friendly ideas.

The invention belongs to the technical field of crop planting and discloses a high-yield planting method of a thin-shelled walnut. The method comprises the following steps of garden establishment: selecting well-structured and fertile sandy soil with strong water retaining capacity, performing deep ploughing and applying base fertilizer containing a soil conditioner, transplanting: performing transplanting and field planting from September to November, water and fertilizer management: performing top dressing before walnut germinating and blossom drop and in a fruit hardness period, applying different additional fertilizer according to walnut growth requirements in different periods, applying enough organic fertilizer before land freezing, shaping and pruning: performing no pruning at the year of the field planting and the transplanting, and disease and pest control: spraying 0.5-1B lime sulphur once after leaf falling and before the germinating, and spraying 0.5% phoxim powder or 50% thiophanate methyl 800-multiple liquid for prevention and control every other week from the middle of June. The planting method of the walnut improves a soil environment, ensures nutritional requirements of the walnuts in the different periods, promotes early blooming and fruitfulness of the walnut, increases a walnut yield, improves walnut quality, reduces fertilizer input and improves economic benefits.

The invention belongs to the technical field of plant cutting propagation and discloses an acer pentaphyllum cutting propagation method. The method includes steps: acquiring acer pentaphyllum branches, trimming into short branches in length of 5-8cm to serve as cuttings, trimming, soaking bases of the cuttings into water as soon as possible, and spraying clear water to wet leaves; preparing a mixed substrate from perlite and peat in a volume ratio of 6:4, disinfecting the mixed substrate, and adding into deepened trays with tray covers; soaking the bases of the cuttings in rooting liquid for 0.5h, inserting into the mixed substrate, and compacting immediately, wherein holes and the cuttings are in one-to-one correspondence; putting the deepened trays into a simple culture chamber, controlling the temperature at 22-25 DEG C, keeping humidity in the deepened tray to be 100% and illuminating intensity in a range of 700-1200lx, irrigating the cuttings and the nearby mixed substrate with 40ml of rooting liquid at a time interval of 3d, and totally irrigating for 3 times; 10d after cuttage, spraying a disinfectant once every other week until rooting of all cuttings is realized. Components of the rooting liquid are simple, and low cost, step simplicity, freeness of complexity and high cuttage rooting rate and survival rate are realized.

The invention relates to a polluted soil repairing method, in particular to a method for repairing acid heavy metal polluted soil by means of a complex improver. The method comprises the steps that 1, acid soil with lead and zinc is air-dried and crushed; 2, the crushed soil is added with calcium oxide with the particle diameter being 0.1-0.5 cm; 3, the soil is added with 2-10% of calcium magnesium phosphate; the processed soil is aged at indoor temperature for 30-45 days and overturned and stirred every other week, water is supplemented, and the water content is kept being 50-60% of the field moisture capacity; 4, the aged soil is added with 5-10% of sludge fermentation fertilizer, and then the improved soil is obtained. According to the method, by means of repair of the chemical improver and aging treatment of heavy metal such as lead and zinc in the soil, the heavy metal pollution is repaired, the soil property and fertility are improved, and it is ensured that the repaired soil can be used for normal agriculture and forestry production.

The invention discloses a prevention and treatment method of edible lily anthracnose; the method comprises the steps of preparing the medicament prepared by the following raw materials by weight: 1 part of 25% prochloraz missible oil, 10-15 parts of celastrus orbiculatus, 8-10 parts of radix euphorbiae lantu, 8-12 parts of Rhizoma Coptidis, 5-10 parts of Zanthoxylum armatum, 2-3 parts of Royal Paulowinia Flower extract, 10-15 parts of potassium chloride and 1-2 parts of boron fertilizer; mixing the raw materials, adding 1200-1500 times amount of water to the mixture and evenly spraying on the whole plant in the annual June-July before the plant are affected with edible lily anthracnose or when early scabs appear, and spraying for 1-3 times every other week; the method provided by the invention has significant prevention and treatment effect on the edible lily anthracnose and the prevention and treatment effect is good; the prevention and treatment method can effectively inhibit the occurrence and development of anthracnose, so as to increase the high yield of edible lily.

The invention discloses a planting method for improving qualities of mangoes, and belongs to the technical field of fruit cultivation. The planting method for improving the qualities of the mangoes comprises the following steps that (1) land is selected; (2) soil is prepared, the land is cleaned up and a special quality fertilizer is applied, deep sowing is carried out in one month before planting, a base fertilizer is applied, and the base fertilizer is mixed with topsoil to turn back to a cave; (3) seedlings are selected and planted, plants without pests and diseases are selected as new seedlings, fix planting is carried out in cloudy or rainy days, roots are watered adequately, and a soil layer is covered with straws; (4) fix planting and fertilization are carried out, once topdressingis carried out every two months after the new seedlings sprout, urea is applied after flowers wither, the fertilizer is applied once to twice every month before fruiting, a fruit swelling fertilizer is applied before fruiting, and corresponding fertilizers are applied at corresponding growth stages; (5) fruit trees are trimmed, main branches are selected and part of the branches nearby are removed, pinching is carried out, and a nutrient is sprayed once every other week; and (6) mangoes are picked up. The planting method for improving the qualities of the mangoes can promote the growth, earlyfruiting, and high and stable yield of mango trees.

The invention belongs to the field of floriculture methods, in particular to the field of colorful pepper cultivation methods. A method for enhancing ornamental quality of a colorful pepper comprises the steps of: when fruits begin to color, laying a silvery sun shading curtain under flowerpots and picking off 1-3 lightproof blades around the fruits or supplementing light for 4 hours by utilizingan LED (Light Emitting Diode) red light lamp at 18:00-22:00 everyday; and applying 0.2% of monopotassium phosphate every other week, wherein the cultivation temperature is 18-23 DEG C; the humidity is 75-80%; the illumination is 15000-18000Lx; and tide-type flood irrigation is adopted as a fertilizer water irrigation way. By utilizing the cultivation measure provided by the invention, the ornamental quality of the colorful pepper is greatly enhanced; the fruit quantity is increased; the size is uniform; the color is pure and bright; the blades are massive and glossy; and the plant type is compact.

The invention discloses a soil in-situ remediation and heavy metal removing method. The method comprises the steps that a, a composite material is diluted with water and then sprayed to heavy-metal contaminated soil according to the proportion of 100-200 L / mu, and the composite material is applied every other week and applied continuously for one month; b, modified Chinese medicine residue and organic manure are mixed and sown in the heavy-metal contaminated soil by adopting a tractor-ploughing method; and c, weighing method watering is adopted for keeping the soil water content to be 50-60% of the treated soil water-holding capacity and is performed for 7-10 days successively. Soil contaminated with various heavy metals such as Pb, Cd, Cu, Zn, Cr and As and toxic organic substances such as chlorinated organic compounds, bromo-organic matters and antibiotics can be remediated, the form that the heavy metal activity and biological availability in the soil are high is relieved, the heavymetals are prevented from migrating and transforming to plants from the soil, biological concentration and amplification of the heavy metals are avoided, the heavy metals such as Cr and Hg in the soil can be efficiently removed, the original functions of the soil are restored, agricultural development requirements are met, the ecological safety of the plants such as crops is guaranteed, and accordingly, ecological risks, caused by biological amplification and concentration of the heavy metals, to the people are avoided.

The invention discloses a cultivation method of a potted strawberry. The cultivation method comprises the following steps of 1 container selecting, wherein a barrel type clay pot with the good breathability is selected; 2 nutritional soil preparing, wherein 3 parts of mature compost, 4 parts of vegetable garden soil, 5 parts of river sand and 1 part of mature cake fertilizer are taken by mass and then fully mixed to be uniform; 3 variety selecting, wherein a strawberry which is spread in plant and long in flowering period and can fruit multiple times is suitable for being selected; 4 potting, wherein potting is conducted in autumn, and the strawberry is placed in a shade place for 3-5 days after being watered thoroughly and then moved to a place with enough light; 5 top dressing in time, wherein a urea solution with the concentration of 0.2% to 0.4% is sprayed three times before germination in spring, a monopotassium phosphate solution with the concentration of 0.2% is sprayed two times before flower bud differentiation, and after fruits are harvested, 20-40 g of compound fertilizer is sprinkled into the pot every other week; 6 seedling managing, wherein bud thinning, leaf picking and stolon removing are timely conducted; 7 repotting, wherein repotting is conducted once every two years. According to the cultivation method, the survival rate of the potted strawberry is greatly increased.

The invention relates to a recombinant Wzt protein rabbit serum polyclonal antibody and a preparation method thereof, which relate to the field of antibody preparation, and fill the gap of applying recombinant Wzt protein for preparing the specific polyclonal antibody. The preparation method of the polyclonal antibody comprises the steps of after mixing and emulsifying the recombinant Wzt protein and an equivoluminal freund's complete adjuvant, carrying out multiple sites subcutaneous injection on the back parts of female rabbits; carrying out secondary immunizing: after one week, after mixing and emulsifying the recombinant Wzt protein and the equivoluminal freund's incomplete adjuvant, carrying out multiple sites subcutaneous injection on the back parts of the female rabbits; carrying out thrice immunizing and quartic immunizing every other week according to a secondary immunizing process; collecting venous blood of female domestic rabbit ears after immunizing each time, and detecting a rabbit serum antibody titer; at one week after quartic immunizing, collecting rabbit whole blood, centrifugally collecting a supernatant to obtain a rabbit serum, and obtaining the recombinant Wzt protein rabbit serum polyclonal antibody after purifying. The recombinant Wzt protein rabbit serum polyclonal antibody has favorable specificity, and can be used for detecting Brucella Wzt protein.

The invention relates to a pear tree transplanting method, which is completed by the following steps: (1) pruning; (2) preparing a planting hole: determine the diameter of the planting hole according to 15 times the trunk diameter of the pear tree to be planted, and backfill the hole with straw , garbage and topsoil, filling to a position 20 cm above the ground; (3) tree lifting and handling: transplant pear trees with soil, and transport them to the transplanting site in time; (4) planting: according to the direction marks made during pruning The growth direction is put into the planting hole, and the filling must be 10 centimeters higher than the original topsoil; 0.5 grams of No. 3 ABT rooting powder is used to fill the root with 10 kilograms of water for the rooting water, so that the hole soil is drenched, and finally the planting hole is covered with plastic film; (5 ) Management after planting: use 0.2% potassium dihydrogen phosphate and 0.2% urea water to fertilize the leaves every other week when spraying water; carry out disease and insect control in time during the growing season, and use lime sulfur mixture to clear the garden and whiten the trunk during the dormant period. Can. The beneficial effects of the invention are: the supply of nutrients to pear trees can be guaranteed for a period of time, the survival rate after transplanting can be improved, and the planting needs can be met.

The invention discloses a preparation method of Qianliang tea. The preparation method comprises the following steps: picking one-bud three-leaf fresh leaves, and performing fixation and rolling; performing primary pile fermentation treatment, then performing rolling again and sorting, performing secondary pile fermentation treatment, and performing fermentation to obtain secondarily fermented leaves; steaming the secondarily fermented leaves, and putting the steamed leaves into a bamboo basket in batches to be extruded into tea columns until a plurality of tea columns form a Qianliang tea column, wherein the top surface of every layer of pressed tea column is sprayed with bacterial liquid containing eurotium cristatum thalli, and then, the next layer of tea column is pressed; placing a plurality of Qianliang tea columns at intervals, performing flower growth for 1-2 months, and stirring the Qianliang tea columns every other week; and gradually heating the Qianliang tea columns after flower growth to 30-35 DEG C, and drying the Qianliang tea columns until the moisture content is less than 10% to obtain the Qianliang tea. Golden flowers are uniformly distributed on the surface and the center of the Qianliang tea provided by the invention.

The present invention provides a pharmaceutical composition for treating cancer, the composition comprising as the active ingredient a compound represented by general formula I: C-[-CH2-O-(-CH2CH2-O-)­n-X1-CHR1-O-CO-NR2-CH2-X2]4 (I) (where X1-CHR1-O-CO-NR2 is a linker, X1 is a spacer, R1 is an optionally substituted C1-4 alkyl, R2 is a phenyl or substituted phenyl group, X2 is SN-38, and n is an integer of 200 to 1,000). The composition is characterized by the following: that the composition can be used in a subject having cancer at a dosage regimen that calls for parenteral administration of a single dose of approximately 0.01 to 11 µmol / kg body weight at a frequency of once a week every other week for at least two administrations; and that the composition does not have irinotecan-like adverse reactions but does impart the same or better cancer proliferation-inhibiting activity as irinotecan at a dose that is 1 / 10 (molar ratio) or less that of irinotecan. Also provided is a method for treating cancer.

The invention discloses a method for repairing cadmium-contaminated black soil by inter-cropping solanum nigrum L and soybeans. The method comprises the following steps that A, tillage is conducted ona land to be repaired, the depth of the tillage is 20-30 cm, calcium phosphate and potassium nitrate are evenly sprinkled in the tillage land, a mixture of the calcium phosphate and the potassium nitrate is sprinkled in three layers, and a wood pad layer is laid between layers of the mixture; B, fertilization is carried out on the tillage land; C, seedlings of the solanum nigrum L are planted onthe tillage land; D, 3-4 weeks after the solanum nigrum L is planted, topdressing is carried out; E, one week after the topdressing, the soybeans are planted, firstly, pits are dig on the surface of the soil, soybean seedlings are planted in the pits, and the outer sides of the soybean seedlings are laid with a circular fence after planting; F, watering is conducted on the land every other week, adisinfectant is added to the circular fence every two weeks for disinfection, and weeds in crops are removed. The method is capable of improving the deficiencies of the prior art and improving the efficiency of absorption of heavy metal contaminants in the soil.

The invention provides a cultivation method of hemerocallis fulva. The cultivation time is at the beginning of March in early spring, the cultivation is performed before germination, the hemerocallis fulva is cultivated and planted in soil with good drainage, deep soil layer, fertile and loose soil, no water accumulation in summer and abundant organic matters, when the hemerocallis fulva is planted, 2-3 kg of decomposed pig and cow dung fertilizer is applied in each square meter of the soil, when the hemerocallis fulva is transplanted, the plant-row spacing needs to kept at 30 cm*40 cm, the top dressing needs to be supplemented for 2-3 times before and during flowering phase, a phosphate-potassium fertilizer is mainly supplemented, 0.2% of potassium dihydrogen phosphate is sprayed for assistance, watering is performed once every other week, and the soil water-holding capacity is kept at 70%-80%. The cultivation method disclosed by the invention has the benefits that the germination rate of the hemerocallis fulva cultivated by the cultivation method disclosed by the invention is 98%, the survival rate is 92.8%, the hemerocallis fulva can normally enter into the repotting stage after 3-month cultivation, and an established large-scale commodity production system is stable and shorter in period, and can meet the large-scale cultivation production needs.

The invention relates to a biological removal method of siloxane in a biological energy source gas. The biological removal method comprises the following steps of: transplanting pseudomonas H4 into an LB (Luria Broth) culture medium from a preservation slope, and carrying out shaking culture at 30 DEG C to prepare a seed solution; inoculating the seed solution into an LB culture medium containing 0.1-0.5 percent of siloxane at an inoculum size of 10 percent, and carrying out shaking culture for 18-24 hours; mixing the LB culture medium with a culture medium for a bio-trickling filter according to a proportion of 10: 1, and soaking the bio-trickling filter for one day; then, circulating a soak solution at a flow speed of 1-10L / h to enable the pseudomonas to uniformly grow on ceramsite, and circularly culturing for one week to finish biofilm culturing; and introducing a siloxane-containing gas into the bio-trickling filter at a flow speed of 1-10m<3> / h; and culturing the culture solution, and replacing 1 / 3 of the culture solution every other week so as to supplement the fresh culture medium. The biological removal method can be used for improving the siloxane removal efficiency to above 50 percent and is small in equipment size and occupying area during industrial application and capable of basically realizing industrial application.

The invention discloses a citrus seedling grafting method. A fructus aurantii seedling sown on a seedbed last year is selected as a rootstock, a scion is treated with hot and humid air on the day of grafting, the hot and humid air contains tetracycline hydrochloride and 2,4-D, the scion which is cut is quickly inserted into a rootstock grafting union during the seedling grafting process, a grafting film is then used to seal the grafting union, a novel lipid membrane diluent is used to be sprayed on the coating part of the grafting film, a multi-functional tree grafting device is then used forfixing, a knife is used to cut a small opening on the grafting film in a covered surface of the sealed rubber, a plant growth regulator solution is then sprayed, the grafted citrus seedling is cultivated under suitable light and humidity conditions after the grafting, during the first 15 days after grafting, pesticide spraying on the grafting film in a covered surface of the sealed rubber is carried out for every 5 days and every 10 days, and the healing condition of the grafting union is checked for every other week. A disinfection function is achieved, formation of a scion separation layer is also prevented, infection of pathogens is isolated, and the survival rate of grafting is improved.

The invention discloses a method for controlling the scale insects of citrus trees. The method includes the following steps: (1) performing quarantine of seedlings, disinfecting seedlings with scale insects, commonly using methyl bromide for fumigation, causing the seedlings to have soil, using a pesticide amount of (40-60) ml for every square meter, and performing fumigation for 4 h; (2) in winter, cutting off too dense branches and worm branches, keeping a citrus orchard ventilated and transparent, creating an environment not suitable for survival of the scale insects, and spraying lime sulfur mixture to seal the orchard; (3) performing pesticide prevention: in the first egg hatching period of the scale insects in April to May, spraying 1000-fold liquid of 48% chlorpyrifos, 1800-fold liquid of 1.8% avermectin, 2000-fold liquid of 5% acetamiprid missible oil, 1000-fold liquid of 25% buprofezin wettable powder and 2000-fold liquid of 10% imidacloprid every other week; (4) performing biological control: as there are many kinds of scale insect natural enemies, strengthening the protection and performing artificial transfer and breeding release, concentrating the cut branches with thescale insects to an outer space outside the orchard, and burning the branches after a period of time. According to the method for controlling the scale insects of citrus trees, the scale insects canbe effectively controlled, and the vigor and yield of the citrus trees are recovered.

The invention discloses a method for breeding low-scented meat sheep, which comprises the following steps: S1, selecting 30-40 days old, tall and strong lambs to be raised in a sheepfold, placing rockery and fake trees in the sheepfold, grazing in daytime, supplementing the feed after entering the sheepfold, sterilizing once a day by medicine smoke, wherein the feed is made of the following raw materials by weights: silage corn stalk feed, honey, wheat bran, soy flour, algal polysaccharide, garlic essential oil, scallions flavonoids; S2, after the mutton sheep in step S1 is raised to 4 monthsold, the excrement of the sheep is increased, after the excrement is cleaned every 3 days, mixing the medicinal medicinal slag and the dry sawdust powder according to the ratio of 1:10-15 and uniformly spreading on the ground of the sheepfold, wherein the spread thickness is 15cm-18cm, changing the ground every other week, and re-spreading; S3, after the weight of mutton sheep is more than 40kg, sending the mutton sheep out of the sheepfold, and free grazing.

A control module for a generator exercise timer enables a user to conduct generator exercise sessions at intervals longer than predetermined intervals permitted by the manufacturer. The control module can be connected in series with a generator's existing electronic exerciser timer, preferably by disconnecting the existing exerciser timer's wiring harness from the exercise timer and connecting the control module to the exercise timer. The control module can be provided with a connector of its own to which the wiring harness can be connected. The control module includes a latching relay and a non-latching relay that can be operated in such a manner that alternating "engine start" signals are sent to the generator. Thus, the exercise timer will be effective to exercise the generator at delayed intervals, e.g., every other week rather than every week. The control module includes a pushbutton switch and a visible LED. The switch enables a user to control the state of the latching relay, while the LED indicates to the user whether the latching relay will permit or prevent the next engine start signal from being effective to start the generator.

The invention provides a simple collecting device for large fungal spores in forest air and application method for the simple collecting device. The device comprises a filter, a wild-mouth bottle anda support. During use, the filter is arranged on the wild-mouth bottle; the whole device is fixed on the support and is putted on collection points; the filter is cleaned by ultrapure water (Milli-Q)every other week; the wild-mouth bottle collected with filtrate is take down and is replaced with a new sterile wild-mouth bottle; the collected filtrate is further treated for obtaining the varietiesand the quantity of the large fungal spores in collection point air. The device for collecting the large fungal spores in the forest air has the advantages of simple operation and convenience in carrying. The workload of field collectors can be effectively reduced. Compared with field collection, the simple collecting device has the characteristic that a variety of large fungal spores can be quickly collected.

The invention discloses a cultivation method for sunlight seedlings in plastic cups, and particularly relates to the technical field of seedling culture. The cultivation method comprises the followingsteps: step one, selecting the plastic cups with the heights and diameters being 40* 30, and uniformly forming a plurality of ventilating holes in the bottom of each plastic cup; and step two, layinga stainless steel screen on the bottom of the cavity of each plastic cup, cutting dry straws, dead leaves and dry straw litters by using a forage cutting machine into chippings and then uniformly laying the chippings on the stainless steel screens of the plastic cups. A nutritional substrate is prepared, bean dregs, humus soil and a decayed organic fertilizer are added, dilute liquid manure is continuously sprayed in a growing process, plastic cup bodies rotate every other week, in cooperation of fine river sand, rice bran, the stainless steel screens and the chippings of the dry straws, thedead leaves and the dry straw litters, the soil in the plastic cups can always maintain in a good state, full nutrients are provided for growth of seedlings, the nutrition is comprehensive, the growthspeed of the sunlight seedlings is increased to be rapidest, the disease resistance is high, and the yield is also high.

The invention discloses a simple incubator, comprising a bubble cabinet with an upper cover. The upper part of the bubble cabinet is provided with a support grid plate. The moveable upper cover is provided with holes. A culture bottle passes through the hole of the upper cover and location holes of the support grid plate and extends to the lower part of the bubble cabinet. A bottle opening of the culture bottle is higher than the upper cover. A tampon is arranged in the bottle opening. The method comprises: in winter, placing the incubator on a place facing the sun in a room, detecting breeding condition of the drosophila melanogaster every other week, and changing fresh culture medium as needed; in summer, placing the incubator in a refrigerator preservation chamber at the temperature of 4 DEG C, taking the incubator out and placing in indoor environment for a day every other week, then detecting breeding condition of the drosophila melanogaster, and putting the incubator in a refrigerator to continue culture, at the time of 3-4 week, changing fresh culture medium as needed and subculturing. The bubble cabinet which is low in cost is used as the drosophila melanogaster incubator. The incubator is simple in structure and low in manufacturing cost. The incubator can ensure safe breeding of drosophila melanogaster population when being used in winter vacation or summer vacation, and the incubator satisfies requirements of drosophila melanogaster breed conservation breeding.

The invention provides a disease-resistant perch culture method. The method comprises the following steps of S1, selecting a quiet and safe environment to build a pond, wherein the area of the pond is8-12 mu, the water depth is 2-3 m, and the water surface every 1.2-1.5 mu is provided with an aerator; S2, drying the pond, dredging and conducting solarization on the pond, and disinfecting with 70-80 kg / mu of quicklime 14-16 days before seedling releasing; S3, desalinating fry; S4, daily feeding, wherein the fry is fed, the environment inside the culture pond is checked, water in the culture pond is changed every other week, high-density perch culture brings a lot of residual bait and excrement at the bottom of the pond, the decomposition concentration of harmful substances is high, the oxygen in the water is consumed in large quantities, which is more serious in hot weather, and counts as an important cause of fish diseases, components such as plant ash, chlorite, zeolite powder and the like are added to improve the decomposition efficiency of the residual bait and the excrement, the consumption of the oxygen in the water is reduced, the outbreak of the fish diseases is avoided, the water quality is purified, the oxygen content of the water is increased, and the yield per unit area of perch and the net weight of individuals are improved.

The invention discloses a simple incubator, comprising a bubble cabinet with an upper cover. The upper part of the bubble cabinet is provided with a support grid plate. The moveable upper cover is provided with holes. A culture bottle passes through the hole of the upper cover and location holes of the support grid plate and extends to the lower part of the bubble cabinet. A bottle opening of the culture bottle is higher than the upper cover. A tampon is arranged in the bottle opening. The method comprises: in winter, placing the incubator on a place facing the sun in a room, detecting breeding condition of the drosophila melanogaster every other week, and changing fresh culture medium as needed; in summer, placing the incubator in a refrigerator preservation chamber at the temperature of 4 DEG C, taking the incubator out and placing in indoor environment for a day every other week, then detecting breeding condition of the drosophila melanogaster, and putting the incubator in a refrigerator to continue culture, at the time of 3-4 week, changing fresh culture medium as needed and subculturing. The bubble cabinet which is low in cost is used as the drosophila melanogaster incubator. The incubator is simple in structure and low in manufacturing cost. The incubator can ensure safe breeding of drosophila melanogaster population when being used in winter vacation or summer vacation, and the incubator satisfies requirements of drosophila melanogaster breed conservation breeding.

The invention discloses a method using the coupling of probiotic bacteria and virus for producing a medicine, which comprises the following steps: a, the protein of probiotic bacteria is taken; b, the protein of probiotic bacteria and the protein of virus are mixed at the mass ratio of 4-6:1, and EDC coupler of equal mass of the protein of probiotic bacteria and the protein of virus is added for coupling; c, the obtained coupling substance is prepared into immunogen for spare use; d, the immunogen is used for immunizing two times the heterogenic animal with the individual being bigger than the primitive virus host; e, after two-time immunization, the blood of the immunized heterogenic animal is collected for one time every other week to separate serum, HI test is used for checking the antibody titer of the virus and the probiotic bacteria, and when the antibody titer reaches 8Lg2, the blood is collected from the vein of the immunized heterogenic animal and serum is separated for spare use; f, one milliliter of the serum obtained in the step e is mixed with the suspension containing 2 billion probiotic bacteria to be stored at 4 DEG C after the 1-hour action at 37 DEG C.

The invention relates to an orchid disease prevention and cultivation method. The orchid disease prevention and cultivation method comprises the following steps of building a transparent greenhouse that can be closed and making a cultivation tank; firstly laying a layer of fermented cow manure in the cultivation tank, placing a perforated plastic plate on the cow manure layer, placing a layer of granular soil or sandy soil with a thickness of 5 cm on the plastic plate; transplanting orchid seedlings into the soil layer and watering every other week; and performing cow manure fermentation in the greenhouse. Compared with the prior art, the plastic plate of the orchid disease prevention and cultivation method can separate the soil layer and the cow manure layer, the water after watering infiltrates into the soil layer and penetrates the plastic plate to infiltrate into the cow manure layer, and fermentation of cow manure in the greenhouse can generate a certain amount of heat to keep thegreenhouse dry properly and can also provide water for the soil layer in order to enhance the ability of orchids to resist disease, prevent the appearance of spots on the orchids and make the plantsrobust.

The invention discloses a crop soilless seeding cultivation method. The crop soilless seeding cultivation method comprises the steps of carbon, carbon soil, vermiculite, perlite, coconut chaff, activated carbon and plant ash are acquired, mixed and stirred to obtain a cultivation medium; the cultivation medium is laid in seeding holes in multiple seeding trays; crop dibble seeding is conducted inthe cultivation medium in the seeding holes; the seeding trays with sowed seeds are placed in a seeding pond with a nutrient solution; the nutrient solution in the seeding pond is supplemented and / orreplaced every other week; when growing to a preset height, crop seedlings are transplanted. In this way, according to the crop soilless seeding cultivation method, the highly nutritional cultivationmedium is utilized for cultivating crops, the survival rate of the crops can be increased, the seedling recovery period of the crops is short, the crops grow fast, and thus the crop soilless seeding cultivation method can meet modern agricultural production requirements.