Method using coupling of probiotic bacteria and virus for producing medicine
A technology of probiotics and conjugates, which is applied in the field of drugs for the treatment of viral diseases and bacterial diseases, can solve the problems of high mortality, high cost, and high frequency of administration, and achieve the effect of reducing mortality and drug costs
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Embodiment 1
[0015] a. Pick a single colony (V ball) of purified Streptococcus faecalis from a sterile ultra-clean workbench and inoculate it into the sterilized MRS liquid medium, cultivate it at 37°C for 24 hours, and take 500ml of bacterial liquid (V ball) after the culture Ball) centrifuge (3000r / min), discard the supernatant after the end. Add 500ml of sterilized physiological saline to the centrifuge bucket again, blow the bacteria sludge to make a bacteria suspension, wash and centrifuge three times with 100ml of PBS buffer solution with a pH value of 8.0 (3000r / min), remove the supernatant and leave the precipitate, weigh Take the wet weight of the precipitate, that is, the amount of chicken-derived Streptococcus faecalis protein. The protein content of Newcastle disease virus was measured by spectrophotometer, the chicken-derived Streptococcus faecalis protein was mixed with the Newcastle disease virus protein at a mass ratio of 5:1, and then the chicken-derived Streptococcus faec...
experiment example 1
[0020] Get 100 SPF chickens, adopt intramuscular injection and oral route respectively to make its Newcastle disease virus and escherichia coli mixed infection, be a group by 50, be divided into two groups and administer respectively by prior art and embodiment 1 of the present invention, effect As in Table 1.
[0021] Table 1
[0022]
[0023] Results: The preparation of Example 1 was used as a drug for the treatment of mixed infection of Newcastle disease virus and Escherichia coli to act on sick chickens, and the cure rate was 82%.
Embodiment 2
[0025] a. Pick a single colony (V ball) of purified Streptococcus faecalis from a sterile ultra-clean workbench and inoculate it into the sterilized MRS liquid medium, cultivate it at 37°C for 24 hours, and take 500ml of bacterial liquid (V ball) after the culture Ball) centrifuge (3000r / min), discard the supernatant after the end. Add 500ml of sterilized physiological saline to the centrifuge bucket again, blow the bacteria sludge to make a bacteria suspension, wash and centrifuge three times with 100ml of PBS buffer solution with a pH value of 8.0 (3000r / min), remove the supernatant and leave the precipitate, weigh Take the wet weight of the precipitate, that is, the amount of chicken-derived Streptococcus faecalis protein. The protein content of infectious bronchitis virus was measured by spectrophotometer, the chicken source streptococcus faecalis protein was mixed with the infectious bronchitis virus protein in a mass ratio of 5:1, and then the chicken source streptococcu...
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