Method using coupling of probiotic bacteria and virus for producing medicine

A technology of probiotics and conjugates, which is applied in the field of drugs for the treatment of viral diseases and bacterial diseases, can solve the problems of high mortality, high cost, and high frequency of administration, and achieve the effect of reducing mortality and drug costs

Active Publication Date: 2015-02-18
DALIAN SANYI ANIMAL MEDICINE CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, for the mixed infection of Newcastle disease virus and Escherichia coli in chickens, it is necessary to inject intramuscular injection of Newcastle disease virus hyperimmune serum and antibiotics respectively. Not only the cost is high and the frequency of administration is high, but the stress response produced is large, and the cure rate is the highest. Up to 65%, the mortality rate of chickens after disease is high

Method used

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  • Method using coupling of probiotic bacteria and virus for producing medicine
  • Method using coupling of probiotic bacteria and virus for producing medicine
  • Method using coupling of probiotic bacteria and virus for producing medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0015] a. Pick a single colony (V ball) of purified Streptococcus faecalis from a sterile ultra-clean workbench and inoculate it into the sterilized MRS liquid medium, cultivate it at 37°C for 24 hours, and take 500ml of bacterial liquid (V ball) after the culture Ball) centrifuge (3000r / min), discard the supernatant after the end. Add 500ml of sterilized physiological saline to the centrifuge bucket again, blow the bacteria sludge to make a bacteria suspension, wash and centrifuge three times with 100ml of PBS buffer solution with a pH value of 8.0 (3000r / min), remove the supernatant and leave the precipitate, weigh Take the wet weight of the precipitate, that is, the amount of chicken-derived Streptococcus faecalis protein. The protein content of Newcastle disease virus was measured by spectrophotometer, the chicken-derived Streptococcus faecalis protein was mixed with the Newcastle disease virus protein at a mass ratio of 5:1, and then the chicken-derived Streptococcus faec...

experiment example 1

[0020] Get 100 SPF chickens, adopt intramuscular injection and oral route respectively to make its Newcastle disease virus and escherichia coli mixed infection, be a group by 50, be divided into two groups and administer respectively by prior art and embodiment 1 of the present invention, effect As in Table 1.

[0021] Table 1

[0022]

[0023] Results: The preparation of Example 1 was used as a drug for the treatment of mixed infection of Newcastle disease virus and Escherichia coli to act on sick chickens, and the cure rate was 82%.

Embodiment 2

[0025] a. Pick a single colony (V ball) of purified Streptococcus faecalis from a sterile ultra-clean workbench and inoculate it into the sterilized MRS liquid medium, cultivate it at 37°C for 24 hours, and take 500ml of bacterial liquid (V ball) after the culture Ball) centrifuge (3000r / min), discard the supernatant after the end. Add 500ml of sterilized physiological saline to the centrifuge bucket again, blow the bacteria sludge to make a bacteria suspension, wash and centrifuge three times with 100ml of PBS buffer solution with a pH value of 8.0 (3000r / min), remove the supernatant and leave the precipitate, weigh Take the wet weight of the precipitate, that is, the amount of chicken-derived Streptococcus faecalis protein. The protein content of infectious bronchitis virus was measured by spectrophotometer, the chicken source streptococcus faecalis protein was mixed with the infectious bronchitis virus protein in a mass ratio of 5:1, and then the chicken source streptococcu...

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Abstract

The invention discloses a method using the coupling of probiotic bacteria and virus for producing a medicine, which comprises the following steps: a, the protein of probiotic bacteria is taken; b, the protein of probiotic bacteria and the protein of virus are mixed at the mass ratio of 4-6:1, and EDC coupler of equal mass of the protein of probiotic bacteria and the protein of virus is added for coupling; c, the obtained coupling substance is prepared into immunogen for spare use; d, the immunogen is used for immunizing two times the heterogenic animal with the individual being bigger than the primitive virus host; e, after two-time immunization, the blood of the immunized heterogenic animal is collected for one time every other week to separate serum, HI test is used for checking the antibody titer of the virus and the probiotic bacteria, and when the antibody titer reaches 8Lg2, the blood is collected from the vein of the immunized heterogenic animal and serum is separated for spare use; f, one milliliter of the serum obtained in the step e is mixed with the suspension containing 2 billion probiotic bacteria to be stored at 4 DEG C after the 1-hour action at 37 DEG C.

Description

Technical field: [0001] The invention relates to a method for producing medicine for animals, in particular to a method for coupling probiotics and viruses to produce medicines that can simultaneously have antibacterial and immunoregulatory effects, and can simultaneously treat viral diseases and bacterial diseases. Background technique: [0002] At present, for mixed infection of viruses and bacteria in animals, drugs are usually administered separately and mostly by intramuscular injection. For example, for the mixed infection of Newcastle disease virus and Escherichia coli in chickens, it is necessary to inject intramuscular injection of Newcastle disease virus hyperimmune serum and antibiotics respectively. Not only the cost is high and the frequency of administration is high, but the stress response produced is large, and the cure rate is the highest. Reaching 65%, the mortality rate of the sick chicken is high. Invention content: [0003] The present invention aims ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61K39/395A61K35/74A61K35/16A61P31/04A61P31/12A61K35/741
Inventor 江国托李凤华王效禹
Owner DALIAN SANYI ANIMAL MEDICINE CO LTD
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