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Photobacterium phosphoreum growth promoter used for monitoring water body pollutants and application of photobacterium phosphoreum growth promoter

A technology of luminescent bacteria, growth promoters, applied in the fields of adding compounds to stimulate growth, microorganism-based methods, and microbial assays/tests, which can solve the problem of bacterial decline, unsuitable recovery and culture, and poor recovery culture conditions. temperature, pH, etc.

Active Publication Date: 2020-11-24
山东瑞泽检测评价技术服务有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, improper recovery and cultivation is an important reason for accelerated bacterial decline and death
Poor resuscitation culture conditions such as nutrient composition, temperature, pH value, etc. will not only induce cell death of the strain, but also induce the appearance of decayed cells. Once the decayed cells multiply rapidly, the number greatly exceeds that of normal cells, which will cause the strain to decline

Method used

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  • Photobacterium phosphoreum growth promoter used for monitoring water body pollutants and application of photobacterium phosphoreum growth promoter
  • Photobacterium phosphoreum growth promoter used for monitoring water body pollutants and application of photobacterium phosphoreum growth promoter
  • Photobacterium phosphoreum growth promoter used for monitoring water body pollutants and application of photobacterium phosphoreum growth promoter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The growth promoter of Photobacterium luminosus used for monitoring water pollutants, the specific components and concentrations are shown in Table 1:

[0040] Table 1

[0041] components Example 1 Example 2 Example 3 Example 4 Sodium chloride g / L 30 25 22 28 Ureag / L 2 3 2 4 Glucose g / L 5 7 6 8 Magnesium chloride g / L 4 3 4 3 Oxaloacetate g / L 1.5 1.1 1.3 1.6 Adenosine g / L 0.7 0.9 0.6 0.8 Yeast extract g / L 0.2 0.4 0.5 0.3 Formic acidml / L 20 15 18 19

[0042] The preparation method comprises the following steps: taking each component in sequence according to the concentration ratio, adding them into distilled water, stirring evenly, and adjusting the pH to 7.0-7.5 to obtain the product.

Embodiment 2

[0048] Application example of growth promoter of Photobacterium luminosa.

[0049] The freeze-dried powder is commercially available Lyophilized Bacillus Brightensis (ATCC11040), 1g / tube; self-made freeze-dried powder can also be used.

[0050] Preparation method of homemade freeze-dried powder:

[0051] Under sterile conditions of the lyoprotectant, the solution of Photobacterium lumina (concentration of 1×10 9cfu / ml) and lyoprotectant (parts by weight: skimmed milk powder 10%, sucrose 8%, sodium glutamate 1%, solvent is sterile water) mixed according to the volume ratio of 1:1, packed in vials Cool down to -70°C within 60 minutes, freeze for 12 hours, then place at -40°C, vacuum freeze-dry to obtain a freeze-dried powder, and finally store at -20°C.

[0052] Methods for the recovery and expansion of Photobacteria luminosus:

[0053] Take out the lyophilized powder, put it in a 38°C water bath to thaw immediately, shake it for 60s, after it is completely thawed, add it to ...

Embodiment 3

[0055] Effects of example 1-4 growth promoters on bacterial strain proliferation and luminous intensity.

[0056] Detection method: Spectrophotometer method (OD600) is used for the detection of bacteria concentration; DXY-3 intelligent biological toxicity tester is used for detection of luminous intensity of bright photobacteria.

[0057] Test procedure: Referring to Example 2, commercially available freeze-dried powder was used. Such as figure 1 As shown, the growth promoters of Examples 1-4 can realize the rapid recovery and proliferation of bright photobacteria within 6 hours, which is beneficial to save detection time, and obtain a large amount of bacteria liquid with stable luminous efficiency in a short time, expanding the detection scale , reducing business costs.

[0058] Luminous intensity comparison:

[0059] Conventional method: Take out the freeze-dried powder, immediately place it in a 38°C water bath to thaw, shake for 60s, then add an appropriate amount of 3%...

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Abstract

The invention belongs to the technical field of biology and discloses a photobacterium phosphoreum growth promoter used for monitoring water body pollutants. The photobacterium phosphoreum growth promoter comprises components as follows: sodium chloride, urea, glucose, magnesium chloride, oxaloacetic acid, adenosine, yeast extract and formic acid. The photobacterium phosphoreum growth promoter used for monitoring the water body pollutants can rapidly recover and enlarge cultivation, thereby saving monitoring time and reducing burdens of enterprises.

Description

technical field [0001] The invention belongs to the technical field of biological environmental protection, and in particular relates to a bright photobacteria growth promoter for monitoring water body pollutants and an application thereof. Background technique [0002] Luminescent bacteria have been used in various fields as early as the end of the last century. For the first time, foreign scientists isolated and screened luminescent bacteria that are sensitive to the environment and used them to detect water biological toxicity. In recent years, this method has gradually become a simple and rapid biological toxicity detection method. [0003] Photobacillus luminosa is a luminescent bacterium that is widely used. The general application steps are: purchase of lyophilized powder of strains-resuscitation-dilution-contaminant monitoring. However, the above-mentioned application method will have the problem of decreased survival rate and luminous efficiency of the strain after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12Q1/02C12R1/01
CPCC12N1/20C12N1/38C12Q1/02C12Q2304/60
Inventor 汪怡杜欣
Owner 山东瑞泽检测评价技术服务有限公司
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