Tissue culture rapid propagation method of callicarpa nudiflora

A technique of nude flower purple pearl and tissue culture, which is applied in the field of agricultural biology, can solve the problems of being unable to meet the needs of production, the small coefficient of division breeding seedlings, and the low seed setting rate, etc., and achieves low cost, fast multiplication speed, and method easy-to-use effects

Inactive Publication Date: 2013-01-16
INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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Problems solved by technology

The seed setting rate and seedling emergence rate of Zizhu nudiflora are low, and the coefficient of division breeding seedlings is very small, which cannot meet the needs of production

Method used

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  • Tissue culture rapid propagation method of callicarpa nudiflora

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Embodiment Construction

[0016] The present invention a kind of method for fast propagation of nudibranch purple pearl by tissue culture, comprises the following steps:

[0017] (1) Selection of explants

[0018] Use cuttings to propagate a small amount of nudibranchs, and spray fungicides regularly. When the new branches grow to 15-25cm, take two or three sections of stems, cut them off, put them in a sterilized airtight glass bottle, and seal them. The workbench is soaked in 75% alcohol for 15-30 seconds, then sterilized with 0.1% mercury liter for 5-7 minutes, and finally rinsed with sterile water for 5-7 times, and used as explants for tissue culture and rapid propagation of Aurora nudiflora;

[0019] (2), inoculation culture

[0020] Cut off the 1-2 cm long single-node stem section of the sterilized Aurora nudiflora, and place it on the inoculation medium that promotes the germination and growth of the lateral buds of the stem section. The inoculation medium is WPM basic medium with an additiona...

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Abstract

The invention discloses a tissue culture rapid propagation method of callicarpa nudiflora. The method performs control on the explant collection, disinfection method, differentiation, multiplication, screening of a rooting medium, selection of a transplanting matrix, environmental conditions in the culture process and the like. By adopting the methods of explant selection, inoculated culture, propagation expanding culture, rooting culture and hardening-seedling transplanting, a set of complete callicarpa nudiflora tissue culture rapid propagation technology system is established, so that the differentiation rate reaches 100%, the monthly multiplication coefficient reaches 4.95, the rooting rate reaches 93.7%, and the transplanting survival rate exceeds 90%. The method disclosed by the invention is simple and easy to implement, simple in process and high in efficiency, effectively suppresses the pollution and browning of the callicarpa nudiflora explant, improves the differentiation rate of the tissue culture seedling, realizes fast multiplication, high rooting rate, high survival rate and low cost, and provides a reliable technical means for large-scale production of callicarpa nudiflora seedlings.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a method for rapidly propagating the nude flower purple pearl by tissue culture. Background technique [0002] Callicarpa nudiflora (Callicarpa nudiflora) is an important medicinal plant. Its roots and leaves are used medicinally. tract bleeding, tooth extraction bleeding, bruises, rheumatic arthralgia embolism. The seed setting rate and seedling emergence rate of Zizhu nudiflora are low, and the coefficient of division breeding seedlings is very small, which cannot meet the needs of production. Contents of the invention [0003] The purpose of the present invention is to provide a simple, simple procedure, high efficacy, effectively inhibit the pollution and browning of the explants of the nude flower, improve the differentiation rate of the tissue culture seedlings, the proliferation speed is fast, and the rooting The method has the advantages of hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王效宁朱红林陈健晓孟卫东齐兰
Owner INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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