Tissue-culture propagation method of cold-resistant Chinese rose

A tissue culture and rose technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow ramet propagation, low seed propagation rate, large variation, etc. The effect of industrialization demand and meeting market demand

Active Publication Date: 2018-03-16
HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES +1
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of cold-resistant rose tissue culture breeding method, to solve the problem of low seed reproduction rate, large variation, slow propagation

Method used

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  • Tissue-culture propagation method of cold-resistant Chinese rose
  • Tissue-culture propagation method of cold-resistant Chinese rose
  • Tissue-culture propagation method of cold-resistant Chinese rose

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[0022] The present invention is a cold-resistant rose tissue culture and breeding method, which includes the following steps

[0023] 1) Screening of explants: In spring, select the buds germinated from healthy plants, and cut the branches with new buds with branch shears as explants;

[0024] 2) Disinfection of explants: Cut the branches with buds in step 1) into small sections, each with 1-3 buds, and place them in a 200mL beaker. After the cup is covered with gauze, it is washed with tap water. Add appropriate amount of detergent and keep it in the tap water for 30-40 minutes. Use filter paper to absorb the water; add 1‰ mercury chloride solution to the beaker to completely soak the explant material, add 2 drops of Tween 20(Tween20) is made into disinfectant, shake it gently, pour out all the disinfectant in the beaker after 6-8 minutes, pour sterile water into the beaker to rinse the explant material 4 times, use less sterile water first Multi-principle. After cleaning until t...

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Abstract

The invention relates to a tissue-culture propagation method of cold-resistant Chinese rose and solves the propagation problems of slow propagation and low multiplication coefficient of the cold-resistant Chinese rose. The method includes the steps of firstly, screening explants; secondly, sterilizing the explants; thirdly, performing primary culture; fourthly, performing multiplication culture; fifthly, performing strong-seedling culture; sixthly, performing rooting culture; seventhly, transplanting and performing acclimation. The method is characterized in that newly grown top buds or lateral buds of plants are used as the explants, MS is used as the basic culture medium, naphthylacetic acid (NAA), 6-benzylamino purine (6BA) and indolebutyric acid (IBA) are utilized to perform hormonal regulation, and accordingly the regeneration system of the cold-resistant Chinese rose is built. The method has the advantages that one multiplication subculture cycle of the method is 4-5 weeks, multiplication times is controlled to be 6-8 times, the method is unaffected by seasons, seedling propagation coefficient can be increased greatly, a scientific and effective propagation method is providedfor the cultivation and application of the cold-resistance Chinese rose, production requirements are satisfied, industrial propagation can be performed, and reference can be provided for the propagation of other cold-resistant Chinese rose varieties.

Description

technical field [0001] The invention relates to a method for tissue culture and breeding of cold-resistant roses. Background technique [0002] The invention relates to a cold-resistant rose, which is a perennial woody flower of the family Rosaceae (Rosaceae) and belongs to the genus Rosa (Rosa L.), and is a thorny shrub. Leaves alternate, odd-pinnate compound leaves. The flowers are solitary or in panicles, with many petals, rich and bright colors, and a long flowering period. In addition to being used as cut flowers, it is also an important material for flower beds, flower borders, foundation planting and flower hedges. It is often found in lawns, gardens, park green spaces, etc. Planting is widely used in landscaping. [0003] However, due to the cold and long winter in Northeast China and the short growing season in summer, there are few types of plants available for urban greening, and the cost of greening is relatively high. Especially in Heilongjiang Province, there...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 曲彦婷张兴唐焕伟陈菲李黎韩辉熊燕宫伟何静张洪运李虹
Owner HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES
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