Tissue-culture propagation method of cold-resistant Chinese rose
A tissue culture and rose technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow ramet propagation, low seed propagation rate, large variation, etc. The effect of industrialization demand and meeting market demand
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[0022] The present invention is a kind of cold-resistant Chinese rose tissue culture breeding method, comprises the following several steps
[0023] 1) explant screening: in spring, select the tender buds that healthy plants germinate, and use branch scissors to cut off the branches with new buds as explants;
[0024] 2) explant disinfection: step 1) the branch with tender bud is cut into small sections, each section has 1-3 buds, is placed in a 200mL beaker, after the mouth of the cup is covered with gauze, after using tap water to wash, Add appropriate amount of detergent and keep rinsing in tap water for 30-40 minutes, blot the water with filter paper; add mercuric chloride solution with a mass percentage concentration of 1‰ into the beaker to completely soak the explant material, add 2 drops of Tween 20 (Tween20) to make a disinfectant solution, shake it gently, pour out all the disinfectant solution in the beaker after 6-8 minutes, pour sterile water into the beaker to ri...
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