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Application of universal calcium preparation in neural stem cell differentiation regulation

A technology of neural stem cells and calcium preparations, which is applied in the fields of biomaterials and medical care and health, can solve the problems of slow differentiation of neural stem cells, increase the economic burden of patients, and accelerate neural stem cells, so as to improve repair efficiency and avoid neural scars. wide effect

Active Publication Date: 2021-08-20
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Research is being done in various fields to find treatments for neurodegenerative diseases, but conventional surgical and drug treatments have had little effect, and there is an urgent need to target neurodegenerative Research on basic treatment of disease etiology
[0005] In recent years, neural stem cell transplantation has gradually become the most potential treatment method. However, neural stem cell transplantation faces problems such as slow neural stem cell differentiation and uncertain direction, and accelerates neural stem cell differentiation. It is imminent to regulate the process and direction of neural stem cell differentiation
Traditional methods mainly rely on a large number of biological reagents to accelerate the differentiation of neural stem cells and regulate the direction of neural stem cell differentiation. However, biological reagents fail quickly, are expensive, complex in composition, and difficult in quality inspection, which increases the economic burden on patients and also brings many problems. Uncertainty and even risk factor

Method used

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  • Application of universal calcium preparation in neural stem cell differentiation regulation
  • Application of universal calcium preparation in neural stem cell differentiation regulation
  • Application of universal calcium preparation in neural stem cell differentiation regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Preparation of hydroxyapatite nanorods:

[0055] 1) Dissolve 0.6g of octadecylamine in 7ml of oleic acid, heat at 80°C for 30min, and add 20mL of ethanol under vigorous stirring;

[0056] 2) Add 1.4mL of 0.2M NaF, 7mL of 0.168M NaF 3 PO 4 , stir for 5min;

[0057] 3) Then add 7mL of 0.2M Ca(NO 3 ) 2 solution and stirred for 1min to obtain a mixed solution,

[0058] 4) The mixed solution was heated and reacted at 180° C. for 12 hours, and then naturally cooled to room temperature;

[0059] 5) Disperse the product in cyclohexane, centrifuge at 10,000 rpm for 10 min, wash with cyclohexane and ethanol three times, and finally disperse in ethanol for later use.

[0060] The TEM figure of the HAp nanorods that this embodiment makes is shown in figure 1 It can be seen from the figure that the HAp nanorods are about 100nm long and about 20nm wide.

[0061] The application of hydroxyapatite nanorods in the regulation of neural stem cell differentiation is as follows:

...

Embodiment 2

[0067] The preparation of hydroxyapatite nanorods is the same as in Example 1.

[0068] The application of hydroxyapatite nanorods in regulating the differentiation of neural stem cells is the same as that in Example 1, except that:

[0069] The concentration of hydroxyapatite nanorods in the system was 400 μg / ml.

Embodiment 3

[0071] The preparation of hydroxyapatite nanorods is the same as in Example 1.

[0072] The application of hydroxyapatite nanorods in regulating the differentiation of neural stem cells is the same as that in Example 1, except that:

[0073] The concentration of hydroxyapatite nanorods in the system was 50 μg / ml.

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Abstract

The invention relates to application of a universal calcium preparation in neural stem cell differentiation regulation. The method comprises the following steps that neural stem cells are inoculated into a neural stem cell differentiation culture medium; a universal calcium preparation is added into a culture system for neural stem cell culture and endocytosis, wherein the universal calcium preparation is hydroxyapatite nanorods or calcium phosphate nanoparticles, and neural stem cells modified by the calcium preparation are obtained; and the neural stem cells modified by the calcium preparation are transplanted for relieving and treating neurodegenerative diseases. The neural stem cells modified by the calcium preparation disclosed by the invention have a relatively high differentiation speed and a relatively obvious neuronal directional differentiation trend, and can generate more mature neurons in a shorter time, so that the nerve tissue repair efficiency is improved to the greatest extent, and the generation of nerve scars is avoided.

Description

technical field [0001] The invention relates to the application of a general-purpose calcium preparation in regulating the differentiation of neural stem cells, and belongs to the technical field of biological materials and medical care and health. Background technique [0002] With the aging population, people in modern society suffer from neurodegenerative diseases, the most common neurodegenerative diseases include Alzheimer's disease (AD) and Parkinson's disease (PD). Based on what is known so far, damage to neuronal cells in specific locations in the brain, such as the hippocampus or substantia nigra, produces defective neural networks in reduced numbers of neuronal cells, which in turn leads to neurodegenerative diseases of various symptoms. AD is a common and incurable degenerative disease of the central nervous system. It is characterized by low cognitive ability, brain control reasoning and continued deterioration of function of memory areas that cannot be changed...

Claims

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Application Information

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IPC IPC(8): A61K35/30A61K33/42C12N5/0797A61P25/00A61P25/28
CPCA61K35/30C12N5/0623A61P25/28A61P25/00A61K33/42C12N2500/14C12N2500/05A61K2300/00
Inventor 桑元华郝敏何建龙段佳志夏鹤陈鑫王书华刘宏
Owner SHANDONG UNIV
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