T cell serum-free medium and using method thereof

Abstract

The invention belongs to the field of culture mediums, and in particular relates to a T cell serum-free medium and a using method thereof. The T cell serum-free medium consists of a basal culture medium applied to growth culture of cells, as well as the following addition ingredients: ethanolamine, copper sulfate, ferric nitrate, zinc sulfate, sodium selenite, sodium pyruvate, insulin, transferrin, glutamine, serum albumin, thioglycerol and L-vitamin C. The T cell serum-free medium provided by the invention breaks through shortcomings of the prior art, and according to the T cell serum-free medium, the entire medium system is free from the introduction of non-human proteins, and the T cells in the blood sample, which is safe and reliable in source, undergo efficient selective amplification; and therefore, higher clinical application and scientific research values are achieved.

Description

technical field [0001] The invention belongs to the field of culture medium, and in particular relates to a T cell serum-free culture medium and a use method thereof. Background technique [0002] T cells are one of the main components of lymphocytes. They have a variety of biological functions, such as directly killing target cells, assisting other lymphocytes to function, responding to specific antigens or mitogens, and producing cytokines. One of the body's main immune cells that fight off infection and prevent tumors from forming. Enrichment, activation, and expansion of such immune cells in vitro can be used for reinfusion to treat various diseases, including malignant tumors, infections, autoimmune diseases, etc. In this process, it is necessary to use a T cell that can support the survival of T cells in vitro and amplified media. [0003] At present, RPMI-1640 or DMEM medium is mostly used for T cell culture, but the nutrients required for the survival and expansion...

AI Technical Summary

Problems solved by technology

These culture media are more or less added with animal-derived or human blood-derived components. Although there are strict testing and virus inactivation treatments, there are still certain potential safety hazards.

[0005] Therefore, the current T cell culture medium has major problems such as low culture efficiency, low cell activity, follow-up test interference, and animal serum protein residues.

Images