2results about How to "Enhance specific binding" patented technology

The present application relates to the technical field of nanobody, in particular to anti-FcRH5 nanobody and its application. The present application provides a kind of anti-FcRH5 nanobody with high affinity and specificity, which is based on the single domain heavy chain variable region structure of camelid origin, with the advantages of small molecular weight, high stability, strong tissue penetration, easy to be engineered and the like. The nanobody specifically recognizes and binds to the target point highly expressed and stably expressed on the surface of multiple myeloma cells through its complementarity determining region. The nanobody of the present application can be used as a core recognition element to construct a variety of therapeutic or diagnostic tools such as chimeric antigen receptor T cells, bispecific antibodies, antibody drug conjugates or immunodetection probes. The technical scheme can solve the technical problem that there is a lack of high-quality, functionally clear anti-FcRH5 antibody in the prior art. The nanobody of the present application has significant clinical transformation and industrialization advantages, and promotes the treatment of multiple myeloma to multiple target points.

The application relates to the field of biology, and particularly discloses a PCR amplification system and a PCR nucleic acid detection method; the PCR amplification system comprises a PCR additive, the PCR additive comprises tetramethylene sulfoxide modified gold nanoparticles, and the particle size of the tetramethylene sulfoxide modified gold nanoparticles is 0.5-1.1 nm; and the amplification method comprises the following steps: S1, configuring a PCR reaction system, adding template DNA into the prepared reaction system to prepare a mixed solution; S2, placing the mixed solution in a high-temperature reaction zone and a low-temperature reaction zone to perform 30-45 amplification cycles, the temperature of the high-temperature reaction zone is one fixed temperature or a temperature interval in the range of 94 DEG C-100 DEG C, and the temperature of the low-temperature reaction zone is one fixed temperature or a temperature interval in the range of 50 DEG C-65 DEG C. The method has the advantages of shortening the time of the PCR amplification process.