1results about How to "Enhance specific binding" patented technology

The application relates to the field of biology, and particularly discloses a PCR amplification system and a PCR nucleic acid detection method; the PCR amplification system comprises a PCR additive, the PCR additive comprises tetramethylene sulfoxide modified gold nanoparticles, and the particle size of the tetramethylene sulfoxide modified gold nanoparticles is 0.5-1.1 nm; and the amplification method comprises the following steps: S1, configuring a PCR reaction system, adding template DNA into the prepared reaction system to prepare a mixed solution; S2, placing the mixed solution in a high-temperature reaction zone and a low-temperature reaction zone to perform 30-45 amplification cycles, the temperature of the high-temperature reaction zone is one fixed temperature or a temperature interval in the range of 94 DEG C-100 DEG C, and the temperature of the low-temperature reaction zone is one fixed temperature or a temperature interval in the range of 50 DEG C-65 DEG C. The method has the advantages of shortening the time of the PCR amplification process.