Two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH) and preparation method of kit

A fluorescent immunochromatography and quantitative detection technology, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of complicated process, reduced sensitivity, and high price, and achieve the effects of high sensitivity, good stability, and narrow emission spectrum

Inactive Publication Date: 2018-01-23
DEMAIJI BIOTECH BEIJING
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Problems solved by technology

[0009] At present, the immunological methods commonly used in clinical AMH detection include enzyme-linked immunoassay, chemiluminescence, and electrochemiluminescence; because the enzyme-linked immunoassay requires enzyme labeling, the process is complicated, and professional personnel are required to operate, and the American Union immunoassay Liquid reagents require low-temperature refrigerated transportation, which brings great inconvenience

Method used

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  • Two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH) and preparation method of kit
  • Two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH) and preparation method of kit
  • Two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH) and preparation method of kit

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Embodiment 1

[0045] Example 1: Covalently cross-linked antibodies are modified with fluorescent dyes, and the Wash immunochromatographic technique is used to counter the quantitative detection of Mullerian hormone (AMH)

[0046] 1) Coupling of fluorescent dye and antibody

[0047] Mix the fluorescent dye with the emission wavelength of 365nm and 1mg / ml anti-Müllerian hormone (AMH) labeled monoclonal antibody, react at room temperature for 3h, add 1mol / L hydroxylamine hydrochloride to stop the reaction, and use a chromatographic column or a chromatographic column to separate and purify. A fluorescent dye-modified anti-Müllerian hormone (AMH) monoclonal antibody was obtained. In the same way, rabbit IgG (quality control molecule) modified with fluorescent dye is obtained. The fluorescence emission wavelength of the fluorescent dye of the modified anti-Müllerian hormone (AMH) antibody and the fluorescence emission wavelength of the fluorescent dye of the modified rabbit IgG are both 365 nm.

[004...

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Abstract

The invention discloses a two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH), which utilizes fluorescent dye as a maker. The two-photon fluorescence immunochromatography kit, realizing fluorescence immunochromatography quantitative determination, has the advantages of good stability, wide linear range, good specificity, high sensitivity,high quantitative accuracy and easy and quick operation, can be applied to detection of whole blood samples, serum samples and plasma samples simultaneously, and is applicable to medical treatment ofhospital at different levels and family practice.

Description

Technical field [0001] The invention relates to the field of cancer clinical immunodiagnostic kits in the field of medical testing, in particular to a two-photon fluorescence immunochromatographic kit for quantitative detection of anti-Müllerian hormone (AMH). Background technique [0002] In the past 20 years, assisted reproductive technology (ART) has continuously improved, and the success rate has increased significantly, but ovarian reserve is still the main factor affecting the success rate of in vitro fertilization and embryo transfer (IVF-ET) factor. A large number of studies have found that anti-Mullerian hormone (AMH) can more accurately assess the reserve function of the ovary and predict the responsiveness of the ovaries to gonadotropins than traditional indicators. It has even found that the ability of follicles to synthesize AMH is related to the ability of the egg and the egg. The quality of embryos is related. It is believed that AMH can predict the outcome of ART...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558
Inventor 李道锋潘文东朱建峰贺率
Owner DEMAIJI BIOTECH BEIJING
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