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38results about How to "Reduce the impact of access" patented technology

Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay

ActiveCN102520165ASensitive quantitative detection fastRealize detectionMaterial analysisCritical illnessLinear range
The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.
Owner:BEIJING KANGMEI TIANHONG BIOTECH

Fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and reagent kit thereof

ActiveCN102539785ASolve the backgroundSolve the signal indistinguishableBiological testingFluorescence/phosphorescenceBasic levelQuantum dot
The invention discloses a fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and a reagent kit thereof. The fluorescent immunochromatography method for the whole quantitative detection of the C-reactive protein (CRP) utilizes excellent fluorescent characteristics of quantum dots, and combines double-color marking technology and immunochromatography technology to achieve fluorescent quantitative detection on the basis of optimizing each constituent elements of test paper. Compared with a conventional colloidal gold immunochromatography method, the fluorescent immunochromatography method for the whole quantitative detection of the CRP has the advantages of being good in stability, low in non-specificity, high in flexibility, wide in linear range and accurate in quantifying. The reagent kit of the fluorescent immunochromatography method can perform the whole quantifying and can simultaneously predict and evaluate infectious diseases, antibiotic effects and cardiovascular and cerebrovascular diseases. The fluorescent immunochromatography method for the whole quantitative detection of the CRP and the reagent kit of the fluorescent immunochromatography method are suitable for various-level hospitals, and particularly contribute to wide popularization in basic-level hospitals and clinics.
Owner:SHENZHEN KANGMEI BIOTECH

Fluorescence immunochromatographic assay method for quantitatively detecting heart fatty acid binding protein and kit for quantitatively detecting same

ActiveCN102520194ASolve the backgroundSolve the signal indistinguishableBiological testingBlood plasmaBiology
The invention discloses a fluorescence immunochromatographic assay method for quantitatively detecting hFABP (heart fatty acid binding protein) and a kit for quantitatively detecting the same. The fluorescence immunochromatographic assay method for quantitatively detecting the hFABP realizes quantitative fluorescence detection on the basis of optimizing components of a test strip by the aid of excellent fluorescent characteristics of quantum dots and by means of combining bicolor labeling technique and immunochromatographic assay. Compared with a conventional colloidal gold immunochromatographic assay method, the fluorescence immunochromatographic assay method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The kit is used for quantitatively detecting the hFABP, can be used for simultaneously detecting whole blood, blood serum and plasma samples, serves as a simple, accurate, specific and inexpensive detecting tool for early screening and prognosis evaluation of acute myocardial infarction, is applicable to hospitals at all levels, and is particularly beneficial to wide popularization in primary hospitals and clinics.
Owner:SHENZHEN KANGMEI BIOTECH

Immunofiltration assay fluorescent quantitative detection method based on high-sensitivity quantum dot

The invention discloses an immunofiltration assay fluorescent quantitative detection method based on a high-sensitivity quantum dot; the immunofiltration assay fluorescent quantitative detection method comprises the following steps of: constructing a fluorescence immunofiltration array device by using the excellent fluorescent characteristic of the quantum dot in combination of a quantum dot fluorescence labeling technology and an immunofiltration array technology on the basis of optimizing constituent parts for the immunofiltration assay; and after immunofiltration array, detecting the strenght of fluorescent signals of the quantum dot and a quality control dot by using a fluorescence quantometer, correcting the fluorescence strenght of the quantum pot by using the quality control dot, and further realizing the quantitative detection of a tested object according to a standard curve obtained by using the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and high in sensitiveness. Comapred with the conventional collodial gold immunofiltration array method, the immunofiltration assay fluorescent quantitative detection method has the advantages of good labeling stability, low non-specificity, high sensitivity, wide linear range and accurate quantification. The method is suitable for samples such as serums, urine, spittle, excrement and the like and can be applicable to the detection of serious illness, poisons, food safety and the like.
Owner:BEIJING KANGMEI TIANHONG BIOTECH

Low-orbit satellite Internet of Things channel resource dynamic allocation method based on SDN

ActiveCN112272412ASolve problems such as centralized management difficultiesMaximize access success rateNetwork topologiesTelecommunicationsGeoweb
The invention provides a low-orbit satellite Internet of Things channel resource dynamic allocation method based on SDN. In an access matching flow table calculation step, the distance between the sub-satellite point position of each satellite and the geographic grid where a satellite Internet of Things terminal is located is calculated through a ground controller to judge which satellite should be selected by the terminal for access; in a satellite channel resource dynamic allocation step, the QoS grade, the quantity, the minimum access success rate limit and the satellite available resourcesof the access terminal service are calculated through a satellite controller, and the channel resources are dynamically divided; and in an access control assisted random access step, competitive random access is carried out according to the channel resources allocated by the terminal. According to the method, on the basis of an SDN architecture, channel resources are allocated through cooperativeoptimization of the ground controller and the satellite controller, so that the access success rate of high-priority services is maximized and the access performance of services with different priorities is guaranteed while the success rate of accessing channels of services with medium and low priorities is not lower than a specified threshold value.
Owner:NANJING UNIV OF POSTS & TELECOMM

Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.
Owner:BEIJING KANGMEI TIANHONG BIOTECH

Immunofiltration assay fluorescent quantitative detection method based on high-sensitivity quantum dot

The invention discloses an immunofiltration assay fluorescent quantitative detection method based on a high-sensitivity quantum dot; the immunofiltration assay fluorescent quantitative detection method comprises the following steps of: constructing a fluorescence immunofiltration array device by using the excellent fluorescent characteristic of the quantum dot in combination of a quantum dot fluorescence labeling technology and an immunofiltration array technology on the basis of optimizing constituent parts for the immunofiltration assay; and after immunofiltration array, detecting the strenght of fluorescent signals of the quantum dot and a quality control dot by using a fluorescence quantometer, correcting the fluorescence strenght of the quantum pot by using the quality control dot, and further realizing the quantitative detection of a tested object according to a standard curve obtained by using the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and high in sensitiveness. Comapred with the conventional collodial gold immunofiltration array method, the immunofiltration assay fluorescent quantitative detection method has the advantages of good labeling stability, low non-specificity, high sensitivity, wide linear range and accurate quantification. The method is suitable for samples such as serums, urine, spittle, excrement and the like and can be applicable to the detection of serious illness, poisons, food safety and the like.
Owner:BEIJING KANGMEI TIANHONG BIOTECH

System for quantitatively detecting heavy metal cadmium and preparation method thereof

The invention provides a system for quantitatively detecting heavy metal cadmium, which comprises a buckle, a fluorescence immunochromatography test strip and a sample dilution buffer solution, wherein the buckle is of an outer shell structure of the fluorescence immunochromatography test strip and is provided with a sample adding hole and an observation window; the fluorescence immunochromatography test strip comprises a sample pad, a marking pad, a chromatography membrane, a water absorption pad and a bottom plate; a fluorescent dye modified heavy metal cadmium specific antibody and a fluorescent dye modified quality control molecule are fixed on the marking pad at the same time; a heavy metal cadmium chelating agent hapten is fixed on the quantitative detection line; the hapten is specifically combined with a fluorescent dye modified heavy metal cadmium specific antibody fixed on the marking pad; biomolecules capable of being specifically combined with the quality control moleculesare fixed on the quality control line; and a to-be-detected sample is treated with the sample dilution buffer solution before detection. In order to detect the heavy metal cadmium in the sample, treatment by using the sample dilution buffer solution is carried out before the to-be-detected sample is detected, and the sample dilution comprises a chelating agent which is combined with the heavy metal cadmium to form a heavy metal cadmium chelating agent hapten; if the sample dilution buffer solution is not used for treating a sample, the sample is directly detected, the heavy metal cadmium in the sample cannot be detected, and the existence of the sample dilution buffer solution is crucial to the detection of the cadmium in the sample.
Owner:北京大弘生物技术有限公司

Fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and reagent kit thereof

The invention discloses a fluorescent immunochromatography method for whole quantitative detection of C-reactive protein and a reagent kit thereof. The fluorescent immunochromatography method for the whole quantitative detection of the C-reactive protein (CRP) utilizes excellent fluorescent characteristics of quantum dots, and combines double-color marking technology and immunochromatography technology to achieve fluorescent quantitative detection on the basis of optimizing each constituent elements of test paper. Compared with a conventional colloidal gold immunochromatography method, the fluorescent immunochromatography method for the whole quantitative detection of the CRP has the advantages of being good in stability, low in non-specificity, high in flexibility, wide in linear range and accurate in quantifying. The reagent kit of the fluorescent immunochromatography method can perform the whole quantifying and can simultaneously predict and evaluate infectious diseases, antibiotic effects and cardiovascular and cerebrovascular diseases. The fluorescent immunochromatography method for the whole quantitative detection of the CRP and the reagent kit of the fluorescent immunochromatography method are suitable for various-level hospitals, and particularly contribute to wide popularization in basic-level hospitals and clinics.
Owner:SHENZHEN KANGMEI BIOTECH

Domain name resolution service system with intelligent buffer and service method thereof

The invention relates to a domain name resolution service system with an intelligent buffer and a service method thereof in the technical field of network service, wherein the system comprises a domain name resolution module, an intelligent buffer module, an intelligent buffer updating module, a domain name resolution mistake judgment module and a domain name resolution mistake processing module;the method comprises the update of an iterative inquiry and the intelligent buffer, the judgment of a domain name resolution mistake and the processing of the domain name resolution mistake. When a fault of a domain name authorization server at a client end occurs, a correctly resolved result of the last time of a domain name influenced by the fault can be obtained to access a website or service corresponding to the domain name, so that the network browse of a user can not be influenced by the fault of the domain name authorization server; a safeguard can be supplied for the appointed important domain name and key service, the reliability of domain name resolution service is improved, and the influence of the fault of the domain name authorization server on website or service access is greatly reduced; and the invention is transparent for the user, and the software and the configuration of the client end need not to be modified.
Owner:SHANGHAI JIAOTONG UNIV
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