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Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay

A fluorescence immunochromatography, quantitative detection technology, applied in the direction of measurement devices, analytical materials, instruments, etc., can solve the problems of low sensitivity, difficult to distinguish background and signal, unclear fluorescence quantitative method, etc., to improve sensitivity and improve detection sensitivity. Effect

Active Publication Date: 2014-07-30
BEIJING KANGMEI TIANHONG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The technical problem to be solved by the present invention is to provide a quantum dot fluorescent immunochromatography method for the problems of indistinguishable background and signal, low sensitivity, and unclear fluorescence quantitative method in the existing immunochromatography technology, so as to realize the analysis of samples Highly sensitive semi-quantitative and quantitative detection of substances

Method used

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  • Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay
  • Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Example 1: Double-antibody sandwich determination of human chorionic gonadotropin (hCG)

[0072] (1) Synthesis and modification of quantum dots

[0073] Take 0.2375g of selenium powder, 2.60mL of octadecene and 1.57mL of tri-n-octylphosphine, add them in turn to a 25ml small reagent bottle, heat the mixed liquid in the bottle and shake repeatedly until the selenium powder is completely dissolved, and then the selenium precursor is obtained. Another 0.0368g of cadmium oxide, 0.342g of stearic acid and 3.8ml of octadecene were added to the three-necked flask in sequence, and heated under the protection of nitrogen until the cadmium oxide was completely dissolved. Lower the temperature to cool the solution to solidify. Take 2.25g of octadecylamine and 0.95g of trioctylphosphine oxide, put them into a three-necked flask, and heat to melt the solid, continue to heat to 280°C, inject 4.2mL of selenium precursor, and raise the temperature to 240°C to make the CdSe quantum dot...

Embodiment 2

[0086] Embodiment 2: Competitive method is measured organophosphorus

[0087] (1) Quantum dot synthesis and modification

[0088] For the synthesis of CdTe quantum dots, add absolute ethanol and primary water to the reactor containing appropriate amount of Te powder and sodium borohydride in the ratio of 2:1 by volume, and react in a water bath at 60°C under nitrogen gas until black Te The powder is completely gone. Add a slight excess of 0.5mol / L H 2 SO 4 , yielding H 2 Te is absorbed by NaOH solution to obtain NaHTe aqueous solution.

[0089] CdCl under nitrogen 2 2.5 H 2 O was dissolved in 100mL ultrapure water, then added mercaptopropionic acid, and 0.5mol / L NaOH solution was added dropwise under stirring to adjust the pH of the solution to 11.2, and finally 0.1mmol NaHTe solution was injected, wherein Cd: Te: mercaptopropionic acid The ratio is 1.0:0.5:2.4. The resulting mixed solution was heated to reflux at 100° C. under nitrogen to obtain CdTe quantum dots, whi...

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Abstract

The invention discloses a method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay. The method includes: building a fluorescence immunochromatographic assay test strip on the basis of optimizing the structure of the test strip and components by the aid of excellent fluorescent characteristics of quantum dots and by means of combining quantum dot fluorescence labeling technology and immunochromatographic assay; detecting fluorescence signal strength of a quantitative belt and a quality control belt by the aid of a fluorescence quantometer and correcting the fluorescence strength of the quantitative belt by the aid of the quality control belt after immunochromatographic assay of the test strip; and further quantitatively detecting analyte according to a standard curve obtained by the fluorescence quantometer. The method is simple, rapid, accurate, low in cost and quite high in sensitivity. Compared with a conventional colloidal gold immunochromatographic assay method, the method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The method is applicable to samples such as blood samples, urine samples, spittle, excrement and the like, and can be applied to detection of critical illness, poison, food safety and the like.

Description

technical field [0001] The present invention relates to a method for quantitative detection of analytes by using fluorescent quantum dot labeling ligands based on optimal materials, and particularly discloses a high-sensitivity quantum dot fluorescence immunochromatography method, which can realize the detection of pathogens, major diseases (such as tumors) , cardiovascular disease), illegal drugs, drug testing, food safety and other analytes semi-quantitative and quantitative detection, which belongs to the field of fluorescence immunoassay technology. Background technique [0002] Immunochromatography (immunochromatography) is a rapid diagnostic technology that has emerged in the past ten years. Taking the double-antibody sandwich method as an example, the principle is to immobilize specific antibodies on a certain area of ​​the chromatographic membrane (such as nitrocellulose membrane) first. When the sample (urine or serum) is dropped on one end of the dry chromatographi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558
Inventor 王东
Owner BEIJING KANGMEI TIANHONG BIOTECH
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