Fluorescence immunochromatographic assay and kit for quantitative detection of troponin I/creatine kinase isoenzyme/myohemoglobin

Abstract

The invention discloses a quantum dot multicolor marking method for quantitative detection of various cardiovascular disease markers and a kit of troponin I / creatine kinase isoenzyme / myohemoglobin. The method realizes fluorescent quantitative detection by utilizing excellent fluorescent properties of quantum dots and combining a multicolour marking technology and an immunochromatographic assay on the basis of optimizing each component of a test strip. Compared with the common collaurum immunochromatographic assay, the method has the advantages of good mark stability, low nonspecificity, high sensitivity, wide linear range, small cross interference, and accuracy in quantification. The kit disclosed by the invention is used for carrying out quantification detection on the troponin I, the creatine kinase isoenzyme and the myohemoglobin simultaneously, is suitable for detection of whole blood, blood serum and plasma samples, can provide a reference for cardiovascular and cerebrovascular disease diagnosis, and is widely applied to primary hospitals and clinics.

Description

technical field [0001] The invention relates to a method for quantitatively detecting biochemical markers using quantum dot-labeled antibodies based on the multi-color labeling properties of quantum dots, and particularly discloses a troponin I / creatine kinase isoenzyme / myosin The protein quantitative detection kit can simultaneously realize the quantitative detection of troponin I, creatine kinase isozyme and myoglobin, and belongs to the field of medical testing. Background technique [0002] Cardiovascular disease is a great threat to human health and life, and has become one of the two major difficulties in medicine. Cardiovascular disease is the "number one killer" of human death in the world. There are nearly 200 million patients with cardiovascular disease in my country. Nearly 3 million people died of cardiovascular disease. Therefore, it is imminent to establish a detection system that can accurately, quickly and conveniently predict and diagnose cancer and cardiova...

AI Technical Summary

Problems solved by technology

[0016] The technical problem to be solved in the present invention is to provide a fluorescent immunochromatographic method for detection in view of the shortcomings of low sensitivity and mutual interference between markers in the detection of multiple biochemical markers in the prior art

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