System for quantitatively detecting heavy metal cadmium and preparation method thereof
A quantitative detection and heavy metal technology, applied in the field of heavy metal immunodiagnosis system, can solve the problems of high instrument and detection cost, long reaction time, unsatisfactory, etc., and achieve the effect of reducing non-specific adsorption, simple and fast operation, and low cost
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[0040] Example 1 The quantitative detection system for heavy metal cadmium of the present invention and its preparation
[0041] Such as Figure 1-3 As shown, the system for quantitative detection of heavy metal cadmium of the present invention includes a buckle 12, a fluorescence immunochromatography test strip 9 and a dilution buffer, where the buckle 12 is the outer shell structure of the fluorescence immunochromatography test strip, including sample loading Hole 10 and observation window 11.
[0042] The structure of the fluorescence immunochromatographic test strip is as figure 1 with figure 2 As shown, the sample pad 1, the marking pad 2, the chromatographic membrane 6, the absorbent pad 7 and the bottom plate 8 are included. When testing samples with a lot of particles, the test strip also includes a particle separation membrane 3. Among them, the sample pad 1, the marking pad 2, the particle separation membrane 3, the chromatography membrane 6 and the absorbent pad 7 are...
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[0052] Example 2: Quantitative detection of heavy metal cadmium by the quantitative detection system of the present invention for heavy metal cadmium
[0053] 1. Coupling of fluorescent dye and antibody
[0054] Mix the fluorescent dye rhodamine with emission wavelength of 665nm and 1 mg / ml heavy metal cadmium (Cd) monoclonal antibody, react at room temperature for 3 hours, add 1mol / L hydroxylamine hydrochloride to stop the reaction, and separate and purify by chromatography column or chromatography column to obtain Fluorescent dye modified heavy metal cadmium (Cd) monoclonal antibody. In the same way, rabbit IgG (quality control molecule) modified with the fluorescent dye rhodamine was obtained. The fluorescence emission wavelength of the fluorescent dye of the heavy metal cadmium (Cd) antibody and the fluorescence emission wavelength of the fluorescent dye of the modified rabbit IgG are both 665 nm.
[0055] 2. System construction
[0056] a) Preparation of bonding pad
[0057] Mix...
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