System for quantitatively detecting heavy metal cadmium and preparation method thereof

Abstract

The invention provides a system for quantitatively detecting heavy metal cadmium, which comprises a buckle, a fluorescence immunochromatography test strip and a sample dilution buffer solution, wherein the buckle is of an outer shell structure of the fluorescence immunochromatography test strip and is provided with a sample adding hole and an observation window; the fluorescence immunochromatography test strip comprises a sample pad, a marking pad, a chromatography membrane, a water absorption pad and a bottom plate; a fluorescent dye modified heavy metal cadmium specific antibody and a fluorescent dye modified quality control molecule are fixed on the marking pad at the same time; a heavy metal cadmium chelating agent hapten is fixed on the quantitative detection line; the hapten is specifically combined with a fluorescent dye modified heavy metal cadmium specific antibody fixed on the marking pad; biomolecules capable of being specifically combined with the quality control moleculesare fixed on the quality control line; and a to-be-detected sample is treated with the sample dilution buffer solution before detection. In order to detect the heavy metal cadmium in the sample, treatment by using the sample dilution buffer solution is carried out before the to-be-detected sample is detected, and the sample dilution comprises a chelating agent which is combined with the heavy metal cadmium to form a heavy metal cadmium chelating agent hapten; if the sample dilution buffer solution is not used for treating a sample, the sample is directly detected, the heavy metal cadmium in the sample cannot be detected, and the existence of the sample dilution buffer solution is crucial to the detection of the cadmium in the sample.

Description

technical field [0001] The invention relates to the field of heavy metal immunodiagnosis systems in the fields of medical testing, environmental testing and food safety, in particular to a system for quantitatively detecting heavy metal cadmium and a preparation method thereof. Background technique [0002] In recent decades, with the rapid development of the global economy, the rapid development of the metal industry, such as metal smelting and electroplating, is accompanied by the industrial wastewater discharged when creating economic value. In the pollution caused by the wastewater of these industries, heavy metals Pollution accounts for a large part of it. The original meaning of heavy metals is metals with a density greater than 4.59 per cubic centimeter. Among the atomic numbers 1-90, there are 54 metal elements that are heavy metals in terms of density, but they are really heavy metals in industry. Only 10 metal elements such as nickel, diamond, copper, mercury, tin,...

AI Technical Summary

Problems solved by technology

The above methods have high analytical sensitivity, but the operation is complicated, the reaction time is long, and the cost of instruments and detection is high.

Unable to meet the needs of grassroots hospitals, grassroots environmental monitoring agencies and food testing agencies

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