123 results about "N Acetyl D Glucosamine" patented technology

C-glycoside compounds are suited for stimulating the synthesis of glycosaminoglycans containing a D-glucosamine and / or N-acetyl-D-glucosamine residue, advantageously hyaluronic acid, and / or proteoglycans, advantageously proteoglycans containing hyaluronic acid, by fibroblasts and / or keratinocytes.

The invention provides compositions and methods useful for the treatment and / or prevention of interstitial cystitis and / or a related urinary tract condition in man or in animals. Specifically, provided are compositions specially formulated for direct instillation into the bladder and / or parenteral use in the treatment and / or prevention of interstitial cystitis. Compositions adapted for direct instillation into the bladder and / or for systemic administration are provided comprised of therapeutic amounts of: chondroitin sulfate in combination with hyaluronan (hyaluronic acid) are provided. Compositions adapted for direct instillation into the bladder and / or for systemic administration are also provided comprised of therapeutic amounts of: chondroitin sulfate, hyaluronan (hyaluronic acid) and N-acetyl D-glucosamine.

The present invention provides a composition, and a method of use thereof for treating connective tissue damage in man and in animals, which comprises a therapeutically effective amount of chondroitin sulfate, N-acetyl D-glucosamine, and hyaluronan (hyaluronic acid). Particularly, the present invention provides a composition, and a method of use thereof, for treating connective tissue damage including, but not limited to, arthritic disease, osteoarthritis, rheumatoid arthritis, osterochondrosis dessicans, cartilage damage, joint injury, joint inflammation, joint synovitis, degenerative joint disease (DJD), post surgical DJD, traumatic injury, fracture, tendon damage, ligament damage, skeletal damage, musculoskeletal damage, fiber damage, adipose tissue damage, blood cell damage, and plasma damage. Compositions for delivery of the present invention include those for parenteral, oral, and transmucosal delivery and for direct surgical placement onto the affected tissues.

The invention discloses a method for preparing N-acetyl-D-glucosamine from chitin. The method includes 7 steps of dissolution, filtration, degradation, crystallization, filtration, washing, and drying. The method for preparing N-acetyl-D-glucosamine from chitin uses recyclable solvent and solid acid, adapts to the current requirements of environmental protection, basically reaches clean production with no pollution; and the raw material or product in the whole reaction process can be dissolved, thus avoiding the formation of by-products, and greatly improving the the yield of N-acetyl-D-glucosamine product up to 89%. The preparation process is simple, and does not require special procedures; therefore, the invention has broad application prospects, and can bring great economic benefits and social benefits.

The present invention relates to novel C-glycoside compounds of given absolute configuration, to a process for synthesising them and to compositions containing them. The invention also relates to the cosmetic use of these C-glycoside compounds as agents to stimulate the synthesis of glycosaminoglycans containing a D-glucosamine and / or N-acetyl-D-glucosamine residue, advantageously hyaluronic acid, and / or of proteoglycans, advantageously proteoglycans containing hyaluronic acid, by fibroblasts and / or keratinocytes. The invention also relates to a cosmetic process for treating keratin materials using a composition containing at least one C-glycoside compound according to the invention.

The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes β-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.

The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes β-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.

Glycans are identified which have high affinity for C. difficile toxin A. They share one of two saccharide backbones and may have additional side chains. The backbones are galactose-β1-3 N-acetyl-D-glucosamine-β-1-3-galactose-β-1-4-N-acetyl-D-glucosamine and galactose-α-1-3-galactose-β-1-4-N-acetyl-D-glucosamine. The ligands may be used therapeutically, prophylactically, and diagnostically.

N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB 10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

The present invention provides compositions containing a mixture of cholesterol sulfate and an exfoliant. The exfoliant can be N-acetyl-D-glucosamine, N-acetylgalactosamine, or a combination thereof. The combination of cholesterol sulfate with the exfoliant surprisingly enhances the skin barrier even though the activity of each of the components is opposite the other. In addition, because of the ability to enhance or repair the skin barrier function, methods of maintaining or improving a healthy skin barrier are included in the present invention by apply to the skin an effective amount of the mixture of cholesterol sulfate with the exfoliant. The mixture can also be useful in treating or preventing damage to the skin, where the damage is caused by a comprised skin barrier function. As a result of improved skin barrier function, the appearance of lines and wrinkles is generally reduced; rough and dry skin conditions are also improved.

The invention provides a biodegradable hyaluronic acid derivative including at least one modified hyaluronic acid repeating unit represented by the formula (HA)-[O(C═O)NH-M]p, wherein HA is a unit including N-acetyl-D-glucosamine and D-glucuronic acid, M is a modifying moiety containing a C2-16 hydrocarbyl group, and p is an integer of 1 to 4.

C-glycoside compounds are suited for stimulating the synthesis of glycosaminoglycans containing a D-glucosamine and / or N-acetyl-D-glucosamine residue, advantageously hyaluronic acid, and / or proteoglycans, advantageously proteoglycans containing hyaluronic acid, by fibroblasts and / or keratinocytes.

The invention relates to a non-genetic recombinant strain for producing N-acetyl-D-glucosamine and D-glucosamine through microorganism fermentation, wherein the strain has been preserved in China General Microbiological Culture Collection Center. A bacillus subtilis strain NJ090259 is assigned the accession number of CGMCC10257. A bacillus licheniformis strain NJ091195 is assigned the accession number of CGMCC10258. The two strains are both preserved at 29th, December, 2014. The invention provides the novel strain for producing N-acetyl-D-glucosamine and D-glucosamine and the production method thereof. By means of the method, stable production and supplement of the N-acetyl-D-glucosamine can be achieved. The invention also achieves non-animal-sourced and safe production of the N-acetyl-D-glucosamine and the D-glucosamine. The method is short in production period, is low in cost and is more environment-friendly.

A biodegradable hyaluronic acid derivative comprising a modified hyaluronic acid repeating unit represented by the formula (HA)-[O(C═O)NH-M]p, wherein HA is a unit including N-acetyl-D-glucosamine and D-glucuronic acid, M is a modifying moiety containing a C2-16 hydrocarbyl group or a prepolymer, and p is an integer of 1 to 4. The biodegradable hyaluronic acid derivative when dissolved in a hydrophilic medium can form micelles and can be used to entrap a pharmaceutically active or bioactive molecule.

This Invention relates to, for production of N-acetyl-D-glucosamine and D-glucosamine by microbial fermentation: A nongenetic recombinant strain, preserved in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms; NJ090259, a strain of Bacillus subtilis, Preservation No. CGMCC10257; and NJ091195, a strain of Bacillus licheniformis, Preservation No. CGMCC10258 and Preservation Date Dec. 29, 2014. The beneficial effects of this Invention are: Provide a new strain for production of N-acetyl-D-glucosamine and D-glucosamine, and its production methods. By this method, it may achieve stable production and supply of N-acetyl-D-glucosamine, and may also achieve non-animal-derived, safety production of N-acetyl-D-glucosamine and D-glucosamine; the method is of short production phase and low cost, and is more environmentally-friendly.

The invention discloses a method for separating and extracting N-acetyl-D-glucosamine and D-glucosamine from ammonia sugar fermentation liquor. The method includes the steps of microfiltration, ultrafiltration, nanofiltration and the like, the fermentation liquor is filtered through a microfiltration membrane with the aperture of 0.1-10 micrometers to remove impurity particles and microbial thalli, protein, nucleic acid, colloidal particle large molecules and other impurities are removed through filtering by means of an ultrafiltration membrane with the aperture of 0.001-0.1 micrometer, and pigment, polypeptide, nucleotide and other small-molecular impurities are removed through a reverse osmosis system; N-acetyl-D-glucosamine is crystallized and separated from the fermentation filtrate containing N-acetyl-D-glucosamine and glucosamine. The method includes the steps of fermentation filtrate heating and concentration, seed crystal addition, temperature-control crystallization, N-acetyl-D-glucosamine crystal collection, residual glucosamine recycling and the like. The method is low in cost and does not influence the structure of N-acetyl-D-glucosamine, and the obtained N-acetyl-D-glucosamine is high in purity.

The invention discloses a culture-medium of pig semen. Per 100ml of the culture-medium contains the following components: 5-20 g of lactose, 0.005-0.05 g of N-acetyl-D-glucosamine, 0.05-0.5 g of DHA, 1.0-5.0 ml of glycerine, 0.2-1.5 ml of OEP, 10-40 ml of yolk and 50,000-200,000 IU of penicillin and / or streptomycin. The invention also discloses a method for processing the pig semen. Proved by detection, the activity of the pig semen processed by the method reaches 0.3-0.6, and the acrosome integrity exceeds 50 percent, thereby completely conforming to the requirement of artificial fertilization at present.

The present invention has an object of revealing an epidermis regeneration effect of various chitin derivatives and providing an agent for therapy or treatment for wound which can be clinically used effectively. According to the present invention, it has been evidenced that N-acetyl-D-glucosamine has an effect of promoting proliferation of an epidermis keratinized cell. Accordingly, the present invention provides a proliferation promoting agent for epidermis keratinized cell comprising N-acetyl-D-glucosamine. According to another aspect of the present invention, there is provided a regeneration promoting agent or protecting agent for epithelium, skin or dermis comprising the proliferation promoting agent. Further, a therapeutic agent for wound is provided.

The present invention provides a composition, and a method of use thereof for treating connective tissue damage in man and in animals, which comprises a therapeutically effective amount of chondroitin sulfate, N-acetyl D-glucosamine, and hyaluronan (hyaluronic acid). Particularly, the present invention provides a composition, and a method of use thereof, for treating connective tissue damage including, but not limited to, arthritic disease, osteoarthritis, rheumatoid arthritis, osterochondrosis dessicans, cartilage damage, joint injury, joint inflammation, joint synovitis, degenerative joint disease (DJD), post surgical DJD, traumatic injury, fracture, tendon damage, ligament damage, skeletal damage, musculoskeletal damage, fiber damage, adipose tissue damage, blood cell damage, and plasma damage. Compositions for delivery of the present invention include those for parenteral, oral, and transmucosal delivery and for direct surgical placement onto the affected tissues.

A semen diluent of blue for is prepared from N-acetyl-D-glucosamine, fructose, sodium citrate trihydroxylmethyl amonomethane, buffer, bacteriacide and distilled water. The diluent pH value is 6.20-6.80 and its osmotic pressure is 300-350 mOsmol / L.

The invention provides a method for removing acetyl and coupling, adsorbing and separating D-glucosamine hydrochloride. The method comprises the following steps: carrying out pretreatment on a solution containing N-acetyl-D-glucosamine, and enabling the solution to pass through an acetyl removal and adsorption and separation device filled with cation exchange resin; removing the acetyl of the N-acetyl-D-glucosamine under a certain condition, and adsorbing and separating the D-glucosamine hydrochloride. According to the method provided by the invention, an advanced process of removing the acetyl of the N-acetyl-D-glucosamine and adsorbing and separating D-glucosamine to replace a traditional process of removing the acetyl by hydrochloric acid hydrolysis and concentrating and crystallizing to separate the D-glucosamine hydrochloride. The D-glucosamine hydrochloride produced by the method has the advantages of good product quality, high extraction yield, low consumption of auxiliary materials, simplicity and convenience for operation and small environment pollution.

The invention provides a preparation method of high-purity N-acetyl-D-glucosamine, and relates to monosaccharide. The invention provides the preparation method of the high-purity N-acetyl-D-glucosamine with the advantages that the operation is simple, the production cost is low, and economy and environment-friendly effects are achieved. The method comprises the following steps of (1) chitin is used as a raw material, and a degradation agent of sulphuric acid is used for performing degradation reaction on the chitin; (2) filtering is performed to remove unreacted chitin, and unreacted sulphuric acid is removed by a membrane method; (3) calcium hydroxide solid is added into a solution subjected to unreacted sulphuric acid removal by the membrane method, and the pH value is regulated to be 5.5 to 7.0; (4) separated precipitates are removed through filtering, and the filtered solution is decolored by activated carbon; (5) absolute ethyl alcohol is added into the decolored solution until no precipitates separate out; (6) the precipitates are filtered and collected; the precipitates are dissolved by water; then, the absolute ethyl alcohol is added for separating out solid; next, the solid is collected and dried, and the product of N-acetyl-D-glucosamine powder is obtained. The product purity can be greater than 98 percent.

The invention discloses a method for preparing high-purity N-acetyl-D glucosamine. The method comprises the following steps: preparing a solution from N-acetyl-D glucosamine, performing ultrafiltration with a ceramic membrane so as to obtain filtrate, putting a proper amount of activated carbon into the filtrate, filtering by using a sealed filtering machine so as to obtain a decolored liquid, performing electrodialysis desalting on the decolored liquid with an electrodialysis membrane so as to obtain a desalted liquid of which the conductivity is 5-50mu s / cm; performing evaporation concentration on the desalted liquid by using an evaporator so as to obtain a high-purity concentrated liquid; putting the high-purity concentrated liquid into a crystal cylinder, adding an organic solvent, andseparating a crystal so as to obtain N-acetyl-D glucosamine crystal slurry; pouring the crystal slurry into a centrifugal machine, performing solid-liquid separation so as to obtain a high-purity N-acetyl-D glucosamine wet crystal, and drying the wet crystal in a vacuum drying machine, so as to obtain the high-purity N-acetyl-D glucosamine of which the content is greater than or equal to 99.50%.Compared with the prior art, the method is low in cost, high in yield, short in process cycle and high in product purity.

A chitosan solution is formed from chitin, which is a homopolymer of beta (1-4)-linked N-acetyl-D-glucosamine. Rinsed, dried and ground chitin undergoes a process of deacetylation to convert some N-acetyl glucosamine to glucosamine, a primary component of chitosan. The chitosan solution is prepared by mixing 5 chitosan with an alpha-hydroxy acid such as glycolic acid. An aqueous chelated silver solution is prepared by mixing silver oxide with a carboxylic acid such as citric acid. The chitosan solution can then be mixed with the silver solution resulting in a cationic complex. The cationic complex of the present invention may then be electrostatically bonded with generally negatively charged surfaces. In use, citrate promotes uptake of the silver by microbes. The antimicrobial complex can be applied via several methods of application, including an impregnated wipe, a foam, a gel, a spray, a lotion and an ointment.

The invention discloses a method for producing glucosamine. The provided method for producing glucosamine comprises the following steps: N-acetyl-D-glucosamine as a raw material is used for producingglucosamine under the action of an engineering bacterium; the engineering bacterium is a recombinant bacterium expressing functional protein and is obtained by introducing a functional gene encoding the functional protein into a starting bacterium; the functional protein is N-acetyl-6-glucosamine phosphate deacetylase or chitobiose deacetylase. The method for preparing glucosamine has the advantages of mild reaction conditions, simple process route and high product purity, and is suitable for large-scale industrial production, and very suitable for popularization and application.