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Culture-medium of pig semen and method for processing pig semen

A technology of pig semen and cryopreservation liquid, which is applied in the biological field and can solve the problems of no pig semen diluent additive, sperm density freezing and thawing method, etc.

Inactive Publication Date: 2009-12-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when using the large tube method, there is no ideal diluent additive, sperm density, freezing and thawing methods for porcine semen, etc.

Method used

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  • Culture-medium of pig semen and method for processing pig semen
  • Culture-medium of pig semen and method for processing pig semen
  • Culture-medium of pig semen and method for processing pig semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0084] After the above-mentioned general operation steps 1 to 4, a mixed solution containing pig semen with the use concentration of each component as shown in Table 2 is obtained; then, the large tube method is frozen according to step 5.

[0085] Table 2

[0086]

[0087]

Embodiment 5-9

[0095] After the above-mentioned general operation steps 1 to 4, a mixed solution containing pig semen with the use concentration of each component as shown in Table 4 is obtained; then, the large tube method is frozen according to step 5.

[0096] Table 4

[0097]

[0098]

Embodiment 10-14

[0105] After the above-mentioned general operation steps 1 to 4, a mixed solution containing pig semen with the use concentration of each component as shown in Table 6 is obtained; then, the large tube method is frozen according to step 5.

[0106] Table 6

[0107]

[0108]

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PUM

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Abstract

The invention discloses a culture-medium of pig semen. Per 100ml of the culture-medium contains the following components: 5-20 g of lactose, 0.005-0.05 g of N-acetyl-D-glucosamine, 0.05-0.5 g of DHA, 1.0-5.0 ml of glycerine, 0.2-1.5 ml of OEP, 10-40 ml of yolk and 50,000-200,000 IU of penicillin and / or streptomycin. The invention also discloses a method for processing the pig semen. Proved by detection, the activity of the pig semen processed by the method reaches 0.3-0.6, and the acrosome integrity exceeds 50 percent, thereby completely conforming to the requirement of artificial fertilization at present.

Description

Technical field [0001] The invention relates to biotechnology, in particular to a method for processing pig semen cryopreservation liquid and pig semen. Background technique [0002] Semen freezing technology has extremely important application value for giving full play to the genetic potential of excellent breeding pigs and the preservation of genetic resources of local pig breeds. The research of pig semen freezing technology began in 1956, Polge et al. reported the first time that pig frozen semen was used to obtain litters. However, for decades, compared with cattle, sheep and other livestock, the application of frozen semen in pigs has been quite limited, partly because of the low vitality of pig frozen semen, the low conception rate, and the relatively small number of litters; part of the reason is because of pigs. The volume of semen is large, and the traditional straw (0.25 / 0.5ml) is inconvenient to operate in practical application; while the freezing of the pellet metho...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张德福戴建军殷方芝吴彩凤吴华莉刘东
Owner SHANGHAI ACAD OF AGRI SCI
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