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Diluent for sperm of blue fox

A technique of diluting liquid and semen, applied in the field of animal reproduction, can solve problems such as no reports, and achieve the effects of easy collection, improved survival index, and easy promotion.

Inactive Publication Date: 2005-01-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also reported that N-acetyl-D-glucosamine has been successfully applied in porcine semen frozen dilution (Yi et al., 2002, Animal Reproduction Science.74, 187-194.), but about N-acetyl-D-glucose The study of amines on blue fox semen dilution has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 (diluent 1)

[0037] First weigh 0.07g of N-acetyl-D-glucosamine, dissolve it in 5ml of distilled water, and dissolve it completely. Weigh 7.15g of fructose and dissolve it in 20ml of distilled water. After it is completely dissolved, add 1.68g of tris, 1.25g of sodium citrate, and 2.25g of disodium hydrogen phosphate, and add them one by one in the above order. After the added reagent is completely dissolved, add the latter one. After dissolving, mix the two preparations, then add 100,000 units of penicillin, and finally distilled water to a constant volume of 100ml. After constant volume, the pH value is 6.25, and the osmotic pressure is 340mOsmo / L. Store in a 5°C refrigerator. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on cryopreservation of blue fox semen at ultra-low temperature. When used, it is mixed with the blue fox semen at a ratio of 1:2, and at the same time, the temperature of the...

Embodiment 2

[0039] Embodiment 2: (diluent 2)

[0040]Weigh 0.08g of N-acetyl-D-glucosamine, dissolve it in 5ml of distilled water, and store it in a refrigerator at 5°C after it is completely dissolved. Weigh 6.83g of fructose and dissolve it in 20ml of distilled water. After it is completely dissolved, add 1.05g of tris, 0.78g of sodium citrate, and 1.86g of potassium dihydrogen phosphate, and add them one by one in the above order. After the added reagent is completely dissolved, add the latter one, mix well after dissolving, and store in a refrigerator at 5°C. When in use, take out and mix the two preparations, then add 100,000 units of penicillin, and finally distilled water to make up to 100ml, after the constant volume, the pH value is 6.24, and the osmotic pressure is 315mOsmo / L. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on cryopreservation of blue fox semen at ultra-low temperature. When used, it is mixed with the b...

Embodiment 3

[0041] Embodiment 3: (diluent 3)

[0042] First weigh 7.46g of fructose and dissolve it in 20ml of distilled water. After it is completely dissolved, add 1.48g of trishydroxymethylaminomethane, 0.61g of sodium citrate, and 1.75g ​​of sodium dihydrogen phosphate, and add them one by one in the above order. After the reagent added first is completely dissolved, add the latter one. After dissolving, mix well and store in a refrigerator at 5°C. When in use, take it out and adjust it to the temperature of the semen to be preserved or the temperature of artificial insemination, then weigh 0.05g of N-acetyl-D-glucosamine, and dissolve it in the adjusted diluent. Finally, 100,000 units of penicillin was added, and finally the volume of distilled water was 100 ml. After the volume was constant, the pH value was 6.25, and the osmotic pressure was 305 mOsmo / L. All preparation processes are carried out under sterile conditions. This formula has an excellent effect on the dilution and pr...

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PUM

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Abstract

A semen diluent of blue for is prepared from N-acetyl-D-glucosamine, fructose, sodium citrate trihydroxylmethyl amonomethane, buffer, bacteriacide and distilled water. The diluent pH value is 6.20-6.80 and its osmotic pressure is 300-350 mOsmol / L.

Description

technical field [0001] The invention relates to a diluent of blue fox semen, which is used for artificial insemination of raising blue foxes and belongs to the technical field of animal reproduction. Background technique [0002] The blue fox (Alopex lagopus), also known as the arctic fox, is a precious fur animal, and its skin is a popular fur raw material. At present, the artificial breeding of blue foxes has been widely carried out in North China and Northeast China. There are nearly 1.5 million breeding foxes in stock. It has become a good project for local farmers to get rid of poverty and become rich. However, during the long-term breeding of blue foxes, due to the limitations of feed nutrition, disease prevention and other conditions, especially the proliferation of inbreeding, it directly led to the degradation of artificially raised blue fox species in my country, the quality of the skins was poor, and the breeding return rate was low. The international fur market h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李新红华修国
Owner SHANGHAI JIAO TONG UNIV
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