Method for producing glucosamine

A technology of glucosamine and glucose, which is applied in the field of genetic engineering, can solve the problems of being unsuitable for people with seafood allergies, easily causing allergic reactions, and serious environmental pollution, etc., and achieves the effects of being suitable for popularization and application, high product purity, and simple process route

Active Publication Date: 2018-05-25
JIANGSU HEVI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chitin hydrolysis method is the production of glucosamine from chitin in the shells of shrimp and crabs. This method has caused overfishing of marine resources, and the waste liquid produced by the processing process has serious environmental pollution. The resulting product is prone to allergic reactions and is not suitable for people with seafood allergies

Method used

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  • Method for producing glucosamine
  • Method for producing glucosamine
  • Method for producing glucosamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, construct recombinant bacteria

[0073] 1. Construction of recombinant bacteria Ⅰ

[0074] The recombinant plasmid pBAD / HisB-EcDac was constructed. According to the sequencing results, the structure of the recombinant plasmid pBAD / HisB-EcDac is described as follows: the small fragment between the XhoI and EcoRI restriction sites of the vector pBAD / HisB is replaced with the double-stranded DNA molecule shown in Sequence 1 of the Sequence Listing. The DNA molecule shown in Sequence 1 of the Sequence Listing encodes the protein shown in Sequence 2 of the Sequence Listing. The protein shown in sequence 2 of the sequence listing is N-acetyl-6-phosphate glucosamine deacetylase derived from Escherichia coli (Escherichia coli), referred to as EcDac protein.

[0075] The recombinant plasmid pBAD / HisB-EcDac was introduced into Escherichia coli BW25113 to obtain recombinant bacterium I.

[0076] 2. Construction of recombinant bacteria II

[0077] The recombinant ...

Embodiment 2

[0087] Embodiment 2, application of recombinant bacteria to prepare glucosamine

[0088] 1. Preparation of bacteria

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Abstract

The invention discloses a method for producing glucosamine. The provided method for producing glucosamine comprises the following steps: N-acetyl-D-glucosamine as a raw material is used for producingglucosamine under the action of an engineering bacterium; the engineering bacterium is a recombinant bacterium expressing functional protein and is obtained by introducing a functional gene encoding the functional protein into a starting bacterium; the functional protein is N-acetyl-6-glucosamine phosphate deacetylase or chitobiose deacetylase. The method for preparing glucosamine has the advantages of mild reaction conditions, simple process route and high product purity, and is suitable for large-scale industrial production, and very suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for producing glucosamine. Background technique [0002] Glucosamine, also known as Glucosamine (GlcN), referred to as ammonia sugar, widely exists in animal exoskeletons and microbial cell walls. Glucosamine is widely used in food, health care, cosmetics, medicine and other industries. Glucosamine is an important nutrient for the formation of cartilage tissue cells. It is a natural component of healthy articular cartilage tissue. It can help repair and maintain cartilage tissue and stimulate the growth and development of cartilage tissue cells. [0003] At present, the production method of glucosamine is mainly chitin hydrolysis. The chitin hydrolysis method is the production of glucosamine from chitin in the shells of shrimps and crabs. This method has caused overfishing of marine resources, and the waste liquid produced by the processing and treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12N1/21C12R1/19
CPCC12N9/80C12P19/26C12Y305/01033
Inventor 胡美荣王雷陶勇
Owner JIANGSU HEVI BIOTECH CO LTD
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