Method for preparing N-acetyl-D-glucosamine from chitin

A technology of glucosamine and chitin, applied in the field of chemical degradation, can solve the problems of increasing industrial cost, complicated process, complicated operation, etc., and achieve the effects of large economic and social benefits, simple preparation process and broad application prospect.

Active Publication Date: 2016-10-12
JIANGSU AOXIN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of N-acetyl-D-glucosamine by microbial fermentation requires the cultivation of microbial strains, followed by fermentation, separation and other processes. The whole process is complicated, the operation is cumbersome, and the yield is very low, which is not conducive to industrial production. Chemical production of N -Acetyl-D-glucosamine, the preparation process requires a lot of toxic and harmful chemical reagents, not only there are chemical residues in the product N-acetyl-D-glucosamine, but also chemical pollution is not conducive to environmental protection, and the whole process is extremely prone to combustion, explosion, etc. Accident, enzymatic hydrolysis to prepare N-acetyl-D-glucosamine, no matter what kind of enzyme is needed, it needs to increase the industrial cost, and the yield is also very low, which is not conducive to industrial production. Three kinds of N-acetyl-D-glucosamine preparation methods, all contain expensive and difficult steps for the preparation of N-acetyl-D-glucosamine, and these methods all have the problem of very low yields

Method used

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  • Method for preparing N-acetyl-D-glucosamine from chitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] like figure 1 Shown, a kind of method that chitin prepares N-acetyl-D-glucosamine comprises the following steps:

[0028] 1) Dissolving: dissolving chitin and solvent in an ultrasonic stirring tank to obtain a crude mother liquor, the solvent being a fluorinated reagent;

[0029] 2) Filtration: The crude mother liquor prepared in step 1) is continuously pumped into a microporous membrane filter by a diaphragm pump to filter to obtain a fine mother liquor;

[0030] 3) Degradation: Place the refined mother liquor and solid acid prepared in step 2) in a degradation tank for degradation to obtain a degradation liquid, and the solid acid is SO 2- 4 / MxOy type superacid;

[0031] 4) Crystallization: pump the degradation solution prepared in step 3) into the tank and ultrasonically stir the tank and add ethanol to complete the precipitation of N-acetyl-D-glucosamine;

[0032] 5) Filtration: filter the solution after crystallization in step 4) to obtain crude N-acetyl-D-glu...

Embodiment 2

[0037] like figure 1 Shown, a kind of method that chitin prepares N-acetyl-D-glucosamine comprises the following steps:

[0038] 1) Dissolving: placing chitin and a solvent in an ultrasonic stirring tank for dissolving to obtain a crude mother liquor, the solvent being an amide / lithium chloride system;

[0039] 2) Filtration: The crude mother liquor prepared in step 1) is continuously pumped into a microporous membrane filter by a diaphragm pump to filter to obtain a fine mother liquor;

[0040] 3) Degradation: Place the refined mother liquor and solid acid prepared in step 2) in a degradation tank for degradation to obtain a degradation liquid, and the solid acid is SO 2- 4 / MxOy type superacid;

[0041] 4) Crystallization: pump the degradation solution prepared in step 3) into the tank and ultrasonically stir the tank and add ethanol to complete the precipitation of N-acetyl-D-glucosamine;

[0042] 5) Filtration: filter the solution after crystallization in step 4) to ob...

Embodiment 3

[0047] like figure 1 Shown, a kind of method that chitin prepares N-acetyl-D-glucosamine comprises the following steps:

[0048] 1) Dissolving: placing chitin and a solvent in an ultrasonic stirring tank for dissolving to obtain a crude mother liquor, the solvent being an amino acid salt;

[0049] 2) Filtration: The crude mother liquor prepared in step 1) is continuously pumped into a microporous membrane filter by a diaphragm pump to filter to obtain a fine mother liquor;

[0050] 3) Degradation: placing the refined mother liquor and solid acid prepared in step 2) in a degradation tank for degradation to obtain a degradation liquid, and the solid acid is a strongly acidic cation exchange resin;

[0051] 4) Crystallization: pump the degradation solution prepared in step 3) into the tank and ultrasonically stir the tank and add ethanol to complete the precipitation of N-acetyl-D-glucosamine;

[0052] 5) Filtration: filter the solution after crystallization in step 4) to obtai...

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Abstract

The invention discloses a method for preparing N-acetyl-D-glucosamine from chitin. The method includes 7 steps of dissolution, filtration, degradation, crystallization, filtration, washing, and drying. The method for preparing N-acetyl-D-glucosamine from chitin uses recyclable solvent and solid acid, adapts to the current requirements of environmental protection, basically reaches clean production with no pollution; and the raw material or product in the whole reaction process can be dissolved, thus avoiding the formation of by-products, and greatly improving the the yield of N-acetyl-D-glucosamine product up to 89%. The preparation process is simple, and does not require special procedures; therefore, the invention has broad application prospects, and can bring great economic benefits and social benefits.

Description

technical field [0001] The invention relates to the technical field of chemical degradation, in particular to a method for preparing N-acetyl-D-glucosamine from chitin. Background technique [0002] Amino sugars are generally used as monosaccharide residues in complex oligosaccharides and polysaccharides, glucosamine is an amino derivative of monosaccharide glucose, and N-acetyl-D-glucosamine is an acetylated derivative of glucosamine. Glucosamine, N-acetyl-D-glucosamine and other amino sugars are important building blocks of many natural polysaccharides. The existing preparation methods of N-acetyl-D-glucosamine mainly include the preparation of N-acetyl-D-glucosamine by microbial fermentation, the preparation of N-acetyl-D-glucosamine by chemical method, and the preparation of N-acetyl-D-glucosamine by enzymatic hydrolysis. D-glucosamine. The preparation of N-acetyl-D-glucosamine by microbial fermentation requires the cultivation of microbial strains, followed by ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/06C07H1/00C07H1/06
CPCC07H1/00C07H1/06C07H13/06
Inventor 王松叶詹金明陈延静陈进利赵艳凯
Owner JIANGSU AOXIN BIOTECHNOLOGY CO LTD
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