215results about How to "Reduce detection steps" patented technology

The invention provides a kit suitable for rapidly detecting AMH and INHB by using a double-tagging time resolution fluorescence immunoassay method. The kit comprises (1) a solid phase carrier coated by an AMH coating antibody and an INHB coating antibody; (2) a mixed calibration product of AMH and INHB; (3) an AMH detection antibody marked by using a lanthanide element 1; (4) an INHB detection antibody marked by a lanthanide element 2; (5) an immunoreaction accelerant liquid; (6) a concentrated washing liquid; and (7) a reinforcing liquid. The invention further provides a use method of the kit for rapidly detecting AMH and INHB by using the double-tagging time resolution fluorescence immunoassay method. By adopting a double-tagging time resolution fluorescence immunoassay technique, the defect that conventional AMH and INHB respectively need a single kit, that is, one kit can be only used for detecting AMH or INHB can be overcome, the detection steps can be reduced, the sampling time can be shortened, and the use amount of a blood sample can be reduced.

The invention discloses an electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor comprises a work electrode, a counter electrode, a reference electrode and a electrolytic cell; the work electrode is a polymer fixed microbial electrode, and the polymer fixed microbial electrode comprises an electrode and a polymer and bacteria mixed layer coated outside the electrode; and the material of the above polymer is gelatin or chitosan, the above bacteria comprise Escherichia coli and / or yeast, and the electrode is a glassy carbon electrode, a gold electrode, a platinum electrode or a boron-doped diamond film electrode. The invention also discloses an apparatus of the electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor can instantly monitor the water biotoxicity change and detect the water biotoxicity value, can realize instant, online and continuous detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.

The integral detection and measurement method of metering performance of high voltage power meter belongs to the field of measurement technology. The method includes connecting high voltage voltage / current generator unit, voltage / current regulating unit, data comparator unit, control and data processing unit, and standard high voltage metering unit with the high voltage power meter to be measured; applying one sequence of voltage and current to the standard high voltage metering unit and the high voltage power meter to be measured to obtain one set of standard power values and one set of measured power values; comparing these two sets of power values in the data comparison unit and feeding the comparison results to the control and data processing unit; calculating the metering error based on the given formula and outputting the measurement results.

The invention provides a method for detecting Vibrio parahaemolyticus. The sequence of the forward primer is SEQ ID NO:1, the sequence of the reverse primer is SEQ ID NO:2, and the sequence of the probe is SEQ ID NO:3. A real-time RPA (recombinase polymerase amplification) process is utilized to detect the food-borne pathogen Vibrio parahaemolyticus, thereby implementing safe, specific, quick, sensitive and simple field quick detection on the Vibrio parahaemolyticus, and further overcoming the defects in the existing traditional detection technique. The method is suitable for field detection, can effectively inhibit the epidemic situation caused by Vibrio parahaemolyticus food poisoning in time, and perfects the food safety control system.

The embodiment of the application discloses a beam-based channel detection method and equipment, which are used to improve the beam-based channel detection efficiency and improve the use efficiency ofunlicensed spectra. The method disclosed by the embodiment of the application includes the following steps: when the equipment needs to occupy a channel where a spectrum is located to perform data transmission, the equipment determines a main beam and performs a complete channel detection process on the main beam, and when determining that the channel where the the spectrum is located in the direction of the main beam can be occupied, the equipment adopts a simplified channel detection process with fewer detection steps relative to the complete channel detection process for channel detectionin the directions of other beams. Thereby, the overall channel detection steps can be reduced, and the channel detection efficiency can be improved.

The invention discloses a method for cultivating a breeding material with broad spectrum and lasting spike blast resistance, belonging to the technical field of rice molecular breeding. The method is characterized in that with a parent of conventional rice or hybrid rice with large production extension area and excellent comprehensive characters as a recurrent parent, broad-spectrum anti-disease genes, Pigm and Pi54, are polymerized in the breeding material through continuous backcross and convergent cross and with combination of marker-assisted selection; meanwhile, representative strains are selected for performing artificial inoculation identification of spike blast, natural inducedidentification in rice blast serious areas and basic agronomic character evaluation during a total growth period, and an advanced line with excellent spike blast resistance is obtained. The agronomic characters of the advanced line bred through the method are similar to or consistent with those of the recurrent parent, and the broad spectrum and lasting resistance to spike blast is enhanced obviously.

The invention relates to the field of mechanical part machining and particularly relates to a shaft part defective product detection and repair equipment and method. The shaft part defective product detection and repair equipment comprises a base and a conveying belt, wherein the conveying belt is arranged at the top of the base. The shaft part defective product detection and repair equipment further comprises a controller, a machining table, a screening mechanism and a repair mechanism, wherein the machining table is arranged at the top of the base and used for carrying a shaft part, the screening mechanism is arranged at the top of the base and used for detecting the shaft part, the screening mechanism comprises a material taking assembly and an image recognition assembly, the repair mechanism is arranged at the top of the base and used for repairing the shaft part, the repairing mechanism comprises a stand column, a spraying assembly and a polishing assembly, and the conveying belt,the material taking assembly, the image recognition assembly, the spraying assembly and the polishing assembly are electrically connected with the controller. According to the shaft part defective product detection and repair equipment and method, shaft parts with different damages can be repaired, meanwhile the damages can be removed perfectly, and the repair quality is high; and besides, the detection efficiency is high, the repair process can be easily shortened, and the overall repair efficiency can be improved.

The invention provides a moving target detection method and device, electronic equipment and a storage medium, and relates to the technical field of image processing. The method comprises the following steps: obtaining a first frame image and a second frame image which are adjacent and a rotation matrix and a translation matrix between the first frame image and the second frame image, the first frame image and the second frame image comprising the same moving target; Extracting a plurality of first feature points from the first frame image; Determining a plurality of second feature points corresponding to the plurality of first feature points from the second frame image according to the second frame image and the plurality of first feature points; Calculating a plurality of distances between the plurality of second feature points and a plurality of corresponding polar lines according to the rotation matrix and the translation matrix, wherein the plurality of corresponding polar lines are a plurality of polar lines of the plurality of first feature points on the second frame image; Screening out a plurality of third feature points on the moving target according to the plurality of distances; And detecting and obtaining a moving target according to the plurality of third feature points. According to the invention, the data calculation amount and the moving target detection stepsare reduced.

The invention discloses a method for quick and nondestructive detection of dry matter content in tea based on 11 characteristic wavelengths. The method comprises the following steps of: acquiring diffuse reflection spectrum reflectivities of a tea sample at the 11 characteristic wavelength positions, wherein the 11 characteristic wavelengths comprise 404 nm, 409 nm, 421 nm, 461 nm, 676 nm, 695 nm, 710 nm, 733 nm, 755 nm, 972 nm and 1036 nm; and converting the diffuse reflection spectrum reflectivities to absorbances so as to obtain absorbance values of the tea sample at the 11 characteristic wavelength positions, and then calculating to obtain the dry matter content in the tea sample. By adopting the method disclosed by the invention, the dynamic variation of the dry matter content in thetea processing process can be quickly and effectively monitored, thus quick, nondestructive and low-cost detection of dry matters in the tea processing process is realized.

The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting lead ions and a manufacturing method of the kit. An elisa plate enveloped by lead ion envelope antigens, lead-ion monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a lead ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The indirect competition enzyme linked immunoreagent kit can detect the lead irons in a trace mode, is used for detecting the lead iron contamination residual in the environment, the soil, water, foods and containers, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit not only can be used for screening samples in large batch, but also can perform rapid detection on samples in small batch, and provides effective means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.

The invention relates to the technical field of biological medicines, in particular to a kit for quantum dot nucleic acid detection of urinary tract infection-causing pathogens. The kit comprises a detection membrane strip, a fluorescent detection solution and reaction solutions, wherein the detection membrane strip comprises a nylon membrane and a capture probe fixed to the nylon membrane; the fluorescent detection solution comprises quantum dots for marking the capture probe and coupled with streptavidin on the surfaces; the reaction solutions include a reaction solution I, a reaction solution II, a reaction solution III and a reaction solution IV. The kit has the beneficial effects as follows: the high-throughput, high-sensitivity and high-specificity kit for the quantum dot nucleic acid detection of the urinary tract infection-causing pathogens is provided; the kit has fewer steps and obviously shorter detection time than the existing colorimetric gene chip, has lower equipment cost than an organic fluorescent gene chip, and is conducive to clinical popularization.

The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting mercury ions and a manufacturing method of the kit. An elisa plate enveloped by mercury ion envelope antigens, mercury-ion-resisting monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a mercury ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The kit can detect the mercury irons in a trace mode, is used for detecting the mercury irons in the environment, the soil, water, foods, medicines, cosmetics and the like, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit is low in requirement for pretreating samples, simple in treatment process, not only can be used for screening the samples in large batch, but also can perform rapid detection on the samples in small batch, and not only provides technical support for environment and food safety, but also provides effective technological means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.

The invention provides a method for detecting the concentration of a mixed acid solution. In the prior art, the concentrations of various acids in a mixed acid to be detected cannot be accurately measured, or a detection process is complicated, the consumed time is long and more materials are used. The method for detecting the concentration of the mixed acid solution comprises the following steps: providing the mixed acid to be detected with a pre-set volume and a pH compound electrode; titrating with alkali liquid and detecting to obtain a first equivalent point and a second equivalent point by the pH compound electrode; determining the concentration of total hydrogen ions and the concentration of fluosilicic acid in the mixed acid through the first equivalent point and the second equivalent point; carrying out acid titration to recover the pH value of the mixed acid to be neutral; then adding lanthanum nitrate to titrate and detecting by the pH compound electrode to obtain a third equivalent point; and determining the concentrations of hydrofluoric acid and nitric acid through the third equivalent point, the concentration of the fluosilicic acid and the concentration of the total hydrogen ions. By adopting the method, the concentrations of all the acids in the mixed acid of the three acids can be accurately detected, the complexity of detection and calculation is extremely reduced and the professional requirements on detectors are lowered.

The invention relates to a 500kV line relay protection device detection method and system. The method comprises the following steps: receiving a test order of to-be-detected relay protection devices and a test parameter and a standard fixed value of each preset logic protection test case, which are input by a user; acquiring sampling precision of the current to-be-detected relay protection device; generating a test sequence according to the logic protection test case and the test parameter corresponding to the current to-be-detected relay protection device when the sampling precision is greater than a preset precision threshold value; outputting the test sequence to the to-be-detected relay protection device, receiving an action message returned by the to-be-detected relay protection device, and generating detection result information in a preset format of the current to-be-detected relay protection device; generating a detection result of a next relay protection device according to the test order until the detection results of all the to-be-detected relay protection devices are generated. According to the method and the system, the detection accuracy can be effectively improved, the detection steps of testers can be saved, and the time required for detecting 500kV line relay protection devices is shortened.

The invention relates to an indirect competitive enzyme linked immunosorbent assay (elisa) kit for detecting chromium ions as well as preparation and detection methods thereof. A trivalent chromic ion envelope antigen-enveloped elisa plate, a trivalent chromic ion-resistant monoclonal antibody, a second enzyme-labeled antibody, a substrate color-developing solution, a stop solution, a trivalent chromic ion standard solution, a lotion concentrated solution and a sample processing solution are arranged in the kit. Samples are processed before detection so that Cr3<+> in the samples forms Cr<3+>-EDTA (ethylene diamine tetraacetic acid), and then the chromium ions are detected by the kit. The chromium ion enzyme linked immunosorbent assay kit can be used for detecting the trace amount of chromium ions, has the Cr<3+> detection sensitivity of 2ng / ml, has the characteristics of quickness, simplicity and convenience, sensitivity, specificity, economy and the like, has few detection steps, saves the detection time, and reduces the operation error; the detection cost is less than 1 / 20 of that of a physical and chemical analysis method, the timeliness is high, the field detection can be performed, and the kit is mainly used for screening large-batch samples of chromium ion pollution residues in environments, soil, water and foods.

The invention discloses a blood viscosity fast detection device and a method thereof. The upper end of a sample feeding needle is connected with one end of an electromagnetic valve, the other end of the electromagnetic valve is connected with a peristaltic pump through a detection pipe, a baroceptor is arranged on the detection pipe between the electromagnetic valve and the peristaltic pump, and the pipe diameter of the detection pipe is greater than the pipe diameter of the sample feeding needle. The method adopts a negative pressure method for detecting the viscosity of blood samples, and collects the pressure change data in the detection pipe according to a principle that the flowing speed of fluids with different viscosities is in inverse proportion to the viscosities, and the viscosities of the fluids can be measured through the calculation via a pressure-viscosity mathematical model. The invention has the characteristics of high measurement precision, ingenious design, simple structure, easy implementation, good reliability and the like.

The invention discloses a leakage detector, belonging to the technical field of natural gas engine. The leakage detector comprises an encloser, wherein the encloser is internally provided with a water tank; a lifting device for the placement of a to-be-detected fuel gas part is arranged above the water tank; the encloser is also internally provided with a gas source for providing compressed gas to the to-be-detected fuel gas part; the encloser is provided with an opening allowing the fuel gas part to come in and go out; a safety door is arranged at the opening; and the encloser is also provided with a view window for viewing the fuel gas part in the water tank. After such the structure is adopted, the to-be-detected fuel gas part is connected with the gas source, the fuel gas part is placed on the lifting device, and the lifting device is responsible for placing the fuel gas part into the water in the water tank, so that the gas source supplies the gas to the to-be-detected fuel gas part to detect the gas tightness of the fuel gas part under the precondition of closing the safety door, and a detecting person can obtain the gas tightness condition of the fuel gas part safely and directly outside the encloser through a view window.

The invention discloses a cable tensile strength detection device. The cable tensile strength detection device comprises a guide rod and a tensile test group which is slidably connected with the guiderod and is used for fixing a test cable; one side of the tensile test group is provided with a control device for driving the tensile test group to carry out tensile test on the test cable; the tensile test group comprises two tensile test blocks; at least one auxiliary test block is arranged between the two tensile test blocks; the two tensile test blocks are respectively used for clamping two ends of the test cable; when in use, a plurality of connecting pieces can be synchronously contracted through the driving device; and the distance between the tensile test blocks and the auxiliary testblock can be synchronously increased or decreased, so that the tensile amount change data between all sections of the test cable can be visually reflected through the offset of the auxiliary test block when the test cable is stretched; and the detection steps of the cable can be effectively reduced during detection.

The invention relates to a preparation method of a renewable electrochemical immunosensor capable of being used for detecting a biomarker for forecasting and diagnosing an acute coronary syndrome, i.e., a soluble CD40 ligand (sCD40L), and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following step: immobilizing an sCD40L antibody through a carboxyl functionalized multi-wall carbon nano tube-polyethyleneimine-gold nanoparticle nano compound (c-MWCNTs-PEI-AuNPs) serving as a substrate material to realize capturing of sCD40L and further quantitatively detecting the sCD40L. A c-MWCNTs-PEI-AuNps nano compound is easy to prepare, high in conductivity, relatively high in stability and relatively large in specific surface area, so that a large quantity of antibodies can be firmly immobilized; furthermore, by the use of specificity identification of the antibodies and antigens, the built electrochemical immunosensor is relatively high in specificity. The electrochemical immunosensor has the advantages of high sensitivity, high specificity, convenience, quickness and capability of being repetitively used for multiple times; a new method is supplied to detection of the sCD40L, and available information is supplied to clinical forecasting and diagnosis of the acute coronary syndrome.

The invention provides a nucleic acid qualitative detection method based on nano-particles and rolling circle amplification. The detection method mainly comprises the steps of: allowing silica nanoparticles in a disperse state in a solution before the rolling circle amplification is used; performing the rolling circle amplification when a target nucleic acid molecule is present; and gathering the silica nanoparticles to form a macroscopic white blocky substance in the solution so as to achieve the purpose of qualitatively detecting the target nucleic acid molecule. As the presence of the target nucleic acid molecule can be judged by gathering states of the silica nanoparticles before and after rolling circle amplification, a large number of trace nucleic acid molecules are amplified, results are rapidly and intuitively detected without a special detection instrument, the detection cost is reduced, and the nucleic acid qualitative detection method is suitable for popularization of clinical detection.

The invention relates to a method for installing and detecting a lifting flipper guide rail. The method for installing and detecting the lifting flipper guide rail comprises the following steps that the data of a trunk are checked, whether the precision and the length after the trunk is mounted in sections are within the error requirement range or not is checked, the guide rail is welded to a guide rail connecting plate together, the guide rail is hung into the trunk, the guide rail is arranged on a supporting plate, an adjusting bolt is arranged on a bracket temporarily welded on the inner wall of the trunk, the position of the guide rail is adjusted through the adjusting bolt, the sizes of L, L1, L2, H, H1 and H2 are measured, and compared with design values, if the size exceeds 2mm, theguide rail is adjusted up and down until the error range is within 2mm; a tool is disassembled, the guide rail is fixed by the adjusting bolt, spot welding is carried out on the guide rail connectingplate and the supporting plate, and then the guide rail connecting plate and the supporting plate are welded; and the tool is used for recheck to ensure the normal installation of the guide rail. Byusing the method for installing and detecting the lifting flipper guide rail to measure a bow and a stern of a measuring tool, the installation precision and quality of the guide rail are improved, and the production efficiency in the shipbuilding process is improved.

The invention relates to a magnetic immunity detection method for antigens (antibodies), which comprises the steps: (1) respectively coupling the antibodies (antigens) to the surfaces of magnetic polymer microspheres and a micro-pore plate; (2) instilling the detected antigens into the micro-pore plate; (3) cleaning the micro-pore plate and then adding the magnetic polymer microspheres in the micro-pore plate; and (4) cleaning the micro-pore plate once again and stretching the magnetic head of a magnetoresistive sensor into the pore of the micro-pore plate, and determining the number of the magnetic polymer microspheres on the surface of the micro-pore plate according to the variation between former output voltage and latter output voltage of the magnetic reluctance sensor, thus further determining the presence and the concentration of the virus antigens (antibodies). The method has the characteristics of few detection steps, high speed, high sensitivity and accuracy, low cost and the like.

The invention provides an optical fiber cantilever beam sensor for food pathogenic bacteria and a detection method. A single-mode optical fiber is etched by using ultra-short laser pulse and a cantilever beam with a rectangular shape is finally formed on one end face of the single-mode optical fiber; a Fabry-Perot cavity is formed by the cantilever beam and the end face of the single-mode optical fiber; after a process of plating a gold film on the surface, pathogenic antigens to be detected are uniformly adsorbed on the surface of the cantilever beam; the optical fiber cantilever beam sensor is completely immersed into a solution containing pathogenic antigens; the cantilever beam is deformed and bent due to the specific bonding of the antibodies and the antigens; the size of the deformation amount reflects concentration information of the pathogenic bacteria in a solution to be detected; a signal demodulating system takes a common incandescent light bulb as a light source to generate broadband spectral information; the standard Fabry-Perot cavity is formed by one face of the cantilever beam and the end face of the single-mode optical fiber; and lengths of two faces in phase information can be demodulated. The optical fiber cantilever beam sensor has the advantages of high sensitivity, high detection speed and small needed detection samples.

The invention discloses a relay fault detection method for a grid-connected inverter. The method comprises the steps of: enabling resistors to be connected in parallel between inverter relays Relay1 and Relay2 and grid-side inverters Relay3 and Relay4, and carrying out the sampling of two sides of each resistor to obtain a Relay sampling signal; performing driving to disconnect all the relays, anddetermining whether the relay Relay3 on the power grid side has an adhesion fault or not or whether the relay Relay3 and the relay Relay4 simultaneously have an adhesion fault or not; driving the relay Relay3 on the power grid side to be closed; determining whether the relay Relay4 on the power grid side has an adhesion fault or not, outputting an open-loop voltage on the inverter side, performing driving to disconnect the relay Relay3 on the power grid side, and determining whether the relay Relay1 on the inverter side has an adhesion fault or whether the relay Relay1 and the relay Relay2 have an adhesion fault or not; outputting an open-loop voltage on the inverter side to drive the relay Relay1 and the relay Relay2 on the inverter side to be closed, and determining whether the relay Relay1 and / or the relay Relay2 of the inverter cannot be closed or not; and closing the open-loop voltage of the inverter side, driving the relay Relay1 and the relay Relay2 on the inverter side to be disconnected, closing the relay Relay3 and the relay Relay4 on the power grid side, and determining whether the relay Relay3 and / or the relay Relay4 on the power grid side cannot be closed or not. Thedetection steps are reduced.

The invention relates to a preparation method of an electrochemical DNA biosensor for detection of LPL gene SNP (rs1801177), and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the steps: firstly, the surface of a nitrogen-doped graphene (N-G) sheet is loaded with a large number of palladium-platinum bimetal (PdPt) nanoparticles, then polyaniline is formed on the surface of the nitrogen-doped graphene (N-G) sheet by in-situ polymerization to obtain a nano composite material, next, a single-stranded DNA signal probe is mixed with the composite material, and a redox probe is prepared; secondly, a capture probe is immobilized on the surface of a sensor by a C60 / PAMAM / Au nanocomposite material, and the electrochemical DNA biosensor for quantitative detection of rs1801177 is prepared. The biosensor is successfully applied to detection of single-base mutation of a lipoprotein lipase gene. The biosensor has the advantages of high sensitivity, strong specificity, and convenient and fast detection, provides a new method for detection of rs1801177, and provides a theoretical basis and an experimental basis for clinical diagnosis and prevention of cardiovascular diseases.

The invention discloses an enzyme linked immunosorbent assay kit for detecting sparfloxacin, which comprises a kit body, wherein the kit body is internally provided with a sparfloxacin specific antibody, a conjugate and an enzyme labeled second antibody of the sparfloxacin and carrier protein, a sparfloxacin antigen or specific antibody coated plate, a sparfloxacin standard solution, a substrate development solution, a cleaning solution and a stop solution, and the sparfloxacin specific antibody is a sparfloxacin monoclonal antibody. Main reagents in the enzyme linked immunosorbent assay kit of the sparfloxacin are provided in a working solution form, and the enzyme linked immunosorbent assay kit is convenient to use and low in price, has low requirements on pretreatment of a sample during detection, is simple in treatment process, can be used for simultaneously and quickly screening massive samples, and has the characteristics of quickness, simplicity, convenience, accuracy, high sensitivity and the like in comparison to an instrumental analysis technique.

The invention provides a method and a system for detecting cell killing efficacy and application of the method and the system. The method for detecting cell killing efficacy comprises the following steps: microscopic images of a co-culture sample on a cell counting slide are acquired, the co-culture sample is a cell sample obtained by co-culturing a target cell and an effector cell, the target cell carries a first fluorescent label, the co-culture sample at least uses a second fluorescent label for dyeing labeling, the microscopic images include a bright field microscopic image, a first fluorescent microscopic image and at least one second fluorescent microscopic image, and then the bright field microscopic image, the first fluorescent microscopic image and the at least one second fluorescent microscopic image are subjected to to superposing synthetic analysis and counted, and a detection result of the cell killing efficacy is obtained. According to the detection method, the image information and the data processing result of the to-be-detected cell can be simultaneously obtained, the obtained result is more direct, few detection steps are needed, and the detection efficiency is high.

Trap chain covalent united to PCR tube wall is used in directional trap of destination segment in nucleic acid coarse body fluid, and the destination segment is hybrid combined onto the inner wall of PCR tube. After the impurity is washed out, the trapped nucleic acid may be hybrid detected directly with marker probe or the trapped nucleic acid may be used as template for PCR in the tube and the solid phase PCR product is hybrid detected or for real-time fluorescent PCR in the tube.

The invention discloses a fatigue driving detection method, which comprises the following steps: acquiring a head image by a near-infrared 3D camera; two consecutive head images are tracked by LK optical flow method, and mouth feature points, head feature points and eye feature points are extracted from the tracked head images; searching an eye image according to the position coordinates of the eye feature points to extract the eye feature points of the eye image; according to the eye feature points, mouth feature points and head feature points of the eye image within the predetermined acquisition times, the eyelid movement features, mouth movement features and head movement features being determined one-to-one, and fed into the SVM classifier, so that the SVM classifier judges the fatiguedriving state and realizes fatigue driving detection. The invention also provides a fatigue driving detection device. By adopting the fatigue driving detection method and the fatigue driving detection device of the invention, the accuracy and the judgment speed of the fatigue driving state judgment can be effectively improved.