214results about How to "Reduce detection steps" patented technology

The invention provides a kit suitable for rapidly detecting AMH and INHB by using a double-tagging time resolution fluorescence immunoassay method. The kit comprises (1) a solid phase carrier coated by an AMH coating antibody and an INHB coating antibody; (2) a mixed calibration product of AMH and INHB; (3) an AMH detection antibody marked by using a lanthanide element 1; (4) an INHB detection antibody marked by a lanthanide element 2; (5) an immunoreaction accelerant liquid; (6) a concentrated washing liquid; and (7) a reinforcing liquid. The invention further provides a use method of the kit for rapidly detecting AMH and INHB by using the double-tagging time resolution fluorescence immunoassay method. By adopting a double-tagging time resolution fluorescence immunoassay technique, the defect that conventional AMH and INHB respectively need a single kit, that is, one kit can be only used for detecting AMH or INHB can be overcome, the detection steps can be reduced, the sampling time can be shortened, and the use amount of a blood sample can be reduced.

The embodiment of the application discloses a beam-based channel detection method and equipment, which are used to improve the beam-based channel detection efficiency and improve the use efficiency ofunlicensed spectra. The method disclosed by the embodiment of the application includes the following steps: when the equipment needs to occupy a channel where a spectrum is located to perform data transmission, the equipment determines a main beam and performs a complete channel detection process on the main beam, and when determining that the channel where the the spectrum is located in the direction of the main beam can be occupied, the equipment adopts a simplified channel detection process with fewer detection steps relative to the complete channel detection process for channel detectionin the directions of other beams. Thereby, the overall channel detection steps can be reduced, and the channel detection efficiency can be improved.

The invention discloses a method for cultivating a breeding material with broad spectrum and lasting spike blast resistance, belonging to the technical field of rice molecular breeding. The method is characterized in that with a parent of conventional rice or hybrid rice with large production extension area and excellent comprehensive characters as a recurrent parent, broad-spectrum anti-disease genes, Pigm and Pi54, are polymerized in the breeding material through continuous backcross and convergent cross and with combination of marker-assisted selection; meanwhile, representative strains are selected for performing artificial inoculation identification of spike blast, natural inducedidentification in rice blast serious areas and basic agronomic character evaluation during a total growth period, and an advanced line with excellent spike blast resistance is obtained. The agronomic characters of the advanced line bred through the method are similar to or consistent with those of the recurrent parent, and the broad spectrum and lasting resistance to spike blast is enhanced obviously.

The invention relates to the technical field of biological medicines, in particular to a kit for quantum dot nucleic acid detection of urinary tract infection-causing pathogens. The kit comprises a detection membrane strip, a fluorescent detection solution and reaction solutions, wherein the detection membrane strip comprises a nylon membrane and a capture probe fixed to the nylon membrane; the fluorescent detection solution comprises quantum dots for marking the capture probe and coupled with streptavidin on the surfaces; the reaction solutions include a reaction solution I, a reaction solution II, a reaction solution III and a reaction solution IV. The kit has the beneficial effects as follows: the high-throughput, high-sensitivity and high-specificity kit for the quantum dot nucleic acid detection of the urinary tract infection-causing pathogens is provided; the kit has fewer steps and obviously shorter detection time than the existing colorimetric gene chip, has lower equipment cost than an organic fluorescent gene chip, and is conducive to clinical popularization.

The invention provides a method for detecting the concentration of a mixed acid solution. In the prior art, the concentrations of various acids in a mixed acid to be detected cannot be accurately measured, or a detection process is complicated, the consumed time is long and more materials are used. The method for detecting the concentration of the mixed acid solution comprises the following steps: providing the mixed acid to be detected with a pre-set volume and a pH compound electrode; titrating with alkali liquid and detecting to obtain a first equivalent point and a second equivalent point by the pH compound electrode; determining the concentration of total hydrogen ions and the concentration of fluosilicic acid in the mixed acid through the first equivalent point and the second equivalent point; carrying out acid titration to recover the pH value of the mixed acid to be neutral; then adding lanthanum nitrate to titrate and detecting by the pH compound electrode to obtain a third equivalent point; and determining the concentrations of hydrofluoric acid and nitric acid through the third equivalent point, the concentration of the fluosilicic acid and the concentration of the total hydrogen ions. By adopting the method, the concentrations of all the acids in the mixed acid of the three acids can be accurately detected, the complexity of detection and calculation is extremely reduced and the professional requirements on detectors are lowered.

The invention relates to a 500kV line relay protection device detection method and system. The method comprises the following steps: receiving a test order of to-be-detected relay protection devices and a test parameter and a standard fixed value of each preset logic protection test case, which are input by a user; acquiring sampling precision of the current to-be-detected relay protection device; generating a test sequence according to the logic protection test case and the test parameter corresponding to the current to-be-detected relay protection device when the sampling precision is greater than a preset precision threshold value; outputting the test sequence to the to-be-detected relay protection device, receiving an action message returned by the to-be-detected relay protection device, and generating detection result information in a preset format of the current to-be-detected relay protection device; generating a detection result of a next relay protection device according to the test order until the detection results of all the to-be-detected relay protection devices are generated. According to the method and the system, the detection accuracy can be effectively improved, the detection steps of testers can be saved, and the time required for detecting 500kV line relay protection devices is shortened.

The invention discloses a leakage detector, belonging to the technical field of natural gas engine. The leakage detector comprises an encloser, wherein the encloser is internally provided with a water tank; a lifting device for the placement of a to-be-detected fuel gas part is arranged above the water tank; the encloser is also internally provided with a gas source for providing compressed gas to the to-be-detected fuel gas part; the encloser is provided with an opening allowing the fuel gas part to come in and go out; a safety door is arranged at the opening; and the encloser is also provided with a view window for viewing the fuel gas part in the water tank. After such the structure is adopted, the to-be-detected fuel gas part is connected with the gas source, the fuel gas part is placed on the lifting device, and the lifting device is responsible for placing the fuel gas part into the water in the water tank, so that the gas source supplies the gas to the to-be-detected fuel gas part to detect the gas tightness of the fuel gas part under the precondition of closing the safety door, and a detecting person can obtain the gas tightness condition of the fuel gas part safely and directly outside the encloser through a view window.

The invention discloses a cable tensile strength detection device. The cable tensile strength detection device comprises a guide rod and a tensile test group which is slidably connected with the guiderod and is used for fixing a test cable; one side of the tensile test group is provided with a control device for driving the tensile test group to carry out tensile test on the test cable; the tensile test group comprises two tensile test blocks; at least one auxiliary test block is arranged between the two tensile test blocks; the two tensile test blocks are respectively used for clamping two ends of the test cable; when in use, a plurality of connecting pieces can be synchronously contracted through the driving device; and the distance between the tensile test blocks and the auxiliary testblock can be synchronously increased or decreased, so that the tensile amount change data between all sections of the test cable can be visually reflected through the offset of the auxiliary test block when the test cable is stretched; and the detection steps of the cable can be effectively reduced during detection.

The invention provides a nucleic acid qualitative detection method based on nano-particles and rolling circle amplification. The detection method mainly comprises the steps of: allowing silica nanoparticles in a disperse state in a solution before the rolling circle amplification is used; performing the rolling circle amplification when a target nucleic acid molecule is present; and gathering the silica nanoparticles to form a macroscopic white blocky substance in the solution so as to achieve the purpose of qualitatively detecting the target nucleic acid molecule. As the presence of the target nucleic acid molecule can be judged by gathering states of the silica nanoparticles before and after rolling circle amplification, a large number of trace nucleic acid molecules are amplified, results are rapidly and intuitively detected without a special detection instrument, the detection cost is reduced, and the nucleic acid qualitative detection method is suitable for popularization of clinical detection.

The invention provides an optical fiber cantilever beam sensor for food pathogenic bacteria and a detection method. A single-mode optical fiber is etched by using ultra-short laser pulse and a cantilever beam with a rectangular shape is finally formed on one end face of the single-mode optical fiber; a Fabry-Perot cavity is formed by the cantilever beam and the end face of the single-mode optical fiber; after a process of plating a gold film on the surface, pathogenic antigens to be detected are uniformly adsorbed on the surface of the cantilever beam; the optical fiber cantilever beam sensor is completely immersed into a solution containing pathogenic antigens; the cantilever beam is deformed and bent due to the specific bonding of the antibodies and the antigens; the size of the deformation amount reflects concentration information of the pathogenic bacteria in a solution to be detected; a signal demodulating system takes a common incandescent light bulb as a light source to generate broadband spectral information; the standard Fabry-Perot cavity is formed by one face of the cantilever beam and the end face of the single-mode optical fiber; and lengths of two faces in phase information can be demodulated. The optical fiber cantilever beam sensor has the advantages of high sensitivity, high detection speed and small needed detection samples.

The invention discloses an enzyme linked immunosorbent assay kit for detecting sparfloxacin, which comprises a kit body, wherein the kit body is internally provided with a sparfloxacin specific antibody, a conjugate and an enzyme labeled second antibody of the sparfloxacin and carrier protein, a sparfloxacin antigen or specific antibody coated plate, a sparfloxacin standard solution, a substrate development solution, a cleaning solution and a stop solution, and the sparfloxacin specific antibody is a sparfloxacin monoclonal antibody. Main reagents in the enzyme linked immunosorbent assay kit of the sparfloxacin are provided in a working solution form, and the enzyme linked immunosorbent assay kit is convenient to use and low in price, has low requirements on pretreatment of a sample during detection, is simple in treatment process, can be used for simultaneously and quickly screening massive samples, and has the characteristics of quickness, simplicity, convenience, accuracy, high sensitivity and the like in comparison to an instrumental analysis technique.

The invention discloses a nucleic acid aptamer fluorescence sensor for detecting myoglobin and a construction method of the nucleic acid aptamer fluorescence sensor and belongs to the technical fieldof analysis and detection. The construction method includes the following steps that: a myoglobin nucleic acid aptamer modified with a quencher and a nucleic acid aptamer complementary strand modifiedwith a fluorophore are added into a buffer system so as to be hybridized, and therefore, a nucleic acid aptamer fluorescence sensor can be prepared; when myoglobin is added, the nucleic acid aptameris separated from the complementary short strand, and is combined with the myoglobin; the complementary short strand connected with the fluorophore is separated from the nucleic acid aptamer connectedwhich the quencher, so that the fluorescence of the fluorophore is recovered; and the intensity of the recovery of the fluorescence is proportional to the concentration of the myoglobin, and therefore, the concentration of the myoglobin can be measured. According to the sensor of the invention, the nucleic acid aptamer fluorescence sensor which is inexpensive and easy to prepare is adopted to detect the myoglobin, and therefore, the myoglobin can be accurately detected. The method has the advantages of simple design, low cost, few detection steps, simple operation, high sensitivity and good selectivity.