Indirect competitive enzyme linked immunosorbent assay kit for detecting chromium ions as well as preparation and detection methods thereof

Abstract

The invention relates to an indirect competitive enzyme linked immunosorbent assay (elisa) kit for detecting chromium ions as well as preparation and detection methods thereof. A trivalent chromic ion envelope antigen-enveloped elisa plate, a trivalent chromic ion-resistant monoclonal antibody, a second enzyme-labeled antibody, a substrate color-developing solution, a stop solution, a trivalent chromic ion standard solution, a lotion concentrated solution and a sample processing solution are arranged in the kit. Samples are processed before detection so that Cr3<+> in the samples forms Cr<3+>-EDTA (ethylene diamine tetraacetic acid), and then the chromium ions are detected by the kit. The chromium ion enzyme linked immunosorbent assay kit can be used for detecting the trace amount of chromium ions, has the Cr<3+> detection sensitivity of 2ng / ml, has the characteristics of quickness, simplicity and convenience, sensitivity, specificity, economy and the like, has few detection steps, saves the detection time, and reduces the operation error; the detection cost is less than 1 / 20 of that of a physical and chemical analysis method, the timeliness is high, the field detection can be performed, and the kit is mainly used for screening large-batch samples of chromium ion pollution residues in environments, soil, water and foods.

Description

technical field [0001] The invention relates to the fields of ELISA and chromium ion detection, in particular to an ELISA kit for detecting chromium ions and its assembly and detection method. Background technique [0002] Chromium is an element that widely exists in nature, distributed in soil, air, water and organisms, mainly in the form of trivalent chromium (Cr 3+ ) and hexavalent chromium (Cr 6+ ) exists in the form of Chromium is one of the essential trace elements for humans and animals, Cr 3+ Has the activity of glucose tolerance factor (GTF), participates in the regulation of cholesterol and glucose metabolism in organisms, Cr 3+ Lack of it will cause fat deposition on the inner wall of blood vessels in the body and lead to arteriosclerosis. But Cr 3+ and Cr 6+ Another toxic substance, Cr 6+ The toxicity is Cr 3+ 100 times more toxic, many studies have confirmed that Cr 3+ Carcinogenic and mutagenic; Cr 6+ Carcinogenic toxicity, mucous membrane toxicity, l...

AI Technical Summary

Problems solved by technology

In August 2011, the Qujing chromium slag pollution incident, the hexavalent chromium content detected in the groundwater outlet exceeded the standard by more than 200 times; in April 2012, the chromium-excessive medicinal capsule incident caused economic losses of more than 16 billion yuan

[0004] At present, the established food chromium detection techniques mainly include graphite furnace atomic absorption spectrometry (GFAAS), flame atomic absorption spectrometry (FAAS), inductively coupled plasma mass spectrometry (ICP-MS), and inductively coupled plasma atomic emission spectrometry. (ICP-AES), hydride generation-atomic fluorescence spectrometry (HG-AFS), electrochemical analysis methods, etc., but these physical and chemical analysis methods require expensive equipment and skilled professionals, and the detection is cumbersome, time-consuming, and the cycle is long. High, cannot be operated on site, and the amount of sample screening is small, etc., its application is limited

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