Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof

A water biological and electrochemical technology, applied in the direction of measuring devices, electrochemical variables of materials, scientific instruments, etc., can solve the problems of water body control that cannot be suspected of toxicity, biological toxicity detection lag, and the impact of turbidity detection, etc., to achieve easy instrumentation and On-site testing, shortening the testing steps, improving convenience and speed

Active Publication Date: 2014-01-15
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the most widely used microbial experiment is the luminescent bacteria method as a national standard method to detect biological toxicity. The toxicity of the water body is reflected by detecting the inhibitory effect of toxic substances on the luminous intensity of luminescent bacteria. This method is relatively mature, and there are also a series of related methods on the market The instrument comes out; but its detection is easily affected by the turbidity of the water itself, and the cost of the instrument is high
In addition, the degree of pollution can be judged by microbial biochemical oxygen demand; however, biological oxygen demand usually requires water samples to be filled in a completely closed dissolved oxygen bottle, and cultured in a dark place at 20°C for 5 days, which will cause biological toxicity. The detection lags behind, and people cannot control the suspected toxic water in time

Method used

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  • Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof
  • Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof
  • Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] An electrochemical sensor for instant monitoring and detection of aquatic biotoxicity:

[0072] 1. Preparation of polymer-immobilized microbial electrodes

[0073] Commercially available glassy carbon (glassy carbon, GC) was polished with 1000 mesh metallographic sandpaper for 10 minutes, and then washed with 0.3 μM, 0.15 μM, 0.01 μm α-Al 2 o 3 Polish to a mirror surface, then use 10ml of deionized water (the water used in each embodiment of the present invention is prepared by a Millipore Milli-Q pure water meter), ethanol, and acetone for 10 minutes, and place in the air to dry , and polished glassy carbon flakes (0.7cm×0.7cm) were obtained as the flat electrode substrate.

[0074] Escherichia coli culture solution is in 50ml deionized water, add 0.25g sodium chloride, 0.15g yeast extract and 0.5g peptone. After adjusting the concentration of the culture solution to pH=7.0 with 0.1M sodium hydroxide, it was sterilized in an autoclave.

[0075] Use disodium hydroge...

Embodiment 2

[0091] The operation method and steps are the same as those in Example 1 to evaluate the biological toxicity of heavy metal ions, the only change is: add Hg to the system 2+ to Hg 2+ The concentration in the system is 80μg / ml, and the Hg of 80μg / ml is detected 2+ The degree of biological toxicity, by calculation, Hg 2+ The inhibition rate was 71.9%,

Embodiment 3

[0093] The operation method and steps are the same as those in Example 1 to evaluate the biological toxicity of heavy metal ions, the only change is: add Zn in the system 2+ to Zn 2+ The concentration in the system is 80μg / ml, detect 80μg / ml of Zn 2+ The biological toxicity of the size, by calculation, Zn 2+ The inhibition rate was 31.17%,

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Abstract

The invention discloses an electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor comprises a work electrode, a counter electrode, a reference electrode and a electrolytic cell; the work electrode is a polymer fixed microbial electrode, and the polymer fixed microbial electrode comprises an electrode and a polymer and bacteria mixed layer coated outside the electrode; and the material of the above polymer is gelatin or chitosan, the above bacteria comprise Escherichia coli and / or yeast, and the electrode is a glassy carbon electrode, a gold electrode, a platinum electrode or a boron-doped diamond film electrode. The invention also discloses an apparatus of the electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor can instantly monitor the water biotoxicity change and detect the water biotoxicity value, can realize instant, online and continuous detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and in particular relates to an electrochemical sensor and a device thereof which can be used for real-time monitoring and detection of water biological toxicity. Background technique [0002] With the development of modern industry, the increasing environmental pollution has caused a great impact on the water ecosystem, and its toxicity detection has become an important link in the evaluation of water environment quality. At present, the methods used to test the toxicity of pollutants mainly include physical and chemical methods and biological methods. Physical and chemical methods such as liquid chromatography and atomic absorption spectroscopy can accurately and quantitatively analyze the content of a certain pollutant or a certain type of pollutant; however, the comprehensive impact of pollutants on the ecosystem is often not a simple sum of the toxicity of each single substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/26
Inventor 只金芳李久铭王钰宁钱俊
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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