Method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine

A phthalate and phthalic acid technology, applied in the field of analytical chemistry, can solve the problems of reducing analysis efficiency, increasing analysis time, sample amount, solvent consumption, other analysis costs, and reducing sensitivity

Active Publication Date: 2017-03-22
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the metabolites of PAEs contain carboxyl groups, and phenolic compounds and estrogens contain phenolic hydroxyl groups, both are suitable for ionization in anion mode. The metabolites of PAEs contain carboxyl groups and are highly polar. , it is usually necessary to add formic acid or acetic acid to the mobile phase to improve the peak shape and optimize the chromatographic separation effect; unlike PAEs metabolites, phenolic compounds and estrogen metabolites are less polar than PAEs metabolites, so there is no need to add formic acid to the mobile phase or acetic acid, if formic acid or acetic acid is added to th...

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  • Method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine
  • Method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine
  • Method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine

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Embodiment 1

[0023] Such as figure 1 As shown, the high-pressure chromatographic pump pumps out the acidic mobile phase to bring the urine sample treatment solution containing PAEs, phenolic compounds and estrogen metabolites in the injector into the reversed-phase chromatographic column for chromatographic separation; After the impurities and their separation, they flow out from the chromatographic column in sequence; the acidic mobile phase flowing out from the chromatographic column is mixed with the high pH solution pumped by the syringe pump in the mixer, so that the pH value of the mobile phase flowing out of the mixer changes from acidic It is alkaline; the mobile phase passes through the capillary column and enters the electrospray ion source. In the anion mode, the compound to be tested is ionized and becomes an anion, and then enters the mass spectrometer through the ion extraction cone for analysis.

[0024] 1) Materials and methods

[0025] Instruments and reagents

[0026] A...

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Abstract

The invention relates to a method for simultaneous analysis of plastic additives, monomers and estrogen metabolites in urea, specifically to a method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine, belonging to the field of analytical chemistry. According to the method, after chromatographic separation of PAEs, phenolic compounds and estrogen metabolites in urea under acidic conditions, a solution with a pH value is injected into a mobile phase flowing out from a chromatographic column, so the mobile phase is adjusted an alkaline mobile phase before entering mass spectrometry; and thus, the ionizationoun efficiency of a to-be-detected substance in a mass spectrometric ion source is improved, phthalate metabolites, bisphenol A and estrogen in urine are analyzed at the same time in one sample introduction, and analysis efficacy is enhanced.

Description

technical field [0001] The present invention belongs to the field of analytical chemistry. The invention relates to a method for simultaneous analysis of plastic additives, monomers and estrogen metabolites in urine, and specifically relates to a method for rapid analysis of phthalate metabolites, bisphenol A and estrogen in urine. Background technique [0002] The prior art discloses that phthalate esters (PAEs) and phenolic compounds are important plastic additives and monomers respectively, and are widely used. In recent years, more and more studies have found that PAEs and phenolic compounds have endocrine disrupting effects, which have become the research focus of current non-persistent environmental endocrine disruptors in this field. Studies have shown that PAEs and phenolic compounds can interfere with the body's endocrine system through various ways, causing metabolic diseases related to endocrine. Epidemiological studies have found that the population is widely e...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/86
CPCG01N30/06G01N30/8634G01N30/88
Inventor 王和兴姜庆五何更生
Owner FUDAN UNIV
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