A rapid method for the analysis of phthalate metabolites, bisphenol A and estrogen in urine
A phthalic acid, rapid analysis technology, applied in the field of analytical chemistry, which can solve the problems of reducing sensitivity, increasing analysis time, sample consumption, solvent consumption, other analysis costs, and reducing analysis performance.
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[0023] Such as figure 1 As shown, the high-pressure chromatographic pump pumps out the acidic mobile phase to bring the urine sample treatment solution containing PAEs, phenolic compounds and estrogen metabolites in the injector into the reversed-phase chromatographic column for chromatographic separation; After the impurities and their separation, they flow out from the chromatographic column in sequence; the acidic mobile phase flowing out from the chromatographic column is mixed with the high pH solution pumped by the syringe pump in the mixer, so that the pH value of the mobile phase flowing out of the mixer changes from acidic It is alkaline; the mobile phase passes through the capillary column and enters the electrospray ion source. In the anion mode, the compound to be tested is ionized and becomes an anion, and then enters the mass spectrometer through the ion extraction cone for analysis.
[0024] 1) Materials and methods
[0025] Instruments and reagents
[0026] A...
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