1 results about "Sparfloxacin" patented technology

The present disclosure provides a gene delivery nanoparticle, and its preparation and application, pertaining to the technical field of biomedicine. In the preparation method, 2-((tert-butoxycarbonyl)amino)ethyl methacrylate as monomer, mPEG5k-RAFT as macromolecular chain transfer agent and azobisisobutyronitrile as initiator are subjected to reversible addition-fragmentation chain transfer polymerization in dimethylformamide, dialysis and freeze-drying to obtain an intermediate; the intermediate is dissolved in dichloromethane, added with trifluoroacetic acid and reacted to yield polyethylene glycol-poly(aminoethyl methacrylate); sparfloxacin is dissolved in a hydrochloric acid solution, and added with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and a solution of polyethylene glycol-poly(aminoethyl methacrylate) in dimethyl sulfoxide to produce a mixture; the mixture is subjected to dialysis and freeze-drying to obtain the final product polyethylene glycol-poly(sparfloxacin), which is placed in an aqueous solution to allow self-assembly, thereby forming the desired nanoparticle.