3 results about "Sparfloxacin" patented technology

The invention discloses a garnet nucleus carbon quantum dot fluorescent probe, a preparation method thereof and an application of the fluorescent probe in rapid detection of sparfloxacin. Green and environment-friendly waste garnet nucleus is selected as a carbon source, ammonium sulfate and europium nitrate are used as dopants, and DNW CDs with good luminescence stability and high solubility are synthesized through a one-step hydrothermal method; the method is used for rapidly detecting the content of sparfloxacin in an actual sample, resource recycling can be achieved while the residual level of sparfloxacin in food is monitored, and environmental pollution is reduced.

The present disclosure provides a gene delivery nanoparticle, and its preparation and application, pertaining to the technical field of biomedicine. In the preparation method, 2-((tert-butoxycarbonyl)amino)ethyl methacrylate as monomer, mPEG5k-RAFT as macromolecular chain transfer agent and azobisisobutyronitrile as initiator are subjected to reversible addition-fragmentation chain transfer polymerization in dimethylformamide, dialysis and freeze-drying to obtain an intermediate; the intermediate is dissolved in dichloromethane, added with trifluoroacetic acid and reacted to yield polyethylene glycol-poly(aminoethyl methacrylate); sparfloxacin is dissolved in a hydrochloric acid solution, and added with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and a solution of polyethylene glycol-poly(aminoethyl methacrylate) in dimethyl sulfoxide to produce a mixture; the mixture is subjected to dialysis and freeze-drying to obtain the final product polyethylene glycol-poly(sparfloxacin), which is placed in an aqueous solution to allow self-assembly, thereby forming the desired nanoparticle.

The invention provides a gene delivery nanoparticle and a preparation method and application thereof, and relates to the technical field of biological medicines.The preparation method comprises the steps that 2-((t-butyloxycarboryl) amino) ethyl methacrylate serves as a monomer, mPEG5k-RAFT serves as a macromolecular chain transfer agent, azodiisobutyronitrile serves as an initiator, a reaction is conducted in a dimethylformamide solvent, and the gene delivery nanoparticle is obtained. Carrying out reversible addition-fragmentation chain transfer polymerization reaction, dialyzing and freeze-drying to obtain an intermediate; dissolving the intermediate in dichloromethane, adding trifluoroacetic acid, and reacting to obtain a polymer polyethylene glycol-polymethylacrylic acid amino ethyl ester; the preparation method comprises the following steps: dissolving sparfloxacin in a hydrochloric acid solution, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, dissolving polyethylene glycol-poly (aminoethyl methacrylate) in dimethyl sulfoxide, adding the solution into the aqueous solution, dialyzing and freeze-drying to obtain the final product polyethylene glycol-polysparfloxacin. The preparation method comprises the following steps: putting polyethylene glycol-polysparfloxacin into an aqueous solution, and carrying out self-assembly to form nano particles.