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Method for determining 10 quinolone antibiotics in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry

An ultra-high performance liquid phase and tandem mass spectrometry technology, applied in the field of food testing, can solve the problem of limited research objects, achieve the effects of improving extraction efficiency, improving analysis sensitivity, and shortening analysis time

Inactive Publication Date: 2019-03-01
SHANDONG INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0003] In recent years, most of the research on the analysis of residues of quinolone antibiotics is limited to the field of animal food, while there are few researches on the analysis of residues in vegetables, especially bean sprouts, and the research objects are limited to enrofloxacin, ciprofloxacin and ciprofloxacin. , norfloxacin and one or a few compounds, the method has certain limitations

Method used

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  • Method for determining 10 quinolone antibiotics in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining 10 quinolone antibiotics in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining 10 quinolone antibiotics in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry

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Embodiment 1

[0026] 1 Experimental part

[0027] 1.1 Instruments, reagents and materials

[0028] AB Sciex 5500 tandem mass spectrometer (AB Sciex, USA); AQUITY UPLC I-Class ultra-high performance liquid chromatography (waters, USA); MilliQ deionized water generator (Millipore, USA); N-EVAP48 nitrogen blowing instrument (USA Orangaomation company), IKA MS3 vortex mixer; 0.22 μm organic phase needle filter (China ANPEL company).

[0029] Acetonitrile (Merck, USA); formic acid (Fisher Scientific, USA); PRIME HLB purification column (100mg, 6mL, waters, USA); enrofloxacin (CAS NO.93106-60-6), ciprofloxacin (CAS NO.85721-33-1), norfloxacin (CAS NO.70458-96-7), pefloxacin (CAS NO.70458-92-3), ofloxacin (CAS NO.82419-36- 1), sarafloxacin (CAS NO.98105-99-8), dafloxacin (CAS NO.112398-08-0), sparfloxacin (110871-86-8), fleroxacin (CAS NO. 79660-72-3), lomefloxacin (CAS NO.98079-51-7) are all from Dr.Ehrenstorfer GmbH, Germany; the chemical structural formulas of the quinolone core and 10 kinds...

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Abstract

The invention discloses a method for determining 10 quinolone antibiotics in bean sprouts by an ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps of: preprocessing; preparing a standard solution; obtaining a liquid chromatography mass spectrogram and a regression equation; and analyzing and detecting samples by the ultra-high performance liquid chromatography-tandem mass spectrometry. A ultra-high performance liquid chromatography-electrospray tandem mass spectrometry is adopted in this study to carry out an analysisand determination of enrofloxacin, ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, sarafloxacin, danofloxacin, sparfloxacin, fleroxacin and lomefloxacin in the bean sprouts. Sample extraction conditions, purification means, liquid chromatography-mass spectrum parameters and the like are optimized and the matrix effect of the method is evaluated. The result shows that an establishment of the method solves the problem of a simultaneous determination of various quinolone antibiotics in the bean sprouts and provides a technical reference for risk assessment and government supervision.

Description

technical field [0001] The invention relates to a method for determining 10 quinolone antibiotics in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of food detection. Background technique [0002] Bean sprouts are a traditional vegetable in our country, rich in protein, B vitamins and trace elements. Because of its rich nutrition and easy cooking, it is deeply loved by the common people. Since 2010, reports on "poisonous bean sprouts" have emerged one after another. Some unscrupulous traders have added enrofloxacin and other quinolones to the production process of bean sprouts to achieve the purpose of antibacterial and sterilization. Long-term consumption of this bean sprouts will cause certain harm to the human body, produce drug resistance or some toxic and side effects. Announcement No. 235 of the Ministry of Agriculture of my country clearly stipulates that the maximum residue limit of quinolon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 宿书芳程志郑红刘艳明魏莉莉丁一薛霞
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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