163 results about "Quinolone antibiotic" patented technology

The present invention provides pharmaceutical aerosols which are useful for the prevention or treatment of infectious diseases of the airways, such as the lungs, the bronchi, or the sinunasal cavities. The aerosols comprise an active agent selected from the group of quinolone antibiotics. Also disclosed are liquid and solid compositions suitable for being converted into the aerosols, and kits comprising such compositions.

Disclosed are novel formulations for the treatment of otic infections in an animal comprising a triazole anti-fungal compound, a quinolone antibiotic and a corticosteroid such as mometasone furoate monohydrate.

The present invention provides methods for identifying target genes whose partial or complete functional inactivation potentiates the activity of an antibiotic agent, e.g., a quinolone antibiotic. The invention further provides methods for identifying agents that modulate expression of target genes or that modulate activity of expression products of target genes. Agents identified according to various methods of the invention potentiate the activity of antibiotics such as quinolones, aminoglycosides, peptide antibiotics and β-lactams. Also provided are agents that suppress and / or retard resistance to antibiotics. The inventive methods provide potentiating agents and compositions comprising potentiating agents and antibiotics. Such agents and compositions can be used for inhibiting growth or survival of a microbial cell or of treating a subject suffering from or susceptible to a microbial infection.

The invention relates to a method for preparing a magnetic charcoal material, a device adopting the method and application. The method comprises the following steps that sewage treatment plant sludgeis subjected to high-temperature anaerobic pyrolysis, and sludge charcoal is obtained; then dry fine grinding is carried out, and charcoal powder is obtained; the sludge charcoal powder is subjected to dry magnetic separation, a magnetic material is obtained, granules obtained after humidifying and pelleting are subjected to high-temperature sintering in protective atmosphere, and sintered materials are obtained; furnace cooling is carried out, and the magnetic charcoal material of a porous structure is obtained. According to the method, the magnetic material is separated out of the sludge pyrolysis product charcoal and is used for preparing the magnetic charcoal material, the magnetic charcoal material is used for efficiently adsorbing and removing quinolone antibiotics such as ciprofloxacin, recycling utilization of the sludge charcoal is achieved, the preparation cost of the adsorption materials is reduced, the process is simple, high practicality is achieved, and good economic benefits and environmental benefits are achieved.

The invention relates to a method for separation enrichment and detection of trace fluoroquinolone antibiotic residues in a water environment. According to the method, 2.0-4.0L of a water sample is subjected to solid-phase extraction, and then analysis and detection are carried out by a liquid chromatography-mass spectrometry instrument. Being able to well separate and detect a plurality of fluoroquinolone antibiotic substances, the invention has the advantages of low detection limit, high recovery rate, high sensitivity, good selectivity and simple operation, etc., and has substantial effects in the detection study of trace fluoroquinolone antibiotic substances in the water environment.

The invention provides a method for removing antibiotic contamination by adsorption of copper sulphide, and belongs to the field of water pollution control. The method comprises the steps of filtering waste water containing antibiotic contamination, adjusting pH to 5-9; and filtering the waste water processed in the step (1) through a bed layer containing the copper sulphide, wherein the antibiotic contamination is any one of tetracyclines, penicillins, macrolides, and sulfonamide fluoroquinolone antibiotic. Compared with the traditional removal technology of the antibiotic in a water body, the method disclosed by the invention has the beneficial effects that 1 the antibiotic contamination in the water body can be selectively complexed and removed; and the effluent concentration can be less than 1mug / L; 2, the adsorbing capacity is large; and the adsorbing capacity of copper sulphide can be up to 500mg / g; and 3, the method is fast in adsorption rate, easy to regenerate, simple and convenient to operate, low in cost and free of secondary pollution.

The invention discloses a quinolone antibiotic degrading bacterium and application thereof and belongs to the technical field of biological treatment of environmental pollutants. The degrading bacterium is obtained by domestication and purification of livestock manure, and is named as Ochrobactrum sp. JOB, and was preserved in Guangdong Microbiological Culture Collection Center (GDMCC), Guangdong Province Institute of Microbiology on the fifth floor, No.59 Building, No.100 Courtyard, Xianlie Middle Road, Guanzhou City on December 21st, 2016, with a preservation number of GDMCC NO:60132. The degrading bacterium disclosed by the invention is prepared into bacterium suspension or an immobilized microbial agent which can be used for effectively removing quinolone antibiotic pollution in different environmental media such as a water body and soil; compared with a physical absorption method, a chemical degradation method and the like, the quinolone antibiotic degrading bacterium has the advantages of high efficiency, energy conservation, environment friendliness and the like; particularly, a bacterial body is immobilized by using an embedding agent, so that the life of the bacterial body is prolonged; the microbial agent has an excellent action effect and high ability of adaptation to environment.

Belonging to the technical field of water treatment, the invention relates to preparation of a magnetic reduced graphene oxide composite material. The method includes: (1) placing graphene oxide into ethylene glycol to undergo ultrasonic treatment; (2) dissolving sodium citrate in ethylene glycol to undergo heating reaction; (3) mixing FeCl3.6H2O with urea, then dissolving the mixture into a sodium citrate / ethylene glycol solution under magnetic stirring to obtain an ethylene glycol mixed solution of the iron salt and urea; (4) stirring and mixing the graphene oxide / ethylene glycol mixed solution and the ethylene glycol mixed solution of the iron salt and urea obtained in the preceding step to undergo reaction, then transferring the reaction product into a reaction kettle to undergo heating reaction and cooling; and (5) subjecting the obtained material to centrifugation, washing and filtering, and freeze drying, thus obtaining the product involved in the invention. The magnetic reduced graphene oxide prepared by the method provided by the invention has the advantages of pure crystal phase, difficult agglomeration, and high magnetization degree. Also, the process is simple and has low requirements for production equipment. The invention also relates to a method for removing quinolone antibiotics from water by the composite material.

The invention discloses primers and a kit for detecting the quinolone drug-resistance genes of bacteria, and belongs to the technical field of microbiological detection. The sequences of the primers for detecting the quinolone drug-resistance genes of bacteria provided by the invention are shown in SEQ ID NO: 1-6, and the three primer pairs are capable of specifically amplifying the three quinolone drug-resistance genes of qnrA, qnrB and qnrS of multiple bacteria. The invention further discloses a kit for detecting the quinolone drug-resistance genes of bacteria, wherein the kit contains an EvaGreen fluorescent dye, and is capable of rapidly and accurately detecting the quinolone antibiotic-related drug-resistance genes of bacteria. The primers and the kit disclosed by the invention have the advantages of simplicity and convenience in operation, high specificity, high sensibility, low cost, high flux and the like, and can be used for rapidly detecting quinolone antibiotic-related drug-resistance genes of clinic pathological bacteria and providing a reference for clinic treatment.

The invention discloses a preparation method of a microwave-assisted magnetic hollow Zn / Co zeolite imidazole nanocage material. The preparation method comprises the steps: adopting Nano Fe3O4 as a seed crystal, adding cobalt nitrate hexahydrate and 2-methylimidazole and zinc nitrate hexahydrate successively, performing rapid self-assembly based on seed crystal induction so as to form a double-shell Zn / Co-ZIF crystal and form a magnetic double-shell nano crystal Fe3O4@ZIF-67@ZIF-8, utilizing methanol to absorb the microwave energy under the assistance action of microwaves, and transferring energy quickly to an inner shell ZIF-67 with poor chemical stability so as to bring vibration and cracking of metal organic skeleton ZIF-67 bonds, achieve etching of the inner shell and obtain Fe3O4@Zn / Co-ZIF. The material has a hollow cage-shaped morphology, a large specific surface area, a complete crystal form, multiple pores, high chemical stability and good magnetic properties for convenient magnetic separation. The material has an efficient and high-throughput adsorption for antibiotic quinolone drugs, has another advantage of easy separation from the antibiotic quinolone drugs, can be regenerated and reused at least 8 times, has excellent reproducibility, and has easy realization of high-efficiency removal of high-residue quinolone antibiotics in a large-scale water environment.

The invention belongs to the technical field of degradation of soil organic pollutants, and discloses a composite microbial preparation for degrading quinolones antibiotics and a preparation method and application. The preparation method comprises the steps of mixing quinolones antibiotics degradation bacterial liquid with energy supply bacterium bacterial liquid, magnetic medium bacterium bacterial liquid, a surfactant and a fermentation medium to obtain mixed liquid; and performing fermentation to obtain the composite microbial preparation for degrading the quinolones antibiotics. Energy supply bacteria which are adopted enable the composite microbial preparation to have high colonization and multiplication capacity in in-place edatope. The surfactant and the magnetic medium bacteria, which are adopted, enable the composite microbial preparation to have low moisture tension and antibiotics polarity. The prepared composite microbial preparation is high in activity, and high in environmental suitability. The composite microbial preparation is used for degradation of the quinolones antibiotics in soil, and is broad in scope of application and high in degradation efficiency.

The invention provides an aspartate quinolone antibiotics water-soluble salt and the injection formulation thereof, including sparfloxacin, gatifloxacin, rufloxacin, pefloxacin, tosufloxacin, moxifloxacin, and so on; the invention improves the water solubility of quinolone antibiotics and enhances the anti-bacterial effect of quinolone antibiotics. Compared with the oral liquid of quinolone drugs of the prior art, the invention has the advantages that: water solubility of the drug is good, as the drug enters the blood directly, the invention can not only achieve treatment function rapidly, but can also be absorbed by the human body fully, and the invention has significant effects in the two aspects of fast onset of action and low consumption. Compared with the injection of the quinolone drugs of the prior art, the invention has the advantages that: due to the existence of the L-aspartate, the antibacterial activity of quinolone drugs can be enhanced.

The invention belongs to the field of water supply and sewerage works / environmental engineering, and discloses a method for targeted degradation of quinolone antibiotics in water and a multiband ultraviolet (UV) radiation device used for the method. The method for targeted degradation of the quinolone antibiotics in water includes the following steps that wastewater containing the quinolone antibiotics is added with a H2O2 aqueous solution and / or aqueous solution containing S2O8<2>, and then the wastewater is degraded under the multiband UV radiation action, so that a water body for targeted degradation of the quinolone antibiotics is obtained. The invention further provides the multiband UV radiation device used for the method. According to the method, the multiband UV radiation device independently designed and developed is used as the main body to be combined with the H2O2 and S2O8<2>, so that a composite UV degradation technology is formed, and the technology can be used in a water supply treatment plant or wastewater treatment plant; and electric energy serves as the driving energy, UV radiation serves as the excitation energy, the H2O2 or S2O8<2> serves as the oxidizing agent donor, efficient targeted degradation of ciprofloxacin is realized, and finally the antibacterial activity and toxicity are weakened.

The invention relates to a fluorescent microsphere immune test paper card for detecting quinolone antibiotics and preparation and detection methods and belongs to the field of immunological detection.The test paper card comprises a card shell and a test paper strip. The test paper strip comprises a bottom plate, and a water absorption pad, a detection pad, a combining pad and a sample pad successively overlapped and adhered to the bottom plate; the detection pad is a nitrocellulose membrane provided with a quality control line C and a detection line T, the quality control line C is coated with a goat anti mouse monoclonal antibody and the detection line T is coated with an enrofloxacin-bovine serum albumin conjugate; the combining pad is a glass cellulose membrane of the enrofloxacin monoclonal antibody embedded with a time-resolved fluorescent microsphere label; the sample pad is a dried glass cellulose membrane immersed in a sample treatment liquid; the bottom plate is a PVC plate which does not absorb water, and the water absorption pad is water absorbing filter paper. The test paper card prepared is good in stability, high in sensitivity and high in specificity, can detect 16quinolone antibiotics, and can achieve the purpose of quantitative and batch detection of enrofloxacin quickly through fluorescence signals.

The present invention relates to a biochemical treatment reactor for waste water containing quinolone antibiotics, the reactor is composed of CAF (Composite Anaerobic Filter) and FBBR (Fixed Bed Membrane Bioreactor). Such pollutants are decomposed into small molecular compounds, and then degraded by microorganisms in the aerobic reactor, so as to achieve the purpose of removing such pollutants. By improving the traditional reactor, the present invention not only solves the problem that antibiotic wastewater containing quinolone compounds is difficult to biodegrade, but also solves the problem of mud run-off and difficult acclimation in traditional reactors, and opens up a new path for the treatment of this type of antibiotic wastewater new way.

An antibiotic polymer combination / antibiotics polymer combination that ensures the continuous release of antibiotics over a period of several days under physiological conditions, and that can be used in human and veterinary medicine. Surprisingly, one or more antibiotic salts, which are sparingly soluble in water, from the groups comprising aminoglycoside antibiotics, lincosamide antibiotics, tetracycline antibiotics, glycopeptide antibiotics, quinolone antibiotics and chlorhexidine, are suspended in homogeneous polymer mixtures, which comprise one or more hydrophobic, nonionic polymers from the groups comprising poly(vinyl, chloride), post-chlorinated poly(vinyl chloride), poly(vinylidene chloride), poly(vinyl fluoride), poly(vinylidene fluoride) and copolymers comprising vinyl chloride and one or more nonionic monomers, and which comprise one or more hydrophilic polymers from the groups comprising polyethers, and this suspension forms composites that exhibit the release of an active ingredient over a period of days in an aqueous medium.

The invention provides a magnetic nano-microsphere and a method for extracting and analyzing five trace quinolone antibiotics in a water body by virtue of the magnetic nano-microsphere. The magnetic nano-microsphere is Fe3O4@Cys@MIL125-NH2. By taking a magnetic solid phase extraction technique as the basis and the magnetic nano-microsphere as an adsorbent, the quinolone antibiotics in a to-be-tested water body are pretreated and enriched, so that the sensitivity of the method for detecting the trace quinolone antibiotics in the water body is greatly improved.

The invention discloses a yellow fluorescence carbon dot, a preparation method of the yellow fluorescence carbon dot, and an application of the yellow fluorescence carbon dot to label-free detection of quinolone antibiotics. The carbon dot is a yellow fluorescence carbon dot taking o-phenylenediamine and aminobutyric acid as the raw materials and prepared through a one-step hydro-thermal synthesismethod. The preparation method of the carbon dot is simple and convenient, and the prepared carbon dot is stable in optical property and good in water solubility and dispersity. The carbon dot, as afluorescence probe, is used to rapidly sensitively detect the quinolone antibiotics without label due to the special surface structure. When the quinolone antibiotics exist, the fluorescence of the carbon dot is effectively quenched by the quinolone antibiotics, and the content of the quinolone antibiotics in a sample is detected according to the variation degree of the fluorescence. Compared withthe traditional method, the provided quinolone antibiotics detection method is simple, convenient and fast, no label is extraordinarily modified, the sample is directly detected, and the method has more advantages in the practical application.

The invention belongs to the technical field of food detection. The invention relates to a magnetic nano composite material as well as preparation and application thereof in food detection. The invention specifically relates to an application of an ionic liquid dispersion liquid-liquid microextraction and magnetic solid phase extraction (DLLME-MSPE) two-step extraction technology in selective extraction and trace determination of quinolone antibiotics in honey and milk in combination with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS / MS). The invention furtherprovides a sensitive and efficient method for detecting quinolone antibiotics in honey and milk. The saturation magnetization intensity of the magnetic multi-walled carbon nanotube coated with the silicon dioxide modified by the magnetic nano composite material chitosan prepared by the invention is 22.9 emu / g or above. The magnetic nano composite material can be used as a magnetic solid-phase extraction adsorbent for simultaneous quantitative analysis of various quinolone drugs.

The invention discloses a near infrared antibiotic fluorescent probe detecting agent and a preparing method and application thereof. The near infrared antibiotic fluorescent probe detecting agent comprises a fluorescent nanometer material. The excitation spectrum of the fluorescent nanometer material and the absorption spectrum of antibiotics are at least partly overlapped so that the excitation wavelength of the fluorescent nanometer material can be absorbed by the antibiotics and the fluorescence of the fluorescent nanometer material can be quenched. The fluorescent nanometer probe agent which is high in selectivity, high in detection sensitivity, simple and feasible and can be used for detecting tetracycline and carbostyril antibiotics is established according to the principle of the internal filtering effect formed between near infrared carbon quantum dots and other fluorescent nanometer materials and the antibiotics, the selectivity of the fluorescent nanometer probe is improved,the technical problem that an existing antibiotic detecting probe is not enough in sensitivity and complicated in detecting process can be solved, the detecting agent has a remarkable effect when usedfor detecting actual samples, and the detecting agent can be easily designed into a sensor for detecting the antibiotics in the actual samples on site.

The invention relates to a method for quinoxalone antibiotics multi-residue enzyme-linked immune detection, and belongs to the technology field of the antibiotics residue detection method. In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng / ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng / ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng / ml. In addition, the ELISA method is also evaluated in the aspects of specificity, accuracy and sensitivity, thereby conforming to the detection requirements for the multi-residue ELISA method.

The invention discloses a culture medium and a preparation method for selective separation culture extensively drug-resistant pseudomonas aeruginosa. Culture supernatant fluid and an antibiotic combination for pseudomonas aeruginosa in a logarithmic phase are added into an NAC basal culture medium. The culture supernatant fluid contains quorum sensing substances secreted by pseudomonas aeruginosa, the quorum sensing substances can accelerate growth of bacteria and promote secretion of aeruginosa pigments, and the detection time is greatly shortened. The antibiotic combination comprises carbapenems antibiotics, aminoglycoside antibiotics and quinolone antibiotics, the additive amount of the carbapenems antibiotics is 0.016 g / L, the additive amount of the aminoglycoside antibiotics is 0.016 g / L, and the additive amount of the quinolone antibiotics is 0.016 g / L, the antibiotic combination can specifically inhibit growth of sensitive pseudomonas aeruginosa strains and growth of single-drug resistant pseudomonas aeruginosa strains, the purpose of selective separation culture of extensively drug-resistant pseudomonas aeruginosa is achieved, and specificity and sensitivity are high. Moreover, the culture medium can be stored for a long time and can be effectively applied to quick detection of extensively drug-resistant pseudomonas aeruginosa in clinical samples.

The invention discloses a method for determining 10 quinolone antibiotics in bean sprouts by an ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps of: preprocessing; preparing a standard solution; obtaining a liquid chromatography mass spectrogram and a regression equation; and analyzing and detecting samples by the ultra-high performance liquid chromatography-tandem mass spectrometry. A ultra-high performance liquid chromatography-electrospray tandem mass spectrometry is adopted in this study to carry out an analysisand determination of enrofloxacin, ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, sarafloxacin, danofloxacin, sparfloxacin, fleroxacin and lomefloxacin in the bean sprouts. Sample extraction conditions, purification means, liquid chromatography-mass spectrum parameters and the like are optimized and the matrix effect of the method is evaluated. The result shows that an establishment of the method solves the problem of a simultaneous determination of various quinolone antibiotics in the bean sprouts and provides a technical reference for risk assessment and government supervision.

The invention discloses a preparation method of a fluoroquinolone antibiotic adsorbent. The preparation method comprises the following steps: 1) dissolving metal salt and an organic ligand in N,N-dimethylformamide to obtain a solution A; 2) transferring the solution A into a hydrothermal reaction kettle, and reacting for 20-30h at 100-160 DEG C; cooling to room temperature; performing centrifugation, washing and drying to obtain an intermediate product; 3) dissolving the intermediate product and cobalt sulfate in an organic solvent, performing magnetic stirring and cooling to room temperature;performing centrifugation and drying to obtain the quinolone antibiotic adsorbent. The pore diameter of the adsorbent is 1.5-3.2nm, and the specific surface area is 698-1,143m<2> / g. The preparation method disclosed by the invention has the advantages of simple preparation steps, low cost, high removal efficiency of quinolone antibiotic pollutants and broad application prospect.

The invention provides a three dimensional-similar electro-Fenton water treatment system and a method used for degrading carbostyril antibiotics. The three dimensional-similar electro-Fenton water treatment system comprises a reactor; the reactor comprises a three dimensional-similar electro-Fenton reaction zone and a biological filter material reaction zone; iron pyrite roasting slag based Co / Fe<0> particle electrodes are arranged at the three dimensional-similar electro-Fenton reaction zone; a biological filtering material is introduced into the biological filter material reaction zone; wastewater is subjected to common treatment by microorganisms on the biological filtering material so as to reduced COD and ammonia nitrogen to a certain degree, and then is delivered into the three dimensional-similar electro-Fenton reaction zone for deep treatment of substances difficult to be biodegraded; a persulfate is introduced into the reactor using an agent adding device, wastewater is delivered into the reactor, and is mixed with the persulfate. Under the action of particle electrode-loaded Fe<0> at the three dimensional-similar electro-Fenton reaction zone, the persulfate is activated so as to generate sulfate free radicals, hydroxyl free radicals are generated via electrocatalysis, and the sulfate free radicals and the hydroxyl free radicals are used for degrading pollutants in waste effectively.

The present invention relates to an antibiotic-polymer complex that ensures continuous release of antibiotics for several days under physiological conditions and can be used in human and veterinary medicine. The antibiotic-polymer composite of the present invention is characterized in that it contains one or more hydrophobic polymers selected from the group consisting of polymethacrylates, polyacrylates, methacrylate-acrylate copolymers and a Or in a homogeneous mixture of polymers composed of hydrophilic polymers such as polyethers, one or more substances selected from the group consisting of aminoglycoside antibiotics, lincosamide antibiotics, tetracycline antibiotics, and quinolone antibiotics are suspended in water. A poorly soluble antibiotic, a water-soluble antibiotic selected from aminoglycoside antibiotics, lincosamide antibiotics, β-lactam antibiotics and tetracycline antibiotics as needed, and an organic auxiliary as needed material and causing the suspension to form a composite.

The invention provides a preparation method of functional magnetic dipyridyl type coordination polymer and an application thereof. The prepared functional magnetic dipyridyl type coordination polymer Cu-BPD / Fe3O4 / SDS is applied to absorb and remove quinolone antibiotics in the water. The functional magnetic dipyridyl type coordination polymer used as the absorbent is used for removing remove quinolone antibiotics in the water. The absorbent is possessed of relatively high removal rate and rapid magnetic separation ability, and can be repeatedly used and so on. The preparation method is low in cost, gentle in reaction, and simple in operation. As for the quinolone antibiotics, the absorbent has the advantages of being good in thermal stability, quick absorbing rate, high absorbing capacity, rapid magnetic separating ability, regeneration and recyclability.

The invention relates to a method for restoring antibiotics-polluted wastewater, which comprises the following steps: airing soil, then screening with a sieve of 15-30 meshes, and firing for 15-40 minutes at 400-600 DEG C to obtain a restorative agent; and feeding the restorative agent into the polluted wastewater with antibiotics, thus finishing the remediation within 5-10 hours. The method provided by the invention is efficient in removing antibiotics, especially tetracycline and quinolone from the polluted wastewater, with the removal rate up to 99.9% within 5 hours, capable of removing all the antibiotics within 6-10 hours. The restored water may meet relevant national standards for quality of water.