54 results about "Quinolone antibiotic" patented technology

The present invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation. The low-temperature-resistant multifunctional bacteria is the strain TLT1 of the genus Tularemia, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on August 9, 2023, with the deposit number: CGMCC No: 40754. The bacterial agent of the present invention can be used to stabilize zinc in the soil environment, which helps to enrich the database of bacterial strains that stabilize heavy metals and have the ability to degrade quinolone antibiotics. The present invention prepares the purified strain into a bacterial suspension, which can effectively stabilize zinc in different environmental media such as water and soil under low-temperature conditions, and has the ability to degrade enrofloxacin in different environmental media such as water and soil. Compared with methods such as chemical oxidation, this method is more efficient, less expensive, and has no secondary pollution, providing an effective biological-non-biological combined remediation technology for the remediation of zinc-enrofloxacin complex-contaminated soil and water.

The invention relates to the technical field of pathogenic bacterium detection, in particular to a method and a kit for detecting helicobacter pylori gene mutation in excrement. According to the technical scheme, the kit for detecting helicobacter pylori gene mutation in excrement is developed by combining multiple advanced technical means such as ARMS-PCR design, blocking primers, locked nucleic acid modification (peptide nucleic acid modification), MGB probes, nested PCR design and degenerate base design. The kit can detect nine gene mutation sites related to the drug resistance of helicobacter pylori at one time, and has a wide coverage range. The kit in the scheme can be used for detecting the drug resistance of quinolone antibiotics and / or clarithromycin antibiotics of helicobacter pylori, and the technical problem that a high-specificity and high-sensitivity excrement helicobacter pylori gene mutation detection method is lacked in the prior art is solved. The method is simple and convenient to operate and can be completed within 1-2 hours, the detection cost is reduced, and the method has ideal application and popularization prospects.

The invention belongs to the technical field of microorganisms, and particularly discloses a fluoroquinolone antibiotic efficient degrading bacterium and application thereof. The fluoroquinolone antibiotic degrading bacterium is Kocuria sp. X03, the preservation number of the Kocuria sp. X03 is CCTCC NO: M 2025522, the invention provides the fluoroquinolone antibiotic degrading bacterium Kocuria sp. X03 separated from soil, the Kocuria sp. X03 can efficiently degrade fluoroquinolone antibiotics, an antibiotic degrading strain library is enriched, biotransformation mechanism research and bioremediation application are promoted, and the application prospect is wide. The strain can be used for degrading and eliminating livestock and poultry manure and residual antibiotics in farmland soil, and has certain research and application values.

The invention discloses an Ag-TiO2-Ag surface enhanced Raman scattering substrate, preparation thereof and application of the Ag-TiO2-Ag surface enhanced Raman scattering substrate in antibiotic residue detection or degradation, and belongs to the technical field of material preparation and analysis detection. The preparation method of the substrate comprises the following steps: (1) preparing a tetrabutyl titanate-ethanol mixed solution and a silver nitrate-ethanol-nitric acid mixed solution, regulating the molar ratio of Ag to Ti, and then carrying out hydrolysis reaction; (2) carrying out hydrothermal reaction and vacuum drying on the hydrolysis product to obtain a precursor; and (3) carrying out roasting treatment on the precursor, so as to obtain the Ag-TiO2-Ag SERS substrate. The prepared substrate can realize high-sensitivity detection of quinolone antibiotics in a water body, the detection limit reaches 10 <-11 > mol / L level, meanwhile, the substrate has photocatalytic degradation capacity, and the degradation rate within 60 minutes exceeds 90%. The method adopts a conventional process, does not need special equipment, and is suitable for industrial large-scale production and environmental pollutant detection and treatment.

The invention discloses a MoO3 / TiO2 heterojunction material with a photocatalytic memory effect and application of the MoO3 / TiO2 heterojunction material in degradation of quinolone antibiotics, and belongs to the technical field of inorganic non-metallic material preparation, solar energy utilization and environmental protection. A molybdenum source precursor solution and a titanium source precursor solution are adopted to react, polyvinylpyrrolidone is used for modifying the surface of the material, the MoO3 / TiO2 heterojunction material is obtained, and the material can generate photochromic and photocatalytic memory effects under the pre-illumination effect. The memory effect provides continuous driving force for the catalytic reaction, and the activity of the material in the dark-state catalytic process is remarkably enhanced. The MoO3 / TiO2 heterojunction material disclosed by the invention shows excellent catalytic degradation performance in practical application, and particularly shows extremely high catalytic efficiency in the aspect of water pollution control.

The invention discloses a preparation method and application of UiO-66 / MWCNTs-COOH / SA composite gel microspheres, and belongs to the technical field of composite material preparation. The preparation method comprises the following steps: mixing a zirconium source, terephthalic acid, carboxylated multi-walled carbon nanotubes, a solvent and glacial acetic acid, and heating for reaction to obtain a UiO-66 / MWCNTs-COOH composite material; adding sodium alginate according to a certain proportion, and cross-linking the SA-UC gel microspheres with the sodium alginate to form SA-UC composite gel microspheres. The SA-UC composite gel microspheres have high adsorption capacity on quinolone antibiotics, are easier to carry out solid-liquid separation, and have the advantages of high recycling rate and good recycling performance. In addition, when the SA-UC composite gel microspheres adsorb norfloxacin in solutions with different ionic strengths, the adsorption capacity can still reach about 90%, and the anti-ionic interference capability is high.

The invention relates to the technical field of adsorption materials. The invention provides a preparation method of a molybdenum disulfide loaded biochar binary adsorbent, which takes modified straw biochar (MBC) as a carrier, and successfully synthesizes a MoS2 nano-powder loaded biochar (MBC) binary adsorbent through an ultrasonic method and a one-step hydrothermal method. According to the technical scheme, the acting force between the prepared binary adsorbent and antibiotic molecules is higher, and the adsorbability to the antibiotic molecules is higher; wherein the introduction of MBC also effectively improves the disadvantage of easy hydrophobic agglomeration of MoS2, at the same time, the equilibrium adsorption time is shortened by nearly 3 times, the equilibrium adsorption capacity is improved by about 15%, and the quinolone antibiotics can be effectively removed under neutral and acidic conditions, so that the method has wide application prospects in the aspect of removing quinolone antibiotic pollutants.

The invention belongs to the technical field of microbial degradation, and particularly relates to pandoraea sp., a microbial agent and application of the pandoraea sp. In degradation of quinolone antibiotics. The Pandoraea sp. Is named as AYGW-1, and is preserved in the China Center for Type Culture Collection on March 10, 2025, and the preservation number is CCTCC M 2025415. The Pandoraea sp. Is named as Pandoraea sp. The Pandoraea sp. AYGW-1 has the function of degrading the quinolone antibiotics, provides an effective biodegradation scheme for treatment of pollution of the quinolone antibiotics in the environment, especially in a water body, and also provides a safe and environment-friendly solution for the residue problem of quinolone antibiotic drugs.

The embodiment of the invention provides a Fe3O4 (at) COF electrochemical sensor based on a covalent organic framework, and a preparation method and application thereof. The electrochemical sensor comprises a base electrode with the surface modified with a solid film; the solid film is a Fe3O4 (at) COF composite material; the Fe3O4-coated COF composite material comprises an inner core formed by ferroferric oxide with a porous structure and a COF shell layer attached to the surface of the inner core, the COF shell layer is a covalent organic framework layer formed by copolymerization of an aromatic aldehyde compound and a polyamino aromatic compound. According to the Fe3O4 (at) COF electrochemical sensor based on the covalent organic framework, the preparation method and the application provided by the invention, quinolone antibiotic molecules such as norfloxacin and the like can be detected, and the detection limit can reach 1.927 * 10 <-13 > mol / L; and the method can be widely applied to detection of quinolone antibiotics such as norfloxacin in milk and environmental water samples.

The invention discloses a silver nanoparticle / polyvinyl alcohol hydrogel material, a preparation method thereof and application of the silver nanoparticle / polyvinyl alcohol hydrogel material in antibiotic detection. The silver nanoparticle / polyvinyl alcohol hydrogel material comprises monodisperse silver nanoparticles (AgNPs) and polyvinyl alcohol hydrogel, the monodisperse silver nanoparticles are embedded into the polyvinyl alcohol hydrogel, and an AgNPs / PVA composite substrate is formed through calcium ion induced aggregation and freeze thawing crosslinking. The silver nanoparticle / polyvinyl alcohol hydrogel material is used as a hydrogel SERS (Surface Enhanced Raman Scattering) chip for detecting residual veterinary drugs in animal-derived foods, shows good linear response to different antibiotics, has an adding standard recovery rate of 76-86%, is low in cost, and has extremely high sensitivity and relatively strong reproducibility, stability and uniformity.

The invention belongs to the technical field of natural pharmaceutical chemistry and medical application, and discloses a quinolone antibiotic synergist and application thereof in preparation of a medicine for treating bacterial infection. The structural formula of the quinolone antibiotic synergist is shown in the specification. The quinolone antibiotic synergist can effectively inhibit the activity of a staphylococcus aureus efflux pump, and the survival rate of mice infected with staphylococcus aureus can be remarkably increased when the quinolone antibiotic synergist is combined with quinolone antibiotics for use.

The application provides a method for treating fluorine-containing quinolone antibiotic wastewater by using an electrochemical membrane reactor, and belongs to the technical field of antibiotic wastewater treatment. The fluorine-containing quinolone antibiotic wastewater is introduced into the electrochemical membrane reactor for electrolysis treatment, wherein the electrochemical membrane reactor has an anode membrane and a cathode membrane, and the anode membrane is a titanium-based electrocatalytic membrane loaded with an electrocatalyst. The specific surface area of the anode membrane can be effectively increased by doping boron elements, the stability of the electrode membrane is improved, and then the electrocatalytic activity of the anode membrane is improved, and the degradation rate reaches 100% after electrolysis for 1h.

The invention belongs to the technical field of antibiotic residue detection, and particularly relates to a rapid detection method for quinolone antibiotics in dairy products. The preparation method comprises the following steps: preparing gold nanoparticles as gold seeds by using a sodium citrate reduction method, epitaxially growing an ultrathin Cr-MIL-101-NH2 shell layer by using a layer-by-layer self-assembly method, and constructing an Au-coated Cr-MIL-101-NH2 NPs core-shell structure; a solid phase microextraction-surface enhanced Raman spectroscopy (SPME-SERS) combined method is established on the basis of the material, a shell layer is used for efficiently extracting and selectively enriching a target object to a gold nanoparticle local plasma resonance area, strong SERS signal enhancement is achieved, and the shell layer guarantees that the gold nanoparticles are stable in performance in a complex matrix at the same time. Methodology verification proves that the method is wide in linear range, good in precision, high in recovery rate, low in detection limit, convenient in pretreatment and suitable for rapid high-throughput detection of antibiotic residues in food.

A method for preparing and applying copper chloride-modified vinegar residue biochar, belonging to the field of wastewater treatment technology, is disclosed. The method involves: weighing 1.0000 g ± 0.0005 g of vinegar residue powder and adding it to 40 mL of ultrapure water, dispersing it thoroughly under magnetic stirring; then adding 0.5000 g of CuCl₂·2H₂O, mixing, and continuously stirring magnetically at room temperature for 24 h to allow the CuCl₂ to form a biochar. 2+ The mixture is thoroughly impregnated with vinegar residue to form a mixture. The mixture is then dried in an oven and transferred to a tube furnace. Inside the tube furnace, under a N₂ atmosphere, the temperature is increased to 600°C at 8°C / min and pyrolyzed for 120 min. After cooling, the product is removed, ground, and then copper chloride-modified vinegar residue biochar is obtained. This invention features a simple process flow, controllable operating conditions, low equipment requirements, good repeatability, and suitability for large-scale preparation. The obtained copper chloride-modified vinegar residue biochar exhibits excellent adsorption and degradation performance of organic pollutants, particularly efficient degradation and removal of quinolone antibiotics such as ciprofloxacin and norfloxacin.

The embodiment of the present application provides a Fe3O4@COF electrochemical sensor based on a covalent organic framework, a preparation method and application, the electrochemical sensor comprises: a base electrode which is surface-modified with a solid film; the solid film is a Fe3O4@COF composite material; the Fe3O4@COF composite material comprises: a core formed by a porous structure of Fe3O4 and a COF shell layer attached to the surface of the core; the COF shell layer is a covalent organic framework layer formed by copolymerization of aromatic aldehyde compounds and polyamino aromatic compounds. The Fe3O4@COF electrochemical sensor based on the covalent organic framework, the preparation method and the application can detect quinolone antibiotic molecules such as norfloxacin, and the detection limit can reach 1.927*10 ‑ 13 mol / L; and can be widely applied to the detection of quinolone antibiotics such as norfloxacin in milk and environmental water samples.

The invention discloses a bimodal sensing detection method and a bimodal sensing detection device for quinolone antibiotics. According to the method, two-channel quantitative detection and cross validation are realized on the basis of specific recognition of a nucleic acid aptamer on quinolone antibiotics in combination with a fluorescence and photoelectrochemical double-signal output mode. The device comprises a photoelectric detection module, a fluorescence detection module, a disposable sensing chip slot, a microprocessor control unit, a power supply management module, a man-machine interaction module and a wireless communication module, and all the modules are connected through IC, SPI and UART standardized bus interfaces. And a bimodal signal processing algorithm is built in the device, so that the concentration and consistency score of the target object can be output. The method has the advantages of bimodal verification, high reliability, high sensitivity, strong anti-interference capability, field portability and the like, and is suitable for rapid screening of quinolone antibiotics in complex water bodies such as domestic sewage, hospital wastewater, aquaculture wastewater and the like.

The invention relates to the technical field of bacterial detection, in particular to a primer composition for simultaneously detecting pathogenic bacteria genes and drug-resistant genes, a detection method and a detection device. The core of the invention lies in synchronous detection of two main bacteria causing sepsis, namely burkholderia pseudomallei and klebsiella pneumoniae, and key drug-resistant genes by using a loop-mediated isothermal amplification (LAMP) technology. Specifically, the primer composition comprises nucleotide sequences as shown in SEQ ID No.1-17, and can be used for detecting a burkholderia pseudomallei recA pathogenic gene, a kfB pathogenic gene of klebsiella pneumoniae, a quinolone antibiotic drug-resistant gene qnrA and a carbapenem antibiotic drug-resistant gene OXA-48 in a targeted manner. The primer composition disclosed by the invention can be used for simultaneously detecting various pathogenic bacteria genes and drug-resistant genes causing sepsis, the detection process is convenient, rapid and accurate, the detection result can be obtained within 40 minutes, and the detection effect is better.

The invention belongs to the technical field of biological sewage treatment, and particularly relates to a degrading bacterium and application thereof. The degrading bacterium is Achromobacter sp. Island, is preserved in the China General Microbiological Culture Collection Center on July 22, 2025, has the capability of synchronously degrading high-concentration fluoroquinolone antibiotics and nitrate, and can be directly used for denitrification treatment of wastewater containing high-concentration ciprofloxacin (CIP) in pharmacy, cultivation and the like.

The present application relates to the field of biological enzyme catalyst, especially to a kind of immobilized laccase based on inorganic porous material and its preparation method and application, the immobilized laccase based on inorganic porous material designed in the present application, the immobilized laccase includes inorganic porous material and laccase adsorbed and fixed on the surface of inorganic porous material, the inorganic porous material is prepared by dynamic hydrothermal synthesis method with piperidine as template agent, with aluminum isopropylate as aluminum source, boric acid as boron source, fumed silica as silicon source. Laccase is adsorbed and immobilized by using the inorganic porous material, and the immobilized laccase catalyst obtained has good enzymatic performance, and the immobilized laccase has significant effect in removing quinolone antibiotics.

The invention relates to a method for analyzing pollutants in hospital wastewater based on liquid chromatography-high resolution mass spectrometry. The method comprises the following steps: S1, enriching pollutants in hospital wastewater through solid-phase extraction of four specific solid-phase extraction columns, and then analyzing and detecting a liquid to be detected through a specific chromatographic column, a specific liquid chromatography and a high-resolution mass spectrometry which are connected in series; the identification of various pollutants (medicines and metabolites thereof) in the hospital wastewater is realized, so that a data basis is provided for subsequent fingerprint analysis. Specifically, the analysis method provided by the invention can identify 34 kinds of substances, including sulfonamide antibiotics, macrolide antibiotics, cephalosporin antibiotics, quinolone antibiotics, beta-lactam antibiotics, aminoglycoside antibiotics and carbamazepine and metabolites thereof.

Marine pollution caused by antibiotic abuse has become a global environmental challenge, wherein trace detection of quinolone antibiotics (such as fleroxacin FLE, norfloxacin NOR and enrofloxacin ENR) is particularly key. The invention develops a high-stability fluorescence sensor based on a lanthanide series metal organic framework (Ln-MOF (at) BTEC), and by introducing a hydrogen bond network strengthening strategy, the performance of the material in a seawater high-salt environment is remarkably improved. The fluorescence decay rate of the material in simulated seawater within 60 min is as low as 2.67%, which is 21.49% in 30 min compared with that of a base material, and the stability is obviously improved. In addition, the ratio proportional fluorescence sensing can effectively and instantaneously respond to FLE, NOR and ENR in the presence of numerous interferents in a seawater system, and the detection limits within the range of 0-10 [mu] M are 80.7 nM, 21.03 nM and 28.22 nM respectively. Meanwhile, an intelligent mobile phone platform is successfully integrated to realize visual field detection, and the standard addition recovery rate in Dalian coastal areas and seawater in culture areas reaches 83-115% (RSDlt; the research provides a novel solution with high stability and high sensitivity for marine antibiotic pollution monitoring.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The present invention belongs to the field of microbial degradation technology, and specifically relates to a Pandora bacteria, a bacterial agent and its application in the degradation of quinolone antibiotics. Pandoraea sp.) was named AYGW‑1 and deposited with the China Center for Type Culture Collection on March 10, 2025, with the accession number CCTCC M 2025415. This Pandoracone AYGW‑1 is capable of degrading quinolone antibiotics, providing an effective biodegradation solution for the treatment of quinolone antibiotic pollution in the environment, especially in water. It also offers a safe and environmentally friendly solution to the problem of quinolone antibiotic residues.

The application belongs to the technical field of pollutant detection, and relates to an iron-based nano-enzyme and application thereof in detection of quinolone antibiotics. Trichloride iron hexahydrate and cerium nitrate hexahydrate are added into water and uniformly mixed to obtain a mixed solution A, the mixed solution A is uniformly mixed with an organic polyacid ligand solution, and then a reflux reaction is carried out to prepare a Ce / Fe-MOF precursor. The Ce / Fe-MOF precursor and sodium hypophosphite are added into a tube furnace, and calcination is carried out under a nitrogen atmosphere to prepare Ce / Fe-P. The material synthesized by the application has a mesoporous structure of nanometer prisms, effectively increases the contact area with a substrate, and based on the principle that low-concentration NOR promotes the activity of Ce / Fe-P and high-concentration inhibits the activity of Ce / Fe-P, visual detection of NOR can be realized, and the application has high sensitivity, low detection limit, a wide linear response range, and good selectivity and stability.

The invention discloses quinolone antibiotic drug molecule detection equipment, and relates to the technical field of antibiotic drug molecule detection equipment.The quinolone antibiotic drug molecule detection equipment.The quinolone antibiotic drug molecule detection equipment comprises a case, an opening and closing structure is arranged on the outer wall of the case, and a constant-temperature controller is fixedly connected to the inner wall of the case; a push rod assembly is arranged in the case, a clamping rotating assembly is arranged at the side end of the push rod assembly, a translation assembly is arranged at the other end of the clamping rotating assembly, a mounting column is arranged on the outer wall of the case, a supporting plate is fixedly connected to the side end of the mounting column, and a display screen is fixedly connected to the side end of the supporting plate. The side end of the mounting column is fixedly connected with a keyboard, through cooperative work of the opening and closing structure, the push rod assembly, the clamping rotating assembly and the translation assembly, efficient and accurate detection of medicine molecules is achieved, and the practicability and user friendliness of the equipment are further improved.

The invention relates to the technical field of wastewater treatment, and particularly discloses a phycomycete co-culture system for treating aquaculture wastewater as well as a preparation method and application of the phycomycete co-culture system. The phycomycete co-culture system comprises a quinolone degrading bacterial agent, resistant microalgae, a signal inducer, a porous fixed carrier, a nutrition stimulant and a domestication culture medium; the quinolone degrading bacterial agent is a compound bacterial agent of pseudomonas and acinetobacter, and the resistant microalgae is a compound algae species of chlorella and scenedesmus quadricauda; according to the system, phycomycete symbiotic particles are prepared through gradient domestication culture and immobilization; when in use, the system is put into wastewater, and treatment is carried out under the conditions of illumination and intermittent aeration; through the synergistic effect of functional bacteria and algae compounding, signal induction, composite carrier fixation and nutrition maintenance, the tolerance and degradation efficiency of the system to quinolone antibiotics are remarkably improved, meanwhile, efficient removal of ammonia nitrogen, total phosphorus and COD is achieved, and the algae and algae co-culture system is easy to recycle and has good application prospects.

The invention provides a method for relieving inhibition of quinolone antibiotics on anaerobic digestion based on cow dung biochar, and belongs to the technical field of wastewater treatment. The method comprises the following steps: adding cow dung biochar into a to-be-treated system for carrying out anaerobic digestion on the quinolone antibiotic wastewater by adopting anaerobic sludge; wherein the cow dung biochar is obtained by pyrolyzing cow dung. According to the method, cow dung biochar is added into a to-be-treated system, the cow dung biochar has good adsorption and electrochemical performance and the like, the surface of the cow dung biochar contains a proper amount of iron, the inhibition effect of antibiotics on anaerobic digestion can be well relieved, and the methane yield and the antibiotic removal efficiency of the system can be improved; meanwhile, compared with a nano iron-carbon composite material, the cow dung biochar does not need a complex pretreatment process, and is convenient to operate.

The application discloses a method for efficiently degrading fluoroquinolone antibiotics by using natural mineral materials, and first synthesizes a natural ferrous sulfide mineral; the synthesized ferrous sulfide mineral is added into a fluoroquinolone antibiotic solution, a suitable buffer salt is used to adjust the pH of the reaction, and the degradation reaction of the fluoroquinolone antibiotic is started under uniform stirring in air. The method for oxidatively degrading typical fluoroquinolone antibiotics by using the ferrous sulfide mineral material overcomes the problems of the traditional Fenton system, such as large consumption of raw materials, strong requirement for strong acid, and easy secondary pollution. The synthesized low-concentration ferrous sulfide natural mineral material is used to realize the rapid degradation of typical fluoroquinolone antibiotics, such as enrofloxacin, ciprofloxacin and flumequine, under neutral conditions; and degradation product analysis shows that the reaction path is mainly a step-by-step ring-opening process of a piperazine ring, and a defluorohydroxylation process is a secondary path.

This invention, belonging to the field of chemical analysis, discloses a magnetic hierarchical porous NH2-UiO-66 material and its application in the determination of quinolone antibiotics in animal foods. The invention first prepares a metal-organic framework material (hierarchical porous NH2-UiO-66 material) with a hierarchical porous structure using molecular imprinting technology. This metal-organic framework material is then imbued with magnetic properties by treating it with trivalent iron salts, sodium citrate, and sodium acetate, resulting in the magnetic hierarchical porous NH2-UiO-66 material. The magnetic graded porous NH2-UiO-66 material prepared by the present invention is used for the extraction, separation, enrichment and detection of quinolone antibiotics in animal foods, achieving a high extraction rate (95.12-97.92%), a good linear range (5-500 μg / L), a low detection limit (milk: 0.16-1.18 μg / L, pork: 0.34-2.16 μg / L) and a low limit of quantification (milk: 0.54-3.95 μg / L, pork: 1.15-3.55 μg / L), as well as a satisfactory recovery rate (milk: 70.15-109.67%, pork: 71.48-93.84%). It can be used for the highly sensitive detection of quinolone antibiotics in complex matrix foods.

The invention discloses a method for detecting quinoxaline antibiotics and main metabolites thereof, and relates to the field of agriculture and forestry chemistry detection.The method comprises the following steps that 1, a UCNPs-mAb fluorescent probe is prepared; step 2, preparing and assembling UCNPs-LFSBs; step 3, optimizing a UCNPs-LFSBs system, wherein the optimization content comprises the antibody labeling amount, the concentration of OLA-OVA, QCT-OVA and MQCA-BSA on a T line, the concentration of GAM-IgG on a C line, the mixing ratio of UCNPs-mAb mixed probes on a conjugate pad, the artificial antigen membrane scratching position, a buffer solution and the reaction time; by means of the optimized detection method, OLA, QCT and MQCA can be rapidly and quantitatively measured at the same time with high sensitivity.