The invention discloses a method for removing fluoroquinolones antibiotics in poultry and livestock excrement. The method includes the steps of 1, preparing compost raw materials, wherein chicken excrement, sawdust, sterile distilled water and high-temperature-resistant fungicide are prepared into the compost raw materials, the mass ratio of the chicken excrement to the sawdust is 5-10 to 1, the water content of the compost raw materials ranges from 55% to 60%, and the high-temperature-resistant fungicide is cellulomonas flavigena; 2, carrying out high-temperature composting, wherein the pile overturning frequency is controlled to enable the compost temperature to range from 45 DEG C to 65 DEG C, and high-temperature fermentation is achieved; 3, carrying out intermediate-temperature composting, wherein after high-temperature fermentation, the manual pile overturning frequency is controlled to enable the temperature of a compost product to range from 30 DEG C to 45 DEG C, and intermediate-temperature fermentation is achieved. According to the method, the fluoroquinolones antibiotics in the poultry and livestock excrement are removed sufficiently through the degradation effect of high-temperature-resistant strains and relative microorganisms, and therefore the fluoroquinolones antibiotics in organic fertilizers are reduced to the safe level.