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Rapid detection method and rapid detection kit of fluoroquinolone antibiotics

A technology of fluoroquinolones and detection methods, applied in the field of drug detection, can solve the problems of no formal inspection standards, promulgation, and no relevant literature reports, etc., and achieve strong anti-interference ability of coexisting substances, low detection cost, and strong specificity Effect

Inactive Publication Date: 2010-04-07
广东省汕尾市药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the literature review and investigation, there are no relevant literature reports on the detection methods of Chinese patent medicines and health foods for the treatment of prostate diseases and illegally added chemical drugs, and no formal inspection standards have been promulgated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In this example, the rapid detection of fluoroquinolone antibiotics added to Jinliebao Xiongjian Capsules includes the following steps:

[0033] (1) acid extraction:

[0034] Take one Jinliebao Xiongjian Capsule, grind it well, add 2ml 2% hydrochloric acid solution by volume, shake and extract for 30 seconds, let it stand, take the upper layer extract, filter it with a filter membrane, and collect the filtrate; because the filtrate is darker in color, brownish yellow, So in this filtrate, dropwise add 0.04mol / l potassium permanganate solution, shake while adding dropwise, until the filtrate is pale yellow;

[0035] (2) Color reaction

[0036] Add 1-2 drops of 9% by mass ferric chloride solution dropwise to the above-mentioned treated filtrate to carry out color reaction, the color reaction time is immediate color development, and the color development result is observed.

[0037] In this example, after the ferric chloride solution was added dropwise to the filtrate, t...

Embodiment 2

[0039] In this embodiment, the rapid detection of fluoroquinolone antibiotics added in Qianlie Huichun Tablets includes the following steps:

[0040] (1) acid extraction:

[0041] Take a piece of Qianlie Huichun Tablet, grind it evenly, add 2ml 2% hydrochloric acid solution by volume, shake and extract for 30 seconds, place it, take the upper layer extract, filter through a filter membrane, and collect the filtrate; because the filtrate is darker and brownish yellow, so add it to the Add 0.04mol / l potassium permanganate solution dropwise to the filtrate, and shake well while adding dropwise, until the filtrate is pale yellow;

[0042] (2) Color reaction

[0043]Add 1-2 drops of 9% by mass ferric chloride solution dropwise to the above-mentioned treated filtrate to carry out color reaction, the color reaction time is immediate color development, and the color development result is observed.

[0044] In the present embodiment, after the ferric chloride solution was added dropw...

Embodiment 3

[0045] Embodiment 3 specific detection

[0046] This embodiment selects amoxicillin, roxithromycin, cephalexin, penicillin V potassium, clotrimazole, melelamycin, tetracycline, oxytetracycline, promethazine hydrochloride, chlorpromazine hydrochloride, norfloxacin and these antibiotic drugs ofloxacin, adopt the rapid detection method described in embodiment 1, the specificity of this method is tested.

[0047] The color development results of each antibiotic are as follows:

[0048] 1. The color results of amoxicillin, roxithromycin, cephalexin, penicillin V potassium, clotrimazole and mebelamycin are all slightly yellow, without interference, and are negative results;

[0049] 2. The color results of tetracycline and oxytetracycline are brown, and there is interference, but after dilution, the interference can be eliminated by comparing with the positive tube, which is a negative result;

[0050] 3. Promethazine hydrochloride and chlorpromazine hydrochloride show pink interf...

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PUM

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Abstract

The invention discloses a rapid detection method and a rapid detection kit of fluoroquinolone antibiotics added in drugs, health care products and foods. The method comprises the following steps: utilizing acid solution to carry out extracting treatment on drugs, health care products and foods, filtering the extracting solution and then carrying out fading process with potassium permanganate solution, adding ferric trichloride solution for coloration, and judging according to the coloration result. The kit of the invention comprises acid solution, potassium permanganate solution, ferric trichloride solution, strainer head, injector and positive control. The method of the invention has low analysis cost, strong ability of resisting interference of coexisting substances, no need for special equipment, high sensitivity, obvious and easily judged coloration result and high accuracy of detection conclusion, and is suitable for rapidly detecting whether fluoroquinolone antibiotics are added in drugs, health care products and foods. The rapid detection kit can be prepared according to the rapid detection method of the invention, thereby meeting the demand for developing food and drug supervision detection in rural areas.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to the field of detection of illegally added antibiotics in medicines, health products or food, and in particular to a rapid detection method and rapid detection kit for adding fluoroquinolone antibiotics to medicines, health products or food. Background technique [0002] Traditional Chinese medicine is a great treasure house of our country. It has the great advantages of being pure and natural, with few or even no toxic and side effects. Chinese patent medicine and its health food are directly processed from Chinese herbal medicine, which is convenient for patients to take. The traditional Chinese medicine has less toxic and side effects within the prescribed dose. . However, in recent years, some unscrupulous companies or drug dealers have taken advantage of the current lack of strict quality control standards in Chinese patent medicines and health foods, and secretly mixed chemical...

Claims

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Application Information

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IPC IPC(8): G01N31/22
Inventor 洪美华陈伟军陈志强蔡诗填温小霞
Owner 广东省汕尾市药品检验所
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